CA2858252A1 - Variant alpha-amylases and methods of use, thereof - Google Patents
Variant alpha-amylases and methods of use, thereof Download PDFInfo
- Publication number
- CA2858252A1 CA2858252A1 CA2858252A CA2858252A CA2858252A1 CA 2858252 A1 CA2858252 A1 CA 2858252A1 CA 2858252 A CA2858252 A CA 2858252A CA 2858252 A CA2858252 A CA 2858252A CA 2858252 A1 CA2858252 A1 CA 2858252A1
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- Prior art keywords
- amylase
- variant
- amino acid
- composition
- detergent
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- 229920001220 nitrocellulos Polymers 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000001048 orange dye Substances 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000006072 paste Substances 0.000 description 1
- 108010087558 pectate lyase Proteins 0.000 description 1
- 210000002824 peroxisome Anatomy 0.000 description 1
- 125000000864 peroxy group Chemical group O(O*)* 0.000 description 1
- 125000005342 perphosphate group Chemical group 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- HXITXNWTGFUOAU-UHFFFAOYSA-N phenylboronic acid Chemical class OB(O)C1=CC=CC=C1 HXITXNWTGFUOAU-UHFFFAOYSA-N 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920002006 poly(N-vinylimidazole) polymer Polymers 0.000 description 1
- 239000004584 polyacrylic acid Substances 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000001205 polyphosphate Substances 0.000 description 1
- 235000011176 polyphosphates Nutrition 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229960003975 potassium Drugs 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 239000001508 potassium citrate Substances 0.000 description 1
- 229960002635 potassium citrate Drugs 0.000 description 1
- QEEAPRPFLLJWCF-UHFFFAOYSA-K potassium citrate (anhydrous) Chemical compound [K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QEEAPRPFLLJWCF-UHFFFAOYSA-K 0.000 description 1
- 235000011082 potassium citrates Nutrition 0.000 description 1
- 159000000001 potassium salts Chemical class 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 150000003138 primary alcohols Chemical class 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000004405 propyl p-hydroxybenzoate Substances 0.000 description 1
- 235000010232 propyl p-hydroxybenzoate Nutrition 0.000 description 1
- 229960003415 propylparaben Drugs 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 210000001995 reticulocyte Anatomy 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 239000003352 sequestering agent Substances 0.000 description 1
- 230000009919 sequestration Effects 0.000 description 1
- 239000013605 shuttle vector Substances 0.000 description 1
- 239000004296 sodium metabisulphite Substances 0.000 description 1
- 239000004328 sodium tetraborate Substances 0.000 description 1
- QUCDWLYKDRVKMI-UHFFFAOYSA-M sodium;3,4-dimethylbenzenesulfonate Chemical compound [Na+].CC1=CC=C(S([O-])(=O)=O)C=C1C QUCDWLYKDRVKMI-UHFFFAOYSA-M 0.000 description 1
- UIIMBOGNXHQVGW-UHFFFAOYSA-N sodium;hydron;carbonate Chemical compound [Na+].OC(O)=O UIIMBOGNXHQVGW-UHFFFAOYSA-N 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 150000003890 succinate salts Chemical class 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 150000003457 sulfones Chemical class 0.000 description 1
- 239000012209 synthetic fiber Substances 0.000 description 1
- 229920002994 synthetic fiber Polymers 0.000 description 1
- 235000013616 tea Nutrition 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- HLZKNKRTKFSKGZ-UHFFFAOYSA-N tetradecan-1-ol Chemical compound CCCCCCCCCCCCCCO HLZKNKRTKFSKGZ-UHFFFAOYSA-N 0.000 description 1
- 150000004685 tetrahydrates Chemical class 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical class [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 108700043117 vasectrin I Proteins 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 208000025247 virus-associated trichodysplasia spinulosa Diseases 0.000 description 1
- 235000014101 wine Nutrition 0.000 description 1
- 101150110790 xylB gene Proteins 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
- 235000009529 zinc sulphate Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38618—Protease or amylase in liquid compositions only
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01001—Alpha-amylase (3.2.1.1)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Detergent Compositions (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161579356P | 2011-12-22 | 2011-12-22 | |
| US61/579,356 | 2011-12-22 | ||
| PCT/US2012/070334 WO2013096305A1 (en) | 2011-12-22 | 2012-12-18 | Variant alpha-amylases and methods of use, thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2858252A1 true CA2858252A1 (en) | 2013-06-27 |
Family
ID=47436284
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2858252A Abandoned CA2858252A1 (en) | 2011-12-22 | 2012-12-18 | Variant alpha-amylases and methods of use, thereof |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20140342431A1 (OSRAM) |
| EP (1) | EP2794867A1 (OSRAM) |
| CN (1) | CN104066838A (OSRAM) |
| BR (1) | BR112014015421A2 (OSRAM) |
| CA (1) | CA2858252A1 (OSRAM) |
| IN (1) | IN2014DN03298A (OSRAM) |
| WO (1) | WO2013096305A1 (OSRAM) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2909509T3 (es) | 2012-06-08 | 2022-05-06 | Danisco Us Inc | Variante de alfa-amilasas con mayor actividad en polímeros de almidón |
| WO2016201069A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc | Low-density enzyme-containing particles |
| WO2024137250A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same |
| WO2024137248A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Compositions comprising arabinofuranosidases and a xylanase, and use thereof for increasing hemicellulosic fiber solubilization |
| CN120380158A (zh) | 2022-12-19 | 2025-07-25 | 诺维信公司 | 用于降低发酵产物生产方法后端的浆体粘度的方法 |
| CN120380140A (zh) | 2022-12-19 | 2025-07-25 | 诺维信公司 | 具有阿魏酸酯酶和/或乙酰木聚糖酯酶活性的碳水化合物酯酶家族1（ce1）多肽和编码其的多核苷酸 |
| CN115960870A (zh) * | 2022-12-22 | 2023-04-14 | 福州三合元生物科技有限公司 | 提高淀粉酶热稳定性的方法 |
| WO2025128568A1 (en) | 2023-12-11 | 2025-06-19 | Novozymes A/S | Composition and use thereof for increasing hemicellulosic fiber solubilization |
| CN118222546B (zh) * | 2024-05-27 | 2024-07-19 | 潍坊康地恩生物科技有限公司 | 一种淀粉酶突变体及其应用 |
Family Cites Families (71)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1372034A (en) | 1970-12-31 | 1974-10-30 | Unilever Ltd | Detergent compositions |
| GB1483591A (en) | 1973-07-23 | 1977-08-24 | Novo Industri As | Process for coating water soluble or water dispersible particles by means of the fluid bed technique |
| GB1590432A (en) | 1976-07-07 | 1981-06-03 | Novo Industri As | Process for the production of an enzyme granulate and the enzyme granuate thus produced |
| DK187280A (da) | 1980-04-30 | 1981-10-31 | Novo Industri As | Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode |
| DK263584D0 (da) | 1984-05-29 | 1984-05-29 | Novo Industri As | Enzymholdige granulater anvendt som detergentadditiver |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| DK171161B1 (da) | 1985-03-28 | 1996-07-08 | Hoffmann La Roche | Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve |
| EP0218272B1 (en) | 1985-08-09 | 1992-03-18 | Gist-Brocades N.V. | Novel lipolytic enzymes and their use in detergent compositions |
| EG18543A (en) | 1986-02-20 | 1993-07-30 | Albright & Wilson | Protected enzyme systems |
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| ES2058119T3 (es) | 1986-08-29 | 1994-11-01 | Novo Nordisk As | Aditivo detergente enzimatico. |
| NZ221627A (en) | 1986-09-09 | 1993-04-28 | Genencor Inc | Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios |
| DE3854249T2 (de) | 1987-08-28 | 1996-02-29 | Novonordisk As | Rekombinante Humicola-Lipase und Verfahren zur Herstellung von rekombinanten Humicola-Lipasen. |
| JPS6474992A (en) | 1987-09-16 | 1989-03-20 | Fuji Oil Co Ltd | Dna sequence, plasmid and production of lipase |
| DK6488D0 (da) | 1988-01-07 | 1988-01-07 | Novo Industri As | Enzymer |
| EP0471265B1 (en) | 1988-01-07 | 1995-10-25 | Novo Nordisk A/S | Specific protease |
| JP3079276B2 (ja) | 1988-02-28 | 2000-08-21 | 天野製薬株式会社 | 組換え体ｄｎａ、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法 |
| EP0406314B1 (en) | 1988-03-24 | 1993-12-01 | Novo Nordisk A/S | A cellulase preparation |
| US5648263A (en) | 1988-03-24 | 1997-07-15 | Novo Nordisk A/S | Methods for reducing the harshness of a cotton-containing fabric |
| GB8915658D0 (en) | 1989-07-07 | 1989-08-23 | Unilever Plc | Enzymes,their production and use |
| ATE107355T1 (de) | 1990-04-14 | 1994-07-15 | Kali Chemie Ag | Alkalische bacillus-lipasen, hierfür codierende dna-sequenzen sowie bacilli, die diese lipasen produzieren. |
| DK115890D0 (da) | 1990-05-09 | 1990-05-09 | Novo Nordisk As | Enzym |
| WO1991017243A1 (en) | 1990-05-09 | 1991-11-14 | Novo Nordisk A/S | A cellulase preparation comprising an endoglucanase enzyme |
| ES2121786T3 (es) | 1990-09-13 | 1998-12-16 | Novo Nordisk As | Variantes de lipasa. |
| SK46293A3 (en) | 1990-09-28 | 1994-01-12 | Procter & Gamble | Polyhydroxy fatty acid amide surfactants to enhance enzyme performance |
| EP0495258A1 (en) | 1991-01-16 | 1992-07-22 | The Procter & Gamble Company | Detergent compositions with high activity cellulase and softening clays |
| EP0511456A1 (en) | 1991-04-30 | 1992-11-04 | The Procter & Gamble Company | Liquid detergents with aromatic borate ester to inhibit proteolytic enzyme |
| AU666660B2 (en) | 1991-04-30 | 1996-02-22 | Procter & Gamble Company, The | Built liquid detergents with boric-polyol complex to inhibit proteolytic enzyme |
| JP3471797B2 (ja) | 1991-05-01 | 2003-12-02 | ノボザイムス アクティーゼルスカブ | 安定化酵素及び洗剤 |
| DK72992D0 (da) | 1992-06-01 | 1992-06-01 | Novo Nordisk As | Enzym |
| DK88892D0 (da) | 1992-07-06 | 1992-07-06 | Novo Nordisk As | Forbindelse |
| US5792641A (en) | 1992-10-06 | 1998-08-11 | Novo Nordisk A/S | Cellulase variants and detergent compositions containing cellulase variants |
| US5281526A (en) | 1992-10-20 | 1994-01-25 | Solvay Enzymes, Inc. | Method of purification of amylase by precipitation with a metal halide and 4-hydroxybenzic acid or a derivative thereof |
| DE69434242T2 (de) | 1993-04-27 | 2006-01-12 | Genencor International, Inc., Palo Alto | Neuartige Lipasevarianten zur Verwendung in Reinigungsmitteln |
| DK52393D0 (OSRAM) | 1993-05-05 | 1993-05-05 | Novo Nordisk As | |
| JP2859520B2 (ja) | 1993-08-30 | 1999-02-17 | ノボ ノルディスク アクティーゼルスカブ | リパーゼ及びそれを生産する微生物及びリパーゼ製造方法及びリパーゼ含有洗剤組成物 |
| WO1995010602A1 (en) | 1993-10-13 | 1995-04-20 | Novo Nordisk A/S | H2o2-stable peroxidase variants |
| JPH07143883A (ja) | 1993-11-24 | 1995-06-06 | Showa Denko Kk | リパーゼ遺伝子及び変異体リパーゼ |
| KR970701264A (ko) | 1994-02-22 | 1997-03-17 | 안네 제케르 | 지질분해효소의 변이체 제조방법(a method of preparing a viriant of a lipolytic enzyme) |
| EP1632557B1 (en) | 1994-03-08 | 2011-02-23 | Novozymes A/S | Novel alkaline cellulases |
| WO1995030744A2 (en) | 1994-05-04 | 1995-11-16 | Genencor International Inc. | Lipases with improved surfactant resistance |
| AU2884595A (en) | 1994-06-20 | 1996-01-15 | Unilever Plc | Modified pseudomonas lipases and their use |
| AU2884695A (en) | 1994-06-23 | 1996-01-19 | Unilever Plc | Modified pseudomonas lipases and their use |
| EP0788541B1 (en) | 1994-10-06 | 2008-03-12 | Novozymes A/S | Enzyme preparation with endoglucanase activity |
| BE1008998A3 (fr) | 1994-10-14 | 1996-10-01 | Solvay | Lipase, microorganisme la produisant, procede de preparation de cette lipase et utilisations de celle-ci. |
| US5827719A (en) | 1994-10-26 | 1998-10-27 | Novo Nordisk A/S | Enzyme with lipolytic activity |
| JPH08228778A (ja) | 1995-02-27 | 1996-09-10 | Showa Denko Kk | 新規なリパーゼ遺伝子及びそれを用いたリパーゼの製造方法 |
| DE815209T1 (de) | 1995-03-17 | 1998-06-25 | Novonordisk As | Neue endoglukanase |
| ATE282087T1 (de) | 1995-07-14 | 2004-11-15 | Novozymes As | Modifiziertes enzym mit lipolytischer aktivität |
| JP4068142B2 (ja) | 1995-08-11 | 2008-03-26 | ノボザイムス アクティーゼルスカブ | 新規の脂肪分解酵素 |
| AU3938997A (en) | 1996-08-26 | 1998-03-19 | Novo Nordisk A/S | A novel endoglucanase |
| CN100362100C (zh) | 1996-09-17 | 2008-01-16 | 诺沃奇梅兹有限公司 | 纤维素酶变体 |
| AU730286B2 (en) | 1996-10-08 | 2001-03-01 | Novo Nordisk A/S | Diaminobenzoic acid derivatives as dye precursors |
| EP0932667B1 (en) | 1996-11-04 | 2008-10-01 | Novozymes A/S | Subtilase variants and compositions |
| JP4044143B2 (ja) | 1996-11-04 | 2008-02-06 | ノボザイムス アクティーゼルスカブ | ズブチラーゼ変異体及び組成物 |
| WO1998034946A1 (en) | 1997-02-12 | 1998-08-13 | Massachusetts Institute Of Technology | Daxx, a novel fas-binding protein that activates jnk and apoptosis |
| DE19736591A1 (de) | 1997-08-22 | 1999-02-25 | Peter Prof Dr Hegemann | Verfahren zum Herstellen von Nukleinsäurepolymeren |
| US6254645B1 (en) | 1999-08-20 | 2001-07-03 | Genencor International, Inc. | Enzymatic modification of the surface of a polyester fiber or article |
| US6933140B1 (en) | 1999-11-05 | 2005-08-23 | Genencor International, Inc. | Enzymes useful for changing the properties of polyester |
| EP1309677B2 (en) | 2000-08-11 | 2012-04-11 | Genencor International, Inc. | Bacillus transformation, transformants and mutant libraries |
| DE10260805A1 (de) | 2002-12-23 | 2004-07-22 | Geneart Gmbh | Verfahren und Vorrichtung zum Optimieren einer Nucleotidsequenz zur Expression eines Proteins |
| US7754460B2 (en) | 2003-12-03 | 2010-07-13 | Danisco Us Inc. | Enzyme for the production of long chain peracid |
| CA2547709C (en) | 2003-12-03 | 2017-02-07 | Genencor International, Inc. | Perhydrolase |
| US8703459B2 (en) | 2003-12-05 | 2014-04-22 | The United States Of America, As Represented By The Secretary, Department Of Health & Human Services | Catalytic domains of beta(1,4)-galactosyltransferase I having altered metal ion specificity |
| US7723083B2 (en) | 2005-12-13 | 2010-05-25 | E.I. Du Pont De Nemours And Company | Production of peracids using an enzyme having perhydrolysis activity |
| US7951566B2 (en) | 2005-12-13 | 2011-05-31 | E.I. Du Pont De Nemours And Company | Production of peracids using an enzyme having perhydrolysis activity |
| EP2215202B2 (en) | 2007-11-05 | 2024-01-10 | Danisco US Inc. | VARIANTS OF BACILLUS sp. TS-23 ALPHA-AMYLASE WITH ALTERED PROPERTIES |
| WO2009100102A2 (en) * | 2008-02-04 | 2009-08-13 | Danisco Us Inc., Genencor Division | Ts23 alpha-amylase variants with altered properties |
| WO2010039958A1 (en) | 2008-10-03 | 2010-04-08 | E.I. Dupont De Nemours And Company | Improved perhydrolases for enzymatic peracid generation |
| JP5651682B2 (ja) | 2009-04-01 | 2015-01-14 | ダニスコ・ユーエス・インク | 変化した特性を有するα−アミラーゼ変異体を含む組成物及び方法 |
-
2012
- 2012-12-18 CA CA2858252A patent/CA2858252A1/en not_active Abandoned
- 2012-12-18 US US14/364,408 patent/US20140342431A1/en not_active Abandoned
- 2012-12-18 EP EP12806851.7A patent/EP2794867A1/en not_active Withdrawn
- 2012-12-18 BR BR112014015421A patent/BR112014015421A2/pt not_active IP Right Cessation
- 2012-12-18 WO PCT/US2012/070334 patent/WO2013096305A1/en not_active Ceased
- 2012-12-18 CN CN201280063219.5A patent/CN104066838A/zh active Pending
- 2012-12-18 IN IN3298DEN2014 patent/IN2014DN03298A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BR112014015421A2 (pt) | 2019-09-24 |
| US20140342431A1 (en) | 2014-11-20 |
| IN2014DN03298A (OSRAM) | 2015-06-26 |
| CN104066838A (zh) | 2014-09-24 |
| EP2794867A1 (en) | 2014-10-29 |
| WO2013096305A1 (en) | 2013-06-27 |
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