CA2835053C - A method of increasing the productivity of eucaryotic cells in the production of recombinant fviii - Google Patents
A method of increasing the productivity of eucaryotic cells in the production of recombinant fviii Download PDFInfo
- Publication number
- CA2835053C CA2835053C CA2835053A CA2835053A CA2835053C CA 2835053 C CA2835053 C CA 2835053C CA 2835053 A CA2835053 A CA 2835053A CA 2835053 A CA2835053 A CA 2835053A CA 2835053 C CA2835053 C CA 2835053C
- Authority
- CA
- Canada
- Prior art keywords
- cells
- cell
- cell suspension
- rfviii
- increasing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 44
- 230000001965 increasing effect Effects 0.000 title claims abstract description 40
- 238000004519 manufacturing process Methods 0.000 title description 14
- 210000004027 cell Anatomy 0.000 claims abstract description 103
- 239000006285 cell suspension Substances 0.000 claims abstract description 40
- 238000012258 culturing Methods 0.000 claims abstract description 17
- 239000003599 detergent Substances 0.000 claims abstract description 12
- 210000003527 eukaryotic cell Anatomy 0.000 claims abstract description 12
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 11
- 239000001110 calcium chloride Substances 0.000 claims abstract description 11
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 11
- 235000011148 calcium chloride Nutrition 0.000 claims abstract description 11
- 230000001939 inductive effect Effects 0.000 claims abstract description 6
- 108010054218 Factor VIII Proteins 0.000 claims abstract description 5
- 102000001690 Factor VIII Human genes 0.000 claims abstract description 5
- 229960000301 factor viii Drugs 0.000 claims abstract description 5
- 235000015097 nutrients Nutrition 0.000 claims abstract description 4
- 230000033001 locomotion Effects 0.000 claims description 11
- 239000012510 hollow fiber Substances 0.000 claims description 9
- 239000012528 membrane Substances 0.000 claims description 8
- 239000000725 suspension Substances 0.000 claims description 7
- 210000005260 human cell Anatomy 0.000 claims description 5
- 238000005086 pumping Methods 0.000 claims description 4
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 3
- 230000002776 aggregation Effects 0.000 claims description 3
- 238000004220 aggregation Methods 0.000 claims description 3
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 3
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 3
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 3
- 229920000053 polysorbate 80 Polymers 0.000 claims description 3
- 230000005484 gravity Effects 0.000 claims description 2
- 230000003068 static effect Effects 0.000 claims description 2
- 238000003756 stirring Methods 0.000 description 28
- 101000911390 Homo sapiens Coagulation factor VIII Proteins 0.000 description 25
- 102100026735 Coagulation factor VIII Human genes 0.000 description 24
- 238000004113 cell culture Methods 0.000 description 22
- 230000010412 perfusion Effects 0.000 description 10
- 239000000126 substance Substances 0.000 description 9
- 210000004962 mammalian cell Anatomy 0.000 description 8
- 238000002156 mixing Methods 0.000 description 8
- 230000021715 photosynthesis, light harvesting Effects 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 8
- 239000002609 medium Substances 0.000 description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 6
- 230000010261 cell growth Effects 0.000 description 6
- 229910052760 oxygen Inorganic materials 0.000 description 6
- 239000001301 oxygen Substances 0.000 description 6
- -1 polyoxyethylene Polymers 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000005119 centrifugation Methods 0.000 description 5
- 239000003593 chromogenic compound Substances 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 238000012512 characterization method Methods 0.000 description 4
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 230000014616 translation Effects 0.000 description 4
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 3
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 3
- 239000006143 cell culture medium Substances 0.000 description 3
- 230000030833 cell death Effects 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 229920001983 poloxamer Polymers 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 230000035899 viability Effects 0.000 description 3
- 102000003886 Glycoproteins Human genes 0.000 description 2
- 108090000288 Glycoproteins Proteins 0.000 description 2
- 102000014150 Interferons Human genes 0.000 description 2
- 108010050904 Interferons Proteins 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- HQPMKSGTIOYHJT-UHFFFAOYSA-N ethane-1,2-diol;propane-1,2-diol Chemical compound OCCO.CC(O)CO HQPMKSGTIOYHJT-UHFFFAOYSA-N 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 210000004408 hybridoma Anatomy 0.000 description 2
- 229940079322 interferon Drugs 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 230000006461 physiological response Effects 0.000 description 2
- 229920001993 poloxamer 188 Polymers 0.000 description 2
- 229920001451 polypropylene glycol Polymers 0.000 description 2
- 229920000136 polysorbate Polymers 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000003252 repetitive effect Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- XZIIFPSPUDAGJM-UHFFFAOYSA-N 6-chloro-2-n,2-n-diethylpyrimidine-2,4-diamine Chemical class CCN(CC)C1=NC(N)=CC(Cl)=N1 XZIIFPSPUDAGJM-UHFFFAOYSA-N 0.000 description 1
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 241000699802 Cricetulus griseus Species 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 102000002265 Human Growth Hormone Human genes 0.000 description 1
- 108010000521 Human Growth Hormone Proteins 0.000 description 1
- 239000000854 Human Growth Hormone Substances 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000005775 apoptotic pathway Effects 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000012365 batch cultivation Methods 0.000 description 1
- 208000034158 bleeding Diseases 0.000 description 1
- 231100000319 bleeding Toxicity 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 239000012534 cell culture medium component Substances 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 238000004581 coalescence Methods 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 238000004042 decolorization Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- KAQKFAOMNZTLHT-VVUHWYTRSA-N epoprostenol Chemical compound O1C(=CCCCC(O)=O)C[C@@H]2[C@@H](/C=C/[C@@H](O)CCCCC)[C@H](O)C[C@@H]21 KAQKFAOMNZTLHT-VVUHWYTRSA-N 0.000 description 1
- 229960001123 epoprostenol Drugs 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 230000004153 glucose metabolism Effects 0.000 description 1
- 230000004190 glucose uptake Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 102000057593 human F8 Human genes 0.000 description 1
- 229960000900 human factor viii Drugs 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000007775 late Effects 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 230000001338 necrotic effect Effects 0.000 description 1
- 231100001160 nonlethal Toxicity 0.000 description 1
- 238000012576 optical tweezer Methods 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 230000003334 potential effect Effects 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 238000011165 process development Methods 0.000 description 1
- 230000000541 pulsatile effect Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0684—Cells of the urinary tract or kidneys
- C12N5/0686—Kidney cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2511/00—Cells for large scale production
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2521/00—Culture process characterised by the use of hydrostatic pressure, flow or shear forces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Sustainable Development (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP11166071 | 2011-05-13 | ||
| EP11166071.8 | 2011-05-13 | ||
| US201161489406P | 2011-05-24 | 2011-05-24 | |
| US61/489,406 | 2011-05-24 | ||
| PCT/EP2012/058899 WO2012156356A1 (en) | 2011-05-13 | 2012-05-14 | A method of increasing the productivity of eucaryotic cells in the production of recombinant fviii |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2835053A1 CA2835053A1 (en) | 2012-11-22 |
| CA2835053C true CA2835053C (en) | 2020-07-07 |
Family
ID=45470754
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2835053A Expired - Fee Related CA2835053C (en) | 2011-05-13 | 2012-05-14 | A method of increasing the productivity of eucaryotic cells in the production of recombinant fviii |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US9115381B2 (cg-RX-API-DMAC7.html) |
| EP (3) | EP4353743A3 (cg-RX-API-DMAC7.html) |
| JP (1) | JP6178786B2 (cg-RX-API-DMAC7.html) |
| KR (1) | KR101980164B1 (cg-RX-API-DMAC7.html) |
| CN (1) | CN103517919B (cg-RX-API-DMAC7.html) |
| AU (1) | AU2012257736B2 (cg-RX-API-DMAC7.html) |
| BR (1) | BR112013028006A2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2835053C (cg-RX-API-DMAC7.html) |
| DK (1) | DK3626737T3 (cg-RX-API-DMAC7.html) |
| ES (1) | ES2970057T3 (cg-RX-API-DMAC7.html) |
| IL (1) | IL229058A (cg-RX-API-DMAC7.html) |
| RU (1) | RU2600886C2 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2012156356A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6591609B2 (en) | 1997-07-15 | 2003-07-15 | New Power Concepts Llc | Regenerator for a Stirling Engine |
| US11384378B2 (en) | 2014-06-04 | 2022-07-12 | Amgen Inc. | Methods for harvesting mammalian cell cultures |
| CN107287265B (zh) * | 2016-03-31 | 2018-09-14 | 正大天晴药业集团南京顺欣制药有限公司 | 一种制备重组人凝血因子ⅷ的方法 |
| WO2020020364A1 (zh) * | 2018-07-26 | 2020-01-30 | 正大天晴药业集团南京顺欣制药有限公司 | 一种制备重组人凝血因子ⅷ的方法 |
| JP2025520392A (ja) * | 2022-06-15 | 2025-07-03 | ユーシービー バイオファルマ エスアールエル | 細胞培養プロセス |
| CN120005720B (zh) * | 2025-04-21 | 2025-08-08 | 深圳市茵冠生物科技有限公司 | 一种人工智能的细胞培养监测方法及系统 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5804420A (en) | 1997-04-18 | 1998-09-08 | Bayer Corporation | Preparation of recombinant Factor VIII in a protein free medium |
| US6358703B1 (en) * | 1998-12-10 | 2002-03-19 | Bayer Corporation | Expression system for factor VIII |
| AU780415B2 (en) * | 1999-07-13 | 2005-03-17 | Biovitrum Ab | Stable Factor VIII compositions |
| EP1266006B1 (en) | 2000-03-22 | 2005-12-07 | Octagene GmbH | Production of recombinant muteins of blood clotting factor viii in human cell lines |
| EP1720972B1 (en) * | 2004-03-05 | 2014-01-08 | DSM IP Assets B.V. | Process for cell culturing by continuous perfusion and alternating tangential flow |
| JP2006296423A (ja) * | 2005-03-24 | 2006-11-02 | Hitachi Ltd | 培養槽の制御装置及び培養装置 |
| EP1707634A1 (en) * | 2005-03-29 | 2006-10-04 | Octapharma AG | Method for isolation of recombinantly produced proteins |
| WO2006103298A2 (en) * | 2005-04-01 | 2006-10-05 | Novo Nordisk Health Care Ag | Blood coagulation fviii analogues |
| EP1739179A1 (en) | 2005-06-30 | 2007-01-03 | Octapharma AG | Serum-free stable transfection and production of recombinant human proteins in human cell lines |
| PL2343362T5 (pl) | 2006-07-14 | 2024-12-02 | Patheon Holdings I B.V. | Ulepszony proces hodowli komórek |
| CN101490239A (zh) * | 2006-07-14 | 2009-07-22 | 帝斯曼知识产权资产管理有限公司 | 改进的细胞培养方法 |
| JPWO2009090787A1 (ja) * | 2008-01-15 | 2011-05-26 | 東レ株式会社 | 生理活性ペプチドまたはタンパク質製造方法、および組換え動物細胞 |
| AU2009264282B2 (en) * | 2008-06-24 | 2013-04-18 | Octapharma Ag | A process of purifying coagulation factor VIII |
| RU2011110459A (ru) | 2008-08-21 | 2012-09-27 | Октафарма АГ (CH) | Рекомбинантно полученный человеческий фактор viii и ix |
| DE102008049120A1 (de) | 2008-09-26 | 2010-04-01 | Bayer Technology Services Gmbh | Verfahren zur Reduzierung von Ablagerungen bei der Kultivierung von Organismen |
| EP2216395A1 (en) | 2009-02-09 | 2010-08-11 | Lonza Biologics plc. | Bioreactor for the cultivation of mammalian cells |
| US8580554B2 (en) * | 2009-07-31 | 2013-11-12 | Baxter International Inc. | Method of producing a polypeptide or virus of interest in a continuous cell culture |
-
2012
- 2012-05-14 DK DK19209635.2T patent/DK3626737T3/da active
- 2012-05-14 EP EP23211876.0A patent/EP4353743A3/en not_active Withdrawn
- 2012-05-14 ES ES19209635T patent/ES2970057T3/es active Active
- 2012-05-14 KR KR1020137029808A patent/KR101980164B1/ko not_active Expired - Fee Related
- 2012-05-14 RU RU2013155382/10A patent/RU2600886C2/ru active
- 2012-05-14 JP JP2014510762A patent/JP6178786B2/ja active Active
- 2012-05-14 CN CN201280021390.XA patent/CN103517919B/zh active Active
- 2012-05-14 AU AU2012257736A patent/AU2012257736B2/en active Active
- 2012-05-14 EP EP19209635.2A patent/EP3626737B1/en active Active
- 2012-05-14 CA CA2835053A patent/CA2835053C/en not_active Expired - Fee Related
- 2012-05-14 EP EP12721821.2A patent/EP2707387B1/en not_active Revoked
- 2012-05-14 BR BR112013028006A patent/BR112013028006A2/pt not_active Application Discontinuation
- 2012-05-14 US US14/113,923 patent/US9115381B2/en active Active
- 2012-05-14 WO PCT/EP2012/058899 patent/WO2012156356A1/en not_active Ceased
-
2013
- 2013-10-24 IL IL229058A patent/IL229058A/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| CN103517919B (zh) | 2016-11-16 |
| KR101980164B1 (ko) | 2019-05-20 |
| JP6178786B2 (ja) | 2017-08-09 |
| JP2014513545A (ja) | 2014-06-05 |
| CA2835053A1 (en) | 2012-11-22 |
| AU2012257736A1 (en) | 2013-10-10 |
| US9115381B2 (en) | 2015-08-25 |
| EP4353743A3 (en) | 2024-07-17 |
| US20140051123A1 (en) | 2014-02-20 |
| BR112013028006A2 (pt) | 2016-09-06 |
| EP2707387B1 (en) | 2019-12-25 |
| IL229058A (en) | 2017-03-30 |
| WO2012156356A1 (en) | 2012-11-22 |
| RU2600886C2 (ru) | 2016-10-27 |
| EP3626737B1 (en) | 2023-11-29 |
| IL229058A0 (en) | 2013-12-31 |
| EP3626737A1 (en) | 2020-03-25 |
| EP4353743A2 (en) | 2024-04-17 |
| AU2012257736B2 (en) | 2015-12-10 |
| EP2707387A1 (en) | 2014-03-19 |
| RU2013155382A (ru) | 2015-06-20 |
| CN103517919A (zh) | 2014-01-15 |
| DK3626737T3 (da) | 2024-02-05 |
| KR20140021009A (ko) | 2014-02-19 |
| ES2970057T3 (es) | 2024-05-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2835053C (en) | A method of increasing the productivity of eucaryotic cells in the production of recombinant fviii | |
| US20240247218A1 (en) | Large scale pei-mediated plasmid transfection | |
| Junne et al. | How scalable and suitable are single-use bioreactors? | |
| Reuveny | Microcarrier culture systems | |
| Birch et al. | Suspension culture of mammalian cells | |
| US8440800B2 (en) | Compositions for reducing cell adhesion to bubbles | |
| EA022219B1 (ru) | Способ получения полипептида или вируса, представляющих интерес, в непрерывной клеточной культуре | |
| US11781102B2 (en) | Large-scale PEI-mediated plasmid transfection | |
| Fenge et al. | Cell culture bioreactors | |
| Kompala et al. | Optimization of high cell density perfusion bioreactors | |
| JP2023503849A (ja) | 接種物を生産するためのプロセスおよびシステム | |
| JP2017511144A (ja) | 高細胞密度フィル・アンド・ドロー発酵プロセス | |
| Kuystermans et al. | Bioreactor systems for producing antibody from mammalian cells | |
| Nilsson et al. | [35] Entrapment of animal cells | |
| ES2777173T3 (es) | Un procedimiento para aumentar la productividad de células eucarióticas en la producción de FVIII recombinante | |
| Ahmed | Application of hydrocyclone for cell separation in mammalian cell perfusion cultures | |
| Hazari et al. | Bioreactor design for vaccine production | |
| Ryan | Growing more cells: A simple guide to small volume cell culture scale-up | |
| Salvador et al. | Bringing cellular agriculture to the table: The role of animal cell bioreactors | |
| Halsall et al. | Systems for cell culture scale-up |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20170321 |
|
| MKLA | Lapsed |
Effective date: 20210514 |