CA2865948A1 - System and method for time-resolved fluorescence imaging and pulse shaping - Google Patents
System and method for time-resolved fluorescence imaging and pulse shaping Download PDFInfo
- Publication number
- CA2865948A1 CA2865948A1 CA2865948A CA2865948A CA2865948A1 CA 2865948 A1 CA2865948 A1 CA 2865948A1 CA 2865948 A CA2865948 A CA 2865948A CA 2865948 A CA2865948 A CA 2865948A CA 2865948 A1 CA2865948 A1 CA 2865948A1
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- image
- fluorophore
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- 230000006870 function Effects 0.000 claims description 26
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- 238000005859 coupling reaction Methods 0.000 claims description 2
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- 238000000605 extraction Methods 0.000 abstract description 5
- 238000004422 calculation algorithm Methods 0.000 description 9
- 238000010586 diagram Methods 0.000 description 8
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6447—Fluorescence; Phosphorescence by visual observation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
- G01N2021/6413—Distinction short and delayed fluorescence or phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
- G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/062—LED's
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/062—LED's
- G01N2201/0621—Supply
Landscapes
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261605844P | 2012-03-02 | 2012-03-02 | |
| US61/605,844 | 2012-03-02 | ||
| PCT/US2013/028758 WO2013131062A1 (en) | 2012-03-02 | 2013-03-01 | System and method for time-resolved fluorescence imaging and pulse shaping |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2865948A1 true CA2865948A1 (en) | 2013-09-06 |
Family
ID=49083367
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2865948A Abandoned CA2865948A1 (en) | 2012-03-02 | 2013-03-01 | System and method for time-resolved fluorescence imaging and pulse shaping |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US10041883B2 (OSRAM) |
| EP (1) | EP2820398B1 (OSRAM) |
| JP (1) | JP6389763B2 (OSRAM) |
| AU (1) | AU2013225655A1 (OSRAM) |
| CA (1) | CA2865948A1 (OSRAM) |
| WO (1) | WO2013131062A1 (OSRAM) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2013225655A1 (en) | 2012-03-02 | 2014-09-18 | The Regents Of The University Of California | System and method for time-resolved fluorescence imaging and pulse shaping |
| WO2014168734A1 (en) | 2013-03-15 | 2014-10-16 | Cedars-Sinai Medical Center | Time-resolved laser-induced fluorescence spectroscopy systems and uses thereof |
| FR3013452B1 (fr) * | 2013-11-21 | 2016-02-05 | Centre Nat Rech Scient | Procede de detection d'une espece fluorescente reversiblement photoconvertible |
| JP6542044B2 (ja) | 2015-06-30 | 2019-07-10 | オリンパス株式会社 | 顕微鏡システム |
| CN108885173A (zh) | 2016-04-01 | 2018-11-23 | 黑光外科公司 | 用于时间分辨荧光光谱法的系统、装置和方法 |
| CA3032089A1 (en) * | 2016-08-03 | 2018-02-08 | Royal Melbourne Institute Of Technology | A method for distinguishing between more than one fluorescent species present in a sample |
| WO2018067355A1 (en) | 2016-10-04 | 2018-04-12 | The Regents Of The University Of California | Multi-frequency harmonic acoustography for target identification and border detection |
| KR101859172B1 (ko) * | 2016-12-22 | 2018-05-16 | (주) 인텍플러스 | 아날로그 평균지연 방법에서 수집한 신호로부터 가상의 형광분포 모델을 통해 최소자승오차를 계산하여 2개 이상의 형광수명 성분을 구하는 형광수명 측정장치 및 그 측정방법 |
| EP3704482A4 (en) * | 2017-11-01 | 2021-08-18 | The Regents of the University of California | PROCESS AND IMAGING SYSTEM FOR PEROPERATIVE ASSESSMENT OF SURGICAL MARGIN |
| KR102126694B1 (ko) | 2018-06-01 | 2020-06-25 | 주식회사 수젠텍 | 시간분해 형광신호 분석장치 |
| JP2020071152A (ja) * | 2018-10-31 | 2020-05-07 | ソニー株式会社 | 免疫染色方法、免疫染色システム、および免疫染色キット |
| CN113508327B (zh) * | 2019-02-01 | 2024-08-30 | 统雷有限公司 | 高动态范围成像 |
| US20210123921A1 (en) | 2019-10-17 | 2021-04-29 | C2Sense, Inc. | Diagnostic assays and related methods |
| JP2022552414A (ja) | 2019-10-17 | 2022-12-15 | シー２センス， インコーポレイテッド | 感知および／または認証のための発光イメージング |
| JP2023529260A (ja) | 2020-04-14 | 2023-07-10 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 光学画像誘導手術の選択的スペクトル照明のための方法及びシステム |
| US12209964B2 (en) * | 2020-10-15 | 2025-01-28 | Visera Technologies Company Limited | Bio-chip, bio-detection system and bio-detection method |
| US20240338792A1 (en) * | 2023-04-06 | 2024-10-10 | Thermo Electron Scientific Instruments Llc | Adaptive subtraction for c-sim microscopy |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4791310A (en) * | 1986-10-02 | 1988-12-13 | Syracuse University | Fluorescence microscopy |
| US4785495A (en) | 1987-08-17 | 1988-11-22 | Dellis Edward A | Moldable hand grips |
| JP2977746B2 (ja) * | 1995-08-23 | 1999-11-15 | 旭テクノグラス株式会社 | 蛍光ガラス線量計測定方法及びその測定装置 |
| US6272376B1 (en) * | 1999-01-22 | 2001-08-07 | Cedars-Sinai Medical Center | Time-resolved, laser-induced fluorescence for the characterization of organic material |
| US6690247B2 (en) * | 1999-02-05 | 2004-02-10 | Northrop Grumman Corporation | Nonlinear transmission line waveform generator having an input voltage matched to the C/V characteristic of the transmission line |
| US7890157B2 (en) | 2003-08-19 | 2011-02-15 | Cedars-Sinai Medical Center | Method for fluorescence lifetime imaging microscopy and spectroscopy |
| JP2005269051A (ja) * | 2004-03-17 | 2005-09-29 | Sysmex Corp | 発光ダイオードの駆動回路 |
| JP2008517280A (ja) * | 2004-10-18 | 2008-05-22 | マクオーリー ユニヴァーシティ | 蛍光検出 |
| US7193486B2 (en) * | 2005-01-19 | 2007-03-20 | Northrop Grumman Corporation | Tunable, maximum power output, frequency harmonic comb generator |
| JP2006226848A (ja) * | 2005-02-17 | 2006-08-31 | Olympus Corp | 蛍光撮像装置 |
| WO2006127967A2 (en) * | 2005-05-25 | 2006-11-30 | Massachusetts Institute Of Technology | Multifocal scanning microscopy systems and methods |
| US7648239B2 (en) | 2007-05-01 | 2010-01-19 | Richard Spaide | Autofluorescence photography using a fundus camera |
| JP4538033B2 (ja) * | 2007-09-10 | 2010-09-08 | 株式会社沖データ | 駆動回路、ｌｅｄヘッドおよび画像形成装置 |
| JP2010071982A (ja) * | 2008-08-20 | 2010-04-02 | Japan Atomic Energy Agency | レーザー誘起蛍光強度の減衰率比較によるアスベスト識別法 |
| GB0905690D0 (en) | 2009-04-01 | 2009-05-20 | Univ Cardiff | Fluorescence detection schemes |
| JP5437864B2 (ja) * | 2010-03-10 | 2014-03-12 | 富士フイルム株式会社 | ｐＨ測定装置の作動方法、検出装置の作動方法、及び生体物質分析装置の作動方法、並びに各装置 |
| JP2011185842A (ja) * | 2010-03-10 | 2011-09-22 | Fujifilm Corp | 光誘起自家蛍光の時間分解測定による生物試料の低酸素領域分析方法とその装置 |
| JP5626760B2 (ja) * | 2010-03-31 | 2014-11-19 | ソニー株式会社 | 蛍光像取得方法、蛍光像取得プログラム及び蛍光像取得装置 |
| GB201007055D0 (en) * | 2010-04-28 | 2010-06-09 | Vib Vzw | Method and apparatus for the imaging of a labelled sample |
| CA2814198A1 (en) | 2010-10-25 | 2012-05-10 | Steven Verdooner | Apparatus and method for detecting amyloid in a retina in a diagnosis, advancement, and prognosing of alzheimer's disease, traumatic brain injury, macular degeneration and a plurality of neurodegenerative dissorders, and ocular diseases |
| US10222335B2 (en) | 2010-10-27 | 2019-03-05 | The Regents Of The University Of California | Phasor method to fluorescence lifetime microscopy to discriminate metabolic state of cells in living tissue |
| AU2013225655A1 (en) | 2012-03-02 | 2014-09-18 | The Regents Of The University Of California | System and method for time-resolved fluorescence imaging and pulse shaping |
-
2013
- 2013-03-01 AU AU2013225655A patent/AU2013225655A1/en not_active Abandoned
- 2013-03-01 EP EP13755562.9A patent/EP2820398B1/en active Active
- 2013-03-01 WO PCT/US2013/028758 patent/WO2013131062A1/en not_active Ceased
- 2013-03-01 CA CA2865948A patent/CA2865948A1/en not_active Abandoned
- 2013-03-01 JP JP2014560114A patent/JP6389763B2/ja active Active
-
2014
- 2014-08-29 US US14/472,735 patent/US10041883B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2820398A4 (en) | 2016-01-20 |
| US10041883B2 (en) | 2018-08-07 |
| JP6389763B2 (ja) | 2018-09-12 |
| EP2820398A1 (en) | 2015-01-07 |
| US20150053871A1 (en) | 2015-02-26 |
| EP2820398B1 (en) | 2018-10-24 |
| JP2015508905A (ja) | 2015-03-23 |
| WO2013131062A1 (en) | 2013-09-06 |
| AU2013225655A1 (en) | 2014-09-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Dead |
Effective date: 20180301 |