CA3051141C - Media and methods for enhancing the survival and proliferation of stem cells - Google Patents

Media and methods for enhancing the survival and proliferation of stem cells Download PDF

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Publication number
CA3051141C
CA3051141C CA3051141A CA3051141A CA3051141C CA 3051141 C CA3051141 C CA 3051141C CA 3051141 A CA3051141 A CA 3051141A CA 3051141 A CA3051141 A CA 3051141A CA 3051141 C CA3051141 C CA 3051141C
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Canada
Prior art keywords
acid
stem cells
lipid
culture medium
cells
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CA3051141A
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English (en)
French (fr)
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CA3051141A1 (en
Inventor
Adam HIRST
Melanie KARDEL
Wing CHANG
Michael Hiatt
Eric Jervis
Arwen HUNTER
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Stemcell Technologies Canada Inc
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Stemcell Technologies Canada Inc
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Application filed by Stemcell Technologies Canada Inc filed Critical Stemcell Technologies Canada Inc
Publication of CA3051141A1 publication Critical patent/CA3051141A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Reproductive Health (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA3051141A 2017-01-23 2018-01-23 Media and methods for enhancing the survival and proliferation of stem cells Active CA3051141C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201762449413P 2017-01-23 2017-01-23
US62/449,413 2017-01-23
US201762518776P 2017-06-13 2017-06-13
US62/518,776 2017-06-13
US201762608875P 2017-12-21 2017-12-21
US62/608,875 2017-12-21
PCT/CA2018/050076 WO2018132926A1 (en) 2017-01-23 2018-01-23 Media and methods for enhancing the survival and proliferation of stem cells

Publications (2)

Publication Number Publication Date
CA3051141A1 CA3051141A1 (en) 2018-07-26
CA3051141C true CA3051141C (en) 2021-06-22

Family

ID=62907538

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3051141A Active CA3051141C (en) 2017-01-23 2018-01-23 Media and methods for enhancing the survival and proliferation of stem cells

Country Status (9)

Country Link
US (2) US11697796B2 (https=)
EP (2) EP4361256A3 (https=)
JP (1) JP7049347B2 (https=)
CN (1) CN110462025B (https=)
AU (1) AU2018209127B2 (https=)
CA (1) CA3051141C (https=)
IL (1) IL268194B (https=)
SG (1) SG11201906731XA (https=)
WO (1) WO2018132926A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11697796B2 (en) 2017-01-23 2023-07-11 Stemcell Technologies Canada Inc. Media and methods for enhancing the survival and proliferation of stem cells
CN111808797A (zh) * 2020-07-28 2020-10-23 湖南赛诺生物科技股份有限公司 Necrostatin-1的应用及促进新生猪胰岛细胞成熟的制剂
JP2022032793A (ja) * 2020-08-14 2022-02-25 アルブキュラ コーポレーション アルブミン組成物並びにそれを製造及び使用する方法
AU2021337864A1 (en) 2020-09-02 2023-05-04 Yztherapeutic Performance Ltd. Cellular composition for treatment of diseases, disorders or conditions and method of use
WO2022114724A1 (ko) * 2020-11-27 2022-06-02 (주)엑셀세라퓨틱스 고효율 및 고순도 엑소좀 생산용 배지 조성물
CN116867892A (zh) * 2021-02-15 2023-10-10 凸版印刷株式会社 三维组织体的制造方法和脂肪来源干细胞的分化促进方法
CN116121186A (zh) * 2023-01-03 2023-05-16 内蒙古奥普赛生物科技有限公司 一种利用棕榈油酸培养脂肪间充质干细胞的方法
EP4698630A1 (en) * 2023-04-20 2026-02-25 Stemcell Technologies Canada Inc. Methods for large scale expansion of pluripotent stem cells (pscs)
CN116814532B (zh) * 2023-07-10 2025-02-11 上海迈邦生物科技有限公司 一种用于哺乳动物细胞培养基的脂类复合物及其制备方法与应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2277278A1 (en) 1997-01-10 1998-07-16 Life Technologies, Inc. Embryonic stem cell serum replacement
ATE549395T1 (de) 2004-09-08 2012-03-15 Wisconsin Alumni Res Found Medium und kultur von embryonalen stammzellen
US9074176B2 (en) * 2006-01-13 2015-07-07 Two Cells Co., Ltd. Culture medium additive for use in serum-free culturing of animal cell, kit and use thereof
US8241908B2 (en) 2006-05-16 2012-08-14 Becton, Dickinson And Company Extracellular matrix coated surface for culturing cells
GB0613756D0 (en) 2006-07-12 2006-08-23 Univ Sheffield Cell culture medium
ES2360782B1 (es) 2009-07-28 2012-03-12 Grifols, S.A. Medios para cultivo de células de mamíferos que comprenden sobrenadante de etapas del fraccionamiento de Cohn y uso de los mismos.
FI20096288A0 (fi) 2009-12-04 2009-12-04 Kristiina Rajala Formulations and methods for culturing stem cells
CA2786331C (en) 2010-01-06 2018-05-01 Ervin Industries, Inc. Frangible, ceramic-metal composite objects and methods of making the same
JP6148429B2 (ja) 2011-01-31 2017-06-14 協和発酵バイオ株式会社 ヒト多能性幹細胞の培養方法
US8497124B2 (en) * 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
CA2866590A1 (en) 2012-03-07 2013-09-12 Janssen Biotech, Inc. Defined media for expansion and maintenance of pluripotent stem cells
CN105531365A (zh) * 2013-04-23 2016-04-27 耶达研究及发展有限公司 分离的幼稚多能干细胞和产生所述细胞的方法
SG11201509767UA (en) 2013-05-30 2015-12-30 Ajinomoto Kk Medium for culturing stem cells
WO2015042356A1 (en) 2013-09-19 2015-03-26 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Chemically defined culture medium for stem cell maintenance and differentiation
JP6780638B2 (ja) * 2015-03-30 2020-11-04 味の素株式会社 ヒト血清アルブミンを含む神経幹細胞増殖培地
US11697796B2 (en) 2017-01-23 2023-07-11 Stemcell Technologies Canada Inc. Media and methods for enhancing the survival and proliferation of stem cells

Also Published As

Publication number Publication date
AU2018209127A1 (en) 2019-08-08
IL268194B (en) 2022-05-01
CN110462025A (zh) 2019-11-15
US20200017825A1 (en) 2020-01-16
SG11201906731XA (en) 2019-08-27
US20230250392A1 (en) 2023-08-10
EP3571290A4 (en) 2020-10-28
CA3051141A1 (en) 2018-07-26
EP3571290B1 (en) 2024-04-10
JP7049347B2 (ja) 2022-04-06
IL268194A (en) 2019-09-26
EP3571290A1 (en) 2019-11-27
US11697796B2 (en) 2023-07-11
CN110462025B (zh) 2023-11-21
JP2020505038A (ja) 2020-02-20
EP4361256A3 (en) 2024-07-31
WO2018132926A1 (en) 2018-07-26
AU2018209127B2 (en) 2021-10-21
EP4361256A2 (en) 2024-05-01

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