CA2907570A1 - Protocol for identifying and isolating antigen-specific b cells and producing antibodies to desired antigens - Google Patents
Protocol for identifying and isolating antigen-specific b cells and producing antibodies to desired antigensInfo
- Publication number
- CA2907570A1 CA2907570A1 CA2907570A CA2907570A CA2907570A1 CA 2907570 A1 CA2907570 A1 CA 2907570A1 CA 2907570 A CA2907570 A CA 2907570A CA 2907570 A CA2907570 A CA 2907570A CA 2907570 A1 CA2907570 A1 CA 2907570A1
- Authority
- CA
- Canada
- Prior art keywords
- cells
- antigen
- specific
- cell
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
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- G—PHYSICS
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5047—Cells of the immune system
- G01N33/5052—Cells of the immune system involving B-cells
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
- C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
- C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
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Landscapes
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| US201361791755P | 2013-03-15 | 2013-03-15 | |
| US61/791,755 | 2013-03-15 | ||
| PCT/US2014/030994 WO2014146074A2 (en) | 2013-03-15 | 2014-03-18 | Protocol for identifying and isolating antigen-specific b cells and producing antibodies to desired antigens |
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| CA2907570A1 true CA2907570A1 (en) | 2014-09-18 |
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| CA2907570A Abandoned CA2907570A1 (en) | 2013-03-15 | 2014-03-18 | Protocol for identifying and isolating antigen-specific b cells and producing antibodies to desired antigens |
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| US9255154B2 (en) | 2012-05-08 | 2016-02-09 | Alderbio Holdings, Llc | Anti-PCSK9 antibodies and use thereof |
| GB201316644D0 (en) | 2013-09-19 | 2013-11-06 | Kymab Ltd | Expression vector production & High-Throughput cell screening |
| CN111973740A (zh) | 2014-03-21 | 2020-11-24 | 泰华制药国际有限公司 | 针对降钙素基因相关肽的拮抗剂抗体及其使用方法 |
| US10556945B2 (en) | 2014-03-21 | 2020-02-11 | Teva Pharmaceuticals International Gmbh | Antagonist antibodies directed against calcitonin gene-related peptide and methods using same |
| EP3233916A4 (en) | 2014-12-19 | 2018-06-06 | Alder Biopharmaceuticals, Inc. | Humanized anti-acth antibodies and use thereof |
| US10934524B2 (en) | 2015-03-18 | 2021-03-02 | Epitomics, Inc. | High throughput monoclonal antibody generation by B cell panning and proliferation |
| US20190056398A1 (en) * | 2016-02-26 | 2019-02-21 | Sri International | Identification and isolation of antibodies from white blood cells |
| MX2018012470A (es) | 2016-04-15 | 2019-07-01 | Alder Biopharmaceuticals Inc | Anticuerpos anti pacap humanizados y usos de ellos. |
| CN109952314A (zh) | 2016-09-23 | 2019-06-28 | 泰瓦制药国际有限公司 | 治疗难治性偏头痛 |
| CA3045323A1 (en) | 2016-12-02 | 2018-06-07 | Juno Therapeutics, Inc. | Engineered b cells and related compositions and methods |
| WO2018133039A1 (zh) * | 2017-01-20 | 2018-07-26 | 深圳市新产业生物医学工程股份有限公司 | 制备抗体对的方法、试剂盒及试剂盒的应用和制备抗体对的系统 |
| US11125757B2 (en) | 2017-05-26 | 2021-09-21 | Emory University | Methods of culturing and characterizing antibody secreting cells |
| CN107058235B (zh) * | 2017-06-05 | 2020-08-11 | 深圳大学 | 一种b细胞筛选方法及其在单克隆抗体制备中的应用 |
| US20220243176A1 (en) * | 2019-05-31 | 2022-08-04 | The Penn State Research Foundation | Specific selection of immune cells using versatile display scaffolds |
| JP2023519782A (ja) * | 2020-01-17 | 2023-05-15 | ジャンプコード ゲノミクス，インク． | 標的化された配列決定の方法 |
| CN114441752B (zh) * | 2020-11-03 | 2024-11-26 | 中国科学院苏州纳米技术与纳米仿生研究所 | 一种筛选方法 |
| CN115166241B (zh) * | 2022-08-22 | 2023-03-24 | 广东忠信生物科技有限公司 | 一种同时筛选记忆b细胞和浆细胞的高效筛选技术及应用 |
| WO2025240335A1 (en) | 2024-05-13 | 2025-11-20 | Regeneron Pharmaceuticals, Inc. | Fgfr3 binding molecules and methods of use thereof |
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| WO1990000625A1 (en) * | 1988-07-14 | 1990-01-25 | Monoclonetics International, Inc. | Pre-screening for depletion and enrichment of specific b-cells |
| WO1992002551A1 (en) | 1990-08-02 | 1992-02-20 | B.R. Centre Limited | Methods for the production of proteins with a desired function |
| DK0488470T3 (da) * | 1990-11-26 | 1997-12-22 | Akzo Nobel Nv | Fremgangsmåde til fremstilling af antistoffer |
| US5213960A (en) * | 1992-03-09 | 1993-05-25 | Tanox Biosystems, Inc. | Methods for selecting low frequency antigen-specific single B lymphocytes |
| US5256542A (en) * | 1992-03-09 | 1993-10-26 | Tanox Biosystems, Inc. | Selecting low frequency antigen-specific single B lymphocytes with correction for background noise |
| US20050287607A1 (en) * | 2004-01-07 | 2005-12-29 | Diamond Betty A | Methods and compositions for identification of antigen-specific B cell populations |
| US20060051348A1 (en) | 2004-09-09 | 2006-03-09 | Jorn Gorlach | Method of producing a plurality of isolated antibodies to a plurality of cognate antigens |
| US7968308B2 (en) * | 2004-10-22 | 2011-06-28 | Danisco Us Inc. | Isolating human antibodies |
| EP1951337B1 (en) * | 2005-10-17 | 2018-08-01 | Gambro Lundia AB | Extracorporeal blood cleaning |
| EP2021463B1 (en) * | 2006-05-19 | 2016-11-23 | Alder Biopharmaceuticals, Inc. | Culture method for obtaining a clonal population of antigen-specific b cells |
| US20090191542A1 (en) * | 2006-08-23 | 2009-07-30 | Xcellerex, Inc. | Multi-variant cell indication technique |
| RU2459868C2 (ru) * | 2007-03-01 | 2012-08-27 | Симфоген А/С | Способ клонирования когнатных антител |
| CA3023889A1 (en) * | 2007-12-27 | 2009-07-09 | Abbott Laboratories | Anti-t. cruzi antibodies and methods of use |
| PL2400298T3 (pl) * | 2010-05-28 | 2014-01-31 | Hoffmann La Roche | Sposób hodowania pojedynczych komórek b oraz wytwarzania swoistych przeciwciał |
| EP2681237A1 (en) * | 2011-03-01 | 2014-01-08 | Novo Nordisk A/S | Antagonistic dr3 ligands |
| SG10201608341XA (en) * | 2011-11-23 | 2016-11-29 | Hoffmann La Roche | Cd40l expressing mammalian cells and their use |
| WO2013130462A1 (en) * | 2012-02-29 | 2013-09-06 | Medimmune, Llc | Antibody production methods |
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| IL241433B (en) | 2019-12-31 |
| KR20150140679A (ko) | 2015-12-16 |
| WO2014146074A3 (en) | 2014-11-06 |
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| AU2014232225B2 (en) | 2020-03-19 |
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| US20140287952A1 (en) | 2014-09-25 |
| AU2014232225A1 (en) | 2015-09-03 |
| JP6466397B2 (ja) | 2019-02-06 |
| WO2014146074A2 (en) | 2014-09-18 |
| EP2972330A2 (en) | 2016-01-20 |
| JP2016512044A (ja) | 2016-04-25 |
| US20160033504A1 (en) | 2016-02-04 |
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