CA2853711C - A liquid enzyme formulation and a process for its preparation - Google Patents

A liquid enzyme formulation and a process for its preparation Download PDF

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Publication number
CA2853711C
CA2853711C CA2853711A CA2853711A CA2853711C CA 2853711 C CA2853711 C CA 2853711C CA 2853711 A CA2853711 A CA 2853711A CA 2853711 A CA2853711 A CA 2853711A CA 2853711 C CA2853711 C CA 2853711C
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Prior art keywords
transglutaminase
formulation
polyol
enzyme
suspension
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CA2853711A
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English (en)
French (fr)
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CA2853711A1 (en
Inventor
Kirsi Rajakari
Kai Hotakainen
Paivi Myllarinen
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Valio Oy
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
    • C12N9/104Aminoacyltransferases (2.3.2)
    • C12N9/1044Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06Enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0055Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0059Catechol oxidase (1.10.3.1), i.e. tyrosinase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0055Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061Laccase (1.10.3.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/78Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y110/00Oxidoreductases acting on diphenols and related substances as donors (1.10)
    • C12Y110/03Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
    • C12Y110/03001Catechol oxidase (1.10.3.1), i.e. tyrosinase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y110/00Oxidoreductases acting on diphenols and related substances as donors (1.10)
    • C12Y110/03Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
    • C12Y110/03002Laccase (1.10.3.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y203/00Acyltransferases (2.3)
    • C12Y203/02Aminoacyltransferases (2.3.2)
    • C12Y203/02013Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y305/00Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01043Peptidyl-glutaminase (3.5.1.43)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
CA2853711A 2011-11-01 2012-10-31 A liquid enzyme formulation and a process for its preparation Active CA2853711C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FI20116074A FI126442B (en) 2011-11-01 2011-11-01 Liquid enzyme preparation and process for its preparation
FI20116074 2011-11-01
PCT/FI2012/051048 WO2013064736A1 (en) 2011-11-01 2012-10-31 A liquid enzyme formulation and a process for its preparation

Publications (2)

Publication Number Publication Date
CA2853711A1 CA2853711A1 (en) 2013-05-10
CA2853711C true CA2853711C (en) 2019-11-26

Family

ID=47215584

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2853711A Active CA2853711C (en) 2011-11-01 2012-10-31 A liquid enzyme formulation and a process for its preparation

Country Status (19)

Country Link
US (1) US20140287098A1 (enExample)
EP (1) EP2773753B1 (enExample)
JP (1) JP6297979B2 (enExample)
KR (1) KR101998182B1 (enExample)
CN (2) CN104024406A (enExample)
AU (1) AU2012331034B2 (enExample)
BR (1) BR112014010222B1 (enExample)
CA (1) CA2853711C (enExample)
CL (1) CL2014001100A1 (enExample)
DK (1) DK2773753T3 (enExample)
ES (1) ES2697675T3 (enExample)
FI (1) FI126442B (enExample)
IL (1) IL232356B (enExample)
IN (1) IN2014MN00968A (enExample)
MX (1) MX356454B (enExample)
PL (1) PL2773753T3 (enExample)
RU (1) RU2668396C2 (enExample)
WO (1) WO2013064736A1 (enExample)
ZA (1) ZA201403965B (enExample)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2913045B1 (en) 2012-10-17 2019-04-10 Ajinomoto Co., Inc. Cosmetic composition
CN107177582B (zh) * 2016-02-16 2018-05-22 上海青瑞食品科技有限公司 一种液体酶制剂及制备方法
SG11201808571XA (en) 2016-04-01 2018-10-30 Oatly Ab Enhanced viscosity oat base and fermented oat base product
CN105852053A (zh) * 2016-04-20 2016-08-17 上海青瑞食品科技有限公司 一种液体酶制剂在食品质构改良中的应用
JP6787563B2 (ja) * 2016-06-30 2020-11-18 千葉製粉株式会社 接着成形食品の製造方法、接着成形食品用の接着成分分散液、および、接着成形食品
CN107897533A (zh) * 2017-12-10 2018-04-13 济南诺能生物工程有限公司 一种单胃动物液体复合酶的制备方法
JP7501357B2 (ja) 2018-03-23 2024-06-18 味の素株式会社 トランスグルタミナーゼを含有する液体製剤
CA3171481A1 (en) * 2020-03-17 2021-09-23 Kiyota SAKAI Protein crosslinking method
US20230203457A1 (en) * 2020-05-13 2023-06-29 Curie Co. Inc. Transglutaminase variants and applications of use thereof
EP4212626A4 (en) * 2020-09-10 2024-05-15 Amano Enzyme Inc. METHOD FOR PRODUCING PROCESSED PROTEIN
US20230329292A1 (en) * 2020-09-10 2023-10-19 Amano Enzyme Inc. Tofu production method
JP7829496B2 (ja) * 2020-12-04 2026-03-13 天野エンザイム株式会社 液体酵素製剤
WO2023058616A1 (ja) * 2021-10-04 2023-04-13 天野エンザイム株式会社 液状トランスグルタミナーゼ酵素製剤
WO2023228953A1 (ja) * 2022-05-23 2023-11-30 天野エンザイム株式会社 液体酵素製剤
CN115380981A (zh) * 2022-08-09 2022-11-25 武汉新华扬生物股份有限公司 一种用于冷萃咖啡的复配酶制剂及其应用方法
EP4428218A1 (en) * 2023-03-07 2024-09-11 SkyLab AG Biodegradable transglutaminase-based detergent composition for regeneration of microdamage and regulation of elasticity of delicate fiber
CN121285627A (zh) 2023-06-14 2026-01-06 天野酶制品株式会社 液体酶制剂
CN121569032A (zh) 2023-09-01 2026-02-24 诺维信公司 稳定的液体脱酰胺酶组合物
CN121666449A (zh) * 2023-09-13 2026-03-13 诺维信公司 稳定的脱酰胺酶组合物
WO2025233391A1 (en) * 2024-05-09 2025-11-13 Novozymes A/S Stabilized liquid deamidase compositions
EP4650443A1 (en) 2024-05-13 2025-11-19 Novozymes A/S Stabilized liquid deamidase compositions

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0665280B2 (ja) 1987-03-04 1994-08-24 味の素株式会社 タンパクゲル化剤及びそれを用いるタンパクのゲル化方法
US5340734A (en) * 1992-11-25 1994-08-23 Biosource Genetics Corporation Method for making stable, extracellular tyrosinase and synthesis of polyphenolic polymers therefrom
CA2165157C (en) * 1994-04-18 2007-06-12 Luppo Edens Stable water-in-oil emulsions
DE69535037T2 (de) 1994-08-26 2006-12-07 Novozymes A/S Mikrobielle transglutaminasen, ihre herstellung und ihre verwendung
ATE330004T1 (de) * 1997-03-12 2006-07-15 Novozymes As Lagerungsstabile, flüssige zusammensetzung, welche laccase enthalten
PT1331850E (pt) * 2000-09-28 2007-03-30 Dsm Ip Assets Bv Composições líquidas de melhoramento de pão
US20030219853A1 (en) * 2002-03-01 2003-11-27 Szu-Yi Chou Method of cross-linking a compound
ES2441414T3 (es) * 2002-09-26 2014-02-04 Ajinomoto Co., Inc. Preparación enzimática y procedimiento para producir alimentos que utiliza la misma
JP2005034078A (ja) * 2003-07-17 2005-02-10 Rengo Co Ltd タンパク質安定化剤
EP1836897A1 (en) * 2006-03-22 2007-09-26 Nestec S.A. A solid product comprising oil-droplets
GB2438287A (en) * 2006-05-19 2007-11-21 Norbrook Lab Ltd Stable aqueous suspension
EP2251421B1 (en) * 2008-02-13 2016-11-16 Amano Enzyme Inc. Stabilized transglutaminase and process for production thereof
US20110064847A1 (en) * 2008-03-14 2011-03-17 Ajinomoto Co., Inc. Method of denaturing protein with enzymes
US20110189344A1 (en) * 2008-06-09 2011-08-04 Michael Bodo Recovery of insoluble enzyme from fermentation broth and formulation of insoluble enzyme

Also Published As

Publication number Publication date
EP2773753B1 (en) 2018-10-17
BR112014010222A8 (pt) 2017-06-20
JP6297979B2 (ja) 2018-03-20
IL232356A0 (en) 2014-06-30
CL2014001100A1 (es) 2014-09-05
US20140287098A1 (en) 2014-09-25
EP2773753A1 (en) 2014-09-10
KR20140096090A (ko) 2014-08-04
KR101998182B1 (ko) 2019-10-01
WO2013064736A1 (en) 2013-05-10
IN2014MN00968A (enExample) 2015-04-24
ES2697675T3 (es) 2019-01-25
MX2014005128A (es) 2014-07-09
CA2853711A1 (en) 2013-05-10
MX356454B (es) 2018-05-30
JP2014532421A (ja) 2014-12-08
IL232356B (en) 2019-03-31
BR112014010222B1 (pt) 2022-04-12
AU2012331034B2 (en) 2017-10-19
ZA201403965B (en) 2015-05-27
BR112014010222A2 (pt) 2017-06-13
DK2773753T3 (en) 2018-12-10
RU2668396C2 (ru) 2018-09-28
CN104024406A (zh) 2014-09-03
PL2773753T3 (pl) 2019-03-29
CN108676782A (zh) 2018-10-19
FI20116074L (fi) 2013-05-02
AU2012331034A1 (en) 2014-06-26
RU2014121318A (ru) 2015-12-10
FI126442B (en) 2016-12-15

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