CA2849303C - Dehydrogenase variants and polynucleotides encoding same - Google Patents
Dehydrogenase variants and polynucleotides encoding same Download PDFInfo
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- CA2849303C CA2849303C CA2849303A CA2849303A CA2849303C CA 2849303 C CA2849303 C CA 2849303C CA 2849303 A CA2849303 A CA 2849303A CA 2849303 A CA2849303 A CA 2849303A CA 2849303 C CA2849303 C CA 2849303C
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/52—Propionic acid; Butyric acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01059—3-Hydroxypropionate dehydrogenase (1.1.1.59)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01276—Serine 3-dehydrogenase (1.1.1.276)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01298—3-Hydroxypropionate dehydrogenase (NADP+) (1.1.1.298)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
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- Chemical & Material Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
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Applications Claiming Priority (3)
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|---|---|---|---|
| US201161541363P | 2011-09-30 | 2011-09-30 | |
| US61/541,363 | 2011-09-30 | ||
| PCT/US2012/057134 WO2013049073A1 (en) | 2011-09-30 | 2012-09-25 | Dehydrogenase variants and polynucleotides encoding same |
Publications (2)
| Publication Number | Publication Date |
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| CA2849303A1 CA2849303A1 (en) | 2013-04-04 |
| CA2849303C true CA2849303C (en) | 2019-09-17 |
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| EP (1) | EP2751261B1 (enExample) |
| JP (3) | JP2014528726A (enExample) |
| CN (1) | CN103857791B (enExample) |
| AU (1) | AU2012316246B2 (enExample) |
| BR (1) | BR112014007647A2 (enExample) |
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| DK (1) | DK2751261T3 (enExample) |
| IN (1) | IN2014CN03187A (enExample) |
| WO (1) | WO2013049073A1 (enExample) |
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| EP2751261B1 (en) * | 2011-09-30 | 2018-08-29 | Novozymes, Inc. | Dehydrogenase variants and polynucleotides encoding same |
| BR112016001778A2 (pt) | 2013-07-31 | 2017-09-05 | Novozymes As | Célula transgênica de levedura, composição, e, métodos de produção de 3-hp e de produção de ácido acrílico ou um sal do mesmo |
| CN107109347A (zh) * | 2014-12-19 | 2017-08-29 | 诺维信公司 | 用于生产3‑羟基丙酸的重组宿主细胞 |
| WO2016154046A2 (en) | 2015-03-20 | 2016-09-29 | Verdezyne, Inc. | Biological methods for preparing 3-hydroxypropionic acid |
| EP3341475A1 (en) | 2015-08-24 | 2018-07-04 | Novozymes A/S | Beta-alanine aminotransferases for the production of 3-hydroxypropionic acid |
| WO2023168233A1 (en) * | 2022-03-03 | 2023-09-07 | Cargill, Incorporated | Genetically modified yeast and fermentation processes for the production of 3-hydroxypropionate |
| WO2023168244A1 (en) * | 2022-03-03 | 2023-09-07 | Cargill, Incorporated | Genetically modified yeast and fermentation processes for the production of 3-hydroxypropionate |
| KR102782737B1 (ko) * | 2022-11-29 | 2025-03-19 | 씨제이제일제당 주식회사 | 신규한 프로피온알데하이드 디하이드로게나제 활성을 갖는 변이체 폴리펩티드 및 이의 용도 |
Family Cites Families (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| NZ237549A (en) | 1990-03-23 | 1993-06-25 | Gist Brocades Nv | Production of enhanced levels of enzymes in the seeds of transgenic plants and the use of these seeds |
| US6395966B1 (en) | 1990-08-09 | 2002-05-28 | Dekalb Genetics Corp. | Fertile transgenic maize plants containing a gene encoding the pat protein |
| IL99552A0 (en) | 1990-09-28 | 1992-08-18 | Ixsys Inc | Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof |
| FR2704860B1 (fr) | 1993-05-05 | 1995-07-13 | Pasteur Institut | Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire. |
| DE4343591A1 (de) | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| KR970703426A (ko) | 1994-06-03 | 1997-07-03 | 제임스 쉐한 | 정제된 Myceliophthora 락카제 및 그것을 암호화 하는 핵산(PURIFIED MYCELIOPHTHORA LACCASES AND NUCLEIC ACIDS ENCODING SAME) |
| EP0777737B1 (en) | 1994-06-30 | 2005-05-04 | Novozymes Biotech, Inc. | Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein |
| US5955310A (en) | 1998-02-26 | 1999-09-21 | Novo Nordisk Biotech, Inc. | Methods for producing a polypeptide in a bacillus cell |
| JP5043254B2 (ja) | 1998-10-26 | 2012-10-10 | ノボザイムス アクティーゼルスカブ | 糸状面細胞内の問題のｄｎａライブラリーの作製及びスクリーニング |
| AU4025700A (en) | 1999-03-22 | 2000-10-09 | Novo Nordisk Biotech, Inc. | Promoters for expressing genes in a fungal cell |
| WO2001016346A1 (en) | 1999-08-30 | 2001-03-08 | Wisconsin Alumni Research Foundation | Production of 3-hydroxypropionic acid in recombinant organisms |
| AR031480A1 (es) | 2000-11-20 | 2003-09-24 | Cargill Inc | Acido 3-hidroxipropionico y otros compuestos organicos |
| US7151204B2 (en) | 2001-01-09 | 2006-12-19 | Monsanto Technology Llc | Maize chloroplast aldolase promoter compositions and methods for use thereof |
| ATE375388T1 (de) | 2001-07-27 | 2007-10-15 | Us Gov Health & Human Serv | Systeme zur stellengerichteten in-vivo-mutagenese mit oligonukleotiden |
| US7309597B2 (en) | 2002-01-18 | 2007-12-18 | Cargill, Incorporated | Alanine 2,3-Aminomutase |
| CA2480152A1 (en) | 2002-03-25 | 2003-10-09 | Cargill, Incorporated | Methods of manufacturing derivatives of .beta.-hydroxycarboxylic acids |
| EP1706457B1 (en) | 2003-12-04 | 2012-02-01 | Novozymes A/S | Production of 3-hydroxypropionic acid using beta-alanine/pyruvate aminotransferase |
| JP2008507970A (ja) | 2004-07-30 | 2008-03-21 | カーギル インコーポレイテッド | アラニン２，３アミノムターゼ |
| JP2008517607A (ja) | 2004-10-25 | 2008-05-29 | コデクシス， インコーポレイテッド | 改良されたアラニン２，３−アミノムターゼおよび関連ポリヌクレオチド |
| DE102005048818A1 (de) | 2005-10-10 | 2007-04-12 | Degussa Ag | Mikrobiologische Herstellung von 3-Hydroxypropionsäure |
| BRPI0716212A2 (pt) | 2006-08-30 | 2013-10-15 | Cargill Inc | Beta-alanina/alfa-cetoglutarato aminotransferase para produção de ácido 3-hidroxipropiônico |
| CA2675026A1 (en) | 2007-01-12 | 2008-07-24 | The Regents Of The University Of Colorado, A Body Corporate | Compositions and methods for enhancing tolerance for the production of organic chemicals produced by microorganisms |
| US8673601B2 (en) | 2007-01-22 | 2014-03-18 | Genomatica, Inc. | Methods and organisms for growth-coupled production of 3-hydroxypropionic acid |
| WO2009089457A1 (en) | 2008-01-11 | 2009-07-16 | Novozymes A/S | Methods for producing 3-hydroxypropionic acid and compounds thereof |
| US20100021978A1 (en) | 2008-07-23 | 2010-01-28 | Genomatica, Inc. | Methods and organisms for production of 3-hydroxypropionic acid |
| EP2326709A4 (en) | 2008-09-15 | 2013-01-23 | Opx Biotechnologies Inc | METHODS, SYSTEMS AND COMPOSITIONS RELATED TO REDUCED MICROBIAL PRODUCTION OF 3-HYDROXYPROPIONIC ACID (3-HP) IN ALDEHYDMETABOLITE |
| KR101344695B1 (ko) | 2008-12-17 | 2013-12-26 | 생-고뱅 세라믹스 앤드 플라스틱스, 인코포레이티드 | 전극 가스 채널 지지체, 및 내부 채널을 형성하는 방법 |
| KR20140015136A (ko) * | 2009-09-27 | 2014-02-06 | 더 리젠츠 오브 더 유니버시티 오브 콜로라도, 어 바디 코포레이트 | 3-히드록시프로피온산 및 다른 생성물의 제조 방법 |
| MY159487A (en) * | 2010-02-12 | 2017-01-13 | Gevo Inc | Reduced by-product accumulation for improved production of isobutanol |
| JP2012085635A (ja) * | 2010-03-23 | 2012-05-10 | Nippon Shokubai Co Ltd | ３−ヒドロキシプロピオン酸の製造方法 |
| JP6061862B2 (ja) | 2010-11-22 | 2017-01-18 | カーギル，インコーポレイティド | ３‐ヒドロキシプロピオン酸生産用の組成物及び方法 |
| WO2013043758A2 (en) | 2011-09-19 | 2013-03-28 | Opx Biotechnologies, Inc. | Compositions and methods regarding direct nadh utilization to produce 3-hydroxypropionic acid, derived chemicals and further derived products |
| EP2751261B1 (en) * | 2011-09-30 | 2018-08-29 | Novozymes, Inc. | Dehydrogenase variants and polynucleotides encoding same |
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| US8728788B1 (en) | 2014-05-20 |
| US9163220B2 (en) | 2015-10-20 |
| EP2751261A1 (en) | 2014-07-09 |
| WO2013049073A1 (en) | 2013-04-04 |
| BR112014007647A2 (pt) | 2017-04-18 |
| CN103857791B (zh) | 2016-11-23 |
| JP2014528726A (ja) | 2014-10-30 |
| CN103857791A (zh) | 2014-06-11 |
| US9404091B2 (en) | 2016-08-02 |
| DK2751261T3 (en) | 2018-12-10 |
| US20160017296A1 (en) | 2016-01-21 |
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| IN2014CN03187A (enExample) | 2015-07-03 |
| JP2019110916A (ja) | 2019-07-11 |
| EP2751261B1 (en) | 2018-08-29 |
| US20140212938A1 (en) | 2014-07-31 |
| AU2012316246A1 (en) | 2014-03-20 |
| AU2012316246B2 (en) | 2017-10-26 |
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