CA2895904C - Probability-directed isolation of nucleotide sequences (pins) - Google Patents

Probability-directed isolation of nucleotide sequences (pins) Download PDF

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Publication number
CA2895904C
CA2895904C CA2895904A CA2895904A CA2895904C CA 2895904 C CA2895904 C CA 2895904C CA 2895904 A CA2895904 A CA 2895904A CA 2895904 A CA2895904 A CA 2895904A CA 2895904 C CA2895904 C CA 2895904C
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Canada
Prior art keywords
target dna
dna molecule
sample
pcr
pins
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CA2895904A
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English (en)
French (fr)
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CA2895904A1 (en
Inventor
Thomas Kvist
Marie Just Mikkelsen
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Samplix ApS
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Samplix ApS
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2895904A 2012-12-21 2013-12-20 Probability-directed isolation of nucleotide sequences (pins) Active CA2895904C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA201270822 2012-12-21
DKPA201270822 2012-12-21
PCT/EP2013/077844 WO2014096421A1 (en) 2012-12-21 2013-12-20 Probability-directed isolation of nucleotide sequences (pins)

Publications (2)

Publication Number Publication Date
CA2895904A1 CA2895904A1 (en) 2014-06-26
CA2895904C true CA2895904C (en) 2021-06-08

Family

ID=49841687

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2895904A Active CA2895904C (en) 2012-12-21 2013-12-20 Probability-directed isolation of nucleotide sequences (pins)

Country Status (9)

Country Link
US (2) US10214759B2 (https=)
EP (1) EP2935617B1 (https=)
JP (1) JP6522511B2 (https=)
CN (1) CN105189781B (https=)
AU (1) AU2013366478A1 (https=)
CA (1) CA2895904C (https=)
DK (1) DK2935617T3 (https=)
ES (1) ES2630377T3 (https=)
WO (1) WO2014096421A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2013366478A1 (en) * 2012-12-21 2015-07-09 Samplix S.A.R.L. Probability-directed isolation of nucleotide sequences (pins)
US10738354B2 (en) 2015-06-26 2020-08-11 Samplix Aps Targeted enrichment of long nucleotide sequences using microfluidic partitioning
CN105420375B (zh) * 2015-12-24 2020-01-21 北京大学 一种环境微生物基因组草图的构建方法
CN109402223A (zh) * 2018-11-12 2019-03-01 河南弘腾农业有限公司 一种基于动物粪便样品构建测序文库的方法及其应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030228586A1 (en) 1999-06-30 2003-12-11 Rafick-Pierre Sekaly Method for measuring de novo T-cell production in humans
AU2003299694A1 (en) * 2002-12-20 2004-07-22 Qiagen Gmbh Nucleic acid amplification
KR100759390B1 (ko) 2007-02-05 2007-09-19 한국생명공학연구원 미배양 미생물의 단일세포 유래 게놈을 디지털 다중 분리증폭법을 이용하여 증폭하여 게놈 마이크로어레이를제작하는 방법
AU2013366478A1 (en) * 2012-12-21 2015-07-09 Samplix S.A.R.L. Probability-directed isolation of nucleotide sequences (pins)

Also Published As

Publication number Publication date
EP2935617B1 (en) 2017-04-12
US20160053291A1 (en) 2016-02-25
DK2935617T3 (en) 2017-07-17
CN105189781A (zh) 2015-12-23
US10214759B2 (en) 2019-02-26
JP2016500276A (ja) 2016-01-12
CA2895904A1 (en) 2014-06-26
JP6522511B2 (ja) 2019-05-29
US11078507B2 (en) 2021-08-03
AU2013366478A1 (en) 2015-07-09
WO2014096421A1 (en) 2014-06-26
CN105189781B (zh) 2017-06-06
EP2935617A1 (en) 2015-10-28
US20190233865A1 (en) 2019-08-01
ES2630377T3 (es) 2017-08-21

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