CA2977546A1 - Generation and selection of embryos in vitro - Google Patents

Generation and selection of embryos in vitro Download PDF

Info

Publication number
CA2977546A1
CA2977546A1 CA2977546A CA2977546A CA2977546A1 CA 2977546 A1 CA2977546 A1 CA 2977546A1 CA 2977546 A CA2977546 A CA 2977546A CA 2977546 A CA2977546 A CA 2977546A CA 2977546 A1 CA2977546 A1 CA 2977546A1
Authority
CA
Canada
Prior art keywords
embryo
ovum
microfluidic device
isolation
sperm
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2977546A
Other languages
English (en)
French (fr)
Inventor
Kevin Chapman
Volker Kurz
Peggy RADEL
Grant Yonehiro
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bruker Cellular Analysis Inc
Original Assignee
Berkeley Lights Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Berkeley Lights Inc filed Critical Berkeley Lights Inc
Publication of CA2977546A1 publication Critical patent/CA2977546A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/07—Means for pre-treatment of biological substances by electrical or electromagnetic forces

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Electromagnetism (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physiology (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2977546A 2015-03-04 2016-03-04 Generation and selection of embryos in vitro Abandoned CA2977546A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562128458P 2015-03-04 2015-03-04
US62/128,458 2015-03-04
US15/061,295 US20160257918A1 (en) 2015-03-04 2016-03-04 Generation and Selection of Embryos in Vitro
US15/061,295 2016-03-04
PCT/US2016/021017 WO2016141343A1 (en) 2015-03-04 2016-03-04 Generation and selection of embryos in vitro

Publications (1)

Publication Number Publication Date
CA2977546A1 true CA2977546A1 (en) 2016-09-09

Family

ID=56849108

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2977546A Abandoned CA2977546A1 (en) 2015-03-04 2016-03-04 Generation and selection of embryos in vitro

Country Status (5)

Country Link
US (1) US20160257918A1 (https=)
JP (1) JP2018512116A (https=)
AU (1) AU2016226008A1 (https=)
CA (1) CA2977546A1 (https=)
WO (1) WO2016141343A1 (https=)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016094715A2 (en) 2014-12-10 2016-06-16 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
US10723988B2 (en) 2015-04-22 2020-07-28 Berkeley Lights, Inc. Microfluidic cell culture
US10799865B2 (en) 2015-10-27 2020-10-13 Berkeley Lights, Inc. Microfluidic apparatus having an optimized electrowetting surface and related systems and methods
EP3380238A1 (en) 2015-11-23 2018-10-03 Berkeley Lights, Inc. In situ-generated microfluidic isolation structures, kits and methods of use thereof
CN109922885B (zh) 2016-03-16 2022-05-10 伯克利之光生命科技公司 用于基因组编辑克隆的选择和传代的方法、系统和装置
IL263274B2 (en) 2016-05-26 2023-10-01 Berkeley Lights Inc Covalently adapted surfaces, kits and methods for their production and uses
CN110268108B (zh) 2016-12-12 2022-09-06 埃克切拉生物科学公司 用于使用微毛细管阵列进行筛选的方法和系统
JP6561036B2 (ja) * 2016-12-14 2019-08-14 憲隆 福永 胚培養装置およびその撮像装置
EP3562796B1 (en) 2016-12-30 2023-04-19 Xcella Biosciences, Inc. Multi-stage sample recovery system
JPWO2018207454A1 (ja) * 2017-05-12 2020-03-19 株式会社ニコン 対象物操作装置、及び対象物操作方法
KR20200026878A (ko) 2017-06-06 2020-03-11 지머젠 인코포레이티드 균류 균주를 개량하기 위한 htp 게놈 공학 플랫폼
CN107723237B (zh) * 2017-11-23 2023-12-08 北京大学深圳医院 辅助生殖试验用多用途培养皿
US11491483B2 (en) 2018-02-15 2022-11-08 Ohio State Innovation Foundation Microfluidic devices and methods for high throughput electroporation
BR112020024839A2 (pt) 2018-06-06 2021-05-18 Zymergen Inc. manipulação de genes envolvidos em transdução de sinal para controlar a morfologia fúngica durante a fermentação e produção
ES3039910T3 (en) 2018-12-06 2025-10-27 Xcella Biosciences Inc Lateral loading of microcapillary arrays
WO2020168258A1 (en) * 2019-02-15 2020-08-20 Berkeley Lights, Inc. Laser-assisted repositioning of a micro-object and culturing of an attachment-dependent cell in a microfluidic environment
WO2020223555A1 (en) 2019-04-30 2020-11-05 Berkeley Lights, Inc. Methods for encapsulating and assaying cells
JP2019170404A (ja) * 2019-07-22 2019-10-10 憲隆 福永 胚培養装置およびその撮像装置
CN114467112B (zh) * 2019-09-06 2025-04-15 布里格姆妇女医院 用于辅助生殖程序的质量保证度量的自动评估
KR102403774B1 (ko) 2020-06-23 2022-05-31 의료법인 성광의료재단 자궁 내막을 모사하기 위한 3차원 생체 모사 칩 및 이를 이용한 자궁 내막 모사 방법
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
JP2021006066A (ja) * 2020-10-26 2021-01-21 憲隆 福永 胚培養装置およびその撮像装置
US20220135920A1 (en) * 2020-11-02 2022-05-05 Reprohealth Technologies, Inc. Gas permeable intravaginal culture device
DE102020214862B4 (de) * 2020-11-26 2022-08-04 Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen, Körperschaft des öffentlichen Rechts System und Vorrichtung zur Charakterisierung von Veränderungen des Reifungsvorgangs einer Eizelle, sowie deren Verwendung
GB202019628D0 (en) * 2020-12-11 2021-01-27 Vivoplex Group Ltd A cell culture system controller
US12377415B2 (en) 2020-12-30 2025-08-05 The Trustees Of The University Of Pennsylvania Photoactivated selective release (PHASR) of droplets from microwell arrays
CN116710547A (zh) * 2021-01-20 2023-09-05 医疗法人浅田女性诊所 胚胎培养装置及其拍摄装置
WO2025074135A1 (en) * 2023-10-06 2025-04-10 Sorbonne Universite (Su) A microfluidic chip, apparatus and method for in vitro fertilization
WO2025074134A1 (en) * 2023-10-06 2025-04-10 Sorbonne Universite (Su) Microstructure and method for capturing and manipulating a target in a fluid medium

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5627066A (en) * 1991-05-14 1997-05-06 Mount Sinai School Of Medicine Of The City University Of New York Method and apparatus for in vitro fertilization
JPH0734698B2 (ja) * 1992-06-02 1995-04-19 株式会社機能性ペプチド研究所 牛胚の体外発生方法
CA2480728A1 (en) * 2002-04-01 2003-10-16 Fluidigm Corporation Microfluidic particle-analysis systems
US7763462B2 (en) * 2006-06-16 2010-07-27 The Board Of Trustees Of The Leland Stanford Junior University BDNF facilitation of oocyte, zygote and pre-implantation embryo maturation
US7985579B2 (en) * 2007-11-19 2011-07-26 Genx International, Inc. Specimen culturing assembly suitable for use in in-vitro fertilization and in other cell culturing procedures
CN102449163A (zh) * 2009-04-03 2012-05-09 加利福尼亚大学董事会 分选细胞和其它生物微粒的方法和装置
US8753498B2 (en) * 2009-06-25 2014-06-17 Purdue Research Foundation Open optoelectrowetting droplet actuation device and method
CN101947124B (zh) * 2010-06-25 2012-07-04 博奥生物有限公司 一种集成式微流控芯片装置及其使用方法
WO2012072822A1 (en) * 2010-12-03 2012-06-07 Mindseeds Laboratories Srl Microanalysis of cellular function
WO2013056002A1 (en) * 2011-10-12 2013-04-18 University Of Iowa Research Foundation Use of microrna for assessing embryos grown in vitro and improving culture media
US9050593B2 (en) * 2011-11-23 2015-06-09 Wisconsin Alumni Research Foundation Self-loading microfluidic device and methods of use
WO2013096864A1 (en) * 2011-12-21 2013-06-27 The Regents Of The University Of California Non-invasive methods for assessing oocyte quality for in vitro fertilization
US9857333B2 (en) * 2012-10-31 2018-01-02 Berkeley Lights, Inc. Pens for biological micro-objects

Also Published As

Publication number Publication date
US20160257918A1 (en) 2016-09-08
WO2016141343A1 (en) 2016-09-09
AU2016226008A1 (en) 2017-10-26
JP2018512116A (ja) 2018-05-17

Similar Documents

Publication Publication Date Title
US20160257918A1 (en) Generation and Selection of Embryos in Vitro
US20230023831A1 (en) In-situ generated microfluidic assay structures, related kits, and methods of use thereof
US10829728B2 (en) Microfluidic devices for optically-driven convection and displacement, kits and methods thereof
US20210069698A1 (en) Microfluidic reporter cell assay methods and kits thereof
JP6196614B2 (ja) 運動性細胞の分析及び選別
EP3230718A1 (en) Automated detection of assay-positive areas in microfluidic devices
KR20210089193A (ko) 미세유체 디바이스에서 생물학적 세포를 분석하기 위한 방법
WO2017181135A2 (en) Methods, systems and kits for in-pen assays
CN102449163A (zh) 分选细胞和其它生物微粒的方法和装置
EP3270801A1 (en) Generation and selection of embryos in vitro
US20250027941A1 (en) Methods of assaying micro-objects in a microfluidic device
WO2025072196A1 (en) Methods for culturing and assaying b cell lymphocytes

Legal Events

Date Code Title Description
FZDE Discontinued

Effective date: 20220525