CA2863635A1 - Selector based recognition and quantification system and method for multiple analytes in a single analysis - Google Patents
Selector based recognition and quantification system and method for multiple analytes in a single analysis Download PDFInfo
- Publication number
- CA2863635A1 CA2863635A1 CA2863635A CA2863635A CA2863635A1 CA 2863635 A1 CA2863635 A1 CA 2863635A1 CA 2863635 A CA2863635 A CA 2863635A CA 2863635 A CA2863635 A CA 2863635A CA 2863635 A1 CA2863635 A1 CA 2863635A1
- Authority
- CA
- Canada
- Prior art keywords
- analytes
- affinity
- dimension
- separation
- column
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims abstract description 84
- 238000004458 analytical method Methods 0.000 title description 99
- 238000011002 quantification Methods 0.000 title description 17
- 239000012491 analyte Substances 0.000 claims abstract description 90
- 238000000926 separation method Methods 0.000 claims abstract description 56
- 239000012488 sample solution Substances 0.000 claims abstract description 34
- 239000000126 substance Substances 0.000 claims abstract description 27
- 238000001179 sorption measurement Methods 0.000 claims abstract description 18
- 108090000623 proteins and genes Proteins 0.000 claims description 92
- 102000004169 proteins and genes Human genes 0.000 claims description 91
- 108090000631 Trypsin Proteins 0.000 claims description 45
- 102000004142 Trypsin Human genes 0.000 claims description 45
- 239000012588 trypsin Substances 0.000 claims description 44
- 108091034117 Oligonucleotide Proteins 0.000 claims description 27
- 239000012634 fragment Substances 0.000 claims description 26
- 230000008685 targeting Effects 0.000 claims description 21
- 108090001008 Avidin Proteins 0.000 claims description 7
- 108091023037 Aptamer Proteins 0.000 claims description 6
- 239000011324 bead Substances 0.000 claims description 6
- 230000006862 enzymatic digestion Effects 0.000 claims description 6
- 108090001090 Lectins Proteins 0.000 claims description 5
- 102000004856 Lectins Human genes 0.000 claims description 5
- 230000009144 enzymatic modification Effects 0.000 claims description 5
- 239000002523 lectin Substances 0.000 claims description 5
- 238000002823 phage display Methods 0.000 claims description 5
- 108010077805 Bacterial Proteins Proteins 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 102000000447 Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase Human genes 0.000 claims description 4
- 108010055817 Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase Proteins 0.000 claims description 4
- 230000029936 alkylation Effects 0.000 claims description 4
- 238000005804 alkylation reaction Methods 0.000 claims description 4
- 229940088598 enzyme Drugs 0.000 claims description 4
- 230000005661 hydrophobic surface Effects 0.000 claims description 4
- 230000009467 reduction Effects 0.000 claims description 4
- 102000053187 Glucuronidase Human genes 0.000 claims description 3
- 108010060309 Glucuronidase Proteins 0.000 claims description 3
- 108090000526 Papain Proteins 0.000 claims description 3
- 102000057297 Pepsin A Human genes 0.000 claims description 3
- 108090000284 Pepsin A Proteins 0.000 claims description 3
- 108010059712 Pronase Proteins 0.000 claims description 3
- 239000004365 Protease Substances 0.000 claims description 3
- ZADPBFCGQRWHPN-UHFFFAOYSA-N boronic acid Chemical compound OBO ZADPBFCGQRWHPN-UHFFFAOYSA-N 0.000 claims description 3
- 239000002738 chelating agent Substances 0.000 claims description 3
- 229910052751 metal Inorganic materials 0.000 claims description 3
- 239000002184 metal Substances 0.000 claims description 3
- 229940055729 papain Drugs 0.000 claims description 3
- 235000019834 papain Nutrition 0.000 claims description 3
- 229940111202 pepsin Drugs 0.000 claims description 3
- 108020003175 receptors Proteins 0.000 claims description 3
- 108091006027 G proteins Proteins 0.000 claims description 2
- 102000030782 GTP binding Human genes 0.000 claims description 2
- 108091000058 GTP-Binding Proteins 0.000 claims description 2
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims description 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims description 2
- 230000002163 immunogen Effects 0.000 claims description 2
- 239000012159 carrier gas Substances 0.000 claims 1
- 239000007789 gas Substances 0.000 claims 1
- 239000000427 antigen Substances 0.000 description 101
- 102000036639 antigens Human genes 0.000 description 101
- 108091007433 antigens Proteins 0.000 description 101
- 239000000523 sample Substances 0.000 description 55
- 108090000765 processed proteins & peptides Proteins 0.000 description 51
- 238000001514 detection method Methods 0.000 description 49
- 238000004949 mass spectrometry Methods 0.000 description 49
- 239000000243 solution Substances 0.000 description 46
- 102000004196 processed proteins & peptides Human genes 0.000 description 43
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 43
- 150000002500 ions Chemical class 0.000 description 38
- 239000002245 particle Substances 0.000 description 37
- 239000011159 matrix material Substances 0.000 description 28
- 239000012071 phase Substances 0.000 description 27
- 238000003556 assay Methods 0.000 description 25
- 239000011148 porous material Substances 0.000 description 25
- 241000894007 species Species 0.000 description 23
- 230000008569 process Effects 0.000 description 22
- 108020004414 DNA Proteins 0.000 description 20
- 239000000872 buffer Substances 0.000 description 20
- 230000017854 proteolysis Effects 0.000 description 19
- 238000013459 approach Methods 0.000 description 18
- 230000027455 binding Effects 0.000 description 18
- 230000035945 sensitivity Effects 0.000 description 17
- 239000002594 sorbent Substances 0.000 description 17
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 16
- 238000004587 chromatography analysis Methods 0.000 description 16
- 230000002378 acidificating effect Effects 0.000 description 15
- 239000000463 material Substances 0.000 description 15
- 230000009918 complex formation Effects 0.000 description 14
- 239000000203 mixture Substances 0.000 description 14
- 238000001542 size-exclusion chromatography Methods 0.000 description 14
- 239000003153 chemical reaction reagent Substances 0.000 description 13
- 230000007246 mechanism Effects 0.000 description 13
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 12
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 12
- 238000010828 elution Methods 0.000 description 12
- 238000009792 diffusion process Methods 0.000 description 11
- 230000007717 exclusion Effects 0.000 description 11
- 230000002209 hydrophobic effect Effects 0.000 description 11
- 239000003547 immunosorbent Substances 0.000 description 11
- 108010093096 Immobilized Enzymes Proteins 0.000 description 10
- 108091093037 Peptide nucleic acid Proteins 0.000 description 10
- 238000002965 ELISA Methods 0.000 description 9
- 230000008901 benefit Effects 0.000 description 9
- 230000000295 complement effect Effects 0.000 description 9
- 238000002330 electrospray ionisation mass spectrometry Methods 0.000 description 9
- 238000010324 immunological assay Methods 0.000 description 9
- 238000003314 affinity selection Methods 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 8
- 238000000132 electrospray ionisation Methods 0.000 description 8
- 230000006870 function Effects 0.000 description 8
- 238000000816 matrix-assisted laser desorption--ionisation Methods 0.000 description 8
- 238000002835 absorbance Methods 0.000 description 7
- 230000015572 biosynthetic process Effects 0.000 description 7
- 238000001360 collision-induced dissociation Methods 0.000 description 7
- 230000029087 digestion Effects 0.000 description 7
- 238000010494 dissociation reaction Methods 0.000 description 7
- 230000005593 dissociations Effects 0.000 description 7
- 230000003993 interaction Effects 0.000 description 7
- 238000004811 liquid chromatography Methods 0.000 description 7
- 238000005259 measurement Methods 0.000 description 7
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 6
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 6
- 229960002685 biotin Drugs 0.000 description 6
- 235000020958 biotin Nutrition 0.000 description 6
- 239000011616 biotin Substances 0.000 description 6
- 238000012875 competitive assay Methods 0.000 description 6
- 238000001212 derivatisation Methods 0.000 description 6
- -1 hapten Substances 0.000 description 6
- 230000001965 increasing effect Effects 0.000 description 6
- 238000012856 packing Methods 0.000 description 6
- 239000011347 resin Substances 0.000 description 6
- 229920005989 resin Polymers 0.000 description 6
- 108060003951 Immunoglobulin Proteins 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 5
- 238000007385 chemical modification Methods 0.000 description 5
- 239000011248 coating agent Substances 0.000 description 5
- 238000000576 coating method Methods 0.000 description 5
- 238000001077 electron transfer detection Methods 0.000 description 5
- 238000002101 electrospray ionisation tandem mass spectrometry Methods 0.000 description 5
- 238000005755 formation reaction Methods 0.000 description 5
- 230000001900 immune effect Effects 0.000 description 5
- 102000018358 immunoglobulin Human genes 0.000 description 5
- 230000002452 interceptive effect Effects 0.000 description 5
- 238000001499 laser induced fluorescence spectroscopy Methods 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 239000000377 silicon dioxide Substances 0.000 description 5
- 239000002699 waste material Substances 0.000 description 5
- 238000003491 array Methods 0.000 description 4
- 238000004925 denaturation Methods 0.000 description 4
- 230000036425 denaturation Effects 0.000 description 4
- 239000003480 eluent Substances 0.000 description 4
- 238000005194 fractionation Methods 0.000 description 4
- 229940072221 immunoglobulins Drugs 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 238000005040 ion trap Methods 0.000 description 4
- 230000000670 limiting effect Effects 0.000 description 4
- 230000014759 maintenance of location Effects 0.000 description 4
- 239000002244 precipitate Substances 0.000 description 4
- 238000004513 sizing Methods 0.000 description 4
- 239000007790 solid phase Substances 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 102000014914 Carrier Proteins Human genes 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 108090000288 Glycoproteins Proteins 0.000 description 3
- 102000003886 Glycoproteins Human genes 0.000 description 3
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 3
- 102000001708 Protein Isoforms Human genes 0.000 description 3
- 108010029485 Protein Isoforms Proteins 0.000 description 3
- 101710120037 Toxin CcdB Proteins 0.000 description 3
- 206010053614 Type III immune complex mediated reaction Diseases 0.000 description 3
- 239000003929 acidic solution Substances 0.000 description 3
- 239000003463 adsorbent Substances 0.000 description 3
- 239000002585 base Substances 0.000 description 3
- 108091008324 binding proteins Proteins 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000000835 electrochemical detection Methods 0.000 description 3
- 239000012149 elution buffer Substances 0.000 description 3
- 238000001825 field-flow fractionation Methods 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 3
- 238000009396 hybridization Methods 0.000 description 3
- 230000016178 immune complex formation Effects 0.000 description 3
- 210000000987 immune system Anatomy 0.000 description 3
- 238000011068 loading method Methods 0.000 description 3
- 238000005191 phase separation Methods 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 230000004481 post-translational protein modification Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 230000002797 proteolythic effect Effects 0.000 description 3
- 150000003384 small molecules Chemical class 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- HGINCPLSRVDWNT-UHFFFAOYSA-N Acrolein Chemical compound C=CC=O HGINCPLSRVDWNT-UHFFFAOYSA-N 0.000 description 2
- 108010032595 Antibody Binding Sites Proteins 0.000 description 2
- 108020004635 Complementary DNA Proteins 0.000 description 2
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 2
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 2
- 108090001030 Lipoproteins Proteins 0.000 description 2
- 102000004895 Lipoproteins Human genes 0.000 description 2
- 102000007474 Multiprotein Complexes Human genes 0.000 description 2
- 108010085220 Multiprotein Complexes Proteins 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 108010033276 Peptide Fragments Proteins 0.000 description 2
- 102000007079 Peptide Fragments Human genes 0.000 description 2
- 239000002262 Schiff base Substances 0.000 description 2
- 150000004753 Schiff bases Chemical class 0.000 description 2
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 150000001299 aldehydes Chemical class 0.000 description 2
- 125000003275 alpha amino acid group Chemical group 0.000 description 2
- 239000011230 binding agent Substances 0.000 description 2
- 238000010804 cDNA synthesis Methods 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 230000000536 complexating effect Effects 0.000 description 2
- 238000010668 complexation reaction Methods 0.000 description 2
- 238000004132 cross linking Methods 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 150000002009 diols Chemical class 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000011067 equilibration Methods 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 125000000524 functional group Chemical group 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000003100 immobilizing effect Effects 0.000 description 2
- 238000005342 ion exchange Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- YTJSFYQNRXLOIC-UHFFFAOYSA-N octadecylsilane Chemical compound CCCCCCCCCCCCCCCCCC[SiH3] YTJSFYQNRXLOIC-UHFFFAOYSA-N 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 108091033319 polynucleotide Proteins 0.000 description 2
- 102000040430 polynucleotide Human genes 0.000 description 2
- 239000002157 polynucleotide Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000000527 sonication Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- CHRJZRDFSQHIFI-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;styrene Chemical compound C=CC1=CC=CC=C1.C=CC1=CC=CC=C1C=C CHRJZRDFSQHIFI-UHFFFAOYSA-N 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241001015476 Botria Species 0.000 description 1
- 108020004394 Complementary RNA Proteins 0.000 description 1
- 241000283073 Equus caballus Species 0.000 description 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical group [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- 241000473945 Theria <moth genus> Species 0.000 description 1
- 108010034949 Thyroglobulin Proteins 0.000 description 1
- 102000009843 Thyroglobulin Human genes 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 125000003172 aldehyde group Chemical group 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- SFZBBUSDVJSDGR-XWFYHZIMSA-N beta-D-Galp-(1->4)-[alpha-L-Fucp-(1->3)]-beta-D-GlcpNAc-(1->3)-beta-D-Galp Chemical compound O[C@H]1[C@H](O)[C@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](O[C@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)[C@@H](CO)O[C@@H](O[C@H]2[C@H]([C@@H](CO)O[C@@H](O)[C@@H]2O)O)[C@@H]1NC(C)=O SFZBBUSDVJSDGR-XWFYHZIMSA-N 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 150000001793 charged compounds Chemical class 0.000 description 1
- 150000005829 chemical entities Chemical class 0.000 description 1
- 239000012504 chromatography matrix Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000009137 competitive binding Effects 0.000 description 1
- 239000003184 complementary RNA Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000022811 deglycosylation Effects 0.000 description 1
- 239000003398 denaturant Substances 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000001516 effect on protein Effects 0.000 description 1
- 238000000840 electrochemical analysis Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000003028 elevating effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000012921 fluorescence analysis Methods 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 238000010574 gas phase reaction Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- RBNPOMFGQQGHHO-UHFFFAOYSA-N glyceric acid Chemical compound OCC(O)C(O)=O RBNPOMFGQQGHHO-UHFFFAOYSA-N 0.000 description 1
- 238000003505 heat denaturation Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 description 1
- 238000001597 immobilized metal affinity chromatography Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000010954 inorganic particle Substances 0.000 description 1
- 238000005305 interferometry Methods 0.000 description 1
- 238000010813 internal standard method Methods 0.000 description 1
- 239000012500 ion exchange media Substances 0.000 description 1
- 238000000752 ionisation method Methods 0.000 description 1
- 230000000155 isotopic effect Effects 0.000 description 1
- 238000001948 isotopic labelling Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 1
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000003032 molecular docking Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- QPUKANZXOGADOB-UHFFFAOYSA-N n-dodecyl-n-methylnitrous amide Chemical compound CCCCCCCCCCCCN(C)N=O QPUKANZXOGADOB-UHFFFAOYSA-N 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000007030 peptide scission Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 150000003141 primary amines Chemical class 0.000 description 1
- 230000006916 protein interaction Effects 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000006722 reduction reaction Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000002098 selective ion monitoring Methods 0.000 description 1
- 239000012279 sodium borohydride Substances 0.000 description 1
- 229910000033 sodium borohydride Inorganic materials 0.000 description 1
- BEOOHQFXGBMRKU-UHFFFAOYSA-N sodium cyanoborohydride Chemical compound [Na+].[B-]C#N BEOOHQFXGBMRKU-UHFFFAOYSA-N 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 1
- 238000004885 tandem mass spectrometry Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229960002175 thyroglobulin Drugs 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- YUYCVXFAYWRXLS-UHFFFAOYSA-N trimethoxysilane Chemical compound CO[SiH](OC)OC YUYCVXFAYWRXLS-UHFFFAOYSA-N 0.000 description 1
- 230000004304 visual acuity Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261594193P | 2012-02-02 | 2012-02-02 | |
| US61/594,193 | 2012-02-02 | ||
| PCT/US2013/023727 WO2013116260A1 (en) | 2012-02-02 | 2013-01-30 | Selector based recognition and quantification system and method for multiple analytes in a single analysis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2863635A1 true CA2863635A1 (en) | 2013-08-08 |
Family
ID=48905766
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2863635A Abandoned CA2863635A1 (en) | 2012-02-02 | 2013-01-30 | Selector based recognition and quantification system and method for multiple analytes in a single analysis |
Country Status (12)
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015084748A1 (en) * | 2013-12-03 | 2015-06-11 | Perfinity Biosciences, Inc | A single reactor for simplified sample preparation workflows |
| WO2016118489A1 (en) * | 2015-01-19 | 2016-07-28 | Siscapa Assay Technologies, Inc. | Combined analysis of small molecules and proteins by mass spectrometry |
| CN111902720B (zh) * | 2018-03-21 | 2025-02-07 | 沃特世科技公司 | 基于非抗体高亲和力的样品制备、吸附剂、装置和方法 |
| CN112020652A (zh) * | 2018-04-30 | 2020-12-01 | 百特基公司 | 蛋白质的定性分析 |
| WO2019229058A1 (en) * | 2018-05-29 | 2019-12-05 | Capsenze Biosystems Ab | Method and system for providing antibody fragments suitable for online quality control |
| KR102198342B1 (ko) * | 2019-05-22 | 2021-01-04 | 연세대학교 산학협력단 | 항체 선별 방법 및 이를 이용한 항체 선별 시스템 |
| DE102020113317A1 (de) * | 2020-05-15 | 2021-11-18 | Endress+Hauser Conducta Gmbh+Co. Kg | Automatische Probennahmevorrichtung und Verfahren zum automatisierten Bereitstellen einer Probe für eine qualitative und/oder quantitative Bestimmung eines Analyten |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5061790A (en) * | 1989-07-10 | 1991-10-29 | Molecular Diagnostics, Inc. | Oxidative denaturation of protein analytes |
| US6303325B1 (en) * | 1998-05-29 | 2001-10-16 | Dade Behring Inc. | Method for detecting analytes |
| US7449170B2 (en) * | 2001-09-27 | 2008-11-11 | Purdue Research Foundation | Materials and methods for controlling isotope effects during fractionation of analytes |
| CA2722435A1 (en) * | 2008-04-23 | 2009-10-29 | Luminex Corporation | Method for creating a standard for multiple analytes found in a starting material of biological origin |
| WO2010132453A2 (en) * | 2009-05-11 | 2010-11-18 | Nexus Dx, Inc. | Methods and compositions for analyte detection |
| US8455202B2 (en) * | 2010-03-10 | 2013-06-04 | Perfinity Biosciences, Inc. | Affinity selector based recognition and quantification system and method for multiple analytes in a single analysis |
-
2013
- 2013-01-30 SG SG11201404526PA patent/SG11201404526PA/en unknown
- 2013-01-30 CN CN201380013470.5A patent/CN104160278A/zh active Pending
- 2013-01-30 KR KR20147024464A patent/KR20140137353A/ko not_active Withdrawn
- 2013-01-30 AU AU2013215305A patent/AU2013215305A1/en not_active Abandoned
- 2013-01-30 JP JP2014555635A patent/JP2015505619A/ja active Pending
- 2013-01-30 WO PCT/US2013/023727 patent/WO2013116260A1/en active Application Filing
- 2013-01-30 IN IN6899DEN2014 patent/IN2014DN06899A/en unknown
- 2013-01-30 CA CA2863635A patent/CA2863635A1/en not_active Abandoned
- 2013-01-30 EP EP13742988.2A patent/EP2820425A1/en not_active Withdrawn
- 2013-01-30 BR BR112014019134A patent/BR112014019134A8/pt not_active IP Right Cessation
- 2013-01-30 RU RU2014135576A patent/RU2014135576A/ru unknown
-
2014
- 2014-07-31 IL IL233897A patent/IL233897A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BR112014019134A8 (pt) | 2017-07-11 |
| CN104160278A (zh) | 2014-11-19 |
| JP2015505619A (ja) | 2015-02-23 |
| RU2014135576A (ru) | 2016-03-27 |
| SG11201404526PA (en) | 2014-11-27 |
| WO2013116260A1 (en) | 2013-08-08 |
| EP2820425A1 (en) | 2015-01-07 |
| KR20140137353A (ko) | 2014-12-02 |
| IL233897A0 (en) | 2014-09-30 |
| BR112014019134A2 (enrdf_load_stackoverflow) | 2017-06-20 |
| AU2013215305A1 (en) | 2014-08-28 |
| IN2014DN06899A (enrdf_load_stackoverflow) | 2015-05-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2010347764B2 (en) | Method for recognition and quantification of multiple analytes in a single analysis | |
| US10345293B2 (en) | High sensitivity quantitation of peptides by mass spectrometry | |
| US8455202B2 (en) | Affinity selector based recognition and quantification system and method for multiple analytes in a single analysis | |
| CN100558879C (zh) | 用质谱法高灵敏度定量肽 | |
| EP0835446B1 (en) | High speed, automated, continuous flow, multi-dimensional molecular selection and analysis | |
| EP2286237B1 (en) | Mass spectrometric analysis | |
| CA2863635A1 (en) | Selector based recognition and quantification system and method for multiple analytes in a single analysis | |
| JP7668411B2 (ja) | 無傷のタンパク質レベルでのLC-MSに基づくHbA1c測定の自動化試料ワークフロー | |
| US20150105280A1 (en) | Selector based recognition and quantification system and method for multiple analytes in a single analysis | |
| US20080090298A1 (en) | Method and system for identification of protein-protein interaction | |
| Liu et al. | The use of a quantitative cysteinyl-peptide enrichment technology for high-throughput quantitative proteomics | |
| Ohlson et al. | Emerging technologies for fragment screening | |
| EP1048950A1 (en) | Identification of ligands for orphan receptors using on-line coupling of mass spectrometry to continuous-flow separation techniques | |
| EP1300679A2 (en) | Molecular selection and analysis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |
Effective date: 20151222 |