CA2866587C - Analysis of measurements of a polymer - Google Patents

Analysis of measurements of a polymer Download PDF

Info

Publication number
CA2866587C
CA2866587C CA2866587A CA2866587A CA2866587C CA 2866587 C CA2866587 C CA 2866587C CA 2866587 A CA2866587 A CA 2866587A CA 2866587 A CA2866587 A CA 2866587A CA 2866587 C CA2866587 C CA 2866587C
Authority
CA
Canada
Prior art keywords
measurements
feature vector
polymer
derived
nanopore
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2866587A
Other languages
English (en)
French (fr)
Other versions
CA2866587A1 (en
Inventor
Stuart William Reid
James Anthony Clarke
James White
Gavin Harper
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford Nanopore Technologies PLC
Original Assignee
Oxford Nanopore Technologies PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford Nanopore Technologies PLC filed Critical Oxford Nanopore Technologies PLC
Publication of CA2866587A1 publication Critical patent/CA2866587A1/en
Application granted granted Critical
Publication of CA2866587C publication Critical patent/CA2866587C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48785—Electrical and electronic details of measuring devices for physical analysis of liquid biological material not specific to a particular test method, e.g. user interface or power supply
    • G01N33/48792—Data management, e.g. communication with processing unit
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6875—Nucleoproteins
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Nanotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Evolutionary Biology (AREA)
  • Theoretical Computer Science (AREA)
  • Cell Biology (AREA)
  • Databases & Information Systems (AREA)
  • Optics & Photonics (AREA)
  • Human Computer Interaction (AREA)
  • Electrochemistry (AREA)
CA2866587A 2012-02-16 2013-02-18 Analysis of measurements of a polymer Active CA2866587C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261599573P 2012-02-16 2012-02-16
US61/599,573 2012-02-16
PCT/GB2013/050381 WO2013121224A1 (en) 2012-02-16 2013-02-18 Analysis of measurements of a polymer

Publications (2)

Publication Number Publication Date
CA2866587A1 CA2866587A1 (en) 2013-08-22
CA2866587C true CA2866587C (en) 2022-07-26

Family

ID=47749886

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2866587A Active CA2866587C (en) 2012-02-16 2013-02-18 Analysis of measurements of a polymer

Country Status (10)

Country Link
US (3) US20150057948A1 (enExample)
EP (2) EP2814980B8 (enExample)
JP (1) JP6226888B2 (enExample)
KR (1) KR102106499B1 (enExample)
CN (1) CN104321441B (enExample)
AU (1) AU2013220179B2 (enExample)
BR (1) BR112014020211A2 (enExample)
CA (1) CA2866587C (enExample)
IN (1) IN2014DN06795A (enExample)
WO (1) WO2013121224A1 (enExample)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US8285719B1 (en) 2008-08-08 2012-10-09 The Research Foundation Of State University Of New York System and method for probabilistic relational clustering
JP6457811B2 (ja) 2011-09-23 2019-01-23 オックスフォード ナノポール テクノロジーズ リミテッド ポリマー単位を含むポリマーの解析
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
CN104321441B (zh) 2012-02-16 2016-10-19 牛津楠路珀尔科技有限公司 聚合物的测量的分析
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
EP4397972A3 (en) 2014-10-16 2024-10-09 Oxford Nanopore Technologies PLC Alignment mapping estimation
GB201418512D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Electrical device with detachable components
US10302972B2 (en) 2015-01-23 2019-05-28 Pacific Biosciences Of California, Inc. Waveguide transmission
EP3254103B1 (en) 2015-02-05 2023-08-16 President and Fellows of Harvard College Nanopore sensor including fluidic passage
GB201508669D0 (en) 2015-05-20 2015-07-01 Oxford Nanopore Tech Ltd Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown
WO2017024017A1 (en) 2015-08-06 2017-02-09 Pacific Biosciences Of California, Inc. Systems and methods for selectively addressing sparsely arranged electronic measurement devices
WO2017082875A1 (en) 2015-11-10 2017-05-18 Hewlett Packard Enterprise Development Lp Data allocation based on secure information retrieval
AU2017225374B2 (en) * 2016-03-02 2020-03-05 Oxford Nanopore Technologies Limited Mutant pore
US11514289B1 (en) 2016-03-09 2022-11-29 Freenome Holdings, Inc. Generating machine learning models using genetic data
US10823721B2 (en) 2016-07-05 2020-11-03 Quantapore, Inc. Optically based nanopore sequencing
GB201611770D0 (en) 2016-07-06 2016-08-17 Oxford Nanopore Tech Microfluidic device
KR102610098B1 (ko) * 2016-07-06 2023-12-04 가던트 헬쓰, 인크. 무세포 핵산의 프래그멘톰 프로파일링을 위한 방법
WO2018034745A1 (en) * 2016-08-18 2018-02-22 The Regents Of The University Of California Nanopore sequencing base calling
US11080301B2 (en) 2016-09-28 2021-08-03 Hewlett Packard Enterprise Development Lp Storage allocation based on secure data comparisons via multiple intermediaries
GB201619930D0 (en) 2016-11-24 2017-01-11 Oxford Nanopore Tech Apparatus and methods for controlling insertion of a membrane channel into a membrane
US20200251184A1 (en) * 2016-12-16 2020-08-06 Osaka University Classification analysis method, classification analysis device, and storage medium for classification analysis
GB201707138D0 (en) * 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Machine learning analysis of nanopore measurements
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
KR102620326B1 (ko) * 2017-09-15 2024-01-02 일루미나, 인코포레이티드 서열-검출 시스템
GB2568895B (en) 2017-11-29 2021-10-27 Oxford Nanopore Tech Ltd Microfluidic device
GB2569630B (en) 2017-12-21 2022-10-12 Sharp Life Science Eu Ltd Droplet Interfaces in Electro-wetting Devices
EP3731959B1 (en) 2017-12-29 2025-10-08 Clear Labs, Inc. Automated priming and library loading device
WO2019149626A1 (de) 2018-02-02 2019-08-08 Bayer Aktiengesellschaft Bekämpfung resistenter schadorganismen
WO2019204720A1 (en) * 2018-04-20 2019-10-24 The Regents Of The University Of California Nucleic acid sequencing methods and computer-readable media for practicing same
GB2574048B (en) 2018-05-24 2021-06-16 Oxford Nanopore Tech Ltd Nanopore sensor component with electrostatic discharge protection
US12269037B2 (en) 2018-05-24 2025-04-08 Oxford Nanopore Technologies Plc Droplet interfaces in electro-wetting devices
GB201814369D0 (en) * 2018-09-04 2018-10-17 Oxford Nanopore Tech Ltd Method for determining a polymersequence
JP7520833B2 (ja) 2018-11-28 2024-07-23 オックスフォード ナノポール テクノロジーズ ピーエルシー 感知システムおよび動作方法
GB201819378D0 (en) * 2018-11-28 2019-01-09 Oxford Nanopore Tech Ltd Analysis of nanopore signal using a machine-learning technique
US11789006B2 (en) 2019-03-12 2023-10-17 Oxford Nanopore Technologies Plc Nanopore sensing device, components and method of operation
EP3973077A4 (en) * 2019-05-22 2023-06-21 Icahn School of Medicine at Mount Sinai Method for de novo detection, identification and fine mapping of multiple forms of nucleic acid modifications
CN110628597B (zh) * 2019-10-19 2023-04-25 上海新微技术研发中心有限公司 拉曼光谱法生物分子测序方法
US11651281B2 (en) * 2020-05-18 2023-05-16 International Business Machines Corporation Feature catalog enhancement through automated feature correlation
WO2022013551A1 (en) 2020-07-17 2022-01-20 Oxford Nanopore Technologies Limited Nanopore sensing device
CN113030548A (zh) * 2021-03-09 2021-06-25 南京邮电大学 基于局域阈值的纳米孔检测电流信号特征信息提取方法及装置
GB202103605D0 (en) 2021-03-16 2021-04-28 Oxford Nanopore Tech Ltd Alignment of target and reference sequences of polymer units
WO2022243692A1 (en) 2021-05-19 2022-11-24 Oxford Nanopore Technologies Plc Methods for complement strand sequencing
WO2023029044A1 (zh) * 2021-09-06 2023-03-09 百图生科(北京)智能技术有限公司 用于单细胞测序的方法、装置、设备、介质和程序产品
CN113837036B (zh) * 2021-09-09 2024-08-02 成都齐碳科技有限公司 生物聚合物的表征方法、装置、设备及计算机存储介质
CN118835255B (zh) * 2022-04-14 2025-11-11 孔确(成都)科技有限公司 一种多个两亲性分子层的制备方法及装置
GB202215442D0 (en) 2022-10-19 2022-11-30 Oxford Nanopore Tech Plc Analysis of a polymer
WO2024099985A1 (en) 2022-11-10 2024-05-16 Bayer Aktiengesellschaft Targeted crop protection product application based on genetic profiles
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
JP7630538B2 (ja) * 2023-03-15 2025-02-17 Dic株式会社 樹脂物性値予測装置及び樹脂物性値の予測方法
GB202305524D0 (en) 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202307141D0 (en) 2023-05-12 2023-06-28 Oxford Nanopore Tech Plc Modified helicases

Family Cites Families (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6128587A (en) * 1997-01-14 2000-10-03 The Regents Of The University Of California Method and apparatus using Bayesian subfamily identification for sequence analysis
JPH11178575A (ja) * 1997-12-22 1999-07-06 Hitachi Ltd Ｄｎａ塩基配列解析装置、方法及び記録媒体
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
WO2000039333A1 (en) 1998-12-23 2000-07-06 Jones Elizabeth Louise Sequencing method using magnifying tags
AU5763000A (en) 1999-06-22 2001-01-09 President And Fellows Of Harvard College Molecular and atomic scale evaluation of biopolymers
AU2002239284A1 (en) 2000-11-27 2002-06-03 The Regents Of The University Of California Methods and devices for characterizing duplex nucleic acid molecules
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
JP2002325581A (ja) * 2001-04-27 2002-11-12 Adgene Co Ltd 核酸溶解曲線及び核酸解離曲線を用いた未知あるいは既知核酸変異検出法及び表示法
JP2003256435A (ja) 2002-03-06 2003-09-12 Fujitsu Ltd 配列データ統合処理方法、配列データ統合処理装置及び配列データ統合処理プログラム
US7220405B2 (en) 2003-09-08 2007-05-22 E. I. Du Pont De Nemours And Company Peptide-based conditioners and colorants for hair, skin, and nails
US20050136408A1 (en) 2003-12-19 2005-06-23 May Tom-Moy Methods and systems for characterizing a polymer
JP2005176730A (ja) 2003-12-19 2005-07-07 Hitachi Ltd ｃＤＮＡ配列をゲノム配列にマッピングする方法
US7279337B2 (en) 2004-03-10 2007-10-09 Agilent Technologies, Inc. Method and apparatus for sequencing polymers through tunneling conductance variation detection
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
KR100614827B1 (ko) 2004-05-06 2006-08-25 재단법인서울대학교산학협력재단 양방향 은닉 마코프 모델을 이용한 완숙한마이크로알엔에이 위치예측방법 및 이를 구현하기 위한컴퓨터 프로그램을 기록한 저장매체
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
US20060019259A1 (en) 2004-07-22 2006-01-26 Joyce Timothy H Characterization of biopolymers by resonance tunneling and fluorescence quenching
US20130071837A1 (en) 2004-10-06 2013-03-21 Stephen N. Winters-Hilt Method and System for Characterizing or Identifying Molecules and Molecular Mixtures
US20060086626A1 (en) 2004-10-22 2006-04-27 Joyce Timothy H Nanostructure resonant tunneling with a gate voltage source
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
US7960105B2 (en) 2005-11-29 2011-06-14 National Institutes Of Health Method of DNA analysis using micro/nanochannel
WO2007117832A2 (en) 2006-03-12 2007-10-18 Applera Corporation Methods of detecting target nucleic acids
WO2007137225A2 (en) 2006-05-19 2007-11-29 The University Of Chicago Method for indexing nucleic acid sequences for computer based searching
US7731826B2 (en) 2006-08-17 2010-06-08 Electronic Bio Sciences, Llc Controlled translocation of a polymer in an electrolytic sensing system
US8003319B2 (en) 2007-02-02 2011-08-23 International Business Machines Corporation Systems and methods for controlling position of charged polymer inside nanopore
EP2122344B8 (en) 2007-02-20 2019-08-21 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
US8500982B2 (en) 2007-04-04 2013-08-06 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
EP3540436B1 (en) 2007-09-12 2023-11-01 President And Fellows Of Harvard College High-resolution molecular sensor
GB2453377A (en) * 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
EP2307540B1 (en) 2008-07-07 2017-04-19 Oxford Nanopore Technologies Limited Enzyme-pore constructs
JP2011527191A (ja) * 2008-07-07 2011-10-27 オックスフォード ナノポア テクノロジーズ リミテッド 塩基検出細孔
ES2576114T3 (es) 2008-09-22 2016-07-05 University Of Washington Nanoporos MSP y procedimientos relacionados
CN102257162A (zh) 2008-10-29 2011-11-23 波士顿大学理事会 保持序列的dna转化
US8452546B1 (en) 2008-11-07 2013-05-28 Electronic Biosciences, Inc. Method for deducing a polymer sequence from a nominal base-by-base measurement
GB0820927D0 (en) * 2008-11-14 2008-12-24 Isis Innovation Method
BRPI1007215A2 (pt) 2009-01-30 2017-08-29 Oxford Nanopore Tech Ltd Método de acoplamento covalente de duas ou mais porções, primeira e segunda porções, primeiro porção acoplada a uma segunda porção, par de primeiro e segundo ligantes, e, uso de um par de ligantes.
WO2010086622A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
WO2010117470A2 (en) 2009-04-10 2010-10-14 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
JP5781502B2 (ja) 2009-04-20 2015-09-24 オックスフォード ナノポール テクノロジーズ リミテッド 脂質二重層センサアレイ
KR101732207B1 (ko) 2009-09-18 2017-05-02 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 고감도 분자 탐지 및 분석을 가능하게 하는 나노공극을 가지는 베어, 단일층 그래핀 멤브레인
CA2781581C (en) 2009-12-01 2018-03-06 Oxford Nanopore Technologies Limited Biochemical analysis instrument
US8748091B2 (en) 2009-12-18 2014-06-10 The Board Of Trustees Of The University Of Illinois Characterizing stretched polynucleotides in a synthetic nanopassage
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
ES2892158T3 (es) 2010-02-23 2022-02-02 Univ Washington Membranas artificiales de ácidos micólicos
US8828211B2 (en) 2010-06-08 2014-09-09 President And Fellows Of Harvard College Nanopore device with graphene supported artificial lipid membrane
CN103392008B (zh) 2010-09-07 2017-10-20 加利福尼亚大学董事会 通过持续性酶以一个核苷酸的精度控制dna在纳米孔中的移动
WO2012109483A2 (en) 2011-02-09 2012-08-16 Life Technologies Corporation A method of analysis of genetic markers
KR101939420B1 (ko) 2011-02-11 2019-01-16 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 세공
US20120316075A1 (en) 2011-03-30 2012-12-13 Noblegen Biosciences, Inc. Sequence preserved dna conversion for optical nanopore sequencing
EP4109094A1 (en) 2011-04-04 2022-12-28 President and Fellows of Harvard College Nanopore sensing by local electrical potential measurement
EP3848706B1 (en) 2011-05-27 2023-07-19 Oxford Nanopore Technologies PLC Coupling method
US9145623B2 (en) 2011-07-20 2015-09-29 Thermo Fisher Scientific Oy Transposon nucleic acids comprising a calibration sequence for DNA sequencing
BR112014001699A2 (pt) 2011-07-25 2017-06-13 Oxford Nanopore Tech Ltd método para sequenciar de um polinucleotídeo alvo de filamento duplo, kit, métodos para preparar um polinucleotídeo alvo de filamento duplo para sequenciamento e para sequenciar um polinucleotídeo alvo de filamento duplo, e, aparelho
JP6457811B2 (ja) 2011-09-23 2019-01-23 オックスフォード ナノポール テクノロジーズ リミテッド ポリマー単位を含むポリマーの解析
KR20140090633A (ko) 2011-10-21 2014-07-17 옥스포드 나노포어 테크놀로지즈 리미티드 포어 및 hel308 헬리카제를 사용하여 표적 폴리뉴클레오티드를 특성화하는 방법
EP2798083B1 (en) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
EP2798084B1 (en) 2011-12-29 2017-04-19 Oxford Nanopore Technologies Limited Enzyme method
WO2013109970A1 (en) 2012-01-20 2013-07-25 Genia Technologies, Inc. Nanopore based molecular detection and sequencing
CN104321441B (zh) 2012-02-16 2016-10-19 牛津楠路珀尔科技有限公司 聚合物的测量的分析
AU2013221356B2 (en) 2012-02-16 2018-10-18 The Regents Of The University Of California Nanopore sensor for enzyme-mediated protein translocation
CA2869546C (en) 2012-04-10 2020-07-21 Oxford Nanopore Technologies Limited Mutant lysenin pores
US10612083B2 (en) 2012-04-19 2020-04-07 University Of Washington Through Its Center For Commercialization Methods and compositions for generating reference maps for nanopore-based polymer analysis
GB2557509A (en) 2012-06-08 2018-06-20 Pacific Biosciences California Inc Modified base detection with nanopore sequencing
WO2014013262A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Enzyme construct
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
JP6614972B2 (ja) 2012-07-19 2019-12-04 オックスフォード ナノポール テクノロジーズ リミテッド 修飾ヘリカーゼ
WO2014064444A1 (en) 2012-10-26 2014-05-01 Oxford Nanopore Technologies Limited Droplet interfaces
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
US9689033B2 (en) 2013-11-26 2017-06-27 Illumina, Inc. Compositions and methods for polynucleotide sequencing
US20170096703A1 (en) 2014-03-21 2017-04-06 Oxford Nanopore Technologies Ltd. Analysis of a polymer from multi-dimensional measurements
GB201408652D0 (en) 2014-05-15 2014-07-02 Oxford Nanopore Tech Ltd Model adjustment during analysis of a polymer from nanopore measurements
US9925679B2 (en) 2014-05-19 2018-03-27 I+D+M Creative, Llc Devices and methods for assisting with slicing items
EP4397972A3 (en) 2014-10-16 2024-10-09 Oxford Nanopore Technologies PLC Alignment mapping estimation

Also Published As

Publication number Publication date
CA2866587A1 (en) 2013-08-22
EP2814980B8 (en) 2020-06-10
EP3736339A1 (en) 2020-11-11
AU2013220179A1 (en) 2014-09-04
EP3736339B1 (en) 2022-07-27
US20190154655A1 (en) 2019-05-23
CN104321441B (zh) 2016-10-19
US11959906B2 (en) 2024-04-16
JP6226888B2 (ja) 2017-11-08
EP2814980A1 (en) 2014-12-24
BR112014020211A2 (pt) 2017-07-04
US20240264143A1 (en) 2024-08-08
KR20140125874A (ko) 2014-10-29
KR102106499B1 (ko) 2020-05-04
CN104321441A (zh) 2015-01-28
AU2013220179B2 (en) 2018-11-08
JP2015509710A (ja) 2015-04-02
IN2014DN06795A (enExample) 2015-05-22
EP2814980B1 (en) 2020-04-15
WO2013121224A1 (en) 2013-08-22
US20150057948A1 (en) 2015-02-26

Similar Documents

Publication Publication Date Title
US20240264143A1 (en) Analysis of measurements of a polymer
US12486534B2 (en) Analysis of a polynucleotide via a nanopore system
US12545955B2 (en) Analysis of a polymer
Deamer et al. Three decades of nanopore sequencing
CN118120017A (zh) 纳米孔测量信号分析
WO2025238370A1 (en) Enhanced polynucleotide sequencing
Pasquarelli Genome Sequencing

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20180125

EEER Examination request

Effective date: 20180125

EEER Examination request

Effective date: 20180125

EEER Examination request

Effective date: 20180125

MPN Maintenance fee for patent paid

Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 12TH ANNIV.) - STANDARD

Year of fee payment: 12

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20250214

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT DETERMINED COMPLIANT

Effective date: 20250214