CA3242405A1 - Fusion polypeptides with deamidase inhibitor and deamidase domains - Google Patents

Fusion polypeptides with deamidase inhibitor and deamidase domains

Info

Publication number
CA3242405A1
CA3242405A1 CA3242405A CA3242405A CA3242405A1 CA 3242405 A1 CA3242405 A1 CA 3242405A1 CA 3242405 A CA3242405 A CA 3242405A CA 3242405 A CA3242405 A CA 3242405A CA 3242405 A1 CA3242405 A1 CA 3242405A1
Authority
CA
Canada
Prior art keywords
desamidase
inhibitors
fusion polypeptides
domain fusion
polypeptides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3242405A
Other languages
English (en)
French (fr)
Inventor
Jens Magnus Ekloef
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP22160942.3A external-priority patent/EP4242303A1/en
Application filed by Novozymes AS filed Critical Novozymes AS
Publication of CA3242405A1 publication Critical patent/CA3242405A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01044—Protein-glutamine glutaminase (3.5.1.44)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA3242405A 2022-03-08 2023-03-08 Fusion polypeptides with deamidase inhibitor and deamidase domains Pending CA3242405A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP22160942.3 2022-03-08
EP22160942.3A EP4242303A1 (en) 2022-03-08 2022-03-08 Fusion polypeptides with deamidase inhibitor and deamidase domains
EP22183793 2022-07-08
EP22183793.3 2022-07-08
PCT/EP2023/055936 WO2023170177A1 (en) 2022-03-08 2023-03-08 Fusion polypeptides with deamidase inhibitor and deamidase domains

Publications (1)

Publication Number Publication Date
CA3242405A1 true CA3242405A1 (en) 2023-09-14

Family

ID=85477826

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3242405A Pending CA3242405A1 (en) 2022-03-08 2023-03-08 Fusion polypeptides with deamidase inhibitor and deamidase domains

Country Status (6)

Country Link
US (1) US20250215412A1 (enExample)
EP (1) EP4490286A1 (enExample)
JP (1) JP2025509233A (enExample)
AU (1) AU2023231428A1 (enExample)
CA (1) CA3242405A1 (enExample)
WO (1) WO2023170177A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20250188435A1 (en) * 2022-03-09 2025-06-12 Amano Enzyme Inc. Modified protein glutaminase
AU2024289271A1 (en) 2023-07-05 2025-11-27 Novozymes A/S Foamable dairy products
WO2025132872A1 (en) 2023-12-20 2025-06-26 Novozymes A/S Modified leguminous protein with improved colloidal stability
WO2025233391A1 (en) 2024-05-09 2025-11-13 Novozymes A/S Stabilized liquid deamidase compositions
WO2025233389A1 (en) 2024-05-09 2025-11-13 Novozymes A/S Stabilized liquid deamidase compositions
WO2026060197A1 (en) * 2024-09-13 2026-03-19 International N&H Denmark Aps Novel protein glutaminase for plant based beverages
WO2026073574A1 (en) 2024-10-01 2026-04-09 Novozymes A/S Codon optimized deamidase expression
WO2026076952A1 (en) 2024-10-08 2026-04-16 Novozymes A/S Processes for obtaining dairy protein-based ready-to-drink beverages

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4016040A (en) 1969-12-10 1977-04-05 Colgate-Palmolive Company Preparation of enzyme-containing beads
GB1483591A (en) 1973-07-23 1977-08-24 Novo Industri As Process for coating water soluble or water dispersible particles by means of the fluid bed technique
GB1590432A (en) 1976-07-07 1981-06-03 Novo Industri As Process for the production of an enzyme granulate and the enzyme granuate thus produced
DK263584D0 (da) 1984-05-29 1984-05-29 Novo Industri As Enzymholdige granulater anvendt som detergentadditiver
US4713245A (en) 1984-06-04 1987-12-15 Mitsui Toatsu Chemicals, Incorporated Granule containing physiologically-active substance, method for preparing same and use thereof
EG18543A (en) 1986-02-20 1993-07-30 Albright & Wilson Protected enzyme systems
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
DK435587D0 (da) 1987-08-21 1987-08-21 Novo Industri As Fremgangsmaade til fremstilling af et enzymholdigt granulat
DK435687D0 (da) 1987-08-21 1987-08-21 Novo Industri As Enzymholdigt granulat og fremgangsmaade til fremstilling deraf
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
DK78189D0 (da) 1989-02-20 1989-02-20 Novo Industri As Enzymholdigt granulat og fremgangsmaade til fremstilling deraf
DK78089D0 (da) 1989-02-20 1989-02-20 Novo Industri As Detergentholdigt granulat og fremgangsmaade til fremstilling deraf
EP0477280B1 (en) 1989-06-13 1996-09-11 Genencor International, Inc. A method for killing cells without cell lysis
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
US5879920A (en) 1991-10-07 1999-03-09 Genencor International, Inc. Coated enzyme-containing granule
AU677166B2 (en) 1991-10-07 1997-04-17 Genencor International, Inc. Coated enzyme containing granule
FR2704860B1 (fr) 1993-05-05 1995-07-13 Pasteur Institut Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire.
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
WO1997039116A1 (en) 1996-04-12 1997-10-23 Novo Nordisk A/S Enzyme-containing granules and process for the production thereof
KR20010033321A (ko) 1997-12-20 2001-04-25 마가렛 에이.혼 수화된 차단 재료를 가지는 과립
EP1092007B1 (en) 1998-06-30 2003-11-26 Novozymes A/S A new improved enzyme containing granule
JP2003520571A (ja) 1999-06-02 2003-07-08 ノボザイムス アクティーゼルスカブ ポリペプチドの発現及び分泌のためのペクチン酸リアーゼ
AU2003223928A1 (en) 2002-05-07 2003-11-11 Novozymes A/S Homologous recombination into bacterium for the generation of polynucleotide libraries
CN101056540A (zh) 2004-09-27 2007-10-17 诺维信公司 酶颗粒
RU2560424C2 (ru) 2009-02-20 2015-08-20 ДАНИСКО ЮЭс ИНК. Способ получения состава ферментационного бульона
EP2674475A1 (en) 2012-06-11 2013-12-18 The Procter & Gamble Company Detergent composition
JP6511400B2 (ja) 2013-02-05 2019-05-15 オートリー エービー 液状のエンバクベース
WO2014206806A1 (en) 2013-06-24 2014-12-31 Novozymes A/S Codon modified amylase from bacillus akibai
EP3019607B2 (en) 2013-07-12 2022-09-07 Novozymes A/S Direct transfer of polynucleotides between genomes
CN106795507A (zh) 2014-10-30 2017-05-31 诺维信公司 蛋白酶变体以及对其进行编码的多核苷酸
DE102016002322A1 (de) 2016-02-26 2017-08-31 Hüseyin Keskin Fahr- und/oder Flugsimulator
EP3481959A1 (en) 2016-07-06 2019-05-15 Novozymes A/S Improving a microorganism by crispr-inhibition
CN111434770B (zh) * 2019-01-11 2022-12-06 华东师范大学 蛋白质谷氨酰胺酶酶原在枯草芽孢杆菌中的表达和自加工
WO2021049591A1 (ja) 2019-09-12 2021-03-18 天野エンザイム株式会社 植物タンパク質濃縮物の製造方法

Also Published As

Publication number Publication date
AU2023231428A1 (en) 2024-07-11
WO2023170177A1 (en) 2023-09-14
US20250215412A1 (en) 2025-07-03
JP2025509233A (ja) 2025-04-11
EP4490286A1 (en) 2025-01-15
WO2023170177A8 (en) 2023-11-09

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