KR20240046547A - 계내 에피트랜스크립톰 프로파일링 - Google Patents

계내 에피트랜스크립톰 프로파일링 Download PDF

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Publication number
KR20240046547A
KR20240046547A KR1020247007861A KR20247007861A KR20240046547A KR 20240046547 A KR20240046547 A KR 20240046547A KR 1020247007861 A KR1020247007861 A KR 1020247007861A KR 20247007861 A KR20247007861 A KR 20247007861A KR 20240046547 A KR20240046547 A KR 20240046547A
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South Korea
Prior art keywords
probe
rna
complementary
oligonucleotide
epitranscriptome
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Pending
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KR1020247007861A
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English (en)
Korean (ko)
Inventor
샤오 왕
후 쩡
징이 런
자쿤 톈
졘팅 궈
?컷? 궈
Original Assignee
더 브로드 인스티튜트, 인코퍼레이티드
메사추세츠 인스티튜트 오브 테크놀로지
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Publication of KR20240046547A publication Critical patent/KR20240046547A/ko
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
KR1020247007861A 2021-08-10 2022-08-10 계내 에피트랜스크립톰 프로파일링 Pending KR20240046547A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163231585P 2021-08-10 2021-08-10
US63/231,585 2021-08-10
PCT/US2022/039895 WO2023018756A1 (en) 2021-08-10 2022-08-10 In situ epitranscriptomic profiling

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KR20240046547A true KR20240046547A (ko) 2024-04-09

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KR1020247007861A Pending KR20240046547A (ko) 2021-08-10 2022-08-10 계내 에피트랜스크립톰 프로파일링

Country Status (7)

Country Link
EP (1) EP4384635A1 (https=)
JP (1) JP2024531957A (https=)
KR (1) KR20240046547A (https=)
CN (1) CN118076749A (https=)
AU (1) AU2022326458A1 (https=)
CA (1) CA3228406A1 (https=)
WO (1) WO2023018756A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7502787B2 (ja) 2018-04-09 2024-06-19 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー インサイチュ遺伝子配列決定の方法
WO2025059443A1 (en) 2023-09-15 2025-03-20 The Broad Institute, Inc. Untargeted multiplexed in situ rna profiling and the uses and means therefor
WO2025136916A1 (en) 2023-12-18 2025-06-26 The Board Institute, Inc. Thick tissue transcriptomics and translatomics

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080124735A1 (en) * 2006-10-16 2008-05-29 Matthias Schuster Method for detection of one or more CpG positions
US10597701B2 (en) * 2011-05-11 2020-03-24 Navinci Diagnostics Ab Unfolding proximity probes and methods for the use thereof
US11268144B2 (en) * 2015-09-24 2022-03-08 Sigma-Aldrich Co. Llc Methods and reagents for molecular proximity detection using RNA-guided nucleic acid binding proteins
JP7502787B2 (ja) 2018-04-09 2024-06-19 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー インサイチュ遺伝子配列決定の方法
US12110548B2 (en) * 2020-02-03 2024-10-08 10X Genomics, Inc. Bi-directional in situ analysis

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EP4384635A1 (en) 2024-06-19
JP2024531957A (ja) 2024-09-03
CA3228406A1 (en) 2023-02-16
AU2022326458A1 (en) 2024-02-22
CN118076749A (zh) 2024-05-24
WO2023018756A1 (en) 2023-02-16

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