CA2958042C - Method for detecting target molecule, and kit for use in said method - Google Patents

Method for detecting target molecule, and kit for use in said method Download PDF

Info

Publication number
CA2958042C
CA2958042C CA2958042A CA2958042A CA2958042C CA 2958042 C CA2958042 C CA 2958042C CA 2958042 A CA2958042 A CA 2958042A CA 2958042 A CA2958042 A CA 2958042A CA 2958042 C CA2958042 C CA 2958042C
Authority
CA
Canada
Prior art keywords
target molecule
antibody
detection
antibodies
enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2958042A
Other languages
English (en)
French (fr)
Other versions
CA2958042A1 (en
Inventor
Hiroyuki Noji
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japan Science and Technology Agency
Original Assignee
Japan Science and Technology Agency
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Science and Technology Agency filed Critical Japan Science and Technology Agency
Publication of CA2958042A1 publication Critical patent/CA2958042A1/en
Application granted granted Critical
Publication of CA2958042C publication Critical patent/CA2958042C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531Production of immunochemical test materials
    • G01N33/532Production of labelled immunochemicals
    • G01N33/535Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2958042A 2014-09-22 2015-09-09 Method for detecting target molecule, and kit for use in said method Active CA2958042C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2014192311 2014-09-22
JP2014-192311 2014-09-22
PCT/JP2015/004577 WO2016047068A1 (ja) 2014-09-22 2015-09-09 ターゲット分子の検出方法及びこれに用いられるキット

Publications (2)

Publication Number Publication Date
CA2958042A1 CA2958042A1 (en) 2016-03-31
CA2958042C true CA2958042C (en) 2022-05-31

Family

ID=55580619

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2958042A Active CA2958042C (en) 2014-09-22 2015-09-09 Method for detecting target molecule, and kit for use in said method

Country Status (12)

Country Link
US (1) US20170227538A1 (ru)
EP (1) EP3199948B1 (ru)
JP (2) JP6522636B2 (ru)
KR (1) KR102461615B1 (ru)
CN (1) CN107076740B (ru)
AU (1) AU2015323168B2 (ru)
BR (1) BR112017005252B1 (ru)
CA (1) CA2958042C (ru)
ES (1) ES2792873T3 (ru)
RU (2) RU2020129667A (ru)
SG (1) SG11201701312TA (ru)
WO (1) WO2016047068A1 (ru)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017200070A1 (ja) 2016-05-19 2017-11-23 凸版印刷株式会社 標的分子の検出方法及び標的分子検出キット
JP7454945B2 (ja) 2017-07-03 2024-03-25 アボット・ラボラトリーズ 血液中のユビキチンカルボキシ末端ヒドロラーゼl1レベルを測定するための、改善された方法
JP7344801B2 (ja) 2017-12-09 2023-09-14 アボット・ラボラトリーズ グリア原線維性酸性タンパク質(gfap)及び/又はユビキチンカルボキシ末端ヒドロラーゼl1(uch-l1)を使用する、整形外科損傷を負っており、軽度外傷性脳損傷(tbi)などの頭部への損傷を負ったか又は負った可能性がある対象についての診断及び査定の一助となるための方法
BR112020010085A2 (pt) 2017-12-09 2020-10-13 Abbott Laboratories métodos para auxiliar no diagnóstico e avaliar uma lesão cerebral traumática em um indivíduo humano usando uma combinação de gfap e uch-l1
JP7437303B2 (ja) 2017-12-29 2024-02-22 アボット・ラボラトリーズ 外傷性脳損傷を診断及び査定するための、新規のバイオマーカー及び方法
CN110865060A (zh) * 2019-11-29 2020-03-06 中国人民大学 一种“一步法”检测甘胆酸的方法
CN112816706B (zh) * 2021-01-06 2023-08-29 上海理工大学 一种数字elisa系统及其使用方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3296077B2 (ja) 1994-03-29 2002-06-24 株式会社ニコン 酵素免疫検査法
KR100575407B1 (ko) * 1998-01-09 2006-05-03 미네소타 마이닝 앤드 매뉴팩춰링 캄파니 효소 특이적 절단성 폴리뉴클레오티드 기질 및 이의 분석방법
US6383740B2 (en) * 1999-07-30 2002-05-07 Bioergonomics, Inc. Methods for simultaneously detecting both members of a binding pair
CA2545006C (en) * 2003-12-12 2013-09-17 Saint Louis University Biosensors for detecting macromolecules and other analytes
GB0517447D0 (en) * 2005-08-25 2005-10-05 Smart Holograms Ltd Use of holographic sensor
JP2009000673A (ja) * 2007-05-24 2009-01-08 Metawater Co Ltd 浄水プロセスの監視装置及び監視方法
US8067192B2 (en) * 2007-06-05 2011-11-29 City Of Hope Methods for detection of botulinum neurotoxin
US20100330592A1 (en) * 2008-01-29 2010-12-30 Key Marc E Method for detecting truncated molecules
AU2012226920B2 (en) 2011-03-08 2015-02-19 Japan Science And Technology Agency Bead sealing method, method for detecting target molecule, array, kit, and target molecule detection device
EP2954102B1 (en) * 2013-02-08 2018-12-19 Bio-Rad Laboratories, Inc. Affinity-based partition assay for detection of target molecules

Also Published As

Publication number Publication date
AU2015323168B2 (en) 2020-07-02
AU2015323168A1 (en) 2017-03-09
EP3199948A1 (en) 2017-08-02
JP6522636B2 (ja) 2019-05-29
JPWO2016047068A1 (ja) 2017-07-06
JP2019144264A (ja) 2019-08-29
ES2792873T3 (es) 2020-11-12
US20170227538A1 (en) 2017-08-10
KR20170057241A (ko) 2017-05-24
RU2017107871A (ru) 2018-09-10
SG11201701312TA (en) 2017-04-27
EP3199948B1 (en) 2020-03-11
RU2017107871A3 (ru) 2019-03-07
CN107076740B (zh) 2020-06-02
BR112017005252A2 (pt) 2018-08-07
CA2958042A1 (en) 2016-03-31
KR102461615B1 (ko) 2022-11-01
WO2016047068A1 (ja) 2016-03-31
CN107076740A (zh) 2017-08-18
RU2020129667A (ru) 2020-10-08
EP3199948A4 (en) 2018-04-11
BR112017005252B1 (pt) 2023-12-26

Similar Documents

Publication Publication Date Title
CA2958042C (en) Method for detecting target molecule, and kit for use in said method
Yang et al. Aptamer fluorescence signal recovery screening for multiplex mycotoxins in cereal samples based on photonic crystal microsphere suspension array
KR101029343B1 (ko) 면역분석 기반의 항원 검출용 키트 및 항원 검출 방법
US9678068B2 (en) Ultra-sensitive detection of molecules using dual detection methods
AU2012308326B2 (en) Molecular diagnostic assay device and method of use
CA2734029C (en) Methods for determining the concentration of an analyte in solution
Akama et al. Wash-and amplification-free digital immunoassay based on single-particle motion analysis
US7704730B2 (en) Multiplexed assay methods
US20070259381A1 (en) Methods and arrays for target analyte detection and determination of reaction components that affect a reaction
US8029985B2 (en) Amplified bioassay
US20160123969A1 (en) Methods, materials, and kits for covalently associating molecular species with a surface of an object
KR101338136B1 (ko) 금 나노입자를 이용하여 신호 증폭도가 향상된, 표적 물질의 다중화 처리 분석법과 그 시스템
Long et al. Compact quantitative optic fiber-based immunoarray biosensor for rapid detection of small analytes
JP7463486B2 (ja) シグナル発生型デジタルアッセイにおけるノイズを低減する方法
US20220205992A1 (en) Materials and kits relating to association of reporter species and targeting entities with beads
KR101359379B1 (ko) Cmos 이미지 센서의 픽셀 분석을 이용한 고감도 바이오센서
CN113564158B (zh) 一种DNA引物、包括该引物的CRISPR/Cas12a系统及试剂盒
JP2024079779A (ja) シグナル発生型デジタルアッセイにおけるノイズを低減する方法

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20200623