CA3228406A1 - In situ epitranscriptomic profiling - Google Patents

In situ epitranscriptomic profiling Download PDF

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Publication number
CA3228406A1
CA3228406A1 CA3228406A CA3228406A CA3228406A1 CA 3228406 A1 CA3228406 A1 CA 3228406A1 CA 3228406 A CA3228406 A CA 3228406A CA 3228406 A CA3228406 A CA 3228406A CA 3228406 A1 CA3228406 A1 CA 3228406A1
Authority
CA
Canada
Prior art keywords
probe
rna
complementary
oligonucleotide
probes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3228406A
Other languages
English (en)
French (fr)
Inventor
Xiao Wang
Hu ZENG
Jingyi Ren
Jiakun TIAN
Jianting GUO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Massachusetts Institute of Technology
Broad Institute Inc
Original Assignee
Massachusetts Institute of Technology
Broad Institute Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Institute of Technology, Broad Institute Inc filed Critical Massachusetts Institute of Technology
Publication of CA3228406A1 publication Critical patent/CA3228406A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA3228406A 2021-08-10 2022-08-10 In situ epitranscriptomic profiling Pending CA3228406A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163231585P 2021-08-10 2021-08-10
US63/231,585 2021-08-10
PCT/US2022/039895 WO2023018756A1 (en) 2021-08-10 2022-08-10 In situ epitranscriptomic profiling

Publications (1)

Publication Number Publication Date
CA3228406A1 true CA3228406A1 (en) 2023-02-16

Family

ID=83151401

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3228406A Pending CA3228406A1 (en) 2021-08-10 2022-08-10 In situ epitranscriptomic profiling

Country Status (7)

Country Link
EP (1) EP4384635A1 (https=)
JP (1) JP2024531957A (https=)
KR (1) KR20240046547A (https=)
CN (1) CN118076749A (https=)
AU (1) AU2022326458A1 (https=)
CA (1) CA3228406A1 (https=)
WO (1) WO2023018756A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7502787B2 (ja) 2018-04-09 2024-06-19 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー インサイチュ遺伝子配列決定の方法
WO2025059443A1 (en) 2023-09-15 2025-03-20 The Broad Institute, Inc. Untargeted multiplexed in situ rna profiling and the uses and means therefor
WO2025136916A1 (en) 2023-12-18 2025-06-26 The Board Institute, Inc. Thick tissue transcriptomics and translatomics

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080124735A1 (en) * 2006-10-16 2008-05-29 Matthias Schuster Method for detection of one or more CpG positions
US10597701B2 (en) * 2011-05-11 2020-03-24 Navinci Diagnostics Ab Unfolding proximity probes and methods for the use thereof
US11268144B2 (en) * 2015-09-24 2022-03-08 Sigma-Aldrich Co. Llc Methods and reagents for molecular proximity detection using RNA-guided nucleic acid binding proteins
JP7502787B2 (ja) 2018-04-09 2024-06-19 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー インサイチュ遺伝子配列決定の方法
US12110548B2 (en) * 2020-02-03 2024-10-08 10X Genomics, Inc. Bi-directional in situ analysis

Also Published As

Publication number Publication date
EP4384635A1 (en) 2024-06-19
JP2024531957A (ja) 2024-09-03
KR20240046547A (ko) 2024-04-09
AU2022326458A1 (en) 2024-02-22
CN118076749A (zh) 2024-05-24
WO2023018756A1 (en) 2023-02-16

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