CA3022431C - Method of nucleic acid sequence determination - Google Patents
Method of nucleic acid sequence determination Download PDFInfo
- Publication number
- CA3022431C CA3022431C CA3022431A CA3022431A CA3022431C CA 3022431 C CA3022431 C CA 3022431C CA 3022431 A CA3022431 A CA 3022431A CA 3022431 A CA3022431 A CA 3022431A CA 3022431 C CA3022431 C CA 3022431C
- Authority
- CA
- Canada
- Prior art keywords
- nucleotide
- nucleic acid
- polymerase
- dna polymerase
- template nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/101—DNA polymerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/518—Detection characterised by immobilisation to a surface characterised by the immobilisation of the nucleic acid sample or target
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662329489P | 2016-04-29 | 2016-04-29 | |
| US62/329,489 | 2016-04-29 | ||
| US201762444733P | 2017-01-10 | 2017-01-10 | |
| US62/444,733 | 2017-01-10 | ||
| PCT/US2017/030135 WO2017190012A1 (en) | 2016-04-29 | 2017-04-28 | Method of nucleic acid sequence determination |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA3022431A1 CA3022431A1 (en) | 2017-11-02 |
| CA3022431C true CA3022431C (en) | 2023-03-21 |
Family
ID=58672825
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3022431A Active CA3022431C (en) | 2016-04-29 | 2017-04-28 | Method of nucleic acid sequence determination |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US10584379B2 (cg-RX-API-DMAC10.html) |
| EP (1) | EP3449011B1 (cg-RX-API-DMAC10.html) |
| JP (1) | JP6915939B2 (cg-RX-API-DMAC10.html) |
| KR (1) | KR102231977B1 (cg-RX-API-DMAC10.html) |
| CN (1) | CN109154024B (cg-RX-API-DMAC10.html) |
| AU (1) | AU2017258523B2 (cg-RX-API-DMAC10.html) |
| CA (1) | CA3022431C (cg-RX-API-DMAC10.html) |
| SG (1) | SG11201809522WA (cg-RX-API-DMAC10.html) |
| WO (1) | WO2017190012A1 (cg-RX-API-DMAC10.html) |
Families Citing this family (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10597643B2 (en) | 2016-04-29 | 2020-03-24 | Omniome, Inc. | Polymerases engineered to reduce nucleotide-independent DNA binding |
| CN109154024B (zh) | 2016-04-29 | 2022-03-04 | 欧姆尼欧美公司 | 核酸序列测定方法 |
| US10443098B2 (en) * | 2016-08-15 | 2019-10-15 | Omniome, Inc. | Method and system for sequencing nucleic acids |
| AU2018210944B2 (en) * | 2017-01-20 | 2021-07-22 | Pacific Biosciences Of California, Inc. | Process for cognate nucleotide detection in a nucleic acid sequencing workflow |
| US10161003B2 (en) | 2017-04-25 | 2018-12-25 | Omniome, Inc. | Methods and apparatus that increase sequencing-by-binding efficiency |
| WO2019079593A1 (en) | 2017-10-19 | 2019-04-25 | Omniome, Inc. | BACKGROUND NOISE REDUCTION AND STABILIZATION OF SIMULTANEOUS COMPLEXES IN LINKED ASSAY WORKFLOWS |
| RU2751359C2 (ru) | 2018-02-16 | 2021-07-13 | Иллюмина, Инк. | Нуклеотиды, меченные зарядом, и способы их применения |
| JP7554117B2 (ja) | 2018-04-26 | 2024-09-19 | パシフィック・バイオサイエンシズ・オブ・カリフォルニア・インク． | 核酸－ヌクレオチド－ポリメラーゼ複合体を安定化するための方法及び組成物 |
| KR20210045361A (ko) | 2018-05-17 | 2021-04-26 | 레커그니션 어낼러틱스 엘엘씨 | 효소 활성의 직접 전기적 측정을 위한 장치, 시스템 및 방법 |
| JP2021525078A (ja) | 2018-05-31 | 2021-09-24 | オムニオム インコーポレイテッドＯｍｎｉｏｍｅ， Ｉｎｃ． | 核酸配列決定におけるシグナル対ノイズの増加 |
| AU2019312152A1 (en) | 2018-07-24 | 2021-02-18 | Pacific Biosciences Of California, Inc. | Serial formation of ternary complex species |
| US11242512B2 (en) | 2018-09-17 | 2022-02-08 | Omniome, Inc. | Engineered polymerases for improved sequencing |
| US10768173B1 (en) | 2019-09-06 | 2020-09-08 | Element Biosciences, Inc. | Multivalent binding composition for nucleic acid analysis |
| US10704094B1 (en) | 2018-11-14 | 2020-07-07 | Element Biosciences, Inc. | Multipart reagents having increased avidity for polymerase binding |
| US10876148B2 (en) | 2018-11-14 | 2020-12-29 | Element Biosciences, Inc. | De novo surface preparation and uses thereof |
| US20240011086A1 (en) | 2018-11-15 | 2024-01-11 | Omniome, Inc. | Electronic detection of nucleic acid structure |
| CN211311438U (zh) | 2018-12-04 | 2020-08-21 | 欧姆尼欧美公司 | 流动池 |
| EP3899032A2 (en) | 2018-12-20 | 2021-10-27 | Omniome, Inc. | Temperature control for analysis of nucleic acids and other analytes |
| US12351855B2 (en) | 2019-01-30 | 2025-07-08 | Arizona Board Of Regents On Behalf Of Arizona State University | Bioelectronic circuits, systems and methods for preparing and using them |
| US11499189B2 (en) | 2019-02-14 | 2022-11-15 | Pacific Biosciences Of California, Inc. | Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes |
| US12313627B2 (en) | 2019-05-01 | 2025-05-27 | Element Biosciences, Inc. | Multivalent binding composition for nucleic acid analysis |
| WO2020242901A1 (en) * | 2019-05-24 | 2020-12-03 | Element Biosciences, Inc. | Polymerase-nucleotide conjugates for sequencing by trapping |
| CA3140279A1 (en) * | 2019-06-20 | 2020-12-24 | Arizona Board Of Regents On Behalf Of Arizona State University | Direct electrical readout of nucleic acid sequences |
| JP2022537069A (ja) * | 2019-06-21 | 2022-08-23 | サーモ フィッシャー サイエンティフィック バルティックス ユーエービー | 次世代配列決定ライブラリを調製するための核酸の標識化に有用なオリゴヌクレオチドが連結された三リン酸ヌクレオチド |
| EP4028407B1 (en) | 2019-09-10 | 2023-08-16 | Pacific Biosciences of California, Inc. | Reversible modification of nucleotides |
| US11287422B2 (en) | 2019-09-23 | 2022-03-29 | Element Biosciences, Inc. | Multivalent binding composition for nucleic acid analysis |
| WO2021076152A1 (en) | 2019-10-18 | 2021-04-22 | Omniome, Inc. | Methods and compositions for capping nucleic acids |
| US20210139867A1 (en) | 2019-11-08 | 2021-05-13 | Omniome, Inc. | Engineered polymerases for improved sequencing by binding |
| DE202019106695U1 (de) | 2019-12-02 | 2020-03-19 | Omniome, Inc. | System zur Sequenzierung von Nukleinsäuren in Fluidschaum |
| DE202019106694U1 (de) | 2019-12-02 | 2020-03-19 | Omniome, Inc. | System zur Sequenzierung von Nukleinsäuren in Fluidschaum |
| EP4114966B1 (en) | 2020-03-03 | 2025-02-26 | Pacific Biosciences Of California, Inc. | Methods and compositions for sequencing double stranded nucleic acids |
| JP2023524079A (ja) | 2020-04-30 | 2023-06-08 | アリゾナ・ボード・オブ・リージェンツ・オン・ビハーフ・オブ・アリゾナ・ステイト・ユニバーシティー | 生体高分子の配列決定のための方法 |
| CN115836135A (zh) | 2020-05-05 | 2023-03-21 | 加利福尼亚太平洋生物科学股份有限公司 | 用于修饰聚合酶-核酸复合物的组合物和方法 |
| JP2023532231A (ja) * | 2020-06-30 | 2023-07-27 | イルミナ インコーポレイテッド | 傷なしｄｎａを生成するための触媒的に制御された合成による配列決定 |
| WO2022240764A1 (en) | 2021-05-10 | 2022-11-17 | Pacific Biosciences Of California, Inc. | Single-molecule seeding and amplification on a surface |
| WO2022240766A1 (en) | 2021-05-10 | 2022-11-17 | Pacific Biosciences Of California, Inc. | Dna amplification buffer replenishment during rolling circle amplification |
| EP4352244A4 (en) | 2021-06-07 | 2025-04-16 | Arizona Board of Regents on behalf of Arizona State University | Methods for sequencing biopolymers |
| CN119061125A (zh) | 2021-07-21 | 2024-12-03 | 元素生物科学公司 | 用于核酸测序的方法 |
| WO2023035108A1 (zh) * | 2021-09-07 | 2023-03-16 | 深圳华大智造科技股份有限公司 | 一种用于分析靶多核苷酸的序列的方法 |
| WO2023081485A1 (en) * | 2021-11-08 | 2023-05-11 | Pacific Biosciences Of California, Inc. | Stepwise sequencing of a polynucleotide with a homogenous reaction mixture |
| CA3264896A1 (en) | 2022-08-15 | 2024-02-22 | Element Biosciences, Inc. | SPATIAL RESOLUTION SURFACE CAPTURE OF NUCLEIC ACIDS |
| EP4627115A1 (en) | 2022-11-29 | 2025-10-08 | Element Biosciences, Inc. | Flow cell devices and optical systems for nucleic acid sequencing |
| EP4739790A1 (en) | 2023-07-06 | 2026-05-13 | Pacific Biosciences of California, Inc. | Methods and compositions for stabilizing concatemers |
| WO2025244989A1 (en) | 2024-05-21 | 2025-11-27 | Pacific Biosciences Of California, Inc. | Selectively functionalized nanoscale wells |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991006678A1 (en) | 1989-10-26 | 1991-05-16 | Sri International | Dna sequencing |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| US7414116B2 (en) | 2002-08-23 | 2008-08-19 | Illumina Cambridge Limited | Labelled nucleotides |
| AU2003269991A1 (en) * | 2003-08-20 | 2005-03-10 | Applera Corporation | Polymerase compositions |
| US8071755B2 (en) | 2004-05-25 | 2011-12-06 | Helicos Biosciences Corporation | Nucleotide analogs |
| AU2005254984B2 (en) * | 2004-06-10 | 2008-10-16 | Ge Healthcare Bio-Sciences Corp. | Method for nucleic acid analysis |
| US7264934B2 (en) | 2004-06-10 | 2007-09-04 | Ge Healthcare Bio-Sciences Corp. | Rapid parallel nucleic acid analysis |
| US7315019B2 (en) | 2004-09-17 | 2008-01-01 | Pacific Biosciences Of California, Inc. | Arrays of optical confinements and uses thereof |
| US7482120B2 (en) | 2005-01-28 | 2009-01-27 | Helicos Biosciences Corporation | Methods and compositions for improving fidelity in a nucleic acid synthesis reaction |
| US7544794B1 (en) | 2005-03-11 | 2009-06-09 | Steven Albert Benner | Method for sequencing DNA and RNA by synthesis |
| EP1987159B2 (en) * | 2006-02-08 | 2020-08-12 | Illumina Cambridge Limited | Method for sequencing a polynucleotide template |
| SG170802A1 (en) | 2006-03-31 | 2011-05-30 | Solexa Inc | Systems and devices for sequence by synthesis analysis |
| US8962293B2 (en) * | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| US8026091B2 (en) * | 2007-07-13 | 2011-09-27 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| US8652781B2 (en) | 2008-02-12 | 2014-02-18 | Pacific Biosciences Of California, Inc. | Cognate sampling kinetics |
| US8628940B2 (en) | 2008-09-24 | 2014-01-14 | Pacific Biosciences Of California, Inc. | Intermittent detection during analytical reactions |
| EP2271751B1 (en) | 2008-03-31 | 2015-07-22 | Pacific Biosciences of California, Inc. | Generation of modified polymerases for improved accuracy in single molecule sequencing |
| US8133672B2 (en) | 2008-03-31 | 2012-03-13 | Pacific Biosciences Of California, Inc. | Two slow-step polymerase enzyme systems and methods |
| EP2607496B1 (en) | 2008-12-23 | 2014-07-16 | Illumina, Inc. | Methods useful in nucleic acid sequencing protocols |
| WO2010111674A2 (en) | 2009-03-27 | 2010-09-30 | Life Technologies Corporation | Methods and apparatus for single molecule sequencing using energy transfer detection |
| WO2010141391A2 (en) | 2009-06-05 | 2010-12-09 | Life Technologies Corporation | Mutant dna polymerases |
| EP2742151B1 (en) | 2011-08-10 | 2017-10-25 | Life Technologies Corporation | Polymerase compositions |
| WO2013096692A1 (en) | 2011-12-21 | 2013-06-27 | Illumina, Inc. | Apparatus and methods for kinetic analysis and determination of nucleic acid sequences |
| TWI487789B (zh) | 2012-12-24 | 2015-06-11 | Ind Tech Res Inst | 單離的脫氧核糖核酸聚合酶、套組與其應用 |
| GB201301178D0 (en) | 2013-01-23 | 2013-03-06 | Dynamic Biosensors Gmbh | Method for sequencing a template nucleic acid immobilized on a substrate |
| EP3052652B1 (en) | 2013-09-30 | 2022-11-23 | Life Technologies Corporation | Polymerase compositions, methods of making and using same |
| US10077470B2 (en) | 2015-07-21 | 2018-09-18 | Omniome, Inc. | Nucleic acid sequencing methods and systems |
| US10597643B2 (en) | 2016-04-29 | 2020-03-24 | Omniome, Inc. | Polymerases engineered to reduce nucleotide-independent DNA binding |
| CN109154024B (zh) | 2016-04-29 | 2022-03-04 | 欧姆尼欧美公司 | 核酸序列测定方法 |
-
2017
- 2017-04-28 CN CN201780026228.XA patent/CN109154024B/zh not_active Expired - Fee Related
- 2017-04-28 SG SG11201809522WA patent/SG11201809522WA/en unknown
- 2017-04-28 CA CA3022431A patent/CA3022431C/en active Active
- 2017-04-28 JP JP2018556287A patent/JP6915939B2/ja not_active Expired - Fee Related
- 2017-04-28 AU AU2017258523A patent/AU2017258523B2/en not_active Ceased
- 2017-04-28 US US15/581,822 patent/US10584379B2/en active Active
- 2017-04-28 WO PCT/US2017/030135 patent/WO2017190012A1/en not_active Ceased
- 2017-04-28 KR KR1020187034503A patent/KR102231977B1/ko not_active Expired - Fee Related
- 2017-04-28 EP EP17722356.7A patent/EP3449011B1/en not_active Not-in-force
-
2020
- 2020-01-07 US US16/736,071 patent/US11976323B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| KR20190003660A (ko) | 2019-01-09 |
| CN109154024B (zh) | 2022-03-04 |
| EP3449011A1 (en) | 2019-03-06 |
| US20200199668A1 (en) | 2020-06-25 |
| JP2019526225A (ja) | 2019-09-19 |
| JP6915939B2 (ja) | 2021-08-11 |
| AU2017258523A1 (en) | 2018-11-22 |
| EP3449011B1 (en) | 2020-11-04 |
| WO2017190012A1 (en) | 2017-11-02 |
| AU2017258523B2 (en) | 2020-08-13 |
| US11976323B2 (en) | 2024-05-07 |
| SG11201809522WA (en) | 2018-11-29 |
| US20170314072A1 (en) | 2017-11-02 |
| KR102231977B1 (ko) | 2021-03-24 |
| CN109154024A (zh) | 2019-01-04 |
| CA3022431A1 (en) | 2017-11-02 |
| US10584379B2 (en) | 2020-03-10 |
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Legal Events
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