CA2947663A1 - Microorganisme genetiquement modifie en vue d'une production amelioree de produits chimiques nobles a partir du saccharose - Google Patents
Microorganisme genetiquement modifie en vue d'une production amelioree de produits chimiques nobles a partir du saccharose Download PDFInfo
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- CA2947663A1 CA2947663A1 CA2947663A CA2947663A CA2947663A1 CA 2947663 A1 CA2947663 A1 CA 2947663A1 CA 2947663 A CA2947663 A CA 2947663A CA 2947663 A CA2947663 A CA 2947663A CA 2947663 A1 CA2947663 A1 CA 2947663A1
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- Prior art keywords
- gene
- nucleic acids
- deletion
- modified microorganism
- organic compound
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/11—Protein-serine/threonine kinases (2.7.11)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01018—Inositol 2-dehydrogenase (1.1.1.18)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y197/00—Other oxidoreductases (1.97)
- C12Y197/01—Other oxidoreductases (1.97) other oxidoreductases (1.97.1)
- C12Y197/01004—[Formate-C-acetyltransferase]-activating enzyme (1.97.1.4)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01054—Formate C-acetyltransferase (2.3.1.54), i.e. pyruvate formate-lyase or PFL
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
- C12Y207/0104—Pyruvate kinase (2.7.1.40)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/08—Transferases for other substituted phosphate groups (2.7.8)
- C12Y207/08031—Undecaprenyl-phosphate glucose phosphotransferase (2.7.8.31)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
La présente invention concerne un microorganisme modifié présentant, par rapport à son type sauvage, une activité réduite d'une enzyme encodée par le gène ptsA, une activité réduite d'une enzyme encodée par le gène ptsH ou une activité réduite d'une enzyme encodée par le gène ptsA et une activité réduite d'une enzyme encodée par le gène ptsH, le type sauvage dont est issu le microorganisme modifié appartenant à la famille des Pasteurellaceae. La présente invention concerne également un procédé de production d'acide succinique, ainsi que l'utilisation de microorganismes modifiés.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP14167488 | 2014-05-08 | ||
EP14167488.7 | 2014-05-08 | ||
PCT/EP2015/060102 WO2015169920A1 (fr) | 2014-05-08 | 2015-05-07 | Microorganisme génétiquement modifié en vue d'une production améliorée de produits chimiques nobles à partir du saccharose |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2947663A1 true CA2947663A1 (fr) | 2015-11-12 |
Family
ID=50679920
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2947663A Pending CA2947663A1 (fr) | 2014-05-08 | 2015-05-07 | Microorganisme genetiquement modifie en vue d'une production amelioree de produits chimiques nobles a partir du saccharose |
Country Status (9)
Country | Link |
---|---|
US (1) | US10287571B2 (fr) |
EP (1) | EP3140412A1 (fr) |
JP (1) | JP6608392B2 (fr) |
KR (1) | KR102308323B1 (fr) |
CN (1) | CN106536717A (fr) |
BR (1) | BR112016025791A2 (fr) |
CA (1) | CA2947663A1 (fr) |
MY (1) | MY193587A (fr) |
WO (2) | WO2015169920A1 (fr) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20160134760A (ko) * | 2014-03-19 | 2016-11-23 | 바스프 에스이 | 발효를 위한 탄수화물의 제한적 공급을 사용하는 글리세롤의 용도 |
US10301653B2 (en) | 2015-07-06 | 2019-05-28 | Wisconsin Alumni Research Foundation | Microorganisms that co-consume glucose with non-glucose carbohydrates and methods of use |
MX2018006535A (es) | 2015-12-02 | 2018-08-15 | Basf Se | Metodo para producir proteinas en hongos filamentosos con accion clr2 reducida. |
CN108291191A (zh) | 2015-12-02 | 2018-07-17 | 巴斯夫欧洲公司 | 在具有降低的clr1活性的丝状真菌中生产蛋白质的方法 |
AU2018246232B2 (en) | 2017-03-27 | 2023-12-07 | Tenfold Technologies, LLC | Methods and agricultural compositions for preventing or controlling plant diseases |
WO2018183383A1 (fr) * | 2017-03-27 | 2018-10-04 | Tenfold Technologies, LLC | Composition antipathogènes et procédés d'utilisation de celle-ci |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100679638B1 (ko) * | 2005-08-19 | 2007-02-06 | 한국과학기술원 | 포메이트 디하이드로게나제 d 또는 e를 코딩하는 유전자로 형질전환된 미생물 및 이를 이용한 숙신산의 제조방법 |
WO2010115067A2 (fr) * | 2009-04-02 | 2010-10-07 | University Of Florida Research Foundation, Inc. | Génie génétique et voie de production du succinate |
US20130305398A1 (en) * | 2012-02-16 | 2013-11-14 | Marie Coffin | Genes and uses for plant enhacement |
BRPI0815409B1 (pt) | 2007-08-17 | 2023-01-24 | Basf Se | Processos para a produção fermentativa de um composto, e para a produção de um composto, e, uso de uma cepa bacteriana |
ES2559385T3 (es) * | 2008-12-23 | 2016-02-11 | Basf Se | Células bacterianas que tienen una derivación de glioxilato para la fabricación de ácido succínico |
CA2751280C (fr) * | 2009-02-16 | 2019-03-12 | Basf Se | Nouveaux producteurs microbiens d'acide succinique et purification d'acide succinique |
WO2011063157A2 (fr) * | 2009-11-18 | 2011-05-26 | Myriant Technologies Llc | Production d'acide organique dans des micro-organismes par des voies du cycle des acides tricarboxyliques réductrices et oxydatives combinées |
KR101221557B1 (ko) * | 2010-08-30 | 2013-01-14 | 한국과학기술원 | 수크로오즈와 글리세롤을 동시에 이용하는 신규 숙신산 생성 변이 미생물 및 이를 이용한 숙신산 제조방법 |
US9187772B2 (en) | 2010-09-01 | 2015-11-17 | University Of Florida Research Foundation, Inc. | L-malate production by metabolically engineered escherichia coli |
WO2013003432A1 (fr) * | 2011-06-29 | 2013-01-03 | Genomatica, Inc. | Micro-organismes pour produire du succinate et procédés associés |
US9029136B2 (en) * | 2012-07-25 | 2015-05-12 | Glycosyn LLC | Alpha (1,2) fucosyltransferases suitable for use in the production of fucosylated oligosaccharides |
US9932611B2 (en) * | 2012-10-22 | 2018-04-03 | Genomatica, Inc. | Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing succinate related thereto |
-
2015
- 2015-05-07 EP EP15735633.8A patent/EP3140412A1/fr not_active Withdrawn
- 2015-05-07 CN CN201580037395.5A patent/CN106536717A/zh active Pending
- 2015-05-07 WO PCT/EP2015/060102 patent/WO2015169920A1/fr active Application Filing
- 2015-05-07 KR KR1020167034020A patent/KR102308323B1/ko active IP Right Grant
- 2015-05-07 WO PCT/EP2015/060100 patent/WO2015169919A1/fr active Application Filing
- 2015-05-07 MY MYPI2016001947A patent/MY193587A/en unknown
- 2015-05-07 BR BR112016025791A patent/BR112016025791A2/pt active Search and Examination
- 2015-05-07 US US15/308,874 patent/US10287571B2/en active Active
- 2015-05-07 JP JP2016567029A patent/JP6608392B2/ja active Active
- 2015-05-07 CA CA2947663A patent/CA2947663A1/fr active Pending
Also Published As
Publication number | Publication date |
---|---|
KR20170003630A (ko) | 2017-01-09 |
KR102308323B1 (ko) | 2021-10-06 |
BR112016025791A2 (pt) | 2018-01-16 |
JP2017514511A (ja) | 2017-06-08 |
CN106536717A (zh) | 2017-03-22 |
JP6608392B2 (ja) | 2019-11-20 |
US20170073665A1 (en) | 2017-03-16 |
US10287571B2 (en) | 2019-05-14 |
WO2015169920A1 (fr) | 2015-11-12 |
MY193587A (en) | 2022-10-19 |
WO2015169919A1 (fr) | 2015-11-12 |
EP3140412A1 (fr) | 2017-03-15 |
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