CA3016555A1 - Multiplexed immunohistochemistry using recombinant antibodies with epitope tags - Google Patents

Multiplexed immunohistochemistry using recombinant antibodies with epitope tags Download PDF

Info

Publication number
CA3016555A1
CA3016555A1 CA3016555A CA3016555A CA3016555A1 CA 3016555 A1 CA3016555 A1 CA 3016555A1 CA 3016555 A CA3016555 A CA 3016555A CA 3016555 A CA3016555 A CA 3016555A CA 3016555 A1 CA3016555 A1 CA 3016555A1
Authority
CA
Canada
Prior art keywords
epitope
seq
antibody
tag
tagged
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3016555A
Other languages
English (en)
French (fr)
Inventor
Michael Farrell
Eric May
Lei Tang
Wenjun Zhang
Antony HUBBARD
Tsu-Shuen Tsao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ventana Medical Systems Inc
Original Assignee
Ventana Medical Systems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ventana Medical Systems Inc filed Critical Ventana Medical Systems Inc
Publication of CA3016555A1 publication Critical patent/CA3016555A1/en
Pending legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6878Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids in epitope analysis
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2809Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2815Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD8
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2827Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against B7 molecules, e.g. CD80, CD86
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/40Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/40Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/42Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a HA(hemagglutinin)-tag
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2570/00Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Oncology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
CA3016555A 2016-03-08 2017-03-07 Multiplexed immunohistochemistry using recombinant antibodies with epitope tags Pending CA3016555A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201662305440P 2016-03-08 2016-03-08
US62/305,440 2016-03-08
US201662418667P 2016-11-07 2016-11-07
US62/418,667 2016-11-07
US201762461651P 2017-02-21 2017-02-21
US62/461,651 2017-02-21
PCT/US2017/021157 WO2017155996A1 (en) 2016-03-08 2017-03-07 Multiplexed immunohistochemistry using recombinant antibodies with epitope tags

Publications (1)

Publication Number Publication Date
CA3016555A1 true CA3016555A1 (en) 2017-09-14

Family

ID=58361148

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3016555A Pending CA3016555A1 (en) 2016-03-08 2017-03-07 Multiplexed immunohistochemistry using recombinant antibodies with epitope tags

Country Status (6)

Country Link
US (3) US11112413B2 (enExample)
EP (1) EP3426684B1 (enExample)
JP (3) JP6952706B2 (enExample)
AU (1) AU2017229370B2 (enExample)
CA (1) CA3016555A1 (enExample)
WO (1) WO2017155996A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3002674A1 (en) 2015-10-30 2017-05-04 Aleta Biotherapeutics Inc. Compositions and methods for treatment of cancer
MX2018005348A (es) 2015-10-30 2018-08-14 Aleta Biotherapeutics Inc Composiciones y metodos para transduccion de tumores.
AU2017229370B2 (en) * 2016-03-08 2021-03-11 Ventana Medical Systems, Inc. Multiplexed immunohistochemistry using recombinant antibodies with epitope tags
WO2019094095A1 (en) * 2017-11-07 2019-05-16 Trustees Of Tufts College Heteromultimeric binding molecules for toxin neutralization, compositions and methods thereof
CN113710365B (zh) * 2019-04-03 2023-12-19 万迈医疗仪器有限公司 用于在免疫测定中重复使用半抗原涂覆的探针的方法
CN110850094A (zh) * 2019-11-22 2020-02-28 西安交通大学 一种免疫组化双标单染试剂盒及其使用方法和应用
CN114113616B (zh) * 2021-12-07 2022-08-23 深圳裕策生物科技有限公司 一种试剂盒及其染色方法
WO2023162042A1 (ja) 2022-02-22 2023-08-31 日本電信電話株式会社 センサー付きハイドロゲル流路型デバイス
JP2025532064A (ja) 2022-09-22 2025-09-29 アジレント・テクノロジーズ・インク 免疫組織化学(ihc)プロトコールにおける使用のためおよびがんを診断するための抗ヒトicos抗体

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1564604A (en) 1925-06-12 1925-12-08 Chas S Hodges Water wheel
US5595707A (en) 1990-03-02 1997-01-21 Ventana Medical Systems, Inc. Automated biological reaction apparatus
US20030211630A1 (en) 1998-02-27 2003-11-13 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
AU763354B2 (en) 1998-02-27 2003-07-17 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US6582962B1 (en) 1998-02-27 2003-06-24 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US6670113B2 (en) 2001-03-30 2003-12-30 Nanoprobes Enzymatic deposition and alteration of metals
US7632652B2 (en) 2003-06-24 2009-12-15 Ventana Medical Systems, Inc. Enzyme-catalyzed metal deposition for the enhanced in situ detection of immunohistochemical epitopes and nucleic acid sequences
AU2006239315B2 (en) 2005-04-28 2012-03-01 Ventana Medical Systems, Inc. Enzymes conjugated to antiobodies via a PEG heterobifuctional linker
EP3095467B1 (en) 2005-11-23 2020-05-06 Ventana Medical Systems, Inc. Antibody-enzyme conjugate
DK2078197T3 (en) 2006-11-01 2016-05-23 Ventana Med Syst Inc Haptenes, hapten conjugates, compositions thereof and processes for their preparation and use
EP3561513A1 (en) 2007-05-23 2019-10-30 Ventana Medical Systems, Inc. Polymeric carriers for immunohistochemistry and in situ hybridization
US20080299555A1 (en) * 2007-05-30 2008-12-04 Hiroaki Nitta Multicolor chromogenic detection of biomarkers
WO2009111729A1 (en) * 2008-03-06 2009-09-11 Ventana Medical Systems, Inc. Anti-dnp antibodies and methods of use
CA2734783C (en) 2008-08-22 2016-04-19 Ventana Medical Systems, Inc. Method for chromogenic detection of two or more target molecules in a single sample
US20120034223A1 (en) * 2008-11-06 2012-02-09 Hall Christopher J Methods of improving the therapeutic efficacy and utility of antibody fragments
EP2993478B1 (en) 2009-10-19 2022-08-24 Ventana Medical Systems, Inc. Device and method for slide caching
WO2011139978A1 (en) 2010-05-04 2011-11-10 Ventana Medical Systems, Inc. Moving meniscus rinsing and mixing in cell staining
AU2011274369A1 (en) 2010-07-02 2012-12-06 Ventana Medical Systems, Inc. Hapten conjugates for target detection
CA3062786C (en) * 2010-07-09 2022-04-19 Janssen Vaccines & Prevention B.V. Anti-human respiratory syncytial virus (rsv) antibodies and methods of use
CA2940439C (en) 2014-02-24 2024-03-19 Ventana Medical Systems, Inc. Quinone methide analog signal amplification
EP4215898A1 (en) 2015-04-20 2023-07-26 Ventana Medical Systems, Inc. Inkjet deposition of reagents for histological samples
AU2017229370B2 (en) * 2016-03-08 2021-03-11 Ventana Medical Systems, Inc. Multiplexed immunohistochemistry using recombinant antibodies with epitope tags

Also Published As

Publication number Publication date
US20220057408A1 (en) 2022-02-24
JP6952706B2 (ja) 2021-10-20
AU2017229370B2 (en) 2021-03-11
AU2017229370A1 (en) 2018-08-16
WO2017155996A1 (en) 2017-09-14
US11112413B2 (en) 2021-09-07
JP7174126B2 (ja) 2022-11-17
US11988673B2 (en) 2024-05-21
US20240230667A1 (en) 2024-07-11
JP2023024990A (ja) 2023-02-21
EP3426684A1 (en) 2019-01-16
US20190094242A1 (en) 2019-03-28
JP2019512224A (ja) 2019-05-16
JP2022003064A (ja) 2022-01-11
EP3426684B1 (en) 2024-10-09

Similar Documents

Publication Publication Date Title
US11988673B2 (en) Multiplexed immunohistochemistry using recombinant antibodies with epitope tags
US20220178934A1 (en) Signaling conjugates and methods of use
AU2016357478B2 (en) Methods of identifying immune cells in PD-L1 positive tumor tissue
CA2900842A1 (en) Digitally enhanced microscopy for multiplexed histology
US20230151361A1 (en) Peptide nucleic acid conjugates
US20250321232A1 (en) Chromogenic multiplexing methods and systems for identifying a cancer of unknown primary origin
JP2024526175A (ja) 目的の分子を検出するための脱パラフィン細胞から出発する単一細胞elisaの使用

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20200102

EEER Examination request

Effective date: 20200102

EEER Examination request

Effective date: 20200102

EEER Examination request

Effective date: 20200102

EEER Examination request

Effective date: 20200102

EEER Examination request

Effective date: 20200102

EEER Examination request

Effective date: 20200102