CA2831888C - Pesticides derives d'une toxine contenue dans du venin d'araignee - Google Patents

Pesticides derives d'une toxine contenue dans du venin d'araignee Download PDF

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Publication number
CA2831888C
CA2831888C CA2831888A CA2831888A CA2831888C CA 2831888 C CA2831888 C CA 2831888C CA 2831888 A CA2831888 A CA 2831888A CA 2831888 A CA2831888 A CA 2831888A CA 2831888 C CA2831888 C CA 2831888C
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CA
Canada
Prior art keywords
gna
fusion protein
protein
omega
toxin
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Active
Application number
CA2831888A
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English (en)
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CA2831888A1 (fr
Inventor
John A. Gatehouse
Elaine C. Fitches
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Durham
UK Secretary of State for Environment Food and Rural Affairs
Original Assignee
University of Durham
UK Secretary of State for Environment Food and Rural Affairs
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication of CA2831888A1 publication Critical patent/CA2831888A1/fr
Application granted granted Critical
Publication of CA2831888C publication Critical patent/CA2831888C/fr
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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46—N-acyl derivatives
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/50—1,3-Diazoles; Hydrogenated 1,3-diazoles
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/42—Lectins, e.g. concanavalin, phytohaemagglutinin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Pest Control & Pesticides (AREA)
  • Cell Biology (AREA)
  • Insects & Arthropods (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)

Abstract

La présente invention concerne le développement de protéines de fusion sur la base de toxines peptidiques, en tant que pesticides. L'invention concerne également des plantes transgéniques codant pour lesdits peptides et leur utilité dans la gestion des nuisibles.
CA2831888A 2011-03-31 2012-03-29 Pesticides derives d'une toxine contenue dans du venin d'araignee Active CA2831888C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1105418.6 2011-03-31
GBGB1105418.6A GB201105418D0 (en) 2011-03-31 2011-03-31 Pesticide
PCT/GB2012/000287 WO2012131302A1 (fr) 2011-03-31 2012-03-29 Pesticides

Publications (2)

Publication Number Publication Date
CA2831888A1 CA2831888A1 (fr) 2012-10-04
CA2831888C true CA2831888C (fr) 2019-11-26

Family

ID=44071697

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2831888A Active CA2831888C (fr) 2011-03-31 2012-03-29 Pesticides derives d'une toxine contenue dans du venin d'araignee

Country Status (7)

Country Link
US (1) US10117433B2 (fr)
EP (1) EP2691414B1 (fr)
CA (1) CA2831888C (fr)
ES (1) ES2674324T3 (fr)
GB (1) GB201105418D0 (fr)
PL (1) PL2691414T3 (fr)
WO (1) WO2012131302A1 (fr)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201105418D0 (en) 2011-03-31 2011-05-18 Univ Durham Pesticide
GB201321938D0 (en) * 2013-12-11 2014-01-22 Univ Durham Pesticidal fusion protein improvements
AR099876A1 (es) 2014-03-28 2016-08-24 Monsanto Technology Llc Proteínas de toxinas pesticidas activas contra insectos coleópteros
US10316329B2 (en) 2014-10-16 2019-06-11 Monsanto Technology Llc Proteins toxic or inhibitory to lepidopteran insects
US10487123B2 (en) 2014-10-16 2019-11-26 Monsanto Technology Llc Chimeric insecticidal proteins toxic or inhibitory to lepidopteran pests
PE20170942A1 (es) 2014-10-16 2017-07-13 Monsanto Technology Llc Proteinas de variantes de secuencias de aminoacidos de cry1da1 activas para lepidopteros
NZ768151A (en) 2014-10-16 2023-12-22 Monsanto Technology Llc Novel chimeric insecticidal proteins toxic or inhibitory to lepidopteran pests
BR112017015341A2 (pt) 2015-01-15 2018-01-09 Pioneer Hi Bred Int polipeptídeo inseticida e seu uso, composição inseticida, polinucleotídeo recombinante, construto de dna, planta transgênica ou célula de planta, método para inibir o crescimento, método para controlar a infestação de insetos
CA2996295A1 (fr) 2015-08-27 2017-03-02 Monsanto Technology Llc Nouvelles proteines insecticides
EP3522720A4 (fr) 2016-09-30 2020-04-29 Dow Agrosciences LLC Toxines cry insecticides binaires
EP3592800A4 (fr) * 2017-03-10 2021-01-06 Bolt Threads, Inc. Compositions et procédés de production de rendements sécrétés élevés de protéines recombinées
EP3592762A4 (fr) 2017-03-10 2020-12-30 Bolt Threads, Inc. Compositions et procédés de production de rendements sécrétés élevés de protéines recombinées
CN110475867A (zh) * 2017-04-04 2019-11-19 贝勒大学 靶向型杀蚊毒素
WO2019002155A1 (fr) 2017-06-27 2019-01-03 Lonza Ltd Compositions molluscicides
MX2023007427A (es) 2020-12-21 2023-07-03 Monsanto Technology Llc Proteinas inhibidoras de insectos novedosas.
UY39585A (es) 2020-12-23 2022-07-29 Monsanto Technology Llc Proteínas que exhiben actividad inhibidora de insectos frente a plagas con importancia agrícola de plantas de cultivo y semillas
EP4271188A1 (fr) 2020-12-31 2023-11-08 Monsanto Technology LLC Nouvelles protéines insecticides
WO2023180750A1 (fr) 2022-03-24 2023-09-28 University Of Durham Application topique de protéines de fusion de toxine recombinante insecticide

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763568A (en) 1992-01-31 1998-06-09 Zeneca Limited Insecticidal toxins derived from funnel web (atrax or hadronyche) spiders
ATE207962T1 (de) 1992-04-13 2001-11-15 Syngenta Ltd Dna-konstruktionen und diese enthaltende pflanzen
GB9406371D0 (en) * 1994-03-30 1994-05-25 Axis Genetics Ltd Nematicidal proteins
KR20010043627A (ko) * 1998-05-08 2001-05-25 아메리칸 사이아나미드 캄파니 재조합 바큐로바이러스에 기초한 살충제
US7279547B2 (en) 2000-02-10 2007-10-09 University Of Connecticut Insecticidal compounds and methods for selection thereof
GB0119274D0 (en) * 2001-08-08 2001-10-03 Univ Durham Fusion proteins for insect control
DE602005010295D1 (de) 2004-11-04 2008-11-20 Univ Connecticut Insektizide polypeptide und deren verwendung
US7575758B2 (en) 2005-09-16 2009-08-18 The University Of Connecticut Acaricidal compositions and methods of use thereof
GB201105418D0 (en) 2011-03-31 2011-05-18 Univ Durham Pesticide
NZ621552A (en) 2011-08-24 2016-12-23 Univ Queensland Pest-controlling agents isolated from spider venom and uses thereof
GB201321938D0 (en) 2013-12-11 2014-01-22 Univ Durham Pesticidal fusion protein improvements

Also Published As

Publication number Publication date
PL2691414T3 (pl) 2018-10-31
US20140366227A1 (en) 2014-12-11
ES2674324T3 (es) 2018-06-28
WO2012131302A1 (fr) 2012-10-04
US10117433B2 (en) 2018-11-06
EP2691414A1 (fr) 2014-02-05
CA2831888A1 (fr) 2012-10-04
GB201105418D0 (en) 2011-05-18
EP2691414B1 (fr) 2018-05-02

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Effective date: 20170228