CA2969038C - Methods, systems, and apparatuses for quantitative analysis of heterogeneous biomarker distribution - Google Patents

Methods, systems, and apparatuses for quantitative analysis of heterogeneous biomarker distribution Download PDF

Info

Publication number
CA2969038C
CA2969038C CA2969038A CA2969038A CA2969038C CA 2969038 C CA2969038 C CA 2969038C CA 2969038 A CA2969038 A CA 2969038A CA 2969038 A CA2969038 A CA 2969038A CA 2969038 C CA2969038 C CA 2969038C
Authority
CA
Canada
Prior art keywords
image
data
fov
spectral
tumor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2969038A
Other languages
English (en)
French (fr)
Other versions
CA2969038A1 (en
Inventor
Michael Barnes
David Chafin
Karl E. Garsha
Thomas M. Grogan
Esteban ROBERTS
Benjamin C. Stevens
Frank R. Ventura
Christophe CHEFD´HOTEL
Kandavel Shanmugam
Joe Gray
Damien RAMUNNO-JOHNSON
Tothu Vu (Tania)
Brian Jay Druker
Thomas Jacob
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oregon Health and Science University
Ventana Medical Systems Inc
Original Assignee
Oregon Health and Science University
Ventana Medical Systems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oregon Health and Science University, Ventana Medical Systems Inc filed Critical Oregon Health and Science University
Publication of CA2969038A1 publication Critical patent/CA2969038A1/en
Application granted granted Critical
Publication of CA2969038C publication Critical patent/CA2969038C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06F—ELECTRIC DIGITAL DATA PROCESSING
    • G06F18/00—Pattern recognition
    • G06F18/20—Analysing
    • G06F18/21—Design or setup of recognition systems or techniques; Extraction of features in feature space; Blind source separation
    • G06F18/213—Feature extraction, e.g. by transforming the feature space; Summarisation; Mappings, e.g. subspace methods
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/11—Region-based segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/90—Determination of colour characteristics
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/695—Preprocessing, e.g. image segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/698—Matching; Classification
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06F—ELECTRIC DIGITAL DATA PROCESSING
    • G06F18/00—Pattern recognition
    • G06F18/20—Analysing
    • G06F18/23—Clustering techniques
    • G06F18/232—Non-hierarchical techniques
    • G06F18/2321—Non-hierarchical techniques using statistics or function optimisation, e.g. modelling of probability density functions
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06F—ELECTRIC DIGITAL DATA PROCESSING
    • G06F18/00—Pattern recognition
    • G06F18/20—Analysing
    • G06F18/23—Clustering techniques
    • G06F18/232—Non-hierarchical techniques
    • G06F18/2321—Non-hierarchical techniques using statistics or function optimisation, e.g. modelling of probability density functions
    • G06F18/23211—Non-hierarchical techniques using statistics or function optimisation, e.g. modelling of probability density functions with adaptive number of clusters
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10032—Satellite or aerial image; Remote sensing
    • G06T2207/10036—Multispectral image; Hyperspectral image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10064—Fluorescence image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20024—Filtering details
    • G06T2207/20032—Median filtering
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20092—Interactive image processing based on input by user
    • G06T2207/20104—Interactive definition of region of interest [ROI]
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20112—Image segmentation details
    • G06T2207/20152—Watershed segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30096—Tumor; Lesion
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V10/00—Arrangements for image or video recognition or understanding
    • G06V10/20—Image preprocessing
    • G06V10/25—Determination of region of interest [ROI] or a volume of interest [VOI]
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V10/00—Arrangements for image or video recognition or understanding
    • G06V10/40—Extraction of image or video features
    • G06V10/56—Extraction of image or video features relating to colour

Landscapes

  • Engineering & Computer Science (AREA)
  • Theoretical Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Multimedia (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medical Informatics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Quality & Reliability (AREA)
  • Radiology & Medical Imaging (AREA)
  • Data Mining & Analysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Artificial Intelligence (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Evolutionary Computation (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CA2969038A 2014-12-03 2015-12-03 Methods, systems, and apparatuses for quantitative analysis of heterogeneous biomarker distribution Active CA2969038C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462086840P 2014-12-03 2014-12-03
US62/086,840 2014-12-03
PCT/EP2015/078532 WO2016087589A1 (en) 2014-12-03 2015-12-03 Methods, systems, and apparatuses for quantitative analysis of heterogeneous biomarker distribution

Publications (2)

Publication Number Publication Date
CA2969038A1 CA2969038A1 (en) 2016-06-09
CA2969038C true CA2969038C (en) 2023-07-04

Family

ID=54979630

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2969038A Active CA2969038C (en) 2014-12-03 2015-12-03 Methods, systems, and apparatuses for quantitative analysis of heterogeneous biomarker distribution

Country Status (7)

Country Link
US (4) US10664967B2 (https=)
EP (1) EP3227830B8 (https=)
JP (1) JP6654634B2 (https=)
CN (1) CN107209934B (https=)
AU (1) AU2015357088A1 (https=)
CA (1) CA2969038C (https=)
WO (1) WO2016087589A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210327059A1 (en) * 2018-08-07 2021-10-21 Deep Bio Inc. Diagnosis result generation system and method
US20210406591A1 (en) * 2019-08-29 2021-12-30 Tencent Technology (Shenzhen) Company Limited Medical image processing method and apparatus, and medical image recognition method and apparatus

Families Citing this family (112)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013055995A2 (en) 2011-10-14 2013-04-18 President And Fellows Of Harvard College Sequencing by structure assembly
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
EP3578666A1 (en) 2013-03-12 2019-12-11 President and Fellows of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
WO2015069827A2 (en) * 2013-11-06 2015-05-14 H. Lee Moffitt Cancer Center And Research Institute, Inc. Pathology case review, analysis and prediction
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
JP6893877B2 (ja) 2014-10-29 2021-06-23 スペクトラル エムディー, インコーポレイテッドSpectral Md, Inc. 組織を分類するための反射モードマルチスペクトル時間分解型光学イメージングの方法および装置
US20180040120A1 (en) * 2014-12-29 2018-02-08 Flagship Biosciences, Inc. Methods for quantitative assessment of mononuclear cells in muscle tissue sections
AU2015373423A1 (en) * 2014-12-30 2017-05-18 Ventana Medical Systems, Inc. Systems and methods for co-expression analysis in immunoscore computation
AU2016214922B2 (en) 2015-02-02 2019-07-25 Stryker European Operations Limited Methods and systems for characterizing tissue of a subject
EP3256850B1 (en) 2015-02-09 2025-07-09 Slingshot Biosciences, Inc. Hydrogel particles with tunable optical properties and methods for using the same
WO2017051229A1 (en) 2015-09-23 2017-03-30 Novadaq Technologies Inc. Methods and systems for management of data derived from medical imaging
JP6882282B2 (ja) 2015-11-03 2021-06-02 プレジデント アンド フェローズ オブ ハーバード カレッジ 三次元核酸含有マトリックスの立体撮像のための方法と装置
US9524205B1 (en) * 2016-01-04 2016-12-20 International Business Machines Corporation Code fingerprint-based processor malfunction detection
EP3203413A1 (en) * 2016-02-08 2017-08-09 Ecole Polytechnique Federale De Lausanne (Epfl) Method for quantitative measurement of a biomarker by in situ immunofluorescence and uses thereof
CN109415761B (zh) 2016-04-25 2022-09-20 哈佛学院董事及会员团体 用于原位分子检测的杂交链反应方法
WO2017216123A1 (en) * 2016-06-13 2017-12-21 Nanolive Sa Method of characterizing and imaging microscopic objects
US10890576B2 (en) * 2016-06-23 2021-01-12 Konica Minolta, Inc. Image processing device, image processing method, and recording medium
US10572996B2 (en) * 2016-06-28 2020-02-25 Contextvision Ab Method and system for detecting pathological anomalies in a digital pathology image and method for annotating a tissue slide
EP3275361B1 (en) * 2016-07-29 2024-06-05 Leica Instruments (Singapore) Pte. Ltd. Medical imaging apparatus and method for the imaging of a light-sensitive object, such as biological tissue
EP3490454A4 (en) * 2016-07-29 2020-08-05 Novadaq Technologies ULC METHODS AND SYSTEMS FOR CHARACTERIZING A SUBJECT FABRIC USING MACHINE LEARNING
CN109923216B (zh) 2016-08-31 2024-08-02 哈佛学院董事及会员团体 将生物分子的检测组合到使用荧光原位测序的单个试验的方法
WO2018052912A1 (en) * 2016-09-13 2018-03-22 Oregon Health & Science University Pathogen quantification using labeled probes
TR201619702A1 (tr) * 2016-12-27 2018-07-23 Univ Yeditepe Hücresel otomatlar i̇le kümeleme problemi̇ni̇n isi yayilimi tabanli çözülmesi̇ne yöneli̇k bi̇r yöntem
CN110573066A (zh) 2017-03-02 2019-12-13 光谱Md公司 用于多光谱截肢部位分析的机器学习系统和技术
US9738937B1 (en) * 2017-03-31 2017-08-22 Cellmax, Ltd. Identifying candidate cells using image analysis
CN110785654A (zh) * 2017-06-28 2020-02-11 文塔纳医疗系统公司 系统级校准
CN107818341A (zh) * 2017-10-25 2018-03-20 天津大学 一种基于改进K‑means算法的颜色提取方法
EP3721406B1 (en) * 2017-12-05 2024-01-24 Ventana Medical Systems, Inc. Method of computing tumor spatial and inter-marker heterogeneity
US11568657B2 (en) 2017-12-06 2023-01-31 Ventana Medical Systems, Inc. Method of storing and retrieving digital pathology analysis results
WO2019110561A1 (en) * 2017-12-06 2019-06-13 Ventana Medical Systems, Inc. Method of storing and retrieving digital pathology analysis results
CN108228900B (zh) * 2018-02-06 2021-12-24 国网山西省电力公司电力科学研究院 基于分层结构的电力设备多光谱数据中心模型建立方法
CN108427657B (zh) * 2018-02-28 2021-05-25 东北大学 一种地下水封油库水幕系统有效性分析与调控方法
CN108537785B (zh) * 2018-04-04 2021-04-30 北京大学人民医院 一种子宫超声微蠕动视频处理方法
GB201806002D0 (en) * 2018-04-11 2018-05-23 Univ Liverpool Methods of spectroscopic analysis
JP2021515240A (ja) * 2018-04-12 2021-06-17 グーグル エルエルシーＧｏｏｇｌｅ ＬＬＣ 定量的バイオマーカデータのオーバレイを有する病理学用拡張現実顕微鏡
CN108664937A (zh) * 2018-05-14 2018-10-16 宁波江丰生物信息技术有限公司 一种基于数字病理切片扫描仪的多区域扫描方法
WO2019219651A1 (en) 2018-05-15 2019-11-21 Ventana Medical Systems, Inc. Quantitation of signal in stain aggregates
CN108648199B (zh) * 2018-05-21 2022-07-19 上海工程技术大学 基于分水岭和cv模型的超声相控阵ndt图像分割方法
WO2019231298A1 (ko) * 2018-05-31 2019-12-05 (주) 솔 영상 기반의 대면적 시료 분석 장치, 매질 특성 차이를 이용한 영상 기반의 시료 분석 장치 및 이를 이용하여 시료를 측정하고 분석하는 방법
CN108562750A (zh) * 2018-06-01 2018-09-21 安图实验仪器(郑州)有限公司 印迹膜条结果判读方法
KR102162895B1 (ko) * 2018-06-04 2020-10-07 주식회사 딥바이오 듀얼 클래스를 지원하는 질병 진단 시스템 및 그 방법
EP3591574A3 (en) * 2018-07-06 2020-01-15 Universität Zürich Method and computer program for clustering large multiplexed spatially resolved data of a biological sample
JP2021531790A (ja) 2018-07-27 2021-11-25 ベンタナ メディカル システムズ， インコーポレイテッド 自動化された原位置ハイブリッド形成分析のためのシステム
EP3608701A1 (de) * 2018-08-09 2020-02-12 Olympus Soft Imaging Solutions GmbH Verfahren zur bereitstellung wenigstens einer auswertemethode für proben
WO2020066042A1 (ja) 2018-09-28 2020-04-02 オリンパス株式会社 顕微鏡システム、投影ユニット、及び、画像投影方法
JPWO2020066043A1 (ja) * 2018-09-28 2021-08-30 オリンパス株式会社 顕微鏡システム、投影ユニット、及び、画像投影方法
EP3988986B1 (en) 2018-09-28 2025-11-19 Evident Corporation Microscope system
EP3988987A4 (en) 2018-09-28 2023-09-20 Evident Corporation MICROSCOPE SYSTEM, PROJECTION UNIT AND IMAGE PROJECTION METHOD
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
WO2020078974A1 (en) 2018-10-17 2020-04-23 Koninklijke Philips N.V. Mapping image signatures of cancer cells to genetic signatures
KR102812782B1 (ko) * 2018-12-14 2025-05-23 스펙트랄 엠디, 인크. 고정밀도 다중-애퍼쳐 스펙트럼 이미징을 위한 시스템 및 방법
US10740884B2 (en) * 2018-12-14 2020-08-11 Spectral Md, Inc. System and method for high precision multi-aperture spectral imaging
KR20240163190A (ko) 2018-12-14 2024-11-18 스펙트랄 엠디, 인크. 상처들의 평가, 치유 예측 및 치료를 위한 머신 학습 시스템들 및 방법들
US12327298B2 (en) * 2018-12-31 2025-06-10 Oregon Health & Science University Translation of images of stained biological material
CN109712142B (zh) * 2019-01-11 2023-03-28 山东省计算中心（国家超级计算济南中心） 基于染色分离的乳腺癌Ki67/ER/PR核染色细胞计数方法
KR102805539B1 (ko) * 2019-02-01 2025-05-12 한국전자통신연구원 인물 영역 추출 방법, 이를 이용하는 영상 처리 장치 및 인물 영역 추출 시스템
CN110163250B (zh) * 2019-04-10 2023-10-24 创新先进技术有限公司 基于分布式调度的图像脱敏处理系统、方法以及装置
CN109961448A (zh) * 2019-04-10 2019-07-02 杭州智团信息技术有限公司 组织病变区域勾勒方法及系统
CN113950701B (zh) * 2019-04-12 2025-07-08 因芬尼昂影像公司 用于检测组织中的术中造影剂的成像系统
US12050830B2 (en) * 2019-04-25 2024-07-30 Dolby Laboratories Licensing Corporation Content-aware PQ range analyzer and tone mapping in live feeds
US12152969B2 (en) * 2019-06-04 2024-11-26 JelloX Biotech Inc. Method for preparation of tissue sections
US20200388032A1 (en) * 2019-06-04 2020-12-10 JelloX Biotech Inc. Three dimensional histopathology imaging method and system thereof
CN110208244B (zh) * 2019-06-18 2020-07-28 华南理工大学 一种基于拉曼光谱测试果蔬组织中细胞水平水分含量和分布的方法
US20210027890A1 (en) * 2019-07-24 2021-01-28 ConnSante BioTech, Inc. Detecting, evaluating and predicting system for cancer risk
CA3153886A1 (en) * 2019-10-18 2021-04-22 Nanostring Technologies, Inc. Systems and methods for spatial mapping of expression profiling
AU2020388047A1 (en) * 2019-11-18 2022-06-23 10X Genomics, Inc. Systems and methods for tissue classification
JP2021081342A (ja) * 2019-11-20 2021-05-27 ソニーグループ株式会社 情報処理システムおよび情報処理装置
WO2021102039A1 (en) * 2019-11-21 2021-05-27 10X Genomics, Inc, Spatial analysis of analytes
EP4435718A3 (en) * 2019-11-22 2024-12-18 10X Genomics, Inc. Systems and methods for spatial analysis of analytes using fiducial alignment
US20210182531A1 (en) * 2019-12-17 2021-06-17 Applied Materials, Inc. Registration techniques for multiplexed fluorescence in-situ hybridization images
US11977723B2 (en) * 2019-12-17 2024-05-07 Palantir Technologies Inc. Image tiling and distributive modification
KR102512332B1 (ko) * 2019-12-24 2023-03-22 광운대학교 산학협력단 신경세포의 신호전달 학습이 가능한 바이오닉 뉴럴 인공지능 프로세서
US11908130B2 (en) * 2020-01-03 2024-02-20 Protein Metrics Llc Apparatuses and methods for digital pathology
US11494907B2 (en) * 2020-01-06 2022-11-08 PAIGE.AI, Inc. Systems and methods for processing electronic images for computational assessment of disease
JP7759328B2 (ja) 2020-01-24 2025-10-23 スリングショット バイオサイエンシーズ， インコーポレイテッド 細胞様較正粒子のための組成物および方法
WO2021155063A1 (en) 2020-01-29 2021-08-05 Readcoor, Llc Compositions and methods for analyte detection
JP7592737B2 (ja) * 2020-03-06 2024-12-02 ボストンジーン コーポレイション 多重免疫蛍光イメージングを使用する組織特性の決定
CZ2020157A3 (cs) * 2020-03-20 2021-11-10 Univerzita Hradec Králové Způsob zpracování předzpracovaného obrazu a zařízení k provádění tohoto způsobu
US20230081277A1 (en) * 2020-04-27 2023-03-16 Ventana Medical Systems, Inc. Methods for efficiently determining density and spatial relationship of multiple cell types in regions of tissue
CA3177834A1 (en) 2020-05-04 2021-11-11 Jeffrey Kim Compositions and methods for passive optical barcoding for multiplexed assays
KR102512331B1 (ko) * 2020-05-06 2023-03-22 광운대학교 산학협력단 신경세포의 신호 전달 체계를 이용하여 비정형적 연산의 출력이 가능한 바이오닉 뉴럴 인공지능 프로세서
GB2594939B (en) * 2020-05-11 2022-05-11 Forsite Diagnostics Ltd Assay reading method
FR3112010B1 (fr) * 2020-06-26 2022-07-08 Institut National De Rech En Informatique Et En Automatique Dispositif d’imagerie tumorale
US11422355B2 (en) * 2020-07-22 2022-08-23 Sartorius Bioanalytical Instruments, Inc. Method and system for acquisition of fluorescence images of live-cell biological samples
US11308616B2 (en) 2020-08-04 2022-04-19 PAIGE.AI, Inc. Systems and methods to process electronic images to provide image-based cell group targeting
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
CN111982638B (zh) * 2020-08-20 2023-11-10 诒福生物科技南通有限公司 生物组织染色方法、装置、计算机设备和存储介质
US12092569B2 (en) 2020-10-21 2024-09-17 Green Vision Systems Ltd. Method and system for detecting a biological substance by hyperspectral imaging
GB2628478A (en) * 2020-10-29 2024-09-25 Ambergen Inc Novel photocleavable mass-tags for multiplexed mass spectrometric imaging of tissues using biomolecular probes
EP4697281A3 (en) 2020-12-11 2026-04-22 Enumera Molecular, Inc. Methods and systems for image processing
CN112911105B (zh) * 2021-01-19 2022-03-29 中国计量科学研究院 一种数字pcr结果读取装置及读取方法
WO2022212413A1 (en) 2021-03-30 2022-10-06 Spectral Md, Inc. System and method for high precision snapshot multi-spectral imaging based on multiplexed illumination
US11734242B1 (en) * 2021-03-31 2023-08-22 Amazon Technologies, Inc. Architecture for resolution of inconsistent item identifiers in a global catalog
CN113297723B (zh) * 2021-04-22 2022-11-08 哈尔滨理工大学 基于均值漂移-灰色关联分析的电主轴温度测点优化方法
US11995810B2 (en) * 2021-04-27 2024-05-28 City University Of Hong Kong System and method for generating a stained image
CN113240685B (zh) * 2021-04-29 2023-06-13 平安科技（深圳）有限公司 图像层次化超像素分割方法、系统、电子设备及存储介质
CN113450877B (zh) * 2021-06-28 2022-04-08 深圳裕泰抗原科技有限公司 一种基于多重免疫组化技术的生物标志物分析方法及其应用
CN113593640B (zh) * 2021-08-03 2023-07-28 哈尔滨市米杰生物科技有限公司 一种鳞癌组织功能状态与细胞组分评估方法及系统
WO2023049209A1 (en) * 2021-09-22 2023-03-30 Volastra Therapeutics, Inc. Systems and methods for evaluation of mitotic events using machine-learning
US12327625B2 (en) * 2021-09-24 2025-06-10 PAIGE.AI, Inc. Systems and methods to process electronic images to identify tumor subclones and relationships among subclones
CN113762416B (zh) * 2021-10-15 2023-05-30 南京澄实生物科技有限公司 基于多模态深度编码的抗原免疫原性预测方法和系统
JP2024539956A (ja) 2021-10-29 2024-10-31 スリングショット バイオサイエンシーズ， インコーポレイテッド フィーダー細胞としておよび合成抗原提示細胞としてのヒドロゲル粒子
TWI813145B (zh) * 2022-01-25 2023-08-21 佛教慈濟醫療財團法人 多重螢光組織影像分析方法及其系統
CN119907921A (zh) 2022-05-05 2025-04-29 弹弓生物科学公司 用于血液学的作为红细胞模拟物的工程化颗粒和含有工程化颗粒的组合物
AU2023280490A1 (en) 2022-06-02 2024-12-05 Slingshot Biosciences, Inc. Apoptotic cell mimic
CN115099287B (zh) * 2022-08-24 2022-11-11 山东大学 基于图傅里叶变换的空间可变基因识别与分析系统
EP4608872A2 (en) 2022-10-26 2025-09-03 Slingshot Biosciences, Inc. Size-tunable synthetic particles with tunable optical properties and methods for using the same for immune cell activation
CN120239886A (zh) * 2022-12-30 2025-07-01 深圳华大生命科学研究院 一种组织切片数据异质性的评估方法以及相关设备
WO2024185434A1 (ja) * 2023-03-03 2024-09-12 ソニーグループ株式会社 情報処理装置、生体試料解析システム及び生体試料解析方法
WO2025049609A1 (en) 2023-08-29 2025-03-06 Slingshot Biosciences, Inc. Cd34 stem cell mimics
CN120927952B (zh) * 2025-09-30 2025-12-05 南通大学附属医院 一种基于空间重构的多重免疫荧光检测方法、设备及介质

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101916359B (zh) * 2005-01-27 2012-06-20 剑桥研究和仪器设备股份有限公司 把样本的不同区域分类到相应类别的方法和设备
BRPI0610115B8 (pt) * 2005-05-13 2021-07-27 Tripath Imaging Inc método de coloração de uma amostra para formação de imagem microscópica, método de colorir artificialmente uma amostra, método de obtenção de medidas de uma amostra de uma imagem dela, método de selecionar uma região de interesse em uma lâmina, método de determinação de dados de densidade óptica e método de separação de cromógenos para a imagem de amostra biológica colorida
CN101553579B (zh) 2006-10-06 2016-01-20 赛里根有限公司 用于定向生物标志物信号放大的荧光方法和材料
EP2104420A2 (en) 2006-10-30 2009-09-30 George Mason Intellectual Properties, Inc. Tissue preservation and fixation method
WO2008063378A2 (en) 2006-11-01 2008-05-29 Ventana Medical Systems, Inc. Haptens, hapten conjugates, compositions thereof and method for their preparation and use
US8244021B2 (en) 2006-12-20 2012-08-14 Ventana Medical Systems, Inc. Quantitative, multispectral image analysis of tissue specimens stained with quantum dots
WO2010033508A1 (en) 2008-09-16 2010-03-25 Historx, Inc. Reproducible quantification of biomarker expression
CA2745961A1 (en) * 2008-12-04 2010-06-10 The Regents Of The University Of California Materials and methods for determining diagnosis and prognosis of prostate cancer
US10126216B2 (en) 2011-02-17 2018-11-13 Ventana Medical Systems, Inc. Method for tissue sample fixation
CN108267581A (zh) * 2012-08-24 2018-07-10 耶鲁大学 用于高通量多重检测的系统、装置和方法
JP2014110592A (ja) * 2012-12-04 2014-06-12 Toshiba Corp 画像処理装置
US8995740B2 (en) * 2013-04-17 2015-03-31 General Electric Company System and method for multiplexed biomarker quantitation using single cell segmentation on sequentially stained tissue

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210327059A1 (en) * 2018-08-07 2021-10-21 Deep Bio Inc. Diagnosis result generation system and method
US11995819B2 (en) * 2018-08-07 2024-05-28 Deep Bio Inc. Diagnosis result generation system and method
US20210406591A1 (en) * 2019-08-29 2021-12-30 Tencent Technology (Shenzhen) Company Limited Medical image processing method and apparatus, and medical image recognition method and apparatus
US12229224B2 (en) * 2019-08-29 2025-02-18 Tencent Technology (Shenzhen) Company Limited Medical image processing method and apparatus, and medical image recognition method and apparatus

Also Published As

Publication number Publication date
US11049247B2 (en) 2021-06-29
US20210279870A1 (en) 2021-09-09
AU2015357088A1 (en) 2017-05-18
US12579642B2 (en) 2026-03-17
WO2016087589A1 (en) 2016-06-09
JP6654634B2 (ja) 2020-02-26
US20170262984A1 (en) 2017-09-14
EP3227830B1 (en) 2020-05-20
EP3227830B8 (en) 2020-07-01
EP3227830A1 (en) 2017-10-11
JP2018509591A (ja) 2018-04-05
US20200219256A1 (en) 2020-07-09
CA2969038A1 (en) 2016-06-09
US10664967B2 (en) 2020-05-26
CN107209934A (zh) 2017-09-26
CN107209934B (zh) 2021-03-30
US20230260116A1 (en) 2023-08-17
US11663717B2 (en) 2023-05-30

Similar Documents

Publication Publication Date Title
US12579642B2 (en) Methods, systems, and apparatuses for quantitative analysis of heterogeneous biomarker distribution
US12293517B2 (en) Systems for cell shape estimation
JP7273215B2 (ja) 画像処理のための自動アッセイ評価および正規化
US12367692B2 (en) Systems for automated in situ hybridization analysis
CN111448584B (zh) 计算肿瘤空间和标记间异质性的方法
JP6800152B2 (ja) 組織学画像中の核の分類
Mori et al. Characterizing the tumor immune microenvironment with tyramide-based multiplex immunofluorescence
CN111417958A (zh) 用于生物图像中的联合细胞和区域分类的深度学习系统和方法
JP2021506003A (ja) デジタル病理学分析結果の格納および読み出し方法
OsunkoyaAdeboye et al. Deep UV microscopy identifies prostatic basal cells: an important biomarker for prostate cancer diagnostics
Maiques et al. Recent advances in tissue imaging for cancer research
Pompey et al. Quantitative fluorescence co-localization to study protein–receptor complexes
Roberts et al. Mapping cancer signaling networks by an integrated multiplexed tissue imaging platform
Grote et al. Exploring the spatial dimension of estrogen and progesterone signaling: detection of nuclear labeling in lobular epithelial cells in normal mammary glands adjacent to breast cancer
Garsha et al. Multi-modal contrast of tissue anatomy enables correlative biomarker imaging
Stokes et al. Quality Control and Analysis Algorithms for Tissue Microarrays as Biomarker Validation Tools
Kung Assessing a hyperspectral image analysis system to study tumor and immune cell spatial organizations within lung tumor microenvironments

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20200611

MPN Maintenance fee for patent paid

Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 9TH ANNIV.) - STANDARD

Year of fee payment: 9

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20241129

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT DETERMINED COMPLIANT

Effective date: 20250110

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20250110

MPN Maintenance fee for patent paid

Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 10TH ANNIV.) - STANDARD

Year of fee payment: 10

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20251119

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20251119