AU2010271271B2 - Method for generating aptamers with improved off-rates - Google Patents

Method for generating aptamers with improved off-rates Download PDF

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Publication number
AU2010271271B2
AU2010271271B2 AU2010271271A AU2010271271A AU2010271271B2 AU 2010271271 B2 AU2010271271 B2 AU 2010271271B2 AU 2010271271 A AU2010271271 A AU 2010271271A AU 2010271271 A AU2010271271 A AU 2010271271A AU 2010271271 B2 AU2010271271 B2 AU 2010271271B2
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aptamer
target
tissue
minutes
slow
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AU2010271271A1 (en
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Chris Bock
Jeffrey D. Carter
Bruce Eaton
Larry Gold
Thale C. Jarvis
Daniel J. Schneider
Sheri K. Wilcox
Dominic Zichi
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Somalogic Inc
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Somalogic Inc
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Priority to AU2015255156A priority patent/AU2015255156C1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
AU2010271271A 2009-07-09 2010-07-09 Method for generating aptamers with improved off-rates Active AU2010271271B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2013203588A AU2013203588C1 (en) 2009-07-09 2013-04-11 Method for generating aptamers with improved off-rates
AU2015255156A AU2015255156C1 (en) 2009-07-09 2015-11-09 Method for generating aptamers with improved off-rates

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/499,967 2009-07-09
US12/499,967 US7964356B2 (en) 2007-01-16 2009-07-09 Method for generating aptamers with improved off-rates
PCT/US2010/041540 WO2011006075A1 (en) 2009-07-09 2010-07-09 Method for generating aptamers with improved off-rates

Related Child Applications (1)

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AU2013203588A Division AU2013203588C1 (en) 2009-07-09 2013-04-11 Method for generating aptamers with improved off-rates

Publications (2)

Publication Number Publication Date
AU2010271271A1 AU2010271271A1 (en) 2012-01-19
AU2010271271B2 true AU2010271271B2 (en) 2015-01-29

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US (1) US7964356B2 (enExample)
EP (1) EP2451981B1 (enExample)
JP (2) JP5926178B2 (enExample)
AU (1) AU2010271271B2 (enExample)
CA (2) CA2982304A1 (enExample)
ES (1) ES2610633T3 (enExample)
WO (1) WO2011006075A1 (enExample)

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US20100055695A1 (en) 2010-03-04
AU2010271271A1 (en) 2012-01-19
CA2765857A1 (en) 2011-01-13
US7964356B2 (en) 2011-06-21
JP5926178B2 (ja) 2016-05-25
ES2610633T3 (es) 2017-04-28
WO2011006075A1 (en) 2011-01-13
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EP2451981B1 (en) 2016-10-19
CA2982304A1 (en) 2011-01-13

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