CN115397982A - 核酸组合物 - Google Patents

核酸组合物 Download PDF

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Publication number
CN115397982A
CN115397982A CN202080090794.9A CN202080090794A CN115397982A CN 115397982 A CN115397982 A CN 115397982A CN 202080090794 A CN202080090794 A CN 202080090794A CN 115397982 A CN115397982 A CN 115397982A
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China
Prior art keywords
expression cassette
construct
dsrna
promoter
dna
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Pending
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CN202080090794.9A
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English (en)
Chinese (zh)
Inventor
詹姆斯·罗宾斯·阿布希尔
希曼舒·达曼卡尔
威廉·法默
克里斯多夫·格雷格
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Greenlight Biosciences Inc
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Greenlight Biosciences Inc
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Application filed by Greenlight Biosciences Inc filed Critical Greenlight Biosciences Inc
Publication of CN115397982A publication Critical patent/CN115397982A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C12N2330/51—Specially adapted vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/34—Vector systems having a special element relevant for transcription being a transcription initiation element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/36—Vector systems having a special element relevant for transcription being a transcription termination element

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
CN202080090794.9A 2019-12-06 2020-12-04 核酸组合物 Pending CN115397982A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962944824P 2019-12-06 2019-12-06
US62/944,824 2019-12-06
PCT/US2020/063490 WO2021113774A1 (en) 2019-12-06 2020-12-04 Nucleic acid compositions

Publications (1)

Publication Number Publication Date
CN115397982A true CN115397982A (zh) 2022-11-25

Family

ID=74046190

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080090794.9A Pending CN115397982A (zh) 2019-12-06 2020-12-04 核酸组合物

Country Status (10)

Country Link
US (1) US20220411792A1 (https=)
EP (1) EP4069843A1 (https=)
JP (1) JP2023505188A (https=)
KR (1) KR20220113437A (https=)
CN (1) CN115397982A (https=)
AU (1) AU2020395244A1 (https=)
CA (1) CA3163345A1 (https=)
CL (1) CL2022001481A1 (https=)
MX (1) MX2022006748A (https=)
WO (1) WO2021113774A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025045133A1 (zh) * 2023-08-29 2025-03-06 南京金斯瑞生物科技有限公司 用于非b型dna序列的克隆/表达载体

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12077763B2 (en) 2021-03-08 2024-09-03 Greenlight Biosciences, Inc. RNA-based control of powdery mildew
MX2023012833A (es) * 2021-04-30 2024-01-23 Greenlight Biosciences Inc Opciones terapéuticas y composiciones de arn mensajero.
TW202306573A (zh) * 2021-05-26 2023-02-16 比利時商ｅＴｈｅＲＮＡ免疫治療公司 減少雙股ｒｎａ副產物形成的方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2822164B1 (fr) * 2001-03-19 2004-06-18 Centre Nat Rech Scient Polypeptides derives des arn polymerases, et leurs utilisations
WO2005040388A2 (en) * 2003-08-22 2005-05-06 Nucleonics Inc. Eukariotic expression systems for expression of inhibitory rna in multiple intracellular compartments
RU2420584C1 (ru) * 2009-10-29 2011-06-10 Государственное образовательное учреждение высшего профессионального образования "Российский государственный медицинский университет Федерального агентства по здравоохранению и социальному развитию" (ГОУ ВПО РГМУ Росздрава) Лентивирусная репортерная векторная система для измерения активности ключевых транскрипционных факторов, отражающих функциональное состояние сигнальных путей клетки
US9254311B2 (en) * 2012-04-02 2016-02-09 Moderna Therapeutics, Inc. Modified polynucleotides for the production of proteins
BR112020007068A2 (pt) 2017-10-11 2020-10-06 Greenlight Biosciences, Inc. métodos e composições para produção de nucleosídeo trifosfato e ácido ribonucleico
AU2020253354A1 (en) * 2019-03-29 2021-10-28 Greenlight Biosciences, Inc. Cell-free production of ribonucleic acid

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025045133A1 (zh) * 2023-08-29 2025-03-06 南京金斯瑞生物科技有限公司 用于非b型dna序列的克隆/表达载体

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Publication number Publication date
CL2022001481A1 (es) 2023-03-17
EP4069843A1 (en) 2022-10-12
CA3163345A1 (en) 2021-06-10
MX2022006748A (es) 2023-01-19
WO2021113774A1 (en) 2021-06-10
AU2020395244A1 (en) 2022-06-16
US20220411792A1 (en) 2022-12-29
KR20220113437A (ko) 2022-08-12
JP2023505188A (ja) 2023-02-08

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