CA2862844A1 - Methods and compositions for generating complex trait loci - Google Patents

Methods and compositions for generating complex trait loci Download PDF

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Publication number
CA2862844A1
CA2862844A1 CA2862844A CA2862844A CA2862844A1 CA 2862844 A1 CA2862844 A1 CA 2862844A1 CA 2862844 A CA2862844 A CA 2862844A CA 2862844 A CA2862844 A CA 2862844A CA 2862844 A1 CA2862844 A1 CA 2862844A1
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CA
Canada
Prior art keywords
plant
target site
transgenic target
site
interest
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2862844A
Other languages
English (en)
French (fr)
Inventor
Saverio Carl Falco
Michael Lassner
Zhongsen Li
Christopher J. Scelonge
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Hi Bred International Inc
EIDP Inc
Original Assignee
Pioneer Hi Bred International Inc
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred International Inc, EI Du Pont de Nemours and Co filed Critical Pioneer Hi Bred International Inc
Publication of CA2862844A1 publication Critical patent/CA2862844A1/en
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Physiology (AREA)
CA2862844A 2012-01-27 2013-01-24 Methods and compositions for generating complex trait loci Abandoned CA2862844A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261591329P 2012-01-27 2012-01-27
US61/591,329 2012-01-27
PCT/US2013/022891 WO2013112686A1 (en) 2012-01-27 2013-01-24 Methods and compositions for generating complex trait loci

Publications (1)

Publication Number Publication Date
CA2862844A1 true CA2862844A1 (en) 2013-08-01

Family

ID=47633591

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2862844A Abandoned CA2862844A1 (en) 2012-01-27 2013-01-24 Methods and compositions for generating complex trait loci

Country Status (10)

Country Link
US (4) US10435699B2 (https=)
EP (1) EP2807257A1 (https=)
CN (1) CN104245939A (https=)
AR (1) AR089793A1 (https=)
BR (1) BR112014018560A8 (https=)
CA (1) CA2862844A1 (https=)
EA (1) EA201491431A1 (https=)
MX (1) MX2014009023A (https=)
WO (1) WO2013112686A1 (https=)
ZA (1) ZA201405779B (https=)

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CN114736275A (zh) 2014-10-16 2022-07-12 先锋国际良种公司 具有改进的活性谱的杀昆虫多肽及其用途
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WO2017079026A1 (en) * 2015-11-06 2017-05-11 E. I. Du Pont De Nemours And Company Generation of complex trait loci in soybean and methods of use
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MX2018014993A (es) 2016-06-14 2019-09-06 Pioneer Hi Bred Int Uso de endonucleasa cpf1 para modificaciones de genoma de planta.
US20200024610A1 (en) * 2016-09-30 2020-01-23 Monsanto Technology Llc Method for selecting target sites for site-specific genome modification in plants
JP7467119B2 (ja) * 2017-02-17 2024-04-15 ロンザ リミテッド 発現困難タンパク質のための多部位ｓｓｉ細胞
WO2019165168A1 (en) 2018-02-23 2019-08-29 Pioneer Hi-Bred International, Inc. Novel cas9 orthologs
US11332752B2 (en) 2018-03-12 2022-05-17 Pioneer Hi-Bred International, Inc. Use of morphogenic factors for the improvement of gene editing
WO2020005667A1 (en) 2018-06-29 2020-01-02 Pioneer Hi-Bred International, Inc. Compositions and methods for editing an endogenous nac gene in plants
WO2020123887A2 (en) 2018-12-14 2020-06-18 Pioneer Hi-Bred International, Inc. Novel crispr-cas systems for genome editing
CN111118058B (zh) * 2020-01-09 2022-07-22 中国科学院华南植物园 用于重组酶介导的特异性位点的基因叠加的籼稻目标系
US20230091338A1 (en) 2020-02-24 2023-03-23 Pioneer Hi-Bred International, Inc. Intra-genomic homologous recombination
US20250027097A1 (en) 2021-11-30 2025-01-23 Pioneer Hi-Bred International, Inc. High efficiency large scale chromosomal genome manipulation
AU2024239266A1 (en) 2023-03-20 2025-09-25 Pioneer Hi-Bred International, Inc. Cas polypeptides with altered pam recognition

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Also Published As

Publication number Publication date
US10435699B2 (en) 2019-10-08
ZA201405779B (en) 2015-07-29
US20240093214A1 (en) 2024-03-21
AR089793A1 (es) 2014-09-17
US20190376073A1 (en) 2019-12-12
BR112014018560A8 (pt) 2017-07-11
BR112014018560A2 (https=) 2017-06-20
MX2014009023A (es) 2015-03-10
US11788097B2 (en) 2023-10-17
US20130198888A1 (en) 2013-08-01
US12146144B2 (en) 2024-11-19
US11312969B2 (en) 2022-04-26
WO2013112686A1 (en) 2013-08-01
EP2807257A1 (en) 2014-12-03
EA201491431A1 (ru) 2015-01-30
US20220251586A1 (en) 2022-08-11
CN104245939A (zh) 2014-12-24

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