CA3196715A1 - Engineered phosphopentomutase variant enzymes - Google Patents

Engineered phosphopentomutase variant enzymes

Info

Publication number
CA3196715A1
CA3196715A1 CA3196715A CA3196715A CA3196715A1 CA 3196715 A1 CA3196715 A1 CA 3196715A1 CA 3196715 A CA3196715 A CA 3196715A CA 3196715 A CA3196715 A CA 3196715A CA 3196715 A1 CA3196715 A1 CA 3196715A1
Authority
CA
Canada
Prior art keywords
seq
sequence
phosphopentomutase
engineered
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3196715A
Other languages
English (en)
French (fr)
Inventor
Jonathan VROOM
Santhosh Sivaramakrishnan
Jessica Anna HURTAK
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Codexis Inc
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of CA3196715A1 publication Critical patent/CA3196715A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/02—Phosphotransferases (phosphomutases) (5.4.2)
    • C12Y504/02007—Phosphopentomutase (5.4.2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA3196715A 2020-10-07 2021-10-05 Engineered phosphopentomutase variant enzymes Pending CA3196715A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063088556P 2020-10-07 2020-10-07
US63/088,556 2020-10-07
PCT/US2021/053626 WO2022076454A1 (en) 2020-10-07 2021-10-05 Engineered phosphopentomutase variant enzymes

Publications (1)

Publication Number Publication Date
CA3196715A1 true CA3196715A1 (en) 2022-04-14

Family

ID=81125465

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3196715A Pending CA3196715A1 (en) 2020-10-07 2021-10-05 Engineered phosphopentomutase variant enzymes

Country Status (8)

Country Link
US (1) US12668789B2 (enExample)
EP (1) EP4225909A4 (enExample)
JP (1) JP2023544783A (enExample)
CN (1) CN116710558A (enExample)
AU (1) AU2021358938A1 (enExample)
CA (1) CA3196715A1 (enExample)
IL (1) IL301857A (enExample)
WO (1) WO2022076454A1 (enExample)

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WO1998031816A1 (en) 1997-01-17 1998-07-23 Regents Of The University Of Minnesota Dna molecules and protein displaying improved triazine compound degrading ability
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JP2002517995A (ja) 1998-06-17 2002-06-25 マキシジェン， インコーポレイテッド 所望の特性を有するポリヌクレオチドを生成するための方法
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US8457903B1 (en) 1999-01-19 2013-06-04 Codexis Mayflower Holdings, Llc Method and/or apparatus for determining codons
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Also Published As

Publication number Publication date
EP4225909A4 (en) 2025-02-12
US12668789B2 (en) 2026-06-30
EP4225909A1 (en) 2023-08-16
US20230374486A1 (en) 2023-11-23
IL301857A (en) 2023-06-01
CN116710558A (zh) 2023-09-05
AU2021358938A1 (en) 2023-04-27
WO2022076454A1 (en) 2022-04-14
JP2023544783A (ja) 2023-10-25

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