CH617963A5 - - Google Patents
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- Publication number
- CH617963A5 CH617963A5 CH1426573A CH1426573A CH617963A5 CH 617963 A5 CH617963 A5 CH 617963A5 CH 1426573 A CH1426573 A CH 1426573A CH 1426573 A CH1426573 A CH 1426573A CH 617963 A5 CH617963 A5 CH 617963A5
- Authority
- CH
- Switzerland
- Prior art keywords
- process according
- proteolytic enzyme
- inhibitor
- protease inhibitor
- filtrate
- Prior art date
Links
- 238000000034 method Methods 0.000 claims description 27
- 210000000056 organ Anatomy 0.000 claims description 18
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 claims description 16
- 239000003112 inhibitor Substances 0.000 claims description 16
- 239000000137 peptide hydrolase inhibitor Substances 0.000 claims description 16
- 102000035195 Peptidases Human genes 0.000 claims description 14
- 108091005804 Peptidases Proteins 0.000 claims description 14
- 239000004365 Protease Substances 0.000 claims description 14
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims description 13
- 238000000605 extraction Methods 0.000 claims description 13
- 229920000669 heparin Polymers 0.000 claims description 13
- 229960002897 heparin Drugs 0.000 claims description 13
- 210000004072 lung Anatomy 0.000 claims description 13
- 210000001557 animal structure Anatomy 0.000 claims description 9
- 239000000706 filtrate Substances 0.000 claims description 9
- 241000283690 Bos taurus Species 0.000 claims description 7
- 108090000526 Papain Proteins 0.000 claims description 7
- 235000019834 papain Nutrition 0.000 claims description 7
- 229940055729 papain Drugs 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 6
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 claims description 6
- 108010004032 Bromelains Proteins 0.000 claims description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 5
- 239000001166 ammonium sulphate Substances 0.000 claims description 5
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 5
- 235000019835 bromelain Nutrition 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 125000001453 quaternary ammonium group Chemical group 0.000 claims description 5
- 239000008135 aqueous vehicle Substances 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- 239000006166 lysate Substances 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims description 4
- 108060005987 Kallikrein Proteins 0.000 claims description 3
- 102000001399 Kallikrein Human genes 0.000 claims description 3
- 108090000631 Trypsin Proteins 0.000 claims description 3
- 102000004142 Trypsin Human genes 0.000 claims description 3
- NEUSVAOJNUQRTM-UHFFFAOYSA-N cetylpyridinium Chemical group CCCCCCCCCCCCCCCC[N+]1=CC=CC=C1 NEUSVAOJNUQRTM-UHFFFAOYSA-N 0.000 claims description 3
- 229960004830 cetylpyridinium Drugs 0.000 claims description 3
- 239000012588 trypsin Substances 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 238000000227 grinding Methods 0.000 claims description 2
- 230000037452 priming Effects 0.000 claims 1
- AISMNBXOJRHCIA-UHFFFAOYSA-N trimethylazanium;bromide Chemical compound Br.CN(C)C AISMNBXOJRHCIA-UHFFFAOYSA-N 0.000 claims 1
- 230000000694 effects Effects 0.000 description 9
- 238000000746 purification Methods 0.000 description 9
- 239000007788 liquid Substances 0.000 description 8
- 239000002244 precipitate Substances 0.000 description 7
- 239000012264 purified product Substances 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 6
- 239000012043 crude product Substances 0.000 description 4
- 230000009089 cytolysis Effects 0.000 description 4
- 238000005194 fractionation Methods 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 3
- 239000001110 calcium chloride Substances 0.000 description 3
- 229910001628 calcium chloride Inorganic materials 0.000 description 3
- 239000003599 detergent Substances 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 108090000270 Ficain Proteins 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 108090000284 Pepsin A Proteins 0.000 description 2
- 102000057297 Pepsin A Human genes 0.000 description 2
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 125000002091 cationic group Chemical group 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 235000019836 ficin Nutrition 0.000 description 2
- POTUGHMKJGOKRI-UHFFFAOYSA-N ficin Chemical compound FI=CI=N POTUGHMKJGOKRI-UHFFFAOYSA-N 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 210000000496 pancreas Anatomy 0.000 description 2
- 229940111202 pepsin Drugs 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 229960001322 trypsin Drugs 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 206010002198 Anaphylactic reaction Diseases 0.000 description 1
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
- 102000003670 Carboxypeptidase B Human genes 0.000 description 1
- 108090000087 Carboxypeptidase B Proteins 0.000 description 1
- 102000000496 Carboxypeptidases A Human genes 0.000 description 1
- 108010080937 Carboxypeptidases A Proteins 0.000 description 1
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
- 108090000317 Chymotrypsin Proteins 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 108010067372 Pancreatic elastase Proteins 0.000 description 1
- 102000016387 Pancreatic elastase Human genes 0.000 description 1
- 108010019160 Pancreatin Proteins 0.000 description 1
- 206010033645 Pancreatitis Diseases 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000002052 anaphylactic effect Effects 0.000 description 1
- 230000036783 anaphylactic response Effects 0.000 description 1
- 208000003455 anaphylaxis Diseases 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 239000008365 aqueous carrier Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 235000010633 broth Nutrition 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229960002376 chymotrypsin Drugs 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 238000001641 gel filtration chromatography Methods 0.000 description 1
- 238000005227 gel permeation chromatography Methods 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 239000002045 hydroalcoholic fraction Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000005461 lubrication Methods 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 229940055695 pancreatin Drugs 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- -1 plasmine Proteins 0.000 description 1
- 229920000447 polyanionic polymer Polymers 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000002510 pyrogen Substances 0.000 description 1
- 230000001698 pyrogenic effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000009938 salting Methods 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0066—Isolation or extraction of proteoglycans from organs
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
- C07K14/8114—Kunitz type inhibitors
- C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Polymers & Plastics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Materials Engineering (AREA)
- Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Compounds Of Unknown Constitution (AREA)
Priority Applications (12)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH1426573A CH617963A5 (el) | 1973-10-05 | 1973-10-05 | |
| JP49046059A JPS619286B2 (el) | 1973-10-05 | 1974-04-25 | |
| DD181437A DD118654A1 (el) | 1973-10-05 | 1974-10-01 | |
| DE2447050A DE2447050C2 (de) | 1973-10-05 | 1974-10-02 | Verfahren zur Gewinnung von Proteasenhemmstoff |
| SU742068151A SU611595A3 (ru) | 1973-10-05 | 1974-10-04 | Способ одновременного получени ингибитора протеазы и гепарина |
| ES430685A ES430685A1 (es) | 1973-10-05 | 1974-10-04 | Metodo para la produccion de inhibidor de proteasa, hepari-na y otros mucopolisacaridos. |
| NL7413127A NL7413127A (nl) | 1973-10-05 | 1974-10-04 | Werkwijze voor het bereiden van een remmer roteasen. |
| AR255931A AR200699A1 (es) | 1973-10-05 | 1974-10-04 | Metodo para la produccion de inhibidor de proteasa |
| DK522174AA DK139913B (da) | 1973-10-05 | 1974-10-04 | Fremgangsmåde til udvinding af kallikrein-trypsin-inhibitor og eventuelt heparin. |
| CA210,749A CA1034495A (en) | 1973-10-05 | 1974-10-04 | Method for the preparation of protease-inhibitor |
| FR7433572A FR2246567B1 (el) | 1973-10-05 | 1974-10-04 | |
| US05/916,536 US4216293A (en) | 1973-10-05 | 1978-06-19 | Extracting protease-inhibitor from animal tissue containing same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH1426573A CH617963A5 (el) | 1973-10-05 | 1973-10-05 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CH617963A5 true CH617963A5 (el) | 1980-06-30 |
Family
ID=4399389
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CH1426573A CH617963A5 (el) | 1973-10-05 | 1973-10-05 |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US4216293A (el) |
| JP (1) | JPS619286B2 (el) |
| AR (1) | AR200699A1 (el) |
| CA (1) | CA1034495A (el) |
| CH (1) | CH617963A5 (el) |
| DD (1) | DD118654A1 (el) |
| DE (1) | DE2447050C2 (el) |
| DK (1) | DK139913B (el) |
| ES (1) | ES430685A1 (el) |
| FR (1) | FR2246567B1 (el) |
| NL (1) | NL7413127A (el) |
| SU (1) | SU611595A3 (el) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2509482C2 (de) * | 1975-03-05 | 1985-11-21 | Bayer Ag, 5090 Leverkusen | Verfahren zur Herstellung des Kallikrein-Trypsin-Inhibitors aus Rinderlunge |
| JPS5334911A (en) * | 1976-09-01 | 1978-03-31 | Mochida Pharm Co Ltd | Production of proteolytic enzyme inhibitor |
| IT1190313B (it) * | 1986-04-17 | 1988-02-16 | Crinos Industria Farmaco | Procedimento per l'ottenimento di polidesossiribonucleotidi chimicamente definiti e riproducibili e prodotto farmacologicamente attivo risultante |
| CZ299281B6 (cs) | 1997-07-16 | 2008-06-04 | N.V. Organon | Zpusob prípravy heparinu |
| WO2006121803A1 (en) | 2005-05-05 | 2006-11-16 | Sensient Flavors Inc. | Production of beta-glucans and mannans |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3451996A (en) * | 1968-02-12 | 1969-06-24 | Thompson Farms Co | Method for the preparation of heparin |
| SU377159A1 (ru) | 1970-12-31 | 1973-04-17 | Способ получения ингибитора протеаз |
-
1973
- 1973-10-05 CH CH1426573A patent/CH617963A5/it not_active IP Right Cessation
-
1974
- 1974-04-25 JP JP49046059A patent/JPS619286B2/ja not_active Expired
- 1974-10-01 DD DD181437A patent/DD118654A1/xx unknown
- 1974-10-02 DE DE2447050A patent/DE2447050C2/de not_active Expired
- 1974-10-04 SU SU742068151A patent/SU611595A3/ru active
- 1974-10-04 ES ES430685A patent/ES430685A1/es not_active Expired
- 1974-10-04 CA CA210,749A patent/CA1034495A/en not_active Expired
- 1974-10-04 DK DK522174AA patent/DK139913B/da not_active IP Right Cessation
- 1974-10-04 FR FR7433572A patent/FR2246567B1/fr not_active Expired
- 1974-10-04 NL NL7413127A patent/NL7413127A/xx not_active Application Discontinuation
- 1974-10-04 AR AR255931A patent/AR200699A1/es active
-
1978
- 1978-06-19 US US05/916,536 patent/US4216293A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| ES430685A1 (es) | 1976-11-01 |
| DK139913B (da) | 1979-05-14 |
| NL7413127A (nl) | 1975-04-08 |
| US4216293A (en) | 1980-08-05 |
| DK139913C (el) | 1979-10-15 |
| DK522174A (el) | 1975-06-09 |
| AR200699A1 (es) | 1974-11-29 |
| SU611595A3 (ru) | 1978-06-15 |
| DD118654A1 (el) | 1976-03-12 |
| JPS5063186A (el) | 1975-05-29 |
| FR2246567A1 (el) | 1975-05-02 |
| FR2246567B1 (el) | 1978-03-24 |
| CA1034495A (en) | 1978-07-11 |
| DE2447050C2 (de) | 1983-07-28 |
| DE2447050A1 (de) | 1975-04-10 |
| JPS619286B2 (el) | 1986-03-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PL | Patent ceased |