CA3222023A1 - Gene editing systems comprising a crispr nuclease and uses thereof - Google Patents

Gene editing systems comprising a crispr nuclease and uses thereof Download PDF

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Publication number
CA3222023A1
CA3222023A1 CA3222023A CA3222023A CA3222023A1 CA 3222023 A1 CA3222023 A1 CA 3222023A1 CA 3222023 A CA3222023 A CA 3222023A CA 3222023 A CA3222023 A CA 3222023A CA 3222023 A1 CA3222023 A1 CA 3222023A1
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Prior art keywords
nucleotides
sequence
gene editing
rna
polypeptide
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CA3222023A
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English (en)
French (fr)
Inventor
David A. Scott
Noah Michael Jakimo
Pratyusha HUNNEWELL
Fu-Kai HSIEH
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Arbor Biotechnologies Inc
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Arbor Biotechnologies Inc
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
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    • C07K2319/00—Fusion polypeptide
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
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  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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CA3222023A 2021-06-01 2022-06-01 Gene editing systems comprising a crispr nuclease and uses thereof Pending CA3222023A1 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US202163195621P 2021-06-01 2021-06-01
US63/195,621 2021-06-01
US202163236047P 2021-08-23 2021-08-23
US63/236,047 2021-08-23
US202163272937P 2021-10-28 2021-10-28
US63/272,937 2021-10-28
US202263299695P 2022-01-14 2022-01-14
US63/299,695 2022-01-14
PCT/US2022/031821 WO2022256440A2 (en) 2021-06-01 2022-06-01 Gene editing systems comprising a crispr nuclease and uses thereof

Publications (1)

Publication Number Publication Date
CA3222023A1 true CA3222023A1 (en) 2022-12-08

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CA3222023A Pending CA3222023A1 (en) 2021-06-01 2022-06-01 Gene editing systems comprising a crispr nuclease and uses thereof

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US (2) US20230023791A1 (https=)
EP (1) EP4347818A2 (https=)
JP (1) JP2024520528A (https=)
KR (1) KR20240031238A (https=)
AU (1) AU2022284804A1 (https=)
BR (1) BR112023024985A2 (https=)
CA (1) CA3222023A1 (https=)
IL (1) IL308806A (https=)
TW (1) TW202313971A (https=)
WO (1) WO2022256440A2 (https=)

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