CA2948488A1 - Expansion of definitive endoderm lineage cells - Google Patents

Expansion of definitive endoderm lineage cells Download PDF

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Publication number
CA2948488A1
CA2948488A1 CA2948488A CA2948488A CA2948488A1 CA 2948488 A1 CA2948488 A1 CA 2948488A1 CA 2948488 A CA2948488 A CA 2948488A CA 2948488 A CA2948488 A CA 2948488A CA 2948488 A1 CA2948488 A1 CA 2948488A1
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Prior art keywords
cells
mrna
homo sapiens
protein
prod
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Abandoned
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CA2948488A
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English (en)
French (fr)
Inventor
Alireza Rezania
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Janssen Biotech Inc
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Janssen Biotech Inc
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Filing date
Publication date
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of CA2948488A1 publication Critical patent/CA2948488A1/en
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0607Non-embryonic pluripotent stem cells, e.g. MASC
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/02Atmosphere, e.g. low oxygen conditions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/105Insulin-like growth factors [IGF]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/16Activin; Inhibin; Mullerian inhibiting substance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40Regulators of development
    • C12N2501/415Wnt; Frizzeled
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70Enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/90Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CA2948488A 2008-04-24 2009-04-22 Expansion of definitive endoderm lineage cells Abandoned CA2948488A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/108,872 2008-04-24
US12/108,872 US7939322B2 (en) 2008-04-24 2008-04-24 Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm
CA2722619A CA2722619C (en) 2008-04-24 2009-04-22 Pluripotent cells

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2722619A Division CA2722619C (en) 2008-04-24 2009-04-22 Pluripotent cells

Publications (1)

Publication Number Publication Date
CA2948488A1 true CA2948488A1 (en) 2009-10-29

Family

ID=41129142

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2948488A Abandoned CA2948488A1 (en) 2008-04-24 2009-04-22 Expansion of definitive endoderm lineage cells
CA2722619A Active CA2722619C (en) 2008-04-24 2009-04-22 Pluripotent cells

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2722619A Active CA2722619C (en) 2008-04-24 2009-04-22 Pluripotent cells

Country Status (14)

Country Link
US (2) US7939322B2 (cg-RX-API-DMAC7.html)
EP (3) EP2669366B1 (cg-RX-API-DMAC7.html)
JP (1) JP5763524B2 (cg-RX-API-DMAC7.html)
KR (3) KR101690773B1 (cg-RX-API-DMAC7.html)
CN (1) CN102083965B (cg-RX-API-DMAC7.html)
AU (1) AU2009239442B2 (cg-RX-API-DMAC7.html)
BR (1) BRPI0911599A2 (cg-RX-API-DMAC7.html)
CA (2) CA2948488A1 (cg-RX-API-DMAC7.html)
ES (2) ES2633455T3 (cg-RX-API-DMAC7.html)
MX (1) MX2010011739A (cg-RX-API-DMAC7.html)
PL (2) PL2283113T3 (cg-RX-API-DMAC7.html)
RU (1) RU2553538C2 (cg-RX-API-DMAC7.html)
WO (1) WO2009132063A2 (cg-RX-API-DMAC7.html)
ZA (1) ZA201008391B (cg-RX-API-DMAC7.html)

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Also Published As

Publication number Publication date
KR102057056B1 (ko) 2019-12-18
EP2669366B1 (en) 2018-01-31
JP2011518562A (ja) 2011-06-30
KR20100134127A (ko) 2010-12-22
AU2009239442A1 (en) 2009-10-29
ZA201008391B (en) 2014-04-30
JP5763524B2 (ja) 2015-08-12
MX2010011739A (es) 2010-11-22
KR20160150656A (ko) 2016-12-30
US20090269845A1 (en) 2009-10-29
EP3327114B1 (en) 2019-11-06
PL2669366T3 (pl) 2018-06-29
EP2283113B1 (en) 2017-05-17
CA2722619C (en) 2016-12-20
EP2283113A2 (en) 2011-02-16
AU2009239442B2 (en) 2015-06-11
ES2633455T3 (es) 2017-09-21
KR101832902B1 (ko) 2018-02-27
EP2669366A3 (en) 2014-03-05
ES2663421T3 (es) 2018-04-12
WO2009132063A2 (en) 2009-10-29
RU2010147817A (ru) 2012-05-27
CA2722619A1 (en) 2009-10-29
WO2009132063A3 (en) 2010-03-18
PL2283113T3 (pl) 2017-09-29
EP3327114A1 (en) 2018-05-30
KR20180021250A (ko) 2018-02-28
CN102083965A (zh) 2011-06-01
US7939322B2 (en) 2011-05-10
BRPI0911599A2 (pt) 2020-08-18
KR101690773B1 (ko) 2016-12-28
USRE43876E1 (en) 2012-12-25
EP2669366A2 (en) 2013-12-04
RU2553538C2 (ru) 2015-06-20
CN102083965B (zh) 2014-10-15

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