CA2884266C - Production of organic acids by fermentation at low ph - Google Patents

Production of organic acids by fermentation at low ph Download PDF

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Publication number
CA2884266C
CA2884266C CA2884266A CA2884266A CA2884266C CA 2884266 C CA2884266 C CA 2884266C CA 2884266 A CA2884266 A CA 2884266A CA 2884266 A CA2884266 A CA 2884266A CA 2884266 C CA2884266 C CA 2884266C
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dehydrogenase
succinate
gene
yeast
malate
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French (fr)
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CA2884266A1 (en
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R. Rogers Yocum
Sudhanshu Dole
Janice G. Pero
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PTT Global Chemical PCL
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PTT Global Chemical PCL
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2884266A 2012-09-14 2013-09-13 Production of organic acids by fermentation at low ph Active CA2884266C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261701293P 2012-09-14 2012-09-14
US61/701,293 2012-09-14
PCT/US2013/059828 WO2014043591A1 (en) 2012-09-14 2013-09-13 Production of organic acids by fermentation at low ph

Publications (2)

Publication Number Publication Date
CA2884266A1 CA2884266A1 (en) 2014-03-20
CA2884266C true CA2884266C (en) 2022-05-24

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA2884266A Active CA2884266C (en) 2012-09-14 2013-09-13 Production of organic acids by fermentation at low ph

Country Status (9)

Country Link
US (1) US11072807B2 (cg-RX-API-DMAC10.html)
EP (1) EP2895593B1 (cg-RX-API-DMAC10.html)
JP (3) JP2015528312A (cg-RX-API-DMAC10.html)
KR (1) KR102253532B1 (cg-RX-API-DMAC10.html)
CN (1) CN104812889B (cg-RX-API-DMAC10.html)
BR (1) BR112015005416A2 (cg-RX-API-DMAC10.html)
CA (1) CA2884266C (cg-RX-API-DMAC10.html)
MY (1) MY182946A (cg-RX-API-DMAC10.html)
WO (1) WO2014043591A1 (cg-RX-API-DMAC10.html)

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WO2017151811A1 (en) * 2016-03-02 2017-09-08 Myriant Corporation Improved muconic acid production from genetically engineered microorganisms
EP3267345A1 (en) * 2016-07-06 2018-01-10 European Molecular Biology Laboratory Methods for adaptive laboratory evolution
KR20200023451A (ko) * 2017-06-30 2020-03-04 피티티 글로벌 케미칼 피씨엘 숙신산 생성이 증가된 유전자 변형 효모
CA3068459A1 (en) 2017-06-30 2019-01-03 Ptt Global Chemical Public Company Limited Microorganism with stabilized copy number of functional dna sequence and associated methods
WO2019159011A2 (en) 2018-02-16 2019-08-22 Ptt Global Chemical Public Company Limited Microorganisms and processes for lactic acid production
CN108676736B (zh) * 2018-04-20 2020-05-19 华中农业大学 一株可降解柠檬酸的毕赤酵母菌及其应用
CN112513261B (zh) * 2018-05-25 2024-07-19 丹尼斯科美国公司 富马酸还原酶过表达导致酵母中增加的发酵速率
WO2021034276A2 (en) * 2019-08-19 2021-02-25 Ptt Global Chemical Public Company Limited Yeast having improvement of lactic acid tolerance and use thereof
CN117467551A (zh) * 2022-07-29 2024-01-30 中国科学院天津工业生物技术研究所 一种高产l-苹果酸的耐酸酵母菌株及其构建方法和应用
CN119875861A (zh) * 2023-10-24 2025-04-25 中国石油化工股份有限公司 一株耐酸马克斯克鲁维酵母菌株及其在生产丁二酸中的应用
CN119162073A (zh) * 2024-11-01 2024-12-20 湖南省微生物研究院 一株柠檬酸杆菌基因工程菌株及其构建方法和应用
CN121801724A (zh) * 2026-03-09 2026-04-07 金发科技股份有限公司 一种工程化库德里亚兹毕赤酵母及其应用

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Also Published As

Publication number Publication date
WO2014043591A1 (en) 2014-03-20
CN104812889A (zh) 2015-07-29
BR112015005416A2 (pt) 2017-08-08
JP2015528312A (ja) 2015-09-28
KR102253532B1 (ko) 2021-05-18
US11072807B2 (en) 2021-07-27
CN104812889B (zh) 2018-08-28
US20150240270A1 (en) 2015-08-27
JP2018134108A (ja) 2018-08-30
MY182946A (en) 2021-02-05
KR20150055006A (ko) 2015-05-20
CA2884266A1 (en) 2014-03-20
JP6879976B2 (ja) 2021-06-02
EP2895593A4 (en) 2016-06-22
EP2895593B1 (en) 2019-12-11
JP2020127417A (ja) 2020-08-27
EP2895593A1 (en) 2015-07-22

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