CA2948488A1 - Expansion of definitive endoderm lineage cells - Google Patents

Expansion of definitive endoderm lineage cells Download PDF

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Publication number
CA2948488A1
CA2948488A1 CA2948488A CA2948488A CA2948488A1 CA 2948488 A1 CA2948488 A1 CA 2948488A1 CA 2948488 A CA2948488 A CA 2948488A CA 2948488 A CA2948488 A CA 2948488A CA 2948488 A1 CA2948488 A1 CA 2948488A1
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CA
Canada
Prior art keywords
cells
mrna
homo sapiens
protein
prod
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2948488A
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English (en)
French (fr)
Inventor
Alireza Rezania
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Janssen Biotech Inc
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Janssen Biotech Inc
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Filing date
Publication date
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of CA2948488A1 publication Critical patent/CA2948488A1/en
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0607Non-embryonic pluripotent stem cells, e.g. MASC
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/02Atmosphere, e.g. low oxygen conditions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/105Insulin-like growth factors [IGF]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/16Activin; Inhibin; Mullerian inhibiting substance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40Regulators of development
    • C12N2501/415Wnt; Frizzeled
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70Enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/90Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CA2948488A 2008-04-24 2009-04-22 Expansion of definitive endoderm lineage cells Abandoned CA2948488A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/108,872 2008-04-24
US12/108,872 US7939322B2 (en) 2008-04-24 2008-04-24 Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm
CA2722619A CA2722619C (en) 2008-04-24 2009-04-22 Pluripotent cells

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2722619A Division CA2722619C (en) 2008-04-24 2009-04-22 Pluripotent cells

Publications (1)

Publication Number Publication Date
CA2948488A1 true CA2948488A1 (en) 2009-10-29

Family

ID=41129142

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2948488A Abandoned CA2948488A1 (en) 2008-04-24 2009-04-22 Expansion of definitive endoderm lineage cells
CA2722619A Active CA2722619C (en) 2008-04-24 2009-04-22 Pluripotent cells

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2722619A Active CA2722619C (en) 2008-04-24 2009-04-22 Pluripotent cells

Country Status (14)

Country Link
US (2) US7939322B2 (enExample)
EP (3) EP2669366B1 (enExample)
JP (1) JP5763524B2 (enExample)
KR (3) KR102057056B1 (enExample)
CN (1) CN102083965B (enExample)
AU (1) AU2009239442B2 (enExample)
BR (1) BRPI0911599A2 (enExample)
CA (2) CA2948488A1 (enExample)
ES (2) ES2633455T3 (enExample)
MX (1) MX2010011739A (enExample)
PL (2) PL2283113T3 (enExample)
RU (1) RU2553538C2 (enExample)
WO (1) WO2009132063A2 (enExample)
ZA (1) ZA201008391B (enExample)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090203141A1 (en) * 2003-05-15 2009-08-13 Shi-Lung Lin Generation of tumor-free embryonic stem-like pluripotent cells using inducible recombinant RNA agents
US8017395B2 (en) 2004-12-17 2011-09-13 Lifescan, Inc. Seeding cells on porous supports
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
CA2695225C (en) 2007-07-31 2021-06-01 Lifescan, Inc. Differentiation of human embryonic stem cells to pancreatic endocrine
US9062290B2 (en) 2007-11-27 2015-06-23 Lifescan, Inc. Differentiation of human embryonic stem cells
BRPI0908033A2 (pt) 2008-02-21 2015-08-04 Centocor Ortho Biotech Inc Método placas de superfície modificada e composições para adesão, cultura e desprendimento de célula
BRPI0914116A2 (pt) 2008-06-30 2019-09-17 Centocor Ortho Biotech Inc diferenciação de células-tronco pluripotentes
BRPI0919883A2 (pt) 2008-10-31 2015-08-11 Centocor Ortho Biotech Inc Diferenciação de células-tronco embrionárias humanas para a linhagem endócrina pancreática
EP2346988B1 (en) 2008-10-31 2017-05-31 Janssen Biotech, Inc. Differentiation of human embryonic stem cells to the pancreatic endocrine lineage
ES2642070T3 (es) 2008-11-20 2017-11-15 Janssen Biotech, Inc. Cultivo de células madre pluripotentes en microportadores
CN102257132B (zh) 2008-11-20 2014-09-03 森托科尔奥索生物科技公司 用于在平面基底上进行细胞附着和培养的方法和组合物
BR112012001480A2 (pt) 2009-07-20 2015-09-01 Janssen Biotech Inc Diferenciação de células-tronco embriônicas humanas
EP2516626B1 (en) * 2009-12-23 2017-05-10 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
WO2011079017A2 (en) 2009-12-23 2011-06-30 Centocor Ortho Biotech Inc. Differentiation of human embryonic stem cells
WO2011109279A2 (en) 2010-03-01 2011-09-09 Centocor Ortho Biotech Inc. Methods for purifying cells derived from pluripotent stem cells
EP3498825A1 (en) 2010-05-12 2019-06-19 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
US9506036B2 (en) 2010-08-31 2016-11-29 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
RU2673946C1 (ru) 2010-08-31 2018-12-03 Янссен Байотек, Инк. Дифференцирование плюрипотентных стволовых клеток
ES2659393T3 (es) 2010-08-31 2018-03-15 Janssen Biotech, Inc. Diferenciación de células madre embrionarias humanas
JP6312591B2 (ja) 2011-06-21 2018-04-18 ノヴォ ノルディスク アー/エス 多能性幹細胞からの胚体内胚葉の効率的な誘導
EP2794857A4 (en) 2011-12-22 2015-07-08 Janssen Biotech Inc DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS IN SINGLE INSULIN POSITIVE CELLS
BR112014020183A8 (pt) * 2012-02-14 2017-07-11 Washington State Univ Research Foundation Método de manutenção e enriquecimento de células-tronco espermatogoniais (sscs), método enriquecimento de células-tronco espermatogoniais (sscs), meio para manutenção e enriquecimento de células-tronco espermatogoniais bovinas, população de células-tronco espermatogoniais enriquecidas, método para gerar pelo menos um descendente mamífero
US10106773B2 (en) * 2012-03-01 2018-10-23 University Of Miami Isolation and use of pluripotent stem cell population from adult neural crest-derived tissues
HK1206058A1 (en) * 2012-03-07 2015-12-31 Janssen Biotech, Inc. Defined media for expansion and maintenance of pluripotent stem cells
EP2837681B1 (en) * 2012-04-06 2018-02-21 Kyoto University Method for inducing erythropoietin-producing cell
CN104284977A (zh) * 2012-05-07 2015-01-14 詹森生物科技公司 人胚胎干细胞向胰腺内胚层的分化
ES2897649T3 (es) 2012-06-08 2022-03-02 Janssen Biotech Inc Diferenciación de células madre embrionarias humanas en células endocrinas pancreáticas
KR20150030709A (ko) * 2012-06-14 2015-03-20 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 췌장 내분비 세포로의 분화
GB2504996A (en) 2012-08-17 2014-02-19 Univ Keele Embryonic stem cell culture method
JP6470687B2 (ja) 2012-09-03 2019-02-13 ノヴォ ノルディスク アー/エス 小分子を用いた多能性幹細胞からの膵臓内胚葉の作製
EP4219683A1 (en) 2012-12-31 2023-08-02 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endocrine cells using hb9 regulators
AU2013368221B2 (en) 2012-12-31 2018-11-01 Janssen Biotech, Inc. Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells
RU2687379C2 (ru) 2012-12-31 2019-05-13 Янссен Байотек, Инк. Суспендирование и кластеризация плюрипотентных клеток человека с целью их дифференцировки в панкреатические эндокринные клетки
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
EP2981605B1 (en) * 2013-04-03 2019-06-19 FUJIFILM Cellular Dynamics, Inc. Methods and compositions for culturing endoderm progenitor cells in suspension
GB201317869D0 (en) * 2013-10-09 2013-11-20 Cambridge Entpr Ltd In vitro production of foregut stem cells
CA2949056C (en) 2014-05-16 2025-10-07 Janssen Biotech, Inc. USE OF SMALL MOLECULES TO IMPROVE MAFA GENE EXPRESSION IN PANCREATIC ENDOCRINE CELLS
US10494608B2 (en) 2015-04-24 2019-12-03 University Of Copenhagen Isolation of bona fide pancreatic progenitor cells
MA45479A (fr) 2016-04-14 2019-02-20 Janssen Biotech Inc Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen
NL2017670B1 (en) * 2016-10-25 2018-05-04 Stichting Het Nederlands Kanker Inst Antoni Van Leeuwenhoek Ziekenhuis Molecular indicators for predicting response to hormonal therapy in breast cancer
CN118924958A (zh) 2016-11-10 2024-11-12 韦尔赛特公司 含有pdx1胰腺内胚层细胞的细胞递送装置及其方法
US10767164B2 (en) 2017-03-30 2020-09-08 The Research Foundation For The State University Of New York Microenvironments for self-assembly of islet organoids from stem cells differentiation
US10391156B2 (en) 2017-07-12 2019-08-27 Viacyte, Inc. University donor cells and related methods
ES2988722T3 (es) * 2017-07-17 2024-11-21 Miltenyi Biotec Bv & Co Kg Un método para el perfilado de la expresión de proteínas de una sola célula de células progenitoras dopaminérgicas mesencefálicas de la placa del suelo
CA2983845C (en) 2017-10-26 2024-01-30 University Of Copenhagen Generation of glucose-responsive beta cells
US12378572B2 (en) 2018-09-07 2025-08-05 Crispr Therapeutics Ag Universal donor cells
WO2020243666A1 (en) 2019-05-31 2020-12-03 W. L. Gore & Associates, Inc. A biocompatible membrane composite
EP3976237A1 (en) 2019-05-31 2022-04-06 W.L. Gore & Associates Inc. Cell encapsulation devices with controlled oxygen diffusion distances
EP3976236A1 (en) 2019-05-31 2022-04-06 W.L. Gore & Associates Inc. A biocompatible membrane composite
AU2020283056B2 (en) 2019-05-31 2023-06-08 Viacyte, Inc. A biocompatible membrane composite
WO2021044377A1 (en) 2019-09-05 2021-03-11 Crispr Therapeutics Ag Universal donor cells
US11118196B2 (en) 2019-09-05 2021-09-14 Crispr Therapeutics Ag Universal donor cells
WO2022144856A1 (en) 2020-12-31 2022-07-07 Crispr Therapeutics Ag Universal donor cells

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US583308A (en) * 1897-05-25 m miehle
US5834308A (en) 1994-04-28 1998-11-10 University Of Florida Research Foundation, Inc. In vitro growth of functional islets of Langerhans
US5843780A (en) * 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
JP3880795B2 (ja) 1997-10-23 2007-02-14 ジェロン・コーポレーション フィーダー細胞を含まない培養物中で、霊長類由来始原幹細胞を増殖させるための方法
US6667176B1 (en) * 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US6610540B1 (en) 1998-11-18 2003-08-26 California Institute Of Technology Low oxygen culturing of central nervous system progenitor cells
EP1144597A2 (en) * 1999-01-21 2001-10-17 Vitro Diagnostics, Inc. Immortalized cell lines and methods of making the same
US20030082155A1 (en) * 1999-12-06 2003-05-01 Habener Joel F. Stem cells of the islets of langerhans and their use in treating diabetes mellitus
AU778155B2 (en) * 1999-12-13 2004-11-18 Scripps Research Institute, The Markers for identification and isolation of pancreatic islet alpha and beta cell progenitors
US7439064B2 (en) * 2000-03-09 2008-10-21 Wicell Research Institute, Inc. Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
US7005252B1 (en) * 2000-03-09 2006-02-28 Wisconsin Alumni Research Foundation Serum free cultivation of primate embryonic stem cells
US6458589B1 (en) 2000-04-27 2002-10-01 Geron Corporation Hepatocyte lineage cells derived from pluripotent stem cells
US7432104B2 (en) * 2001-08-06 2008-10-07 Bresgen Inc. Methods for the culture of human embryonic stem cells on human feeder cells
KR101008868B1 (ko) * 2001-12-07 2011-01-17 제론 코포레이션 인간 배아 줄기세포 유래의 섬세포
US20060003446A1 (en) 2002-05-17 2006-01-05 Gordon Keller Mesoderm and definitive endoderm cell populations
AU2003273573A1 (en) 2002-05-28 2003-12-19 Becton, Dickinson And Company Expansion and transdifferentiation of human acinar cells
US6877147B2 (en) * 2002-07-22 2005-04-05 Broadcom Corporation Technique to assess timing delay by use of layout quality analyzer comparison
EP1539930A4 (en) 2002-07-29 2006-08-09 Es Cell Int Pte Ltd METHOD IN MULTIPLE STAGES OF DIFFERENTIATION OF POSITIVE INSULIN-SENSITIVE CELLS, GLUCOSE
US20060194315A1 (en) 2003-03-31 2006-08-31 Condie Brian G Compositions and methods for the control, differentiaton and/or manipulation of pluripotent cells through a gamma-secretase signaling pathway
ITRM20030395A1 (it) 2003-08-12 2005-02-13 Istituto Naz Per Le Malattie Infettive Lazz Terreno di coltura per il mantenimento, la proliferazione e il differenziamento di cellule di mammifero.
CN103898045B (zh) * 2003-12-23 2019-02-01 维亚希特公司 定形内胚层
US20050266554A1 (en) * 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
US20050233446A1 (en) * 2003-12-31 2005-10-20 Parsons Xuejun H Defined media for stem cell culture
CN1950498A (zh) 2004-03-10 2007-04-18 加利福尼亚大学董事会 培养胚胎干细胞的组合物和方法
KR101395516B1 (ko) 2004-04-27 2014-05-14 비아싸이트, 인크. Pdx1 발현 내배엽
AU2005272078B2 (en) 2004-07-09 2011-04-14 Viacyte, Inc. Methods for identifying factors for differentiating definitive endoderm
US8187878B2 (en) 2004-08-13 2012-05-29 University Of Georgia Research Foundation, Inc. Methods for increasing definitive endoderm differentiation of pluripotent human embryonic stem cells with PI-3 kinase inhibitors
JP5265204B2 (ja) 2005-03-04 2013-08-14 ライフスキャン・インコーポレイテッド 成人膵臓由来間質細胞
EP1943334A1 (en) 2005-09-02 2008-07-16 Agency for Science, Technology and Research Method of deriving progenitor cell line
AU2006292021B2 (en) 2005-09-12 2012-05-31 Es Cell International Pte Ltd. Cardiomyocyte production
WO2007082963A1 (es) 2006-01-18 2007-07-26 Fundación Instituto Valenciano De Infertilidad Líneas de células madre embrionarias humanas y métodos para usar las mismas
EP2650359B1 (en) 2006-03-02 2022-05-04 Viacyte, Inc. Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production
US20070259423A1 (en) * 2006-05-02 2007-11-08 Jon Odorico Method of differentiating stem cells into cells of the endoderm and pancreatic lineage
WO2007139929A2 (en) 2006-05-25 2007-12-06 The Burnham Institute For Medical Research Methods for culture and production of single cell populations of human embryonic stem cells
US20080003676A1 (en) 2006-06-26 2008-01-03 Millipore Corporation Growth of embryonic stem cells
ES2537578T3 (es) 2007-07-18 2015-06-09 Lifescan, Inc. Diferenciación de las células madre de embriones humanos
CA2695225C (en) * 2007-07-31 2021-06-01 Lifescan, Inc. Differentiation of human embryonic stem cells to pancreatic endocrine
US8623648B2 (en) * 2008-04-24 2014-01-07 Janssen Biotech, Inc. Treatment of pluripotent cells

Also Published As

Publication number Publication date
KR20100134127A (ko) 2010-12-22
MX2010011739A (es) 2010-11-22
BRPI0911599A2 (pt) 2020-08-18
EP2669366B1 (en) 2018-01-31
ES2663421T3 (es) 2018-04-12
ZA201008391B (en) 2014-04-30
EP2669366A3 (en) 2014-03-05
KR101832902B1 (ko) 2018-02-27
ES2633455T3 (es) 2017-09-21
PL2669366T3 (pl) 2018-06-29
US7939322B2 (en) 2011-05-10
CN102083965B (zh) 2014-10-15
CA2722619C (en) 2016-12-20
AU2009239442A1 (en) 2009-10-29
USRE43876E1 (en) 2012-12-25
JP2011518562A (ja) 2011-06-30
CN102083965A (zh) 2011-06-01
EP3327114A1 (en) 2018-05-30
EP2283113A2 (en) 2011-02-16
KR20160150656A (ko) 2016-12-30
EP2283113B1 (en) 2017-05-17
KR102057056B1 (ko) 2019-12-18
RU2010147817A (ru) 2012-05-27
US20090269845A1 (en) 2009-10-29
KR101690773B1 (ko) 2016-12-28
CA2722619A1 (en) 2009-10-29
WO2009132063A2 (en) 2009-10-29
EP2669366A2 (en) 2013-12-04
PL2283113T3 (pl) 2017-09-29
WO2009132063A3 (en) 2010-03-18
JP5763524B2 (ja) 2015-08-12
AU2009239442B2 (en) 2015-06-11
EP3327114B1 (en) 2019-11-06
KR20180021250A (ko) 2018-02-28
RU2553538C2 (ru) 2015-06-20

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Effective date: 20161115

FZDE Discontinued

Effective date: 20210831

FZDE Discontinued

Effective date: 20210831