CA2894381C - Multiplex nucleic acid detection methods - Google Patents
Multiplex nucleic acid detection methods Download PDFInfo
- Publication number
- CA2894381C CA2894381C CA2894381A CA2894381A CA2894381C CA 2894381 C CA2894381 C CA 2894381C CA 2894381 A CA2894381 A CA 2894381A CA 2894381 A CA2894381 A CA 2894381A CA 2894381 C CA2894381 C CA 2894381C
- Authority
- CA
- Canada
- Prior art keywords
- probe
- nucleic acids
- target nucleic
- different
- amplicons
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/16—Assays for determining copy number or wherein the copy number is of special importance
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261734838P | 2012-12-07 | 2012-12-07 | |
| US61/734,838 | 2012-12-07 | ||
| PCT/US2013/073745 WO2014089536A1 (en) | 2012-12-07 | 2013-12-06 | Multiplex nucleic acid detection methods |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2894381A1 CA2894381A1 (en) | 2014-06-12 |
| CA2894381C true CA2894381C (en) | 2021-01-12 |
Family
ID=50884061
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2894381A Active CA2894381C (en) | 2012-12-07 | 2013-12-06 | Multiplex nucleic acid detection methods |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US9624533B2 (https=) |
| EP (1) | EP2929044B1 (https=) |
| JP (1) | JP6484177B2 (https=) |
| CN (1) | CN104995309B (https=) |
| BR (1) | BR112015013234B1 (https=) |
| CA (1) | CA2894381C (https=) |
| CL (1) | CL2015001568A1 (https=) |
| HK (1) | HK1216653A1 (https=) |
| IL (1) | IL239258B (https=) |
| WO (1) | WO2014089536A1 (https=) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL285521B2 (en) | 2013-08-19 | 2023-03-01 | Singular Bio Inc | Methods for single molecule detection |
| US20180023138A1 (en) * | 2014-11-01 | 2018-01-25 | Singular Bio, Inc. | Assays for Single Molecule Detection and Use Thereof |
| CN113528623A (zh) * | 2015-02-18 | 2021-10-22 | 卓异生物公司 | 用于单分子检测的测定及其应用 |
| CN107083427B (zh) * | 2016-04-01 | 2021-07-30 | 广州市基准医疗有限责任公司 | Dna连接酶介导的dna扩增技术 |
| WO2017181161A1 (en) * | 2016-04-15 | 2017-10-19 | Predicine, Inc. | Systems and methods for detecting genetic alterations |
| US11174503B2 (en) | 2016-09-21 | 2021-11-16 | Predicine, Inc. | Systems and methods for combined detection of genetic alterations |
| US11268137B2 (en) | 2016-12-09 | 2022-03-08 | Boreal Genomics, Inc. | Linked ligation |
| EP3565906B1 (en) * | 2017-01-05 | 2021-01-27 | Tervisetehnoloogiate Arenduskeskus AS | Quantifying dna sequences |
| US20180237853A1 (en) * | 2017-02-19 | 2018-08-23 | Yan Wang | Methods, Compositions and Kits for Detection of Mutant Variants of Target Genes |
| CN107177693B (zh) * | 2017-07-19 | 2020-11-06 | 中山大学 | 一种多倍pcr扩增方法 |
| WO2019046768A1 (en) * | 2017-08-31 | 2019-03-07 | William Marsh Rice University | SYMBOLIC SEQUENCING OF DNA AND RNA BY SEQUENCE CODING |
| CN110656159B (zh) * | 2018-06-28 | 2024-01-09 | 深圳华大生命科学研究院 | 一种拷贝数变异的检测方法 |
| JP7361774B2 (ja) * | 2018-07-27 | 2023-10-16 | ミリアド・ウィメンズ・ヘルス・インコーポレーテッド | シーケンスリードの独立したアラインメントおよびペアリングによって高度に相同なシーケンスにおける遺伝的変異を検出するための方法 |
| CN109402232A (zh) * | 2018-10-23 | 2019-03-01 | 中国农业大学 | 一种用于检测的组合物及检测方法 |
| AU2019375136B2 (en) * | 2018-11-05 | 2025-10-16 | Centre Henri Becquerel | Method for diagnosing a cancer and associated kit |
| CN115786455A (zh) * | 2022-07-12 | 2023-03-14 | 江阴检汇生物科技有限公司 | 一种基于mlpa-ngs方法用于司法鉴定的snp位点检测组合物及其应用 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5508179A (en) | 1994-03-18 | 1996-04-16 | Bio-Rad Laboratories, Inc. | Use of deoxyribose nicotinamide adenine dinucleotide to enhance the specificity of NAD+ -dependent ligation reactions |
| EP2369007B1 (en) * | 1996-05-29 | 2015-07-29 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
| US6544732B1 (en) | 1999-05-20 | 2003-04-08 | Illumina, Inc. | Encoding and decoding of array sensors utilizing nanocrystals |
| GB0400584D0 (en) | 2004-01-12 | 2004-02-11 | Solexa Ltd | Nucleic acid chacterisation |
| SE0401270D0 (sv) | 2004-05-18 | 2004-05-18 | Fredrik Dahl | Method for amplifying specific nucleic acids in parallel |
| EP1991698B1 (en) * | 2006-03-01 | 2013-12-18 | Keygene N.V. | High throughput sequence-based detection of snps using ligation assays |
| US8852864B2 (en) * | 2008-01-17 | 2014-10-07 | Sequenom Inc. | Methods and compositions for the analysis of nucleic acids |
| CA2757300C (en) * | 2009-04-01 | 2018-01-09 | Dxterity Diagnostics Incorporated | Chemical ligation dependent probe amplification (clpa) |
| CN102648295B (zh) * | 2009-12-07 | 2017-08-08 | 伊鲁米那股份有限公司 | 用于多重基因分型的多样品索引 |
| EP2526207A4 (en) * | 2010-01-22 | 2013-10-09 | Univ California | COMPOSITIONS AND METHODS FOR CHEMICAL SCREENING AT SIGNATURE BASE |
| WO2012018397A2 (en) | 2010-08-02 | 2012-02-09 | The Regents Of The University Of California | Molecular subtyping of oral squamous cell carcinoma to distinguish a subtype that is unlikely to metastasize |
| US9745616B2 (en) * | 2011-05-17 | 2017-08-29 | Dxterity Diagnostics Incorporated | Methods and compositions for detecting target nucleic acids |
-
2013
- 2013-12-06 WO PCT/US2013/073745 patent/WO2014089536A1/en not_active Ceased
- 2013-12-06 JP JP2015545900A patent/JP6484177B2/ja active Active
- 2013-12-06 CA CA2894381A patent/CA2894381C/en active Active
- 2013-12-06 US US14/099,821 patent/US9624533B2/en active Active
- 2013-12-06 CN CN201380072327.3A patent/CN104995309B/zh active Active
- 2013-12-06 EP EP13860702.3A patent/EP2929044B1/en active Active
- 2013-12-06 HK HK16104628.4A patent/HK1216653A1/zh unknown
- 2013-12-06 BR BR112015013234-0A patent/BR112015013234B1/pt active IP Right Grant
-
2015
- 2015-06-05 CL CL2015001568A patent/CL2015001568A1/es unknown
- 2015-06-07 IL IL239258A patent/IL239258B/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| HK1216653A1 (zh) | 2016-11-25 |
| EP2929044B1 (en) | 2019-02-06 |
| CA2894381A1 (en) | 2014-06-12 |
| CN104995309A (zh) | 2015-10-21 |
| EP2929044A4 (en) | 2016-07-13 |
| US9624533B2 (en) | 2017-04-18 |
| WO2014089536A1 (en) | 2014-06-12 |
| IL239258B (en) | 2019-12-31 |
| US20140235470A1 (en) | 2014-08-21 |
| JP6484177B2 (ja) | 2019-03-13 |
| JP2016500257A (ja) | 2016-01-12 |
| EP2929044A1 (en) | 2015-10-14 |
| BR112015013234B1 (pt) | 2021-12-21 |
| IL239258A0 (en) | 2015-07-30 |
| CN104995309B (zh) | 2020-12-08 |
| CL2015001568A1 (es) | 2016-08-26 |
| BR112015013234A2 (pt) | 2018-02-06 |
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| KR102079963B1 (ko) | 소거 프로브 기반 염색체 수적이상 검출방법 및 염색체 수적이상 검출용핵산의 조성물 | |
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| HK1188261B (en) | Compositions of toehold primer duplexes and methods of use |
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