CA3206049A1 - Methods for expanding regulatory t cells - Google Patents

Methods for expanding regulatory t cells Download PDF

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Publication number
CA3206049A1
CA3206049A1 CA3206049A CA3206049A CA3206049A1 CA 3206049 A1 CA3206049 A1 CA 3206049A1 CA 3206049 A CA3206049 A CA 3206049A CA 3206049 A CA3206049 A CA 3206049A CA 3206049 A1 CA3206049 A1 CA 3206049A1
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CA
Canada
Prior art keywords
tregs
population
aspects
days
stimulating
Prior art date
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Pending
Application number
CA3206049A
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English (en)
French (fr)
Inventor
John Cho
Kerem Jonatan TUNCEL
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KSQ Therapeutics Inc
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KSQ Therapeutics Inc
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Publication of CA3206049A1 publication Critical patent/CA3206049A1/en
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • C12N5/0637—Immunosuppressive T lymphocytes, e.g. regulatory T cells or Treg
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/11—T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/20—Cellular immunotherapy characterised by the effect or the function of the cells
    • A61K40/22—Immunosuppressive or immunotolerising
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/416—Antigens related to auto-immune diseases; Preparations to induce self-tolerance
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/51—B7 molecules, e.g. CD80, CD86, CD28 (ligand), CD152 (ligand)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/515—CD3, T-cell receptor complex
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/53—CD2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2510/00—Genetically modified cells

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Medicinal Chemistry (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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CA3206049A 2021-02-23 2022-02-23 Methods for expanding regulatory t cells Pending CA3206049A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163152787P 2021-02-23 2021-02-23
US63/152,787 2021-02-23
PCT/US2022/017526 WO2022182763A1 (en) 2021-02-23 2022-02-23 Methods for expanding regulatory t cells

Publications (1)

Publication Number Publication Date
CA3206049A1 true CA3206049A1 (en) 2022-09-01

Family

ID=83048425

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3206049A Pending CA3206049A1 (en) 2021-02-23 2022-02-23 Methods for expanding regulatory t cells

Country Status (7)

Country Link
US (1) US20240228962A9 (enExample)
EP (1) EP4297757A4 (enExample)
JP (1) JP2024507373A (enExample)
CN (1) CN116897201A (enExample)
AU (1) AU2022226163A1 (enExample)
CA (1) CA3206049A1 (enExample)
WO (1) WO2022182763A1 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024243552A1 (en) * 2023-05-25 2024-11-28 Board Of Regents, The University Of Texas System Methods using st2 to identify patients for cancer therapy
WO2025096685A1 (en) * 2023-10-30 2025-05-08 City Of Hope Methods of making and using regulatory t cells for treatment of autoimmune disorders and cancers

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007538000A (ja) * 2004-01-08 2007-12-27 リージエンツ・オブ・ザ・ユニバーシテイ・オブ・カリフオルニア 制御性ｔ細胞は自己免疫を抑制する
WO2011126806A1 (en) * 2010-04-08 2011-10-13 The Trustees Of The University Of Pennsylvania Methods to expand a t regulatory cell master cell bank
US9018006B2 (en) * 2010-07-23 2015-04-28 The University Of Toledo Stable Tregs and related materials and methods
WO2013131045A1 (en) * 2012-03-02 2013-09-06 The Regents Of The University Of California Expansion of alloantigen-reactive regulatory t cells
WO2014183056A1 (en) * 2013-05-10 2014-11-13 The Henry M. Jackson Foundation For The Advancement Of Military Medicine, Inc. Design and use of specific regulatory t-cells to induce immune tolerance
EP3551749B1 (en) * 2016-12-07 2023-09-27 East Carolina University Compositions and methods for in vitro cultivation and/or expansion of regulatory t cells
EP3571294A1 (en) * 2017-01-18 2019-11-27 F1 Oncology, Inc. Methods of transducing and expanding immune cells and uses thereof
CN110678190A (zh) * 2017-03-28 2020-01-10 宾夕法尼亚大学董事会 保护移植的组织免受排斥的方法
US10842815B2 (en) * 2017-06-16 2020-11-24 Stemspine Llc Perispinal perfusion by administration of T regulatory cells alone or in combination with angiogenic cell therapies
EP3716988A4 (en) * 2017-11-29 2021-12-15 Figene, LLC INTERACTION OF FIBROBLASTS AND IMMUNE CELLS FOR THE ACTIVATION AND USE THEREOF
JP2021524233A (ja) * 2018-05-17 2021-09-13 リージェンツ オブ ザ ユニバーシティ オブ ミネソタ 薬物耐性免疫細胞及びその用途
JP7642548B2 (ja) * 2019-02-01 2025-03-10 ケーエスキュー セラピューティクス， インコーポレイテッド 免疫療法の改善のための遺伝子調節組成物及び遺伝子調節方法

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Publication number Publication date
EP4297757A4 (en) 2025-02-26
US20240228962A9 (en) 2024-07-11
AU2022226163A1 (en) 2023-09-28
EP4297757A1 (en) 2024-01-03
AU2022226163A9 (en) 2024-01-18
US20240132843A1 (en) 2024-04-25
WO2022182763A1 (en) 2022-09-01
CN116897201A (zh) 2023-10-17
JP2024507373A (ja) 2024-02-19

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