CA2915499A1 - Compositions and methods for sample processing - Google Patents

Compositions and methods for sample processing Download PDF

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Publication number
CA2915499A1
CA2915499A1 CA2915499A CA2915499A CA2915499A1 CA 2915499 A1 CA2915499 A1 CA 2915499A1 CA 2915499 A CA2915499 A CA 2915499A CA 2915499 A CA2915499 A CA 2915499A CA 2915499 A1 CA2915499 A1 CA 2915499A1
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CA
Canada
Prior art keywords
nucleic acid
bead
barcode
sequence
partitions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA2915499A
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English (en)
French (fr)
Inventor
Benjamin Hindson
Christopher HINDSON
Michael Schnall-Levin
Kevin Ness
Mirna Jarosz
Donald Masquelier
Serge Saxonov
Landon Merrill
Andrew Price
Paul Hardenbol
Yuan Li
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10X Genomics Inc
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10X Genomics Inc
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Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=52142836&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2915499(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US13/966,150 external-priority patent/US20140155295A1/en
Application filed by 10X Genomics Inc filed Critical 10X Genomics Inc
Publication of CA2915499A1 publication Critical patent/CA2915499A1/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149Particles, e.g. beads
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
CA2915499A 2013-06-27 2014-06-26 Compositions and methods for sample processing Pending CA2915499A1 (en)

Applications Claiming Priority (19)

Application Number Priority Date Filing Date Title
US201361840403P 2013-06-27 2013-06-27
US61/840,403 2013-06-27
US201361844804P 2013-07-10 2013-07-10
US61/844,804 2013-07-10
US13/966,150 2013-08-13
US13/966,150 US20140155295A1 (en) 2012-08-14 2013-08-13 Capsule array devices and methods of use
PCT/US2013/054797 WO2014028537A1 (en) 2012-08-14 2013-08-13 Microcapsule compositions and methods
USPCT/US2013/054797 2013-08-13
US201361896060P 2013-10-26 2013-10-26
US61/896,060 2013-10-26
US201361909974P 2013-11-27 2013-11-27
US61/909,974 2013-11-27
US201461937344P 2014-02-07 2014-02-07
US61/937,344 2014-02-07
US201461940318P 2014-02-14 2014-02-14
US61/940,318 2014-02-14
US201461991018P 2014-05-09 2014-05-09
US61/991,018 2014-05-09
PCT/US2014/044398 WO2014210353A2 (en) 2013-06-27 2014-06-26 Compositions and methods for sample processing

Publications (1)

Publication Number Publication Date
CA2915499A1 true CA2915499A1 (en) 2014-12-31

Family

ID=52142836

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2915499A Pending CA2915499A1 (en) 2013-06-27 2014-06-26 Compositions and methods for sample processing

Country Status (9)

Country Link
EP (3) EP3467160A1 (ko)
JP (1) JP6563912B2 (ko)
KR (4) KR102212234B1 (ko)
CN (3) CN110592182B (ko)
AU (3) AU2014302277A1 (ko)
BR (1) BR112015032512A8 (ko)
CA (1) CA2915499A1 (ko)
MX (1) MX361481B (ko)
WO (1) WO2014210353A2 (ko)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107427808A (zh) * 2015-01-12 2017-12-01 10X基因组学有限公司 用于制备核酸测序文库的方法和系统以及用其制备的文库
CN113272447A (zh) * 2018-11-08 2021-08-17 脱其泰有限责任公司 用于液滴中颗粒多重处理的系统和方法
CN113272449A (zh) * 2018-10-26 2021-08-17 Illumina公司 调整聚合物小珠以进行dna处理

Families Citing this family (165)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
ES2663234T3 (es) 2012-02-27 2018-04-11 Cellular Research, Inc Composiciones y kits para recuento molecular
EP2820174B1 (en) 2012-02-27 2019-12-25 The University of North Carolina at Chapel Hill Methods and uses for molecular tags
EP3495503A1 (en) 2012-03-05 2019-06-12 President and Fellows of Harvard College Systems and methods for epigenetic sequencing
US9567631B2 (en) 2012-12-14 2017-02-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN111748607B (zh) 2012-08-14 2024-04-30 10X基因组学有限公司 微胶囊组合物及方法
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN108753766A (zh) 2013-02-08 2018-11-06 10X基因组学有限公司 多核苷酸条形码生成
US9868979B2 (en) 2013-06-25 2018-01-16 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
CN110592182B (zh) 2013-06-27 2023-11-28 10X基因组学有限公司 用于样品处理的组合物和方法
EP3842542A1 (en) * 2013-08-28 2021-06-30 Becton, Dickinson and Company Massively parallel single cell analysis
JP2017504307A (ja) 2013-10-07 2017-02-09 セルラー リサーチ, インコーポレイテッド アレイ上のフィーチャーをデジタルカウントするための方法およびシステム
KR102596508B1 (ko) 2014-04-10 2023-10-30 10엑스 제노믹스, 인크. 시약을 캡슐화 및 구획화하기 위한 유체 디바이스, 시스템, 및 방법, 및 이의 응용
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
EP3161157B1 (en) * 2014-06-24 2024-03-27 Bio-Rad Laboratories, Inc. Digital pcr barcoding
JP2017523774A (ja) * 2014-06-26 2017-08-24 10エックス ジェノミクス, インコーポレイテッド サンプル分析のための方法及び組成物
CN106795553B (zh) 2014-06-26 2021-06-04 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
JP2017532042A (ja) 2014-10-29 2017-11-02 10エックス ゲノミクス,インコーポレイテッド 標的化核酸配列決定のための方法及び組成物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
CN107250379B (zh) 2015-02-19 2021-12-28 贝克顿迪金森公司 结合蛋白质组信息和基因组信息的高通量单细胞分析
US10697000B2 (en) 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
BR112017018054A2 (pt) * 2015-02-24 2018-07-24 10X Genomics Inc métodos para a cobertura de sequências de ácidos nucleicos direcionadas
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
US20180073073A1 (en) * 2015-03-18 2018-03-15 Cellular Research, Inc. Methods and compositions for labeling targets and haplotype phasing
WO2016160844A2 (en) * 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
AU2016248995B2 (en) * 2015-04-17 2022-04-28 President And Fellows Of Harvard College Barcoding systems and methods for gene sequencing and other applications
US10788452B2 (en) 2015-04-21 2020-09-29 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods for bacterial community relationship determination and other high throughput microbiology applications
EP3286326A1 (en) 2015-04-23 2018-02-28 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
EP3298168A4 (en) * 2015-05-18 2019-02-20 10X Genomics, Inc. STABILIZED REDUCING AGENTS AND METHODS OF USE
JP2018518950A (ja) * 2015-05-18 2018-07-19 10エックス ジェノミクス, インコーポレイテッド 生物医学的な反応及び分析で使用するための移動固相組成物
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
WO2016205728A1 (en) 2015-06-17 2016-12-22 Massachusetts Institute Of Technology Crispr mediated recording of cellular events
CN108026524A (zh) 2015-09-11 2018-05-11 赛卢拉研究公司 用于核酸文库标准化的方法和组合物
KR101651817B1 (ko) * 2015-10-28 2016-08-29 대한민국 Ngs 라이브러리 제작용 프라이머 세트 및 이를 이용한 ngs 라이브러리 제작방법 및 키트
WO2017075478A2 (en) 2015-10-28 2017-05-04 The Broad Institute Inc. Compositions and methods for evaluating and modulating immune responses by use of immune cell gene signatures
WO2017075294A1 (en) 2015-10-28 2017-05-04 The Board Institute Inc. Assays for massively combinatorial perturbation profiling and cellular circuit reconstruction
WO2017075451A1 (en) 2015-10-28 2017-05-04 The Broad Institute Inc. Compositions and methods for evaluating and modulating immune responses by detecting and targeting pou2af1
WO2017075465A1 (en) 2015-10-28 2017-05-04 The Broad Institute Inc. Compositions and methods for evaluating and modulating immune responses by detecting and targeting gata3
KR20180097536A (ko) * 2015-11-04 2018-08-31 아트레카, 인크. 단일 세포와 연관된 핵산의 분석을 위한 핵산 바코드의 조합 세트
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
CN108779460B (zh) * 2015-12-07 2023-08-01 睿恩达思技术公司 在分配中使用微粒的多路技术
CN109072288A (zh) 2016-05-02 2018-12-21 赛卢拉研究公司 精确的分子条形编码
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
EP3465502B1 (en) 2016-05-26 2024-04-10 Becton, Dickinson and Company Molecular label counting adjustment methods
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
JP7048105B2 (ja) * 2016-07-15 2022-04-05 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 核酸ライブラリを生成する方法
US11225689B2 (en) 2016-08-17 2022-01-18 The Broad Institute, Inc. Method for determination and identification of cell signatures and cell markers
WO2018049025A2 (en) 2016-09-07 2018-03-15 The Broad Institute Inc. Compositions and methods for evaluating and modulating immune responses
SG11201901733PA (en) 2016-09-26 2019-04-29 Cellular Res Inc Measurement of protein expression using reagents with barcoded oligonucleotide sequences
AU2017335897A1 (en) * 2016-09-28 2019-04-04 General Automation Lab Technologies, Inc. High resolution systems, kits, apparatus, and methods for bacterial community relationship determination and other high throughput microbiology applications
JP7232180B2 (ja) 2016-11-08 2023-03-02 ベクトン・ディキンソン・アンド・カンパニー 発現プロファイル分類の方法
EP3538672A1 (en) 2016-11-08 2019-09-18 Cellular Research, Inc. Methods for cell label classification
KR101909460B1 (ko) * 2016-11-21 2018-10-22 한국과학기술연구원 프라이머가 고정된 하이드로겔 미세입자 및 이를 이용한 핵산 증폭 방법
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) * 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018132610A1 (en) 2017-01-13 2018-07-19 Cellular Research, Inc. Hydrophilic coating of fluidic channels
CN110291207A (zh) 2017-01-27 2019-09-27 豪夫迈·罗氏有限公司 用于长程测序的条形码化dna
CN117512066A (zh) 2017-01-30 2024-02-06 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和系统
EP3577232A1 (en) 2017-02-01 2019-12-11 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
WO2018183908A1 (en) 2017-03-31 2018-10-04 Dana-Farber Cancer Institute, Inc. Compositions and methods for treating ovarian tumors
WO2018183921A1 (en) 2017-04-01 2018-10-04 The Broad Institute, Inc. Methods and compositions for detecting and modulating an immunotherapy resistance gene signature in cancer
WO2018191553A1 (en) 2017-04-12 2018-10-18 Massachusetts Eye And Ear Infirmary Tumor signature for metastasis, compositions of matter methods of use thereof
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
EP4215616A1 (en) 2017-05-18 2023-07-26 10X Genomics, Inc. Methods and systems for sorting droplets and beads
SG11201901822QA (en) * 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP3631012B1 (en) * 2017-05-26 2022-06-08 AbVitro LLC High-throughput polynucleotide library sequencing and transcriptome analysis
WO2018226546A1 (en) 2017-06-05 2018-12-13 10X Genomics, Inc. Gaskets for the distribution of pressures in a microfluidic system
AU2018281745B2 (en) 2017-06-05 2022-05-19 Becton, Dickinson And Company Sample indexing for single cells
WO2018232195A1 (en) 2017-06-14 2018-12-20 The Broad Institute, Inc. Compositions and methods targeting complement component 3 for inhibiting tumor growth
WO2019028047A1 (en) 2017-08-01 2019-02-07 Illumina, Inc SPATIAL INDEXING OF GENETIC MATERIAL AND PREPARATION OF PHARMACOTOQUE USING HYDROGEL BALLS AND FLOW CELLS
US20190064173A1 (en) 2017-08-22 2019-02-28 10X Genomics, Inc. Methods of producing droplets including a particle and an analyte
US11084037B2 (en) 2017-09-25 2021-08-10 Plexium, Inc. Oligonucleotide encoded chemical libraries
US20200255828A1 (en) 2017-10-04 2020-08-13 The Broad Institute, Inc. Methods and compositions for altering function and structure of chromatin loops and/or domains
US11680296B2 (en) 2017-10-16 2023-06-20 Massachusetts Institute Of Technology Mycobacterium tuberculosis host-pathogen interaction
WO2019079653A1 (en) 2017-10-19 2019-04-25 Ultima Genomics, Inc. METHODS OF TREATING APPARATUSED END SEQUENCES
WO2019084055A1 (en) 2017-10-23 2019-05-02 Massachusetts Institute Of Technology CLASSIFICATION OF GENETIC VARIATION FROM UNICELLULAR TRANSCRIPTOMS
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019113235A1 (en) * 2017-12-06 2019-06-13 10X Genomics, Inc. Methods and systems for processing nucleic acid molecules
US11332736B2 (en) 2017-12-07 2022-05-17 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
CN111492068A (zh) 2017-12-19 2020-08-04 贝克顿迪金森公司 与寡核苷酸相关联的颗粒
CN111712579A (zh) * 2017-12-22 2020-09-25 10X基因组学有限公司 用于处理来自一个或多个细胞的核酸分子的系统和方法
AU2019207900A1 (en) 2018-01-12 2020-07-09 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
EP3752832A1 (en) * 2018-02-12 2020-12-23 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11841371B2 (en) 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
CN112262218A (zh) 2018-04-06 2021-01-22 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
US11957695B2 (en) 2018-04-26 2024-04-16 The Broad Institute, Inc. Methods and compositions targeting glucocorticoid signaling for modulating immune responses
US11365409B2 (en) 2018-05-03 2022-06-21 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
ES2945191T3 (es) 2018-05-03 2023-06-29 Becton Dickinson Co Análisis de muestras multiómicas de alto rendimiento
US20210386829A1 (en) 2018-05-04 2021-12-16 The Broad Institute, Inc. Compositions and methods for modulating cgrp signaling to regulate innate lymphoid cell inflammatory responses
US11414701B2 (en) 2018-05-24 2022-08-16 The Broad Institute, Inc. Multimodal readouts for quantifying and sequencing nucleic acids in single cells
US20210371932A1 (en) 2018-06-01 2021-12-02 Massachusetts Institute Of Technology Methods and compositions for detecting and modulating microenvironment gene signatures from the csf of metastasis patients
JP2021526798A (ja) 2018-06-06 2021-10-11 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアThe Regents Of The University Of California 核酸ライブラリを生成する方法ならびにそれを実施するための組成物およびキット
WO2019241273A1 (en) 2018-06-11 2019-12-19 The Broad Institute, Inc. Lineage tracing using mitochondrial genome mutations and single cell genomics
CN112805389A (zh) 2018-10-01 2021-05-14 贝克顿迪金森公司 确定5’转录物序列
WO2020077135A1 (en) 2018-10-10 2020-04-16 Dana-Farber Cancer Institute, Inc. Modulating resistance to bcl-2 inhibitors
WO2020077236A1 (en) 2018-10-12 2020-04-16 The Broad Institute, Inc. Method for extracting nuclei or whole cells from formalin-fixed paraffin-embedded tissues
WO2020081730A2 (en) 2018-10-16 2020-04-23 Massachusetts Institute Of Technology Methods and compositions for modulating microenvironment
WO2020097315A1 (en) 2018-11-08 2020-05-14 Cellular Research, Inc. Whole transcriptome analysis of single cells using random priming
WO2020123301A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Generating spatial arrays with gradients
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
US20220062394A1 (en) 2018-12-17 2022-03-03 The Broad Institute, Inc. Methods for identifying neoantigens
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP3914728B1 (en) 2019-01-23 2023-04-05 Becton, Dickinson and Company Oligonucleotides associated with antibodies
US20220119871A1 (en) 2019-01-28 2022-04-21 The Broad Institute, Inc. In-situ spatial transcriptomics
WO2020167656A1 (en) 2019-02-11 2020-08-20 Ultima Genomics, Inc. Methods for nucleic acid analysis
US20230053540A1 (en) 2019-02-19 2023-02-23 Massachusetts Institute Of Technology Treatment of liver injury
EP3938537A1 (en) * 2019-03-11 2022-01-19 10X Genomics, Inc. Systems and methods for processing optically tagged beads
US20220154282A1 (en) 2019-03-12 2022-05-19 The Broad Institute, Inc. Detection means, compositions and methods for modulating synovial sarcoma cells
US20220143148A1 (en) 2019-03-14 2022-05-12 The Broad Institute, Inc. Compositions and methods for modulating cgrp signaling to regulate intestinal innate lymphoid cells
US20220142948A1 (en) 2019-03-18 2022-05-12 The Broad Institute, Inc. Compositions and methods for modulating metabolic regulators of t cell pathogenicity
US20220152148A1 (en) 2019-03-18 2022-05-19 The Broad Institute, Inc. Modulation of type 2 immunity by targeting clec-2 signaling
WO2020214642A1 (en) 2019-04-19 2020-10-22 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
EP4004231A1 (en) 2019-07-22 2022-06-01 Becton, Dickinson and Company Single cell chromatin immunoprecipitation sequencing assay
US20220282333A1 (en) 2019-08-13 2022-09-08 The General Hospital Corporation Methods for predicting outcomes of checkpoint inhibition and treatment thereof
US11793787B2 (en) 2019-10-07 2023-10-24 The Broad Institute, Inc. Methods and compositions for enhancing anti-tumor immunity by targeting steroidogenesis
GB2604481A (en) 2019-10-10 2022-09-07 1859 Inc Methods and systems for microfluidic screening
US11844800B2 (en) 2019-10-30 2023-12-19 Massachusetts Institute Of Technology Methods and compositions for predicting and preventing relapse of acute lymphoblastic leukemia
CN114729350A (zh) 2019-11-08 2022-07-08 贝克顿迪金森公司 使用随机引发获得用于免疫组库测序的全长v(d)j信息
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
US11857981B2 (en) 2019-12-23 2024-01-02 10X Genomics, Inc. Magnetic separator for an automated single cell sequencing system
US20230044602A1 (en) 2019-12-20 2023-02-09 EDWARD Via COLLEGE OF OSTEOPATHIC MEDICINE Cancer signatures, methods of generating cancer signatures, and uses thereof
WO2021146207A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and rna
IL272390A (en) 2020-01-30 2021-08-31 Yeda Res & Dev Cancer treatment methods
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
IL272586A (en) 2020-02-10 2021-08-31 Yeda Res & Dev A method for cell cluster analysis
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
ES2965354T3 (es) 2020-04-22 2024-04-12 10X Genomics Inc Métodos para análisis espacial que usan eliminación de ARN elegido como diana
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司(Us) 用于免疫组库谱分析的引物
EP4153776A1 (en) 2020-05-22 2023-03-29 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4153775A1 (en) 2020-05-22 2023-03-29 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
EP4158054A1 (en) 2020-06-02 2023-04-05 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
AU2021283174A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Nucleic acid library methods
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
IL278473A (en) 2020-11-03 2022-06-01 Yeda Res & Dev Methods for diagnosing and determining treatment in multiple myeloma
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4247967A1 (en) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profiling of highly expressed and lowly expressed proteins
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2022150662A1 (en) 2021-01-08 2022-07-14 10X Genomics, Inc. Methods for generating antigen-binding molecules from single cells
KR102320966B1 (ko) * 2021-02-15 2021-11-04 대한민국(식품의약품안전처장) 차세대염기서열분석법을 이용한 초위성체 마커 탐색 접근법
US20240150453A1 (en) 2021-03-09 2024-05-09 Massachusetts Institute Of Technology Methods of predicting response to anti-tnf blockade in inflammatory bowel disease
EP4196605A1 (en) 2021-09-01 2023-06-21 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2024076728A1 (en) 2022-10-06 2024-04-11 Dana-Farber Cancer Institute, Inc. Cyclic nucleotides and uses thereof

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5624711A (en) 1995-04-27 1997-04-29 Affymax Technologies, N.V. Derivatization of solid supports and methods for oligomer synthesis
WO2006030993A1 (en) * 2004-09-14 2006-03-23 Jin-Ho Choy Information code system using dna sequences
EP1931943A2 (en) * 2005-09-16 2008-06-18 The Regents of the University of California A colorimetric bio-barcode amplification assay for analyte detection
WO2007140015A2 (en) 2006-05-26 2007-12-06 Althea Technologies, Inc Biochemical analysis of partitioned cells
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
CN101241126A (zh) * 2008-03-14 2008-08-13 东华大学 一种生物条形码探针的制备方法
US20110218123A1 (en) 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
JP5457222B2 (ja) * 2009-02-25 2014-04-02 エフ.ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
EA023190B9 (ru) * 2009-04-02 2018-03-30 Флуидигм Корпорейшн Способ мультипраймерной амплификации для штрихового кодирования целевых нуклеиновых кислот
US8574835B2 (en) 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
US20120211084A1 (en) 2009-09-02 2012-08-23 President And Fellows Of Harvard College Multiple emulsions created using jetting and other techniques
US9255291B2 (en) * 2010-05-06 2016-02-09 Bioo Scientific Corporation Oligonucleotide ligation methods for improving data quality and throughput using massively parallel sequencing
EP2580378A4 (en) * 2010-06-08 2014-01-01 Nugen Technologies Inc MULTIPLEX SEQUENCING METHODS AND COMPOSITION
EP3561159B1 (en) * 2010-10-08 2023-09-20 President and Fellows of Harvard College High-throughput single cell barcoding
US9163281B2 (en) * 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
CN103443338B (zh) 2011-02-02 2017-09-22 华盛顿大学商业化中心 大规模平行邻接作图
EP3736281A1 (en) * 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
AU2012249759A1 (en) * 2011-04-25 2013-11-07 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
EP2817418B1 (en) * 2012-02-24 2017-10-11 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
AU2013293240A1 (en) 2012-07-24 2015-03-05 Adaptive Biotechnologies Corp. Single cell analysis using sequence tags
CN111748607B (zh) 2012-08-14 2024-04-30 10X基因组学有限公司 微胶囊组合物及方法
US20140378345A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20140378322A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US9567631B2 (en) 2012-12-14 2017-02-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2749653A1 (en) 2012-12-28 2014-07-02 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Molecular coding for analysis of composition of macromolecules and molecular complexes
CN110592182B (zh) 2013-06-27 2023-11-28 10X基因组学有限公司 用于样品处理的组合物和方法
US20150298091A1 (en) * 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107427808A (zh) * 2015-01-12 2017-12-01 10X基因组学有限公司 用于制备核酸测序文库的方法和系统以及用其制备的文库
CN107427808B (zh) * 2015-01-12 2020-10-23 10X基因组学有限公司 用于制备核酸测序文库的方法和系统以及用其制备的文库
CN113272449A (zh) * 2018-10-26 2021-08-17 Illumina公司 调整聚合物小珠以进行dna处理
CN113272449B (zh) * 2018-10-26 2024-03-12 Illumina公司 调整聚合物小珠以进行dna处理
CN113272447A (zh) * 2018-11-08 2021-08-17 脱其泰有限责任公司 用于液滴中颗粒多重处理的系统和方法

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