CA3027616A1 - Cell culture device and methods - Google Patents

Cell culture device and methods Download PDF

Info

Publication number
CA3027616A1
CA3027616A1 CA3027616A CA3027616A CA3027616A1 CA 3027616 A1 CA3027616 A1 CA 3027616A1 CA 3027616 A CA3027616 A CA 3027616A CA 3027616 A CA3027616 A CA 3027616A CA 3027616 A1 CA3027616 A1 CA 3027616A1
Authority
CA
Canada
Prior art keywords
microfluidic
cells
gel
cell culture
organoid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3027616A
Other languages
English (en)
French (fr)
Inventor
Paul Vulto
Sebastiaan Johannes TRIETSCH
Arnaud Nicolas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mimetas BV
Original Assignee
Mimetas BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from NL2016965A external-priority patent/NL2016965B1/en
Application filed by Mimetas BV filed Critical Mimetas BV
Publication of CA3027616A1 publication Critical patent/CA3027616A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C12N5/0691—Vascular smooth muscle cells; 3D culture thereof, e.g. models of blood vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0858—Side walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/28—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from vascular endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Vascular Medicine (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3027616A 2016-06-15 2017-06-12 Cell culture device and methods Pending CA3027616A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
NL2016965 2016-06-15
NL2016965A NL2016965B1 (en) 2016-06-15 2016-06-15 Cell culture device.
NL2018033 2016-12-21
NL2018033 2016-12-21
PCT/EP2017/064302 WO2017216113A2 (en) 2016-06-15 2017-06-12 Cell culture device and methods

Publications (1)

Publication Number Publication Date
CA3027616A1 true CA3027616A1 (en) 2017-12-21

Family

ID=59215734

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3027616A Pending CA3027616A1 (en) 2016-06-15 2017-06-12 Cell culture device and methods

Country Status (9)

Country Link
US (1) US11629319B2 (cg-RX-API-DMAC10.html)
EP (1) EP3472301B1 (cg-RX-API-DMAC10.html)
JP (1) JP6967535B2 (cg-RX-API-DMAC10.html)
CN (1) CN109804057B (cg-RX-API-DMAC10.html)
AU (1) AU2017286096B2 (cg-RX-API-DMAC10.html)
BR (1) BR112018075920A2 (cg-RX-API-DMAC10.html)
CA (1) CA3027616A1 (cg-RX-API-DMAC10.html)
RU (1) RU2741806C2 (cg-RX-API-DMAC10.html)
WO (1) WO2017216113A2 (cg-RX-API-DMAC10.html)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3966312A4 (en) * 2019-05-10 2023-06-21 Oregon Health & Science University MANIPULATED BONE MARROW MODEL

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11760965B2 (en) 2017-10-19 2023-09-19 Georgia Tech Research Corporation Mesofluidic device for culture of cell aggregates
NL2020518B1 (en) 2018-03-02 2019-09-12 Mimetas B V Device and method for performing electrical measurements
CN108486035B (zh) * 2018-03-26 2021-11-26 西北大学 一种三维类器官的液滴培养方法
NL2022085B1 (en) * 2018-11-28 2020-06-25 Mimetas B V Device for assessing mechanical strain induced in or by cells
KR102209346B1 (ko) * 2019-03-13 2021-01-29 재단법인대구경북과학기술원 미세관통막 기반의 수화젤 마이크로웰 어레이 및 이를 이용한 3차원 단일세포 분리 및 배양방법
US12024696B2 (en) * 2019-05-08 2024-07-02 Molecular Devices (Austria) GmbH System and method for organoid culture
CA3142113A1 (en) * 2019-05-28 2020-12-03 Xiling Shen Methods and apparatuses for patient-derived micro-organospheres
KR102233992B1 (ko) * 2019-06-28 2021-03-29 건양대학교 산학협력단 면역화학진단 세포 칩 구조체
US11767498B2 (en) * 2019-08-28 2023-09-26 Massachusetts Institute Of Technology In vitro tissue plate
CN110564674B (zh) * 2019-09-29 2021-05-11 广东工业大学 一种干细胞铺底工作液及其应用
JP7738296B2 (ja) * 2019-10-17 2025-09-12 公立大学法人横浜市立大学 薬物毒性評価方法
NL2024202B1 (en) * 2019-11-08 2021-07-20 Mimetas B V Microfluidic cell culture system
AU2020399785A1 (en) * 2019-12-12 2022-06-30 The Walter And Eliza Hall Institute Of Medical Research Organoid cultures
EP3907007A1 (de) 2020-05-08 2021-11-10 Technische Universität Wien Mikrofluidische vorrichtung
US20210394177A1 (en) * 2020-06-19 2021-12-23 The Texas A&M University System Systems and methods for mimicking a blood vessel of a patient
NL2026038B1 (en) * 2020-07-09 2022-03-15 Mimetas B V Microfluidic cell culture device
CN112143642B (zh) * 2020-08-28 2022-06-14 上海交通大学 用于体外培养的血管化肿瘤微流控器官芯片及其制备方法
CA3246410A1 (en) 2020-09-04 2025-10-31 9493662 Canada Inc. Multi-well device, kits and methods for analysis of cells
CN111996121B (zh) * 2020-09-30 2025-05-30 北京大橡科技有限公司 3d多器官共培养芯片
JP7565008B2 (ja) * 2020-10-07 2024-10-10 ウシオ電機株式会社 マイクロ流体デバイス及びマイクロ流体デバイスの使用方法
EP4232553A1 (en) * 2020-10-22 2023-08-30 Molecular Devices (Austria) GmbH Microplates for automating organoid cultivation
EP4232543A4 (en) * 2020-10-22 2024-10-09 The Board of Regents of the University of Texas System HIGH-THROUGHPUT CELL MIGRATION ASSAY PLATES AND MANUFACTURING METHODS
CN112358966B (zh) * 2020-10-23 2022-03-22 上海交通大学 一种滑动式微流控器官芯片及其制备方法
US20230407220A1 (en) * 2020-11-20 2023-12-21 The Regents Of The University Of California Novel tissue culture systems and reduced gravity culture method for the production of vascularized tissue
CN117157147A (zh) * 2020-12-01 2023-12-01 新加坡科技研究局 用于血管化细胞聚集体的装置和方法
WO2022175898A1 (en) * 2021-02-19 2022-08-25 Molecular Devices (Austria) GmbH Methods for organoid passaging using microplate well units
EP4308684A4 (en) * 2021-03-16 2025-11-26 Agency Science Tech & Res WELL INSERT AND DEVICE FOR 3D CELL CULTURE AND IN VITRO TISSUE MODELING
WO2022216132A1 (ko) * 2021-04-09 2022-10-13 연세대학교 산학협력단 세포 집합체 배양을 위한 미세유체 현적배양 디바이스
US12480079B2 (en) 2021-04-23 2025-11-25 Massachusetts Institute Of Technology Fluidic platforms for perfusable vascularized tissues with infiltrates
NL2028424B1 (en) * 2021-06-10 2022-12-20 Mimetas B V Method and apparatus for forming a microfluidic gel structure
CN113755328A (zh) * 2021-09-08 2021-12-07 中山大学 一种类器官培养装置及类器官培养方法
WO2023073178A1 (en) * 2021-10-29 2023-05-04 Universität Bern Microfluidic cell culturing device
USD1028280S1 (en) 2021-12-27 2024-05-21 Molecular Devices (Austria) GmbH Organoid microplate
US12544754B2 (en) 2022-01-18 2026-02-10 Molecular Devices (Austria) GmbH 3D printing of organoid passaging plate
US12360102B2 (en) 2022-01-28 2025-07-15 Glaxosmithkline Intellectual Property Development Limited High-throughput assay for cell migration, chemotaxis, and function
CN114591836A (zh) * 2022-02-18 2022-06-07 清华大学深圳国际研究生院 体外血管床微流控芯片及其应用
CN119301240A (zh) * 2022-03-18 2025-01-10 分子装置(奥地利)有限公司 使用分离孔微孔板的细胞培养应用方法
US20250236825A1 (en) * 2022-03-21 2025-07-24 Molecular Devices (Austria) GmbH Systems and methods for co-culture in microplates
WO2023218024A1 (en) * 2022-05-13 2023-11-16 Ecole Polytechnique Federale De Lausanne (Epfl) System for automated and dynamic cell culture
US20230383225A1 (en) * 2022-05-27 2023-11-30 University Of Utah Research Foundation Lung-on-chip device with integrated extracellular matrix membrane
DE102022209416B3 (de) * 2022-09-09 2023-12-21 Robert Bosch Gesellschaft mit beschränkter Haftung Mikrofluidische Vorrichtung
US20260201341A1 (en) * 2022-12-14 2026-07-16 President And Fellows Of Harvard College Organoids in extracellular matrix droplets
DE102023200837A1 (de) * 2023-02-02 2024-08-08 Carl Zeiss Ag Probengefäß zur Kultivierung biologischer Proben, Vorrichtung zu dessen Betrieb und Mikroskop
WO2024182622A1 (en) * 2023-03-01 2024-09-06 Research Institute At Nationwide Children's Hospital Systems, devices, and processes for miniaturized physiological mimicry
NL2034328B1 (en) 2023-03-13 2024-09-24 Mimetas B V Liver vasculature
CN120883055A (zh) 2023-03-13 2025-10-31 米梅塔斯公司 肝脉管系统
CN117586876B (zh) * 2023-10-07 2024-10-25 丹望医疗科技(上海)有限公司 一种血管化类器官芯片及血管化类器官
WO2025080829A1 (en) * 2023-10-10 2025-04-17 The Trustees Of The University Of Pennsylvania A model of the blood-brain barrier on a chip
WO2025132381A1 (en) * 2023-12-21 2025-06-26 F. Hoffmann-La Roche Ag Cell culturing device
WO2025186340A1 (de) * 2024-03-05 2025-09-12 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Vorrichtung zum erzeugen von drei-dimensionalen zellstrukturen in einem hydrogel, mikrosystem und/oder mikrofluidische zellkulturplattform und verfahren zum herstellen der vorrichtung
CN118374350A (zh) * 2024-04-15 2024-07-23 丹望医疗科技(上海)有限公司 血管化类器官芯片及其应用
CN118086053B (zh) * 2024-04-29 2024-07-09 四川迪亚生物科技集团有限公司 一种自灌流培养板及使用方法
TR2024007044A1 (tr) * 2024-06-04 2025-12-22 Izmir Yueksek Teknoloji Enstituesue Rektoerluegue 2 ve 3 boyutlu hücre ve doku kültürünleri̇nde kullanilmak üzere üstü açik bi̇r mi̇krofi̇zyoloji̇k si̇stem ve bu si̇stemi̇n çalişma yöntemi̇
GB202409293D0 (en) 2024-06-27 2024-08-14 Momo Biotech Ltd Device
CN120591192A (zh) * 2024-07-15 2025-09-05 苏州先觉生物科技有限公司 一种基于牺牲材料的3d细胞培养体的制备方法、3d细胞培养体及应用
CN119060843A (zh) * 2024-09-04 2024-12-03 上海交通大学 用于血管化肿瘤类器官培养的高通量器官芯片及制备方法
CN122141014B (zh) * 2026-05-11 2026-07-17 山东第一医科大学附属省立医院(山东省立医院) 一种负载血管化鼻黏膜类器官微胶囊的制备方法及其应用

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4829000A (en) 1985-08-30 1989-05-09 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Reconstituted basement membrane complex with biological activity
US7534601B2 (en) * 2002-08-27 2009-05-19 Vanderbilt University Capillary perfused bioreactors with multiple chambers
GB0321158D0 (en) * 2003-09-10 2003-10-08 Central Research Lab Ltd Apparatus and method for handling cells,embryos or oocytes
EP3029135B1 (en) 2005-07-07 2021-03-17 The Regents of the University of California Apparatus for cell culture array
US20110129850A1 (en) 2006-12-22 2011-06-02 UCLA Office of Intellectual Property Microfluidic platform for cell culture and assay
US8114289B2 (en) 2008-01-29 2012-02-14 California Institute Of Technology Method and apparatus for microfiltration to perform cell separation
EP2279238A2 (en) * 2008-04-21 2011-02-02 Cell Kinetics, Ltd. Flat cell carriers with cell traps
CA2727003C (en) 2008-06-04 2018-09-18 Uwe Marx Organ-on-a-chip-device
KR102667365B1 (ko) * 2008-07-16 2024-05-21 칠드런'즈 메디컬 센터 코포레이션 마이크로채널을 갖는 기관 모방 장치 및 그 사용 및 제조 방법
CN102112594A (zh) * 2008-08-01 2011-06-29 智能生物系统公司 细胞培养系统的样品端口
GB0815427D0 (en) * 2008-08-22 2008-10-01 Capsant Neurotechnologies S A Device
ES2401640T3 (es) 2008-09-22 2013-04-23 University Of Zurich Prorektorat Forschung Placa para gotas en suspensión
WO2010062911A2 (en) 2008-11-26 2010-06-03 Hurel Corporation Compositions and methods of functionally enhanced in vitro cell culture system
EP2213364A1 (en) 2009-01-30 2010-08-04 Albert-Ludwigs-Universität Freiburg Phase guide patterns for liquid manipulation
JP2010253572A (ja) 2009-04-21 2010-11-11 Makita Corp 切断機における割り刃の固定具脱落防止構造
GB0910330D0 (en) * 2009-06-16 2009-07-29 Univ Leiden A biological microfluidics chip and related methods
EP2622342A4 (en) 2010-09-29 2016-12-14 Massachusetts Inst Technology DEVICE FOR THE INVESTIGATION OF CELLULAR INTERACTIONS WITH HIGH THROUGHPUT
EP2681306B1 (en) 2011-02-28 2019-01-09 President and Fellows of Harvard College Cell culture system
GB201103917D0 (en) 2011-03-08 2011-04-20 Univ Leiden Apparatus for and methods of processing liquids or liquid based substances
WO2013062485A1 (en) * 2011-10-24 2013-05-02 Agency For Science, Technology And Research Microfluidic device and microfluidic system
US20140335496A1 (en) 2011-12-05 2014-11-13 Research Triangle Institute Human conducting airway model comprising multiple fluidic pathways
WO2013134778A1 (en) * 2012-03-09 2013-09-12 Si-Bone Inc. Impactor
SG10201803961VA (en) 2012-04-01 2018-07-30 Emd Millipore Corp Cell culture and gradient migration assay methods and devices
GB2505706A (en) * 2012-09-10 2014-03-12 Univ Leiden Apparatus comprising meniscus alignment barriers
US10012640B2 (en) * 2013-02-14 2018-07-03 Cfd Research Corporation Cell culture device with an array of microfluidic networks
EP2811012A1 (en) 2013-06-07 2014-12-10 Eidgenossisch Technische Hochschule Zürich Hanging network plate
PL3041926T3 (pl) * 2013-09-05 2020-11-02 Universität Bern Urządzenie do modelowania in-vitro tkanek organów in-vivo
GB2584034B (en) * 2013-12-20 2021-03-24 Harvard College Organomimetic devices and methods of use and manufacturing thereof
US20160130543A1 (en) 2014-11-10 2016-05-12 The Government Of The United States Of America, As Represented By The Secretary Of The Navy Modular Microtube Network for Vascularized Organ-On-A-Chip Models
WO2016081751A1 (en) 2014-11-19 2016-05-26 Nortis, Inc. Method for vascularizing in-vitro generated or ex-vivo tissue fragments in a microfluidic device
RU2584598C1 (ru) * 2015-03-27 2016-05-20 Общество с ограниченной ответственностью Научно-технический центр "БиоКлиникум" Микрофлюидный чип для создания клеточных моделей органов млекопитающих
CN104877905A (zh) 2015-05-04 2015-09-02 清华大学深圳研究生院 细胞体外共培养微流控芯片、系统及方法
NL2014840B1 (en) * 2015-05-21 2017-01-31 Intecrypt B V Microfluidic device for in vitro 3D cell culture experimentation.
CN107847929B (zh) 2015-06-05 2020-08-11 米梅塔斯私人有限公司 微流体板

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3966312A4 (en) * 2019-05-10 2023-06-21 Oregon Health & Science University MANIPULATED BONE MARROW MODEL

Also Published As

Publication number Publication date
CN109804057A (zh) 2019-05-24
RU2741806C2 (ru) 2021-01-28
AU2017286096A1 (en) 2019-01-17
WO2017216113A2 (en) 2017-12-21
JP6967535B2 (ja) 2021-11-17
AU2017286096B2 (en) 2022-01-20
CN109804057B (zh) 2022-09-09
RU2019100603A (ru) 2020-07-15
US20200063081A1 (en) 2020-02-27
WO2017216113A3 (en) 2018-02-01
US11629319B2 (en) 2023-04-18
EP3472301C0 (en) 2023-08-02
EP3472301B1 (en) 2023-08-02
BR112018075920A2 (pt) 2019-03-26
EP3472301A2 (en) 2019-04-24
RU2019100603A3 (cg-RX-API-DMAC10.html) 2020-08-12
JP2019517808A (ja) 2019-06-27

Similar Documents

Publication Publication Date Title
US11629319B2 (en) Cell culture device and methods
NL2022085B1 (en) Device for assessing mechanical strain induced in or by cells
US9121847B2 (en) Three-dimensional microfluidic platforms and methods of use thereof
Wang et al. In vitro generation of colonic epithelium from primary cells guided by microstructures
Wang et al. Capture and 3D culture of colonic crypts and colonoids in a microarray platform
AU2022289996B2 (en) Method and apparatus for forming a microfluidic gel structure
EP4148117A1 (en) Cell culture apparatus, methods for cell cultivation by using the same, cell culture incubator comprising the same, and uses of the cell culture apparatus
CA2727003A1 (en) Organ-on-a-chip-device
Wang et al. 3D anastomosed microvascular network model with living capillary networks and endothelial cell-lined microfluidic channels
US20260117161A1 (en) Cell culture apparatus, methods for cell cultivation by using the same, cell culture incubator comprising the same, and uses of the cell culture apparatus
NL2016965B1 (en) Cell culture device.
NL2026038B1 (en) Microfluidic cell culture device
NL2035770B1 (en) Titerplate
US20260109925A1 (en) Microfluidic device for forming three-dimensional tissue barrier
CN117957303A (zh) 细胞培养装置、使用该细胞培养装置的细胞培养方法、包括该细胞培养装置的细胞培养孵箱以及该细胞培养装置的用途
IT202100025460A1 (it) Dispositivo lab-on-chip per studiare la migrazione cellulare in sistemi tridimensionali e relativo metodo di utilizzo
Wang et al. 3D anastomosed microvascular network model with living capillary

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20220509

D15 Examination report completed

Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D15-D126 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: EXAMINER'S REPORT

Effective date: 20241204

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT RECEIVED - RESPONSE TO EXAMINER'S REQUISITION

Effective date: 20250402

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT

Effective date: 20250407

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 8TH ANNIV.) - STANDARD

Year of fee payment: 8

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20250428

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20250428

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT DETERMINED COMPLIANT

Effective date: 20250717

P13 Application amended

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P13-X000 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: APPLICATION AMENDED

Effective date: 20250717

D15 Examination report completed

Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D15-D126 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: EXAMINER'S REPORT

Effective date: 20260218

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 9TH ANNIV.) - STANDARD

Year of fee payment: 9

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20260604

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20260604

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT RECEIVED - RESPONSE TO EXAMINER'S REQUISITION

Effective date: 20260609

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT

Effective date: 20260610