CA3163345A1 - Nucleic acid compositions - Google Patents

Nucleic acid compositions

Info

Publication number
CA3163345A1
CA3163345A1 CA3163345A CA3163345A CA3163345A1 CA 3163345 A1 CA3163345 A1 CA 3163345A1 CA 3163345 A CA3163345 A CA 3163345A CA 3163345 A CA3163345 A CA 3163345A CA 3163345 A1 CA3163345 A1 CA 3163345A1
Authority
CA
Canada
Prior art keywords
construct
expression cassette
dsrna
nucleotide sequence
promoter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3163345A
Other languages
English (en)
French (fr)
Inventor
James Robbins Abshire
Himanshu DHAMANKAR
William Farmer
Christopher Gregg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Greenlight Biosciences Inc
Original Assignee
Greenlight Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Greenlight Biosciences Inc filed Critical Greenlight Biosciences Inc
Publication of CA3163345A1 publication Critical patent/CA3163345A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C12N2330/51—Specially adapted vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/34—Vector systems having a special element relevant for transcription being a transcription initiation element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/36—Vector systems having a special element relevant for transcription being a transcription termination element

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
CA3163345A 2019-12-06 2020-12-04 Nucleic acid compositions Pending CA3163345A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962944824P 2019-12-06 2019-12-06
US62/944,824 2019-12-06
PCT/US2020/063490 WO2021113774A1 (en) 2019-12-06 2020-12-04 Nucleic acid compositions

Publications (1)

Publication Number Publication Date
CA3163345A1 true CA3163345A1 (en) 2021-06-10

Family

ID=74046190

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3163345A Pending CA3163345A1 (en) 2019-12-06 2020-12-04 Nucleic acid compositions

Country Status (10)

Country Link
US (1) US20220411792A1 (https=)
EP (1) EP4069843A1 (https=)
JP (1) JP2023505188A (https=)
KR (1) KR20220113437A (https=)
CN (1) CN115397982A (https=)
AU (1) AU2020395244A1 (https=)
CA (1) CA3163345A1 (https=)
CL (1) CL2022001481A1 (https=)
MX (1) MX2022006748A (https=)
WO (1) WO2021113774A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12077763B2 (en) 2021-03-08 2024-09-03 Greenlight Biosciences, Inc. RNA-based control of powdery mildew
MX2023012833A (es) * 2021-04-30 2024-01-23 Greenlight Biosciences Inc Opciones terapéuticas y composiciones de arn mensajero.
TW202306573A (zh) * 2021-05-26 2023-02-16 比利時商ｅＴｈｅＲＮＡ免疫治療公司 減少雙股ｒｎａ副產物形成的方法
CN121889507A (zh) * 2023-08-29 2026-04-17 南京金斯瑞生物科技有限公司 用于非b型dna序列的克隆/表达载体

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2822164B1 (fr) * 2001-03-19 2004-06-18 Centre Nat Rech Scient Polypeptides derives des arn polymerases, et leurs utilisations
WO2005040388A2 (en) * 2003-08-22 2005-05-06 Nucleonics Inc. Eukariotic expression systems for expression of inhibitory rna in multiple intracellular compartments
RU2420584C1 (ru) * 2009-10-29 2011-06-10 Государственное образовательное учреждение высшего профессионального образования "Российский государственный медицинский университет Федерального агентства по здравоохранению и социальному развитию" (ГОУ ВПО РГМУ Росздрава) Лентивирусная репортерная векторная система для измерения активности ключевых транскрипционных факторов, отражающих функциональное состояние сигнальных путей клетки
US9254311B2 (en) * 2012-04-02 2016-02-09 Moderna Therapeutics, Inc. Modified polynucleotides for the production of proteins
BR112020007068A2 (pt) 2017-10-11 2020-10-06 Greenlight Biosciences, Inc. métodos e composições para produção de nucleosídeo trifosfato e ácido ribonucleico
AU2020253354A1 (en) * 2019-03-29 2021-10-28 Greenlight Biosciences, Inc. Cell-free production of ribonucleic acid

Also Published As

Publication number Publication date
CL2022001481A1 (es) 2023-03-17
EP4069843A1 (en) 2022-10-12
MX2022006748A (es) 2023-01-19
WO2021113774A1 (en) 2021-06-10
AU2020395244A1 (en) 2022-06-16
US20220411792A1 (en) 2022-12-29
KR20220113437A (ko) 2022-08-12
JP2023505188A (ja) 2023-02-08
CN115397982A (zh) 2022-11-25

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