CA3114606A1 - Simultaneous, sequencing-based analysis of proteins, nucleosomes, and cell-free nucleic acids from a single biological sample - Google Patents

Simultaneous, sequencing-based analysis of proteins, nucleosomes, and cell-free nucleic acids from a single biological sample Download PDF

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Publication number
CA3114606A1
CA3114606A1 CA3114606A CA3114606A CA3114606A1 CA 3114606 A1 CA3114606 A1 CA 3114606A1 CA 3114606 A CA3114606 A CA 3114606A CA 3114606 A CA3114606 A CA 3114606A CA 3114606 A1 CA3114606 A1 CA 3114606A1
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CA
Canada
Prior art keywords
dna
sequence
nucleic acid
adapter
sample
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3114606A
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English (en)
French (fr)
Inventor
Patrick A. Arensdorf
Damek SPACEK
Christopher E. ELLISON
Samuel Levy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ClearNote Health Inc
Original Assignee
Bluestar Genomics Inc
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Filing date
Publication date
Application filed by Bluestar Genomics Inc filed Critical Bluestar Genomics Inc
Publication of CA3114606A1 publication Critical patent/CA3114606A1/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3114606A 2018-10-04 2019-10-03 Simultaneous, sequencing-based analysis of proteins, nucleosomes, and cell-free nucleic acids from a single biological sample Pending CA3114606A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862741473P 2018-10-04 2018-10-04
US62/741,473 2018-10-04
PCT/US2019/054582 WO2020072829A2 (en) 2018-10-04 2019-10-03 Simultaneous, sequencing-based analysis of proteins, nucleosomes, and cell-free nucleic acids from a single biological sample

Publications (1)

Publication Number Publication Date
CA3114606A1 true CA3114606A1 (en) 2020-04-09

Family

ID=68296828

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3114606A Pending CA3114606A1 (en) 2018-10-04 2019-10-03 Simultaneous, sequencing-based analysis of proteins, nucleosomes, and cell-free nucleic acids from a single biological sample

Country Status (9)

Country Link
US (1) US20210380971A1 (cg-RX-API-DMAC7.html)
EP (1) EP3861132A2 (cg-RX-API-DMAC7.html)
JP (1) JP2022504078A (cg-RX-API-DMAC7.html)
CN (1) CN113166796A (cg-RX-API-DMAC7.html)
AU (1) AU2019354789A1 (cg-RX-API-DMAC7.html)
CA (1) CA3114606A1 (cg-RX-API-DMAC7.html)
MX (1) MX2021003847A (cg-RX-API-DMAC7.html)
SG (1) SG11202102954QA (cg-RX-API-DMAC7.html)
WO (1) WO2020072829A2 (cg-RX-API-DMAC7.html)

Cited By (1)

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Publication number Priority date Publication date Assignee Title
CN116403645A (zh) * 2023-03-03 2023-07-07 阿里巴巴(中国)有限公司 转录因子结合位点的预测方法及装置

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CN113517026B (zh) * 2021-06-16 2022-08-19 苏州拉索生物芯片科技有限公司 应用于生物制品的标签序列的生成方法、系统、智能终端及计算机可读存储介质
WO2023288222A1 (en) * 2021-07-12 2023-01-19 The Trustees Of The University Of Pennsylvania Modified adapters for enzymatic dna deamination and methods of use thereof for epigenetic sequencing of free and immobilized dna
WO2023287876A1 (en) * 2021-07-15 2023-01-19 University Of Washington Efficient duplex sequencing using high fidelity next generation sequencing reads
EP4405496A4 (en) * 2021-09-23 2025-01-01 Genomic Testing Cooperative, LCA COMPOSITIONS AND METHODS FOR TARGETED NGS SEQUENCING OF CFRNA AND CFTNA
US20230086611A1 (en) * 2021-09-23 2023-03-23 Genomic Testing Cooperative, LCA Compositions and Methods for Targeted NGS Sequencing of cfRNA and cfTNA
WO2023097295A1 (en) * 2021-11-24 2023-06-01 Alida Biosciences, Inc. Rna and dna analysis using engineered surfaces
CN114934110A (zh) * 2022-06-15 2022-08-23 江西烈冰生物科技有限公司 用于获取基因表达的原始位置的生物芯片、试剂盒及方法
EP4623079A1 (en) * 2022-11-23 2025-10-01 Alida Biosciences, Inc. Chromatin profiling compositions and methods

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US20090093378A1 (en) 2007-08-29 2009-04-09 Helen Bignell Method for sequencing a polynucleotide template
WO2010037001A2 (en) 2008-09-26 2010-04-01 Immune Disease Institute, Inc. Selective oxidation of 5-methylcytosine by tet-family proteins
US20100323348A1 (en) 2009-01-31 2010-12-23 The Regents Of The University Of Colorado, A Body Corporate Methods and Compositions for Using Error-Detecting and/or Error-Correcting Barcodes in Nucleic Acid Amplification Process
EP2816111B1 (en) 2009-08-14 2016-04-13 Epicentre Technologies Corporation Methods, compositions, and kits for generating rRNA-depleted samples or isolating rRNA from samples
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WO2017075265A1 (en) * 2015-10-28 2017-05-04 The Broad Institute, Inc. Multiplex analysis of single cell constituents
AU2017246318B2 (en) 2016-04-07 2023-07-27 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnostics by sequencing 5-hydroxymethylated cell-free DNA
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116403645A (zh) * 2023-03-03 2023-07-07 阿里巴巴(中国)有限公司 转录因子结合位点的预测方法及装置
CN116403645B (zh) * 2023-03-03 2024-01-09 阿里巴巴(中国)有限公司 转录因子结合位点的预测方法及装置

Also Published As

Publication number Publication date
EP3861132A2 (en) 2021-08-11
US20210380971A1 (en) 2021-12-09
JP2022504078A (ja) 2022-01-13
CN113166796A (zh) 2021-07-23
AU2019354789A1 (en) 2021-05-27
WO2020072829A3 (en) 2020-08-13
WO2020072829A2 (en) 2020-04-09
MX2021003847A (es) 2021-05-27
SG11202102954QA (en) 2021-04-29

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