CA2982304A1 - Method for generating aptamers with improved off-rates - Google Patents

Method for generating aptamers with improved off-rates Download PDF

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Publication number
CA2982304A1
CA2982304A1 CA2982304A CA2982304A CA2982304A1 CA 2982304 A1 CA2982304 A1 CA 2982304A1 CA 2982304 A CA2982304 A CA 2982304A CA 2982304 A CA2982304 A CA 2982304A CA 2982304 A1 CA2982304 A1 CA 2982304A1
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CA
Canada
Prior art keywords
target
aptamer
tissue
slow
rate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2982304A
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English (en)
French (fr)
Inventor
Dominic Zichi
Sheri K. Wilcox
Chris Bock
Daniel J. Schneider
Bruce Eaton
Larry Gold
Thale C. Jarvis
Jeffery D. Carter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Somalogic Inc
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Somalogic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Somalogic Inc filed Critical Somalogic Inc
Publication of CA2982304A1 publication Critical patent/CA2982304A1/en
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
CA2982304A 2009-07-09 2010-07-09 Method for generating aptamers with improved off-rates Abandoned CA2982304A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/499,967 2009-07-09
US12/499,967 US7964356B2 (en) 2007-01-16 2009-07-09 Method for generating aptamers with improved off-rates
CA2765857A CA2765857C (en) 2009-07-09 2010-07-09 Method for generating aptamers with improved off-rates

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2765857A Division CA2765857C (en) 2009-07-09 2010-07-09 Method for generating aptamers with improved off-rates

Publications (1)

Publication Number Publication Date
CA2982304A1 true CA2982304A1 (en) 2011-01-13

Family

ID=43429566

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2982304A Abandoned CA2982304A1 (en) 2009-07-09 2010-07-09 Method for generating aptamers with improved off-rates
CA2765857A Active CA2765857C (en) 2009-07-09 2010-07-09 Method for generating aptamers with improved off-rates

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2765857A Active CA2765857C (en) 2009-07-09 2010-07-09 Method for generating aptamers with improved off-rates

Country Status (7)

Country Link
US (1) US7964356B2 (enExample)
EP (1) EP2451981B1 (enExample)
JP (2) JP5926178B2 (enExample)
AU (1) AU2010271271B2 (enExample)
CA (2) CA2982304A1 (enExample)
ES (1) ES2610633T3 (enExample)
WO (1) WO2011006075A1 (enExample)

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Also Published As

Publication number Publication date
EP2451981A1 (en) 2012-05-16
EP2451981A4 (en) 2013-07-31
CA2765857C (en) 2018-03-06
AU2010271271B2 (en) 2015-01-29
JP2012532612A (ja) 2012-12-20
US20100055695A1 (en) 2010-03-04
AU2010271271A1 (en) 2012-01-19
CA2765857A1 (en) 2011-01-13
US7964356B2 (en) 2011-06-21
JP5926178B2 (ja) 2016-05-25
ES2610633T3 (es) 2017-04-28
WO2011006075A1 (en) 2011-01-13
JP2016065872A (ja) 2016-04-28
EP2451981B1 (en) 2016-10-19

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