CA3053009A1 - Optics, device, and system for assaying - Google Patents
Optics, device, and system for assayingInfo
- Publication number
- CA3053009A1 CA3053009A1 CA3053009A CA3053009A CA3053009A1 CA 3053009 A1 CA3053009 A1 CA 3053009A1 CA 3053009 A CA3053009 A CA 3053009A CA 3053009 A CA3053009 A CA 3053009A CA 3053009 A1 CA3053009 A1 CA 3053009A1
- Authority
- CA
- Canada
- Prior art keywords
- sample
- optical
- optical assembly
- light
- imaging
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000003384 imaging method Methods 0.000 claims abstract description 255
- 230000003287 optical effect Effects 0.000 claims description 445
- 238000005286 illumination Methods 0.000 claims description 144
- 125000006850 spacer group Chemical group 0.000 claims description 118
- 239000007788 liquid Substances 0.000 claims description 76
- 239000000835 fiber Substances 0.000 claims description 32
- 239000013307 optical fiber Substances 0.000 claims description 31
- 230000005284 excitation Effects 0.000 claims description 26
- 239000000758 substrate Substances 0.000 claims description 21
- 239000006096 absorbing agent Substances 0.000 claims description 20
- 238000012545 processing Methods 0.000 claims description 19
- 230000037361 pathway Effects 0.000 claims description 16
- 230000005540 biological transmission Effects 0.000 claims description 6
- 238000010521 absorption reaction Methods 0.000 claims description 4
- 238000000799 fluorescence microscopy Methods 0.000 claims description 4
- 230000004044 response Effects 0.000 claims description 4
- 230000000903 blocking effect Effects 0.000 claims description 2
- 230000001419 dependent effect Effects 0.000 claims description 2
- 230000010287 polarization Effects 0.000 claims description 2
- 239000010409 thin film Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 141
- 239000000523 sample Substances 0.000 description 560
- 238000010186 staining Methods 0.000 description 76
- 239000003795 chemical substances by application Substances 0.000 description 61
- 238000001514 detection method Methods 0.000 description 60
- 239000012491 analyte Substances 0.000 description 58
- 210000001519 tissue Anatomy 0.000 description 57
- 238000004422 calculation algorithm Methods 0.000 description 38
- 230000008859 change Effects 0.000 description 30
- 238000003825 pressing Methods 0.000 description 30
- 230000001105 regulatory effect Effects 0.000 description 29
- 238000000151 deposition Methods 0.000 description 26
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 24
- 210000004027 cell Anatomy 0.000 description 23
- 230000008569 process Effects 0.000 description 23
- -1 reservoirs Substances 0.000 description 22
- 238000012360 testing method Methods 0.000 description 22
- 238000012549 training Methods 0.000 description 21
- 238000010295 mobile communication Methods 0.000 description 20
- 238000003860 storage Methods 0.000 description 18
- 230000009977 dual effect Effects 0.000 description 17
- 239000000427 antigen Substances 0.000 description 16
- 102000036639 antigens Human genes 0.000 description 16
- 108091007433 antigens Proteins 0.000 description 16
- 239000000243 solution Substances 0.000 description 15
- 238000004458 analytical method Methods 0.000 description 14
- 210000004369 blood Anatomy 0.000 description 14
- 239000008280 blood Substances 0.000 description 14
- 238000013461 design Methods 0.000 description 13
- 238000007901 in situ hybridization Methods 0.000 description 13
- 238000003325 tomography Methods 0.000 description 13
- 239000002981 blocking agent Substances 0.000 description 12
- 238000012546 transfer Methods 0.000 description 11
- 101000863856 Homo sapiens Shiftless antiviral inhibitor of ribosomal frameshifting protein Proteins 0.000 description 10
- 230000006870 function Effects 0.000 description 10
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 9
- 239000003153 chemical reaction reagent Substances 0.000 description 9
- 230000008021 deposition Effects 0.000 description 9
- 230000004807 localization Effects 0.000 description 9
- 229960000907 methylthioninium chloride Drugs 0.000 description 9
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 8
- 238000003556 assay Methods 0.000 description 8
- 238000007398 colorimetric assay Methods 0.000 description 8
- 238000013527 convolutional neural network Methods 0.000 description 8
- 238000000386 microscopy Methods 0.000 description 8
- 230000009466 transformation Effects 0.000 description 8
- 238000004737 colorimetric analysis Methods 0.000 description 7
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 7
- 239000003550 marker Substances 0.000 description 7
- 230000008901 benefit Effects 0.000 description 6
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 6
- 210000002939 cerumen Anatomy 0.000 description 6
- 238000003745 diagnosis Methods 0.000 description 6
- 238000009826 distribution Methods 0.000 description 6
- 230000033001 locomotion Effects 0.000 description 6
- 238000010801 machine learning Methods 0.000 description 6
- 239000012188 paraffin wax Substances 0.000 description 6
- 239000008191 permeabilizing agent Substances 0.000 description 6
- 241000894007 species Species 0.000 description 6
- NALREUIWICQLPS-UHFFFAOYSA-N 7-imino-n,n-dimethylphenothiazin-3-amine;hydrochloride Chemical compound [Cl-].C1=C(N)C=C2SC3=CC(=[N+](C)C)C=CC3=NC2=C1 NALREUIWICQLPS-UHFFFAOYSA-N 0.000 description 5
- 108020004414 DNA Proteins 0.000 description 5
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 5
- 239000000975 dye Substances 0.000 description 5
- ZBQZBWKNGDEDOA-UHFFFAOYSA-N eosin B Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC([N+]([O-])=O)=C(O)C(Br)=C1OC1=C2C=C([N+]([O-])=O)C(O)=C1Br ZBQZBWKNGDEDOA-UHFFFAOYSA-N 0.000 description 5
- SEACYXSIPDVVMV-UHFFFAOYSA-L eosin Y Chemical compound [Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C([O-])=C(Br)C=C21 SEACYXSIPDVVMV-UHFFFAOYSA-L 0.000 description 5
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 5
- 230000002055 immunohistochemical effect Effects 0.000 description 5
- 230000031700 light absorption Effects 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 230000007246 mechanism Effects 0.000 description 5
- 238000012544 monitoring process Methods 0.000 description 5
- 239000002751 oligonucleotide probe Substances 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- OCKKUZVCJCWWHM-UHFFFAOYSA-L (7-amino-8-methylphenoxazin-3-ylidene)-diethylazanium;dichlorozinc;dichloride Chemical compound [Cl-].[Cl-].[Cl-].[Cl-].[Zn+2].CC1=C(N)C=C2OC3=CC(=[N+](CC)CC)C=CC3=NC2=C1.CC1=C(N)C=C2OC3=CC(=[N+](CC)CC)C=CC3=NC2=C1 OCKKUZVCJCWWHM-UHFFFAOYSA-L 0.000 description 4
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 4
- JKYKXTRKURYNGW-UHFFFAOYSA-N 3,4-dihydroxy-9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid Chemical compound O=C1C2=CC=CC=C2C(=O)C2=C1C(O)=C(O)C(S(O)(=O)=O)=C2 JKYKXTRKURYNGW-UHFFFAOYSA-N 0.000 description 4
- AXDJCCTWPBKUKL-UHFFFAOYSA-N 4-[(4-aminophenyl)-(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]aniline;hydron;chloride Chemical compound Cl.C1=CC(=N)C(C)=CC1=C(C=1C=CC(N)=CC=1)C1=CC=C(N)C=C1 AXDJCCTWPBKUKL-UHFFFAOYSA-N 0.000 description 4
- ALJHHTHBYJROOG-UHFFFAOYSA-N 7-(dimethylamino)phenothiazin-3-one Chemical compound C1=CC(=O)C=C2SC3=CC(N(C)C)=CC=C3N=C21 ALJHHTHBYJROOG-UHFFFAOYSA-N 0.000 description 4
- MPVDXIMFBOLMNW-ISLYRVAYSA-N 7-hydroxy-8-[(E)-phenyldiazenyl]naphthalene-1,3-disulfonic acid Chemical compound OC1=CC=C2C=C(S(O)(=O)=O)C=C(S(O)(=O)=O)C2=C1\N=N\C1=CC=CC=C1 MPVDXIMFBOLMNW-ISLYRVAYSA-N 0.000 description 4
- CKLBXIYTBHXJEH-UHFFFAOYSA-J 75881-23-1 Chemical compound [Cl-].[Cl-].[Cl-].[Cl-].[Cu+2].[N-]1C(N=C2C3=CC=C(CSC(N(C)C)=[N+](C)C)C=C3C(N=C3C4=CC=C(CSC(N(C)C)=[N+](C)C)C=C4C(=N4)[N-]3)=N2)=C(C=C(CSC(N(C)C)=[N+](C)C)C=C2)C2=C1N=C1C2=CC(CSC(N(C)C)=[N+](C)C)=CC=C2C4=N1 CKLBXIYTBHXJEH-UHFFFAOYSA-J 0.000 description 4
- QFIIYGZAUXVPSZ-UHFFFAOYSA-N 8-(2,4-dihydroxy-6-methylanilino)-2-(2,4-dihydroxy-6-methylphenyl)imino-7-hydroxy-1,9-dimethyldibenzofuran-3-one Chemical compound CC1=CC(=CC(=C1NC2=C(C3=C(C=C2O)OC4=CC(=O)C(=NC5=C(C=C(C=C5C)O)O)C(=C43)C)C)O)O QFIIYGZAUXVPSZ-UHFFFAOYSA-N 0.000 description 4
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 description 4
- XXACTDWGHQXLGW-UHFFFAOYSA-M Janus Green B chloride Chemical compound [Cl-].C12=CC(N(CC)CC)=CC=C2N=C2C=CC(\N=N\C=3C=CC(=CC=3)N(C)C)=CC2=[N+]1C1=CC=CC=C1 XXACTDWGHQXLGW-UHFFFAOYSA-M 0.000 description 4
- WWKGVZASJYXZKN-UHFFFAOYSA-N Methyl violet 2B Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC(N)=CC=1)=C1C=CC(=[N+](C)C)C=C1 WWKGVZASJYXZKN-UHFFFAOYSA-N 0.000 description 4
- 239000004218 Orcein Substances 0.000 description 4
- PLXBWHJQWKZRKG-UHFFFAOYSA-N Resazurin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3[N+]([O-])=C21 PLXBWHJQWKZRKG-UHFFFAOYSA-N 0.000 description 4
- FHNINJWBTRXEBC-UHFFFAOYSA-N Sudan III Chemical compound OC1=CC=C2C=CC=CC2=C1N=NC(C=C1)=CC=C1N=NC1=CC=CC=C1 FHNINJWBTRXEBC-UHFFFAOYSA-N 0.000 description 4
- AXIKDPDWFVPGOD-UHFFFAOYSA-O [7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium;2-(2,4,5,7-tetrabromo-3,6-dihydroxyxanthen-10-ium-9-yl)benzoic acid Chemical compound C1=CC(=[N+](C)C)C=C2SC3=CC(N(C)C)=CC=C3N=C21.OC(=O)C1=CC=CC=C1C1=C(C=C(Br)C(O)=C2Br)C2=[O+]C2=C1C=C(Br)C(O)=C2Br AXIKDPDWFVPGOD-UHFFFAOYSA-O 0.000 description 4
- ZHAFUINZIZIXFC-UHFFFAOYSA-N [9-(dimethylamino)-10-methylbenzo[a]phenoxazin-5-ylidene]azanium;chloride Chemical compound [Cl-].O1C2=CC(=[NH2+])C3=CC=CC=C3C2=NC2=C1C=C(N(C)C)C(C)=C2 ZHAFUINZIZIXFC-UHFFFAOYSA-N 0.000 description 4
- RZUBARUFLYGOGC-MTHOTQAESA-L acid fuchsin Chemical compound [Na+].[Na+].[O-]S(=O)(=O)C1=C(N)C(C)=CC(C(=C\2C=C(C(=[NH2+])C=C/2)S([O-])(=O)=O)\C=2C=C(C(N)=CC=2)S([O-])(=O)=O)=C1 RZUBARUFLYGOGC-MTHOTQAESA-L 0.000 description 4
- DGOBMKYRQHEFGQ-UHFFFAOYSA-L acid green 5 Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 DGOBMKYRQHEFGQ-UHFFFAOYSA-L 0.000 description 4
- CQPFMGBJSMSXLP-UHFFFAOYSA-M acid orange 7 Chemical compound [Na+].OC1=CC=C2C=CC=CC2=C1N=NC1=CC=C(S([O-])(=O)=O)C=C1 CQPFMGBJSMSXLP-UHFFFAOYSA-M 0.000 description 4
- JPIYZTWMUGTEHX-UHFFFAOYSA-N auramine O free base Chemical compound C1=CC(N(C)C)=CC=C1C(=N)C1=CC=C(N(C)C)C=C1 JPIYZTWMUGTEHX-UHFFFAOYSA-N 0.000 description 4
- QZKHGYGBYOUFGK-UHFFFAOYSA-L azocarmine B Chemical compound [Na+].[Na+].[O-]S(=O)(=O)C1=CC(S(=O)(=O)[O-])=CC=C1NC(C1=CC(=CC=C1C1=NC2=CC=CC=C22)S([O-])(=O)=O)=CC1=[N+]2C1=CC=CC=C1 QZKHGYGBYOUFGK-UHFFFAOYSA-L 0.000 description 4
- LUERODMRBLNCFK-UHFFFAOYSA-M azocarmine G Chemical compound [Na+].C1=CC(S(=O)(=O)[O-])=CC=C1NC(C1=CC(=CC=C1C1=NC2=CC=CC=C22)S([O-])(=O)=O)=CC1=[N+]2C1=CC=CC=C1 LUERODMRBLNCFK-UHFFFAOYSA-M 0.000 description 4
- BDFZFGDTHFGWRQ-UHFFFAOYSA-N basic brown 1 Chemical compound NC1=CC(N)=CC=C1N=NC1=CC=CC(N=NC=2C(=CC(N)=CC=2)N)=C1 BDFZFGDTHFGWRQ-UHFFFAOYSA-N 0.000 description 4
- 210000001124 body fluid Anatomy 0.000 description 4
- 238000000339 bright-field microscopy Methods 0.000 description 4
- 229960001506 brilliant green Drugs 0.000 description 4
- 235000012730 carminic acid Nutrition 0.000 description 4
- XRPLBRIHZGVJIC-UHFFFAOYSA-L chembl3182776 Chemical compound [Na+].[Na+].NC1=CC(N)=CC=C1N=NC1=CC=C(C=2C=CC(=CC=2)N=NC=2C(=CC3=CC(=C(N=NC=4C=CC=CC=4)C(O)=C3C=2N)S([O-])(=O)=O)S([O-])(=O)=O)C=C1 XRPLBRIHZGVJIC-UHFFFAOYSA-L 0.000 description 4
- IQFVPQOLBLOTPF-HKXUKFGYSA-L congo red Chemical compound [Na+].[Na+].C1=CC=CC2=C(N)C(/N=N/C3=CC=C(C=C3)C3=CC=C(C=C3)/N=N/C3=C(C4=CC=CC=C4C(=C3)S([O-])(=O)=O)N)=CC(S([O-])(=O)=O)=C21 IQFVPQOLBLOTPF-HKXUKFGYSA-L 0.000 description 4
- 239000013078 crystal Substances 0.000 description 4
- OOYIOIOOWUGAHD-UHFFFAOYSA-L disodium;2',4',5',7'-tetrabromo-4,5,6,7-tetrachloro-3-oxospiro[2-benzofuran-1,9'-xanthene]-3',6'-diolate Chemical compound [Na+].[Na+].O1C(=O)C(C(=C(Cl)C(Cl)=C2Cl)Cl)=C2C21C1=CC(Br)=C([O-])C(Br)=C1OC1=C(Br)C([O-])=C(Br)C=C21 OOYIOIOOWUGAHD-UHFFFAOYSA-L 0.000 description 4
- IINNWAYUJNWZRM-UHFFFAOYSA-L erythrosin B Chemical compound [Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=C(I)C(=O)C(I)=C2OC2=C(I)C([O-])=C(I)C=C21 IINNWAYUJNWZRM-UHFFFAOYSA-L 0.000 description 4
- UKZQEOHHLOYJLY-UHFFFAOYSA-M ethyl eosin Chemical compound [K+].CCOC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C([O-])=C(Br)C=C21 UKZQEOHHLOYJLY-UHFFFAOYSA-M 0.000 description 4
- IDAQSADEMXDTKN-UHFFFAOYSA-L ethyl green Chemical compound [Cl-].[Br-].C1=CC([N+](C)(C)CC)=CC=C1C(C=1C=CC(=CC=1)N(C)C)=C1C=CC(=[N+](C)C)C=C1 IDAQSADEMXDTKN-UHFFFAOYSA-L 0.000 description 4
- MNEJSIQJOSRAAA-UHFFFAOYSA-O ethyl-[4-[[4-[ethyl-[(3-sulfophenyl)methyl]amino]phenyl]-(4-hydroxy-3-sulfophenyl)methylidene]cyclohexa-2,5-dien-1-ylidene]-[(3-sulfophenyl)methyl]azanium Chemical compound C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S(O)(=O)=O)C=2C=C(C(O)=CC=2)S(O)(=O)=O)C=CC=1N(CC)CC1=CC=CC(S(O)(=O)=O)=C1 MNEJSIQJOSRAAA-UHFFFAOYSA-O 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- KHLVKKOJDHCJMG-QDBORUFSSA-L indigo carmine Chemical compound [Na+].[Na+].N/1C2=CC=C(S([O-])(=O)=O)C=C2C(=O)C\1=C1/NC2=CC=C(S(=O)(=O)[O-])C=C2C1=O KHLVKKOJDHCJMG-QDBORUFSSA-L 0.000 description 4
- 229960003988 indigo carmine Drugs 0.000 description 4
- 235000012738 indigotine Nutrition 0.000 description 4
- 239000004179 indigotine Substances 0.000 description 4
- 238000004020 luminiscence type Methods 0.000 description 4
- 229940107698 malachite green Drugs 0.000 description 4
- FDZZZRQASAIRJF-UHFFFAOYSA-M malachite green Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](C)C)C=C1 FDZZZRQASAIRJF-UHFFFAOYSA-M 0.000 description 4
- 229940012189 methyl orange Drugs 0.000 description 4
- STZCRXQWRGQSJD-GEEYTBSJSA-M methyl orange Chemical compound [Na+].C1=CC(N(C)C)=CC=C1\N=N\C1=CC=C(S([O-])(=O)=O)C=C1 STZCRXQWRGQSJD-GEEYTBSJSA-M 0.000 description 4
- 238000001000 micrograph Methods 0.000 description 4
- PGSADBUBUOPOJS-UHFFFAOYSA-N neutral red Chemical compound Cl.C1=C(C)C(N)=CC2=NC3=CC(N(C)C)=CC=C3N=C21 PGSADBUBUOPOJS-UHFFFAOYSA-N 0.000 description 4
- XJCPMUIIBDVFDM-UHFFFAOYSA-M nile blue A Chemical compound [Cl-].C1=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4[O+]=C3C=C(N)C2=C1 XJCPMUIIBDVFDM-UHFFFAOYSA-M 0.000 description 4
- 235000019248 orcein Nutrition 0.000 description 4
- AFAIELJLZYUNPW-UHFFFAOYSA-N pararosaniline free base Chemical compound C1=CC(N)=CC=C1C(C=1C=CC(N)=CC=1)=C1C=CC(=N)C=C1 AFAIELJLZYUNPW-UHFFFAOYSA-N 0.000 description 4
- NTGBUUXKGAZMSE-UHFFFAOYSA-N phenyl n-[4-[4-(4-methoxyphenyl)piperazin-1-yl]phenyl]carbamate Chemical compound C1=CC(OC)=CC=C1N1CCN(C=2C=CC(NC(=O)OC=3C=CC=CC=3)=CC=2)CC1 NTGBUUXKGAZMSE-UHFFFAOYSA-N 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- CXZRDVVUVDYSCQ-UHFFFAOYSA-M pyronin B Chemical compound [Cl-].C1=CC(=[N+](CC)CC)C=C2OC3=CC(N(CC)CC)=CC=C3C=C21 CXZRDVVUVDYSCQ-UHFFFAOYSA-M 0.000 description 4
- INCIMLINXXICKS-UHFFFAOYSA-M pyronin Y Chemical compound [Cl-].C1=CC(=[N+](C)C)C=C2OC3=CC(N(C)C)=CC=C3C=C21 INCIMLINXXICKS-UHFFFAOYSA-M 0.000 description 4
- 238000011002 quantification Methods 0.000 description 4
- 230000010076 replication Effects 0.000 description 4
- OARRHUQTFTUEOS-UHFFFAOYSA-N safranin Chemical compound [Cl-].C=12C=C(N)C(C)=CC2=NC2=CC(C)=C(N)C=C2[N+]=1C1=CC=CC=C1 OARRHUQTFTUEOS-UHFFFAOYSA-N 0.000 description 4
- 108700024661 strong silver Proteins 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- YCUVUDODLRLVIC-VPHDGDOJSA-N sudan black b Chemical compound C1=CC(=C23)NC(C)(C)NC2=CC=CC3=C1\N=N\C(C1=CC=CC=C11)=CC=C1\N=N\C1=CC=CC=C1 YCUVUDODLRLVIC-VPHDGDOJSA-N 0.000 description 4
- 229940099373 sudan iii Drugs 0.000 description 4
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 4
- 229950003937 tolonium Drugs 0.000 description 4
- HNONEKILPDHFOL-UHFFFAOYSA-M tolonium chloride Chemical compound [Cl-].C1=C(C)C(N)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 HNONEKILPDHFOL-UHFFFAOYSA-M 0.000 description 4
- XOSXWYQMOYSSKB-UHFFFAOYSA-L water blue Chemical compound [Na+].[Na+].[O-]S(=O)(=O)C1=C(N)C(C)=CC(C(=C2C=CC(C=C2)=NC=2C=CC(=CC=2)S([O-])(=O)=O)C=2C=CC(NC=3C=CC(=CC=3)S(O)(=O)=O)=CC=2)=C1 XOSXWYQMOYSSKB-UHFFFAOYSA-L 0.000 description 4
- 206010050337 Cerumen impaction Diseases 0.000 description 3
- 101000649946 Homo sapiens Vacuolar protein sorting-associated protein 29 Proteins 0.000 description 3
- 206010062717 Increased upper airway secretion Diseases 0.000 description 3
- 208000005228 Pericardial Effusion Diseases 0.000 description 3
- 206010036790 Productive cough Diseases 0.000 description 3
- 238000001069 Raman spectroscopy Methods 0.000 description 3
- 241000219061 Rheum Species 0.000 description 3
- 102100028290 Vacuolar protein sorting-associated protein 29 Human genes 0.000 description 3
- 239000000853 adhesive Substances 0.000 description 3
- 230000001070 adhesive effect Effects 0.000 description 3
- 210000004381 amniotic fluid Anatomy 0.000 description 3
- 210000003567 ascitic fluid Anatomy 0.000 description 3
- 239000012472 biological sample Substances 0.000 description 3
- 210000001268 chyle Anatomy 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 3
- 238000013135 deep learning Methods 0.000 description 3
- 238000009792 diffusion process Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005401 electroluminescence Methods 0.000 description 3
- 210000003060 endolymph Anatomy 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 210000003608 fece Anatomy 0.000 description 3
- 239000010408 film Substances 0.000 description 3
- 239000000834 fixative Substances 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 210000004211 gastric acid Anatomy 0.000 description 3
- 210000004051 gastric juice Anatomy 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 210000004251 human milk Anatomy 0.000 description 3
- 235000020256 human milk Nutrition 0.000 description 3
- 210000002751 lymph Anatomy 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 210000003097 mucus Anatomy 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 210000004912 pericardial fluid Anatomy 0.000 description 3
- 210000004049 perilymph Anatomy 0.000 description 3
- 208000026435 phlegm Diseases 0.000 description 3
- 238000005424 photoluminescence Methods 0.000 description 3
- 210000002381 plasma Anatomy 0.000 description 3
- 210000004910 pleural fluid Anatomy 0.000 description 3
- 210000004915 pus Anatomy 0.000 description 3
- 210000003296 saliva Anatomy 0.000 description 3
- 210000002374 sebum Anatomy 0.000 description 3
- 210000000582 semen Anatomy 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 210000003802 sputum Anatomy 0.000 description 3
- 208000024794 sputum Diseases 0.000 description 3
- 210000004243 sweat Anatomy 0.000 description 3
- 210000001179 synovial fluid Anatomy 0.000 description 3
- 210000001138 tear Anatomy 0.000 description 3
- 210000002700 urine Anatomy 0.000 description 3
- 210000004916 vomit Anatomy 0.000 description 3
- 230000008673 vomiting Effects 0.000 description 3
- WZUVPPKBWHMQCE-XJKSGUPXSA-N (+)-haematoxylin Chemical compound C12=CC(O)=C(O)C=C2C[C@]2(O)[C@H]1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-XJKSGUPXSA-N 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 2
- 102100028175 Abasic site processing protein HMCES Human genes 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 101001006387 Homo sapiens Abasic site processing protein HMCES Proteins 0.000 description 2
- 101100537375 Homo sapiens TMEM107 gene Proteins 0.000 description 2
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 102100036728 Transmembrane protein 107 Human genes 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 238000013528 artificial neural network Methods 0.000 description 2
- 239000000090 biomarker Substances 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 238000007405 data analysis Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 2
- 238000003364 immunohistochemistry Methods 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 210000002569 neuron Anatomy 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 239000002853 nucleic acid probe Substances 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 230000000737 periodic effect Effects 0.000 description 2
- 238000002310 reflectometry Methods 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000007447 staining method Methods 0.000 description 2
- 210000003813 thumb Anatomy 0.000 description 2
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 1
- QRXMUCSWCMTJGU-UHFFFAOYSA-N 5-bromo-4-chloro-3-indolyl phosphate Chemical compound C1=C(Br)C(Cl)=C2C(OP(O)(=O)O)=CNC2=C1 QRXMUCSWCMTJGU-UHFFFAOYSA-N 0.000 description 1
- 102100031650 C-X-C chemokine receptor type 4 Human genes 0.000 description 1
- 102100022210 COX assembly mitochondrial protein 2 homolog Human genes 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 108020004394 Complementary RNA Proteins 0.000 description 1
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 102100029203 F-box only protein 8 Human genes 0.000 description 1
- 101100321670 Fagopyrum esculentum FA18 gene Proteins 0.000 description 1
- 238000010867 Hoechst staining Methods 0.000 description 1
- 101000922348 Homo sapiens C-X-C chemokine receptor type 4 Proteins 0.000 description 1
- 101000900446 Homo sapiens COX assembly mitochondrial protein 2 homolog Proteins 0.000 description 1
- 101100280298 Homo sapiens FAM162A gene Proteins 0.000 description 1
- 101100334493 Homo sapiens FBXO8 gene Proteins 0.000 description 1
- 101100257194 Homo sapiens SMIM8 gene Proteins 0.000 description 1
- 101000741917 Homo sapiens Serine/threonine-protein phosphatase 1 regulatory subunit 10 Proteins 0.000 description 1
- 101000911790 Homo sapiens Sister chromatid cohesion protein DCC1 Proteins 0.000 description 1
- 101000631695 Homo sapiens Succinate dehydrogenase assembly factor 3, mitochondrial Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 1
- 101100170008 Oryza sativa subsp. japonica DBB1 gene Proteins 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 102100023788 Protein FAM162A Human genes 0.000 description 1
- 108020004518 RNA Probes Proteins 0.000 description 1
- 239000003391 RNA probe Substances 0.000 description 1
- 101100127688 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) FAA1 gene Proteins 0.000 description 1
- 101100127690 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) FAA2 gene Proteins 0.000 description 1
- 241000490025 Schefflera digitata Species 0.000 description 1
- 102100038743 Serine/threonine-protein phosphatase 1 regulatory subunit 10 Human genes 0.000 description 1
- 102100027040 Sister chromatid cohesion protein DCC1 Human genes 0.000 description 1
- 102100024789 Small integral membrane protein 8 Human genes 0.000 description 1
- 102100028996 Succinate dehydrogenase assembly factor 3, mitochondrial Human genes 0.000 description 1
- GYDJEQRTZSCIOI-UHFFFAOYSA-N Tranexamic acid Chemical compound NCC1CCC(C(O)=O)CC1 GYDJEQRTZSCIOI-UHFFFAOYSA-N 0.000 description 1
- 235000012544 Viola sororia Nutrition 0.000 description 1
- 241001106476 Violaceae Species 0.000 description 1
- 238000010817 Wright-Giemsa staining Methods 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 239000012148 binding buffer Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 238000009534 blood test Methods 0.000 description 1
- 239000011449 brick Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 239000003184 complementary RNA Substances 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 239000004567 concrete Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 229920005570 flexible polymer Polymers 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 238000002509 fluorescent in situ hybridization Methods 0.000 description 1
- 210000005224 forefinger Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 238000005534 hematocrit Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000011532 immunohistochemical staining Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000005305 interferometry Methods 0.000 description 1
- 235000015250 liver sausages Nutrition 0.000 description 1
- 108091070501 miRNA Proteins 0.000 description 1
- 239000002679 microRNA Substances 0.000 description 1
- 238000012015 optical character recognition Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 238000010827 pathological analysis Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229910052704 radon Inorganic materials 0.000 description 1
- SYUHGPGVQRZVTB-UHFFFAOYSA-N radon atom Chemical compound [Rn] SYUHGPGVQRZVTB-UHFFFAOYSA-N 0.000 description 1
- 238000012124 rapid diagnostic test Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 235000021067 refined food Nutrition 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000010845 search algorithm Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- JGVWCANSWKRBCS-UHFFFAOYSA-N tetramethylrhodamine thiocyanate Chemical compound [Cl-].C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=C(SC#N)C=C1C(O)=O JGVWCANSWKRBCS-UHFFFAOYSA-N 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/8483—Investigating reagent band
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/0008—Microscopes having a simple construction, e.g. portable microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/12—Condensers affording bright-field illumination
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B7/00—Mountings, adjusting means, or light-tight connections, for optical elements
- G02B7/02—Mountings, adjusting means, or light-tight connections, for optical elements for lenses
- G02B7/023—Mountings, adjusting means, or light-tight connections, for optical elements for lenses permitting adjustment
-
- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
- H04N23/50—Constructional details
- H04N23/51—Housings
-
- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
- H04N23/50—Constructional details
- H04N23/55—Optical parts specially adapted for electronic image sensors; Mounting thereof
-
- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
- H04N23/56—Cameras or camera modules comprising electronic image sensors; Control thereof provided with illuminating means
-
- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
- H04N23/57—Mechanical or electrical details of cameras or camera modules specially adapted for being embedded in other devices
-
- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
- H04N23/60—Control of cameras or camera modules
- H04N23/67—Focus control based on electronic image sensor signals
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/025—Align devices or objects to ensure defined positions relative to each other
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Optics & Photonics (AREA)
- Signal Processing (AREA)
- Multimedia (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Plasma & Fusion (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Dispersion Chemistry (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Microscoopes, Condenser (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Applications Claiming Priority (17)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762456504P | 2017-02-08 | 2017-02-08 | |
| US201762456598P | 2017-02-08 | 2017-02-08 | |
| US201762456590P | 2017-02-08 | 2017-02-08 | |
| US62/456,590 | 2017-02-08 | ||
| US62/456,504 | 2017-02-08 | ||
| US62/456,598 | 2017-02-08 | ||
| US201762456904P | 2017-02-09 | 2017-02-09 | |
| US201762457133P | 2017-02-09 | 2017-02-09 | |
| US62/456,904 | 2017-02-09 | ||
| US62/457,133 | 2017-02-09 | ||
| US201762459554P | 2017-02-15 | 2017-02-15 | |
| US62/459,554 | 2017-02-15 | ||
| US201762460075P | 2017-02-16 | 2017-02-16 | |
| US201762460062P | 2017-02-16 | 2017-02-16 | |
| US62/460,075 | 2017-02-16 | ||
| US62/460,062 | 2017-02-16 | ||
| PCT/US2018/017504 WO2018148471A2 (en) | 2017-02-08 | 2018-02-08 | Optics, device, and system for assaying |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3053009A1 true CA3053009A1 (en) | 2018-08-16 |
Family
ID=63107057
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3053009A Pending CA3053009A1 (en) | 2017-02-08 | 2018-02-08 | Optics, device, and system for assaying |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP3580597A4 (ja) |
| JP (1) | JP7177073B2 (ja) |
| CN (2) | CN116794819A (ja) |
| CA (1) | CA3053009A1 (ja) |
| WO (1) | WO2018148471A2 (ja) |
Families Citing this family (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3530752B1 (en) | 2015-04-10 | 2021-03-24 | Spatial Transcriptomics AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| USD897555S1 (en) | 2018-11-15 | 2020-09-29 | Essenlix Corporation | Assay card |
| USD898221S1 (en) | 2018-11-15 | 2020-10-06 | Essenlix Corporation | Assay plate |
| USD898224S1 (en) | 2018-11-15 | 2020-10-06 | Essenlix Corporation | Assay plate with sample landing mark |
| USD898939S1 (en) | 2018-11-20 | 2020-10-13 | Essenlix Corporation | Assay plate with sample landing mark |
| USD910202S1 (en) | 2018-11-21 | 2021-02-09 | Essenlix Corporation | Assay plate with sample landing mark |
| USD893469S1 (en) | 2018-11-21 | 2020-08-18 | Essenlix Corporation | Phone holder |
| USD910203S1 (en) | 2018-11-27 | 2021-02-09 | Essenlix Corporation | Assay plate with sample landing mark |
| USD893470S1 (en) | 2018-11-28 | 2020-08-18 | Essenlix Corporation | Phone holder |
| USD912842S1 (en) | 2018-11-29 | 2021-03-09 | Essenlix Corporation | Assay plate |
| EP3894591A2 (en) | 2018-12-10 | 2021-10-20 | 10X Genomics, Inc. | Imaging system hardware |
| USD898222S1 (en) | 2019-01-18 | 2020-10-06 | Essenlix Corporation | Assay card |
| CN110297322A (zh) * | 2019-01-21 | 2019-10-01 | 福鼎市一雄光学仪器有限公司 | 内置光纤照明的手机高清近摄放大镜头装置 |
| CN111289443A (zh) * | 2019-07-03 | 2020-06-16 | 无锡市人民医院 | 一种便于进行循环利用的引流液比色卡及其比色方法 |
| CA3143481A1 (en) * | 2019-07-11 | 2021-01-14 | Sensibility Pty Ltd | Machine learning based phone imaging system and analysis method |
| US20220276235A1 (en) * | 2019-07-18 | 2022-09-01 | Essenlix Corporation | Imaging based homogeneous assay |
| EP3798614A1 (en) * | 2019-09-30 | 2021-03-31 | IAssay, Inc. | Modular multiplex analysis devices and platforms |
| GB201918948D0 (en) | 2019-12-20 | 2020-02-05 | Oxford Immune Algorithmics Ltd | Portable device for imaging biological sample |
| CN113155555B (zh) * | 2020-01-23 | 2023-04-11 | 天津市政工程设计研究总院有限公司 | 用于模拟混凝土管廊的镁合金模型的制作方法 |
| US11768175B1 (en) | 2020-03-04 | 2023-09-26 | 10X Genomics, Inc. | Electrophoretic methods for spatial analysis |
| EP4153775A1 (en) | 2020-05-22 | 2023-03-29 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| WO2021252499A1 (en) | 2020-06-08 | 2021-12-16 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
| AU2021288090A1 (en) * | 2020-06-10 | 2023-01-19 | 10X Genomics, Inc. | Fluid delivery methods |
| US10991185B1 (en) | 2020-07-20 | 2021-04-27 | Abbott Laboratories | Digital pass verification systems and methods |
| JP2023540697A (ja) * | 2020-08-23 | 2023-09-26 | マイ－オア ダイアグノスティックス リミテッド | ヘモグロビンレベルを決定するための装置および方法 |
| AU2021343507A1 (en) * | 2020-09-18 | 2023-04-06 | 10X Genomics, Inc. | Sample handling apparatus and fluid delivery methods |
| CN112276370B (zh) * | 2020-11-27 | 2021-10-08 | 华中科技大学 | 一种基于空间光调制器的三维码激光标刻方法及系统 |
| WO2022119459A1 (en) * | 2020-12-03 | 2022-06-09 | Pictor Limited | Device and method of analyte detection |
| AU2021409136A1 (en) | 2020-12-21 | 2023-06-29 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| CN116745679A (zh) * | 2021-01-06 | 2023-09-12 | 斯科普根私人有限公司 | 紧凑型便携式多模式显微镜 |
| WO2022178054A1 (en) * | 2021-02-18 | 2022-08-25 | Peek Technologies, Inc. | Configurable diagnostic platform systems and methods for performing chemical test assays |
| CN113984759B (zh) * | 2021-09-24 | 2023-12-12 | 暨南大学 | 一种光学即时检测系统、体液玻片成晶与检测方法 |
| WO2024092682A1 (zh) * | 2022-11-04 | 2024-05-10 | 深圳华大智造科技股份有限公司 | 一种光学系统及检测方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7372985B2 (en) * | 2003-08-15 | 2008-05-13 | Massachusetts Institute Of Technology | Systems and methods for volumetric tissue scanning microscopy |
| JP2005099786A (ja) | 2003-08-29 | 2005-04-14 | Olympus Corp | 顕微鏡の光学素子切換装置 |
| DE102004034975A1 (de) * | 2004-07-16 | 2006-02-16 | Carl Zeiss Jena Gmbh | Verfahren zur Erfassung von Bildern einer Probe mit einem Mikroskop |
| JP5080186B2 (ja) * | 2007-09-26 | 2012-11-21 | 富士フイルム株式会社 | 分子分析光検出方法およびそれに用いられる分子分析光検出装置、並びにサンプルプレート |
| CN103033922A (zh) * | 2008-01-02 | 2013-04-10 | 加利福尼亚大学董事会 | 高数值孔径远程显微镜设备 |
| CN103181154A (zh) * | 2010-10-29 | 2013-06-26 | 加利福尼亚大学董事会 | 用于成像的手机显微镜装置和方法 |
| EP2656066A4 (en) | 2010-12-21 | 2017-11-08 | The Regents of The University of California | Compact wide-field fluorescent imaging on a mobile device |
| KR102230114B1 (ko) | 2013-07-12 | 2021-03-18 | 나우다이아그노스틱스, 인코포레이티드 | 트랜스-비주얼 감도를 가진 범용 신속 진단 테스트 판독기 |
| CN112033962B (zh) * | 2013-12-12 | 2024-01-12 | 梅斯医疗电子系统有限公司 | 家庭测试设备 |
| TWI551269B (zh) * | 2014-03-21 | 2016-10-01 | 財團法人工業技術研究院 | 攜帶型分析裝置以及系統 |
| TWI533025B (zh) * | 2014-07-07 | 2016-05-11 | 億觀生物科技股份有限公司 | 可攜式顯微鏡裝置 |
| CN106714670A (zh) | 2014-07-24 | 2017-05-24 | 大学健康网络 | 用于诊断目的的数据的收集和分析 |
| JP2016161550A (ja) | 2015-03-05 | 2016-09-05 | アークレイ株式会社 | 比色測定用アダプタ |
| CN204439554U (zh) * | 2015-03-27 | 2015-07-01 | 华南师范大学 | 智能手机宽场荧光成像器 |
| US10068145B2 (en) * | 2015-07-30 | 2018-09-04 | Fuji Xerox Co., Ltd. | Photographing system configured to hold a pill |
-
2018
- 2018-02-08 WO PCT/US2018/017504 patent/WO2018148471A2/en unknown
- 2018-02-08 CN CN202310507084.7A patent/CN116794819A/zh active Pending
- 2018-02-08 CN CN201880020973.8A patent/CN111465882B/zh active Active
- 2018-02-08 JP JP2019543054A patent/JP7177073B2/ja active Active
- 2018-02-08 EP EP18751754.5A patent/EP3580597A4/en active Pending
- 2018-02-08 CA CA3053009A patent/CA3053009A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2018148471A2 (en) | 2018-08-16 |
| JP2020509403A (ja) | 2020-03-26 |
| CN116794819A (zh) | 2023-09-22 |
| EP3580597A2 (en) | 2019-12-18 |
| EP3580597A4 (en) | 2021-05-05 |
| CN111465882B (zh) | 2023-05-26 |
| WO2018148471A3 (en) | 2018-10-04 |
| CN111465882A (zh) | 2020-07-28 |
| JP7177073B2 (ja) | 2022-11-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11463608B2 (en) | Image-based assay using mark-assisted machine learning | |
| CN111465882B (zh) | 用于测定的光学器件、装置和系统 | |
| JP7084959B2 (ja) | 試料、特に血液を分析するための装置及びシステム、並びにそれらの使用方法 | |
| US20200302603A1 (en) | Method of computing tumor spatial and inter-marker heterogeneity | |
| US11842483B2 (en) | Systems for cell shape estimation | |
| US10083340B2 (en) | Automated cell segmentation quality control | |
| US20240159999A1 (en) | Optics, Device, and System for Assaying and Imaging | |
| US20070135999A1 (en) | Method, apparatus and system for characterizing pathological specimen | |
| CN112689757A (zh) | 使用crof和机器学习的基于图像测定的系统和方法 | |
| JP2018502279A (ja) | 組織学画像中の核の分類 | |
| JP2021501321A (ja) | 組織および細胞染色のためのデバイスおよび方法 | |
| US11906432B2 (en) | Enhanced, rapid, homogeneous, cell staining and assay | |
| US20220407988A1 (en) | Image-Based Assay Using Mark-Assisted Machine Learning | |
| WO2009063185A1 (en) | Test results reading method and apparatus | |
| Dolled-Filhart et al. | Automated analysis of tissue microarrays | |
| US20220065795A1 (en) | System and methods for remote assessment of a sample assay for disease diagnostics | |
| US20090041633A1 (en) | Apparatus and method for performing ligand binding assays on microarrays in multiwell plates | |
| US7867783B2 (en) | Apparatus and method for performing ligand binding assays on microarrays in multiwell plates |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20220920 |
|
| EEER | Examination request |
Effective date: 20220920 |
|
| EEER | Examination request |
Effective date: 20220920 |
|
| EEER | Examination request |
Effective date: 20220920 |
|
| EEER | Examination request |
Effective date: 20220920 |
|
| EEER | Examination request |
Effective date: 20220920 |