CA3007066A1 - Methods for gender determination of avian embryos in unhatched eggs and means thereof - Google Patents

Methods for gender determination of avian embryos in unhatched eggs and means thereof Download PDF

Info

Publication number
CA3007066A1
CA3007066A1 CA3007066A CA3007066A CA3007066A1 CA 3007066 A1 CA3007066 A1 CA 3007066A1 CA 3007066 A CA3007066 A CA 3007066A CA 3007066 A CA3007066 A CA 3007066A CA 3007066 A1 CA3007066 A1 CA 3007066A1
Authority
CA
Canada
Prior art keywords
acid sequence
reporter gene
nucleic acid
seq
denoted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA3007066A
Other languages
English (en)
French (fr)
Inventor
Daniel Offen
Original Assignee
Eggxyt Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eggxyt Ltd filed Critical Eggxyt Ltd
Publication of CA3007066A1 publication Critical patent/CA3007066A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6879—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for sex determination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/30—Bird
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
CA3007066A 2015-12-03 2016-12-01 Methods for gender determination of avian embryos in unhatched eggs and means thereof Abandoned CA3007066A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562262409P 2015-12-03 2015-12-03
US62/262,409 2015-12-03
PCT/IL2016/051291 WO2017094015A1 (en) 2015-12-03 2016-12-01 Methods for gender determination of avian embryos in unhatched eggs and means thereof

Publications (1)

Publication Number Publication Date
CA3007066A1 true CA3007066A1 (en) 2017-06-08

Family

ID=58796444

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3007066A Abandoned CA3007066A1 (en) 2015-12-03 2016-12-01 Methods for gender determination of avian embryos in unhatched eggs and means thereof

Country Status (9)

Country Link
US (1) US20190029236A1 (enExample)
EP (1) EP3384051A4 (enExample)
JP (1) JP2019505175A (enExample)
KR (1) KR20180099704A (enExample)
CN (1) CN108474034A (enExample)
CA (1) CA3007066A1 (enExample)
HK (1) HK1257753A1 (enExample)
IL (2) IL259721B (enExample)
WO (1) WO2017094015A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107760715A (zh) * 2017-10-17 2018-03-06 云南瑞火生物科技有限公司 一种精准高效的转基因载体及其构建方法和应用
CN111315212A (zh) * 2017-09-19 2020-06-19 以色列农业和农村发展部农业研究组织(范卡尼中心) 经过基因组编辑的鸟

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MA48783A (fr) * 2017-05-25 2020-04-08 Eggxyt Ltd Procédés de détermination du sexe d'embryons aviaires dans les ufs non éclos et moyens correspondants
BR112019025358A2 (pt) * 2017-05-31 2020-07-07 Commonwealth Scientific And Industrial Research Organisation ovo aviário transgênico, ave transgênica, ovo ou progênie aviária, método para detectar um ovo aviário macho, método para produzir um ovo aviário, método para replicar um vírus, vírus produzidos pelo uso de ovo aviário, método para produzir uma composição de vacina, composição da vacina produzida pelo uso do método e método para produzir um ovo aviário transgênico ou uma ave produzida pelo ovo
US20200329685A1 (en) * 2018-01-04 2020-10-22 Ramot At Tel-Aviv University Ltd. Transgenic eukaryotic organisms and methods for gender selection
DE102018105512A1 (de) 2018-03-09 2019-09-12 Technische Universitaet Dresden Verfahren zur in-ovo fertilisations- und geschlechtsbestimmung am geschlossenen ei
EP3599465A1 (en) * 2018-07-24 2020-01-29 Tronico Method for determining a specific characteristic of an embryo in an unhatched egg
CN109402244B (zh) * 2018-12-20 2022-05-03 广西大学 一种哺乳动物胚胎性别鉴定方法
EA202192430A1 (ru) 2019-03-05 2022-02-11 Те Стейт Оф Израэл, Министри Оф Эгрикалчер Энд Рурал Дивелопмент, Эгрикалчарал Рисёрч Органайзейшн (Аро) (Волкани Сентер) Птицы с отредактированным геномом
CN111073963B (zh) * 2019-12-10 2023-04-14 南京农业大学 一种一日龄鉴别鸡受精蛋性别的方法
IL299499A (en) * 2020-06-26 2023-02-01 Sunnybrook Res Inst Yolk extract supplements for culture media and related methods
CN114793988B (zh) * 2022-04-19 2023-01-17 中国科学院海洋研究所 一种基于形态学鉴定孔鳐受精卵的方法
CN115960945B (zh) * 2022-12-05 2024-11-08 天津科技大学 蓝光诱导酿酒酵母定点dsb系统的构建

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06124456A (ja) 1992-10-09 1994-05-06 Sony Corp 光学ピックアップ装置
GB9511439D0 (en) 1995-06-06 1995-08-02 Isis Innovation Gene product and method
AUPO060996A0 (en) 1996-06-21 1996-07-18 University Of Melbourne, The Sexing gene
CA2264450A1 (en) * 1998-03-05 1999-09-05 Andras Nagy Methods for monitoring heterologous sex chromosomes
US6244214B1 (en) 1999-01-06 2001-06-12 Embrex, Inc. Concurrent in ovo injection and detection method and apparatus
GB0218955D0 (en) * 2002-08-14 2002-09-25 Roslin Inst Edinburgh Avian sex determination method
EP2376641A4 (en) * 2008-12-17 2013-01-09 Commw Scient Ind Res Org PROCEDURE FOR SEX MODULATION IN POULTRY
CA2751596A1 (en) * 2009-02-08 2010-08-12 The University Of Melbourne Sex-determination and methods of specifying same
WO2010103111A1 (en) 2009-03-13 2010-09-16 Van De Ven Beheer B.V. Method for avian sex determination
FR2969497B1 (fr) 2010-12-27 2013-06-28 Ceva Sante Animale Composition luminescente comme biomarqueur dans un oeuf aviaire, dispositif et procede correspondants.
KR102174868B1 (ko) * 2012-07-30 2020-11-06 인 오보 비.브이. 난 내 조류 배아의 성별, 생존능 및/또는 발달 단계 결정방법
DK2890973T3 (da) 2012-08-31 2020-11-30 Agri Advanced Tech Gmbh Spektrofotometrisk analyse af den embryonale kyllingefjerfarve
FR3023617B1 (fr) 2014-07-11 2018-06-15 Tronico Dispositif non invasif de determination de la fertilite et/ou du sexe d’un oeuf, et procede correspondant.

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111315212A (zh) * 2017-09-19 2020-06-19 以色列农业和农村发展部农业研究组织(范卡尼中心) 经过基因组编辑的鸟
CN107760715A (zh) * 2017-10-17 2018-03-06 云南瑞火生物科技有限公司 一种精准高效的转基因载体及其构建方法和应用
CN107760715B (zh) * 2017-10-17 2021-12-10 张业胜 一种转基因载体及其构建方法和应用

Also Published As

Publication number Publication date
EP3384051A4 (en) 2019-04-24
CN108474034A (zh) 2018-08-31
IL259721A (en) 2018-07-31
WO2017094015A1 (en) 2017-06-08
EP3384051A1 (en) 2018-10-10
KR20180099704A (ko) 2018-09-05
IL288409A (en) 2022-01-01
US20190029236A1 (en) 2019-01-31
IL259721B (en) 2021-12-01
JP2019505175A (ja) 2019-02-28
HK1257753A1 (zh) 2019-10-25

Similar Documents

Publication Publication Date Title
EP3384051A1 (en) Methods for gender determination of avian embryos in unhatched eggs and means thereof
CN105073981A (zh) 动物中性成熟的控制
EP3629719B1 (en) Methods for gender determination of avian embryos in unhatched eggs and means thereof
JP6004290B2 (ja) 不活化された内因性遺伝子座を有するトランスジェニックニワトリ
US20170223938A1 (en) Transgenic chickens with an inactivated endogenous gene locus
EP1206906A1 (en) Mouse having human cytochrome p450 transferred therein
JPWO2015199225A1 (ja) 家禽始原生殖細胞の遺伝子改変方法、遺伝子改変された家禽始原生殖細胞、遺伝子改変家禽の生産方法及び家禽卵
JPWO2005003342A1 (ja) メチル化を利用したトランスジェニック生物を作製する方法およびシステム
US20240052304A1 (en) Sterile avian embryos, production and uses thereof
US10858669B2 (en) Genetically modified chicken egg with an exogenous sequence knocked into the ovalbumin gene
US20200149063A1 (en) Methods for gender determination and selection of avian embryos in unhatched eggs
DK202170116A1 (en) Genetically modified salmon which produce sterile offspring
KR101724805B1 (ko) 유전자 적중 물질을 이용한 조류 유전자 편집방법 및 유전자 편집 조류
Idoko-Akoh Editing the genome of chicken primordial germ cells to introduce alleles and study gene function
OA19682A (en) Methods for gender determination of avian embryos in unhatched eggs and means thereof.

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20211115

FZDE Discontinued

Effective date: 20240422