CA2906365A1 - Essais a base d'arnase-h utilisant de monomeres d'arn modifies - Google Patents

Essais a base d'arnase-h utilisant de monomeres d'arn modifies

Info

Publication number
CA2906365A1
CA2906365A1 CA2906365A CA2906365A CA2906365A1 CA 2906365 A1 CA2906365 A1 CA 2906365A1 CA 2906365 A CA2906365 A CA 2906365A CA 2906365 A CA2906365 A CA 2906365A CA 2906365 A1 CA2906365 A1 CA 2906365A1
Authority
CA
Canada
Prior art keywords
rnase
enzyme
cleavage
primer
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2906365A
Other languages
English (en)
Inventor
Joseph Alan Walder
Mark Aaron Behlke
Scott D. Rose
Joseph R. Dobosy
Susan M. Rupp
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Integrated DNA Technologies Inc
Original Assignee
Integrated DNA Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/839,334 external-priority patent/US20140255925A9/en
Priority claimed from US13/959,637 external-priority patent/US9644198B2/en
Application filed by Integrated DNA Technologies Inc filed Critical Integrated DNA Technologies Inc
Publication of CA2906365A1 publication Critical patent/CA2906365A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés et des compositions pour améliorer la spécificité au cours de l'amplification d'une séquence d'ADN cible. Les procédés et compositions reposent sur l'utilisation d'une enzyme ARNase H, d'une polymérase et des amorces oligonucléotidiques sensibles à l'enzyme ARNase H, bloquées du point de vue du clivage, dans les réactions d'amplification, les mélanges de réaction comprenant soit une concentration finale optimisée d'un sel métallique divalent comprenant 2,0 mM ou moins de cation Mg++ libre et/ou une concentration finale optimisée d'un détergent non ionique comprenant au moins environ 0,001 % d'éther hexadécylique de polyéthylène glycol.
CA2906365A 2013-03-15 2013-12-02 Essais a base d'arnase-h utilisant de monomeres d'arn modifies Abandoned CA2906365A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US13/839,334 US20140255925A9 (en) 2008-04-30 2013-03-15 Modified rnase h enzymes and their uses
US13/839,334 2013-03-15
US13/959,637 2013-08-05
US13/959,637 US9644198B2 (en) 2008-04-30 2013-08-05 RNase H-based assays utilizing modified RNA monomers
PCT/US2013/072690 WO2014143228A1 (fr) 2013-03-15 2013-12-02 Essais à base d'arnase-h utilisant de monomères d'arn modifiés

Publications (1)

Publication Number Publication Date
CA2906365A1 true CA2906365A1 (fr) 2014-09-18

Family

ID=51537441

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2906365A Abandoned CA2906365A1 (fr) 2013-03-15 2013-12-02 Essais a base d'arnase-h utilisant de monomeres d'arn modifies

Country Status (6)

Country Link
EP (1) EP2971072A1 (fr)
JP (1) JP2016510601A (fr)
AU (1) AU2013381709A1 (fr)
CA (1) CA2906365A1 (fr)
SG (1) SG11201507512QA (fr)
WO (1) WO2014143228A1 (fr)

Families Citing this family (18)

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Publication number Priority date Publication date Assignee Title
EP3680348B1 (fr) * 2014-02-07 2023-04-12 University of Iowa Research Foundation Sondes à base d'oligonucléotides et procédés de détection de microbes
EP3034619B1 (fr) * 2014-12-16 2018-10-17 microBIOMix GmbH Oligonucléotide modifié et procédé d'éclusage de primaires ou de molécules de commande ou de sondes dans des systèmes PCR fermés
WO2017091811A2 (fr) 2015-11-25 2017-06-01 Integrated Dna Technologies, Inc. Méthodes de détection de variants
KR101901749B1 (ko) * 2016-02-15 2018-09-28 주식회사 누리바이오 실시간 핵산 또는 단백질 검출용 단일핵산 및 이를 이용한 검출 방법
US20190226033A1 (en) * 2016-09-27 2019-07-25 Nuribio Co., Ltd. Promer for Real-Time Detection of Nucleic Acid or Protein and Method of detecting Nucleic Acid or Protein Using the Same
CN112513265A (zh) * 2018-05-22 2021-03-16 博诚研究中心 用于人类癌症检测的修饰核酸的靶向富集和测序
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
US20210388415A1 (en) * 2018-10-24 2021-12-16 University Of Washington Methods and kits for depletion and enrichment of nucleic acid sequences
JP2023535407A (ja) * 2020-07-23 2023-08-17 インテグレーティッド ディーエヌエイ テクノロジーズ インコーポレーティッド 「ＣＴＬ－ｓｅｑ」（ＣＲＩＳＰＲ Ｔａｇ Ｌｉｎｅａｒ－ｓｅｑ）と称するヌクレアーゼオン／オフターゲット編集位置を指定するための方法
US20230167487A2 (en) 2021-07-12 2023-06-01 Labsimply, Inc. Crispr cascade assay
US11820983B2 (en) 2021-12-13 2023-11-21 Labsimply, Inc. Tuning cascade assay kinetics via molecular design
US20230279375A1 (en) 2021-12-13 2023-09-07 Labsimply, Inc. Signal boost cascade assay
GB202119080D0 (en) * 2021-12-29 2022-02-09 3Cr Bioscience Ltd Method
US12091689B2 (en) 2022-09-30 2024-09-17 Vedabio, Inc. Delivery of therapeutics in vivo via a CRISPR-based cascade system
WO2024076473A1 (fr) 2022-10-02 2024-04-11 Vedabio, Inc. Dosages de criblage de dimérisation
WO2024081156A1 (fr) 2022-10-14 2024-04-18 Vedabio, Inc. Détection de molécules cibles d'acide nucléique et d'acide non nucléique
US12091690B2 (en) 2023-01-07 2024-09-17 Vedabio, Inc. Engineered nucleic acid-guided nucleases
US12060602B2 (en) 2023-01-10 2024-08-13 Vedabio, Inc. Sample splitting for multiplexed detection of nucleic acids without amplification

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04349885A (ja) * 1991-05-29 1992-12-04 Teijin Ltd Ｃ型肝炎ウイルスのエンビロープ領域核酸断片およびその検出方法
EP0705905B1 (fr) * 1994-07-16 2001-10-10 Roche Diagnostics GmbH Procédé pour la détection sensible d'acides nucléiques
FR2724934B1 (fr) * 1994-09-26 1997-01-24 Bio Merieux Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique
US6274353B1 (en) * 1999-09-22 2001-08-14 Genecopoeia, Inc. Method and compositions for improved polynucleotide synthesis
CN100351393C (zh) * 2001-02-15 2007-11-28 宝生物工程株式会社 核苷酸多态性的检测方法
JP4257091B2 (ja) * 2001-10-09 2009-04-22 シスメックス株式会社 遺伝子増幅産物の検出方法及び遺伝子増幅産物の検出試薬キット
US20040175737A1 (en) * 2002-12-23 2004-09-09 Wyeth Assay for RNase H activity
WO2006081426A2 (fr) * 2005-01-28 2006-08-03 Applera Corporation Compositions et methodes pour completer une reaction de sequencage a un emplacement specifique d'un modele d'adn cible
ATE551349T1 (de) * 2005-11-30 2012-04-15 Epict Technologies Corp Methode unter verwendung von reversibel blockierten markierungsoligonukleotiden
JP2009284896A (ja) * 2007-07-26 2009-12-10 Fujifilm Corp 核酸増幅方法
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
JP5539325B2 (ja) * 2008-04-30 2014-07-02 インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ
CA2777446A1 (fr) * 2009-10-13 2011-04-21 Syntezza Molecular Detection Israel Ltd. Procedes et compositions pour amplifier des sequences cibles a partir d'echantillons d'acides nucleiques
EP2814975A4 (fr) * 2012-02-14 2015-12-30 Great Basin Scient Procédés d'amplification isotherme utilisant des amorces bloquées
WO2013142364A1 (fr) * 2012-03-19 2013-09-26 Integrated Dna Technologies, Inc. Enzymes rnase h modifiées et leurs utilisations

Also Published As

Publication number Publication date
AU2013381709A1 (en) 2015-10-01
SG11201507512QA (en) 2015-10-29
EP2971072A1 (fr) 2016-01-20
WO2014143228A1 (fr) 2014-09-18
JP2016510601A (ja) 2016-04-11

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Legal Events

Date Code Title Description
FZDE Discontinued

Effective date: 20191203

FZDE Discontinued

Effective date: 20191203