CA2828669C - Method for isolating osteopontin using feeds containing cmp or casein species - Google Patents
Method for isolating osteopontin using feeds containing cmp or casein species Download PDFInfo
- Publication number
- CA2828669C CA2828669C CA2828669A CA2828669A CA2828669C CA 2828669 C CA2828669 C CA 2828669C CA 2828669 A CA2828669 A CA 2828669A CA 2828669 A CA2828669 A CA 2828669A CA 2828669 C CA2828669 C CA 2828669C
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- milk
- derived feed
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- derived
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Links
- 102000004264 Osteopontin Human genes 0.000 title claims abstract description 148
- 108010081689 Osteopontin Proteins 0.000 title claims abstract description 148
- 238000000034 method Methods 0.000 title claims abstract description 85
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 title description 30
- 235000021240 caseins Nutrition 0.000 title description 30
- 239000005018 casein Substances 0.000 title description 25
- 235000013336 milk Nutrition 0.000 claims abstract description 288
- 239000008267 milk Substances 0.000 claims abstract description 288
- 210000004080 milk Anatomy 0.000 claims abstract description 288
- 108010046377 Whey Proteins Proteins 0.000 claims abstract description 74
- 102000007544 Whey Proteins Human genes 0.000 claims abstract description 71
- 239000005862 Whey Substances 0.000 claims abstract description 62
- 108010067454 caseinomacropeptide Proteins 0.000 claims abstract description 43
- 235000009508 confectionery Nutrition 0.000 claims abstract description 34
- 210000002966 serum Anatomy 0.000 claims abstract description 28
- 235000018102 proteins Nutrition 0.000 claims description 147
- 102000004169 proteins and genes Human genes 0.000 claims description 147
- 108090000623 proteins and genes Proteins 0.000 claims description 147
- 238000005349 anion exchange Methods 0.000 claims description 72
- 239000000203 mixture Substances 0.000 claims description 29
- 238000005406 washing Methods 0.000 claims description 13
- 238000005571 anion exchange chromatography Methods 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 9
- 239000002244 precipitate Substances 0.000 claims description 9
- 102000004407 Lactalbumin Human genes 0.000 claims description 8
- 108090000942 Lactalbumin Proteins 0.000 claims description 8
- 235000021241 α-lactalbumin Nutrition 0.000 claims description 8
- 102000008192 Lactoglobulins Human genes 0.000 claims description 6
- 108010060630 Lactoglobulins Proteins 0.000 claims description 6
- 238000001704 evaporation Methods 0.000 claims description 5
- 230000008020 evaporation Effects 0.000 claims description 5
- 238000011026 diafiltration Methods 0.000 claims description 4
- 238000001694 spray drying Methods 0.000 claims description 4
- 150000001768 cations Chemical class 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 2
- 238000006467 substitution reaction Methods 0.000 claims description 2
- 238000005115 demineralization Methods 0.000 claims 1
- 108010076119 Caseins Proteins 0.000 abstract description 84
- 102000011632 Caseins Human genes 0.000 abstract description 84
- 235000021247 β-casein Nutrition 0.000 abstract description 33
- 238000002955 isolation Methods 0.000 abstract description 6
- 239000002253 acid Substances 0.000 description 28
- 235000021249 α-casein Nutrition 0.000 description 20
- 239000012141 concentrate Substances 0.000 description 17
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- 238000002474 experimental method Methods 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- 235000021119 whey protein Nutrition 0.000 description 12
- 239000000243 solution Substances 0.000 description 11
- 230000000694 effects Effects 0.000 description 10
- 238000004519 manufacturing process Methods 0.000 description 8
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- 102000004506 Blood Proteins Human genes 0.000 description 6
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- 239000007790 solid phase Substances 0.000 description 6
- 230000002378 acidificating effect Effects 0.000 description 5
- 239000007864 aqueous solution Substances 0.000 description 5
- 229940021722 caseins Drugs 0.000 description 5
- 125000002091 cationic group Chemical group 0.000 description 5
- 239000003480 eluent Substances 0.000 description 5
- 238000004128 high performance liquid chromatography Methods 0.000 description 5
- 229920001282 polysaccharide Polymers 0.000 description 5
- 108090000765 processed proteins & peptides Proteins 0.000 description 5
- 235000020183 skimmed milk Nutrition 0.000 description 5
- 238000000108 ultra-filtration Methods 0.000 description 5
- -1 0.1 M NaCI Chemical compound 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 235000013351 cheese Nutrition 0.000 description 4
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- 241000894007 species Species 0.000 description 4
- 241000283690 Bos taurus Species 0.000 description 3
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000010923 batch production Methods 0.000 description 3
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- 108010058314 rennet Proteins 0.000 description 3
- 239000012465 retentate Substances 0.000 description 3
- ZOOGRGPOEVQQDX-UUOKFMHZSA-N 3',5'-cyclic GMP Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=C(NC2=O)N)=C2N=C1 ZOOGRGPOEVQQDX-UUOKFMHZSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- 238000007696 Kjeldahl method Methods 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 239000012901 Milli-Q water Substances 0.000 description 2
- 108010001441 Phosphopeptides Proteins 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 235000011148 calcium chloride Nutrition 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
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- 238000005227 gel permeation chromatography Methods 0.000 description 2
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- 230000006651 lactation Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000001471 micro-filtration Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 235000021243 milk fat Nutrition 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 238000010979 pH adjustment Methods 0.000 description 2
- 230000020477 pH reduction Effects 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 125000001453 quaternary ammonium group Chemical group 0.000 description 2
- 235000020185 raw untreated milk Nutrition 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 229940108461 rennet Drugs 0.000 description 2
- 125000000467 secondary amino group Chemical group [H]N([*:1])[*:2] 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 235000021246 κ-casein Nutrition 0.000 description 2
- CHRJZRDFSQHIFI-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;styrene Chemical compound C=CC1=CC=CC=C1.C=CC1=CC=CC=C1C=C CHRJZRDFSQHIFI-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- DFYULHRIYLAUJM-UHFFFAOYSA-N 3,4-diiodobenzoic acid Chemical compound OC(=O)C1=CC=C(I)C(I)=C1 DFYULHRIYLAUJM-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 235000002198 Annona diversifolia Nutrition 0.000 description 1
- 102000005701 Calcium-Binding Proteins Human genes 0.000 description 1
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- 241000282836 Camelus dromedarius Species 0.000 description 1
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- 241000283073 Equus caballus Species 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
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- 241000282842 Lama glama Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- CERQOIWHTDAKMF-UHFFFAOYSA-M Methacrylate Chemical compound CC(=C)C([O-])=O CERQOIWHTDAKMF-UHFFFAOYSA-M 0.000 description 1
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- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 description 1
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- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/20—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from milk, e.g. casein; from whey
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Peptides Or Proteins (AREA)
- Dairy Products (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161448775P | 2011-03-03 | 2011-03-03 | |
| EP11156826.7 | 2011-03-03 | ||
| EP11156826 | 2011-03-03 | ||
| US61/448,775 | 2011-03-03 | ||
| PCT/EP2012/053748 WO2012117119A1 (en) | 2011-03-03 | 2012-03-05 | Method for isolating osteopontin using feeds containing cmp or casein species |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2828669A1 CA2828669A1 (en) | 2012-09-07 |
| CA2828669C true CA2828669C (en) | 2019-06-04 |
Family
ID=44202290
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2828746A Active CA2828746C (en) | 2011-03-03 | 2012-03-05 | Method for isolating osteopontin using concentrated feeds |
| CA2828669A Active CA2828669C (en) | 2011-03-03 | 2012-03-05 | Method for isolating osteopontin using feeds containing cmp or casein species |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2828746A Active CA2828746C (en) | 2011-03-03 | 2012-03-05 | Method for isolating osteopontin using concentrated feeds |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US9181316B2 (enExample) |
| EP (2) | EP2681230B1 (enExample) |
| JP (2) | JP6012053B2 (enExample) |
| KR (2) | KR101940829B1 (enExample) |
| CN (3) | CN105085649B (enExample) |
| AR (2) | AR088719A1 (enExample) |
| AU (2) | AU2012222312B2 (enExample) |
| BR (2) | BR112013022252B1 (enExample) |
| CA (2) | CA2828746C (enExample) |
| DK (2) | DK2681231T3 (enExample) |
| EA (2) | EA023367B1 (enExample) |
| ES (2) | ES2565380T3 (enExample) |
| MX (2) | MX2013010017A (enExample) |
| PL (2) | PL2681230T3 (enExample) |
| WO (2) | WO2012117119A1 (enExample) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103797682B (zh) * | 2011-04-28 | 2017-08-25 | 佐尔循环公司 | 电池管理参数的病毒式分布 |
| WO2013144247A1 (en) | 2012-03-28 | 2013-10-03 | Arla Foods Amba | Nanoparticle aggregates containing osteopontin and calcium- and/or strontium-containing particles |
| AU2014286123B2 (en) * | 2013-07-05 | 2019-02-28 | Arla Foods Amba | Mammalian milk osteopontin for enhancing immune responsiveness |
| US9661868B2 (en) * | 2013-07-16 | 2017-05-30 | Mead Johnson Nutrition Company | Expanded bed adsorption methods for isolation of basic milk proteins including lactoferrin |
| KR200482960Y1 (ko) | 2016-04-07 | 2017-03-21 | 오숙희 | 고주파 얼굴 축소 시술기 |
| JP2019520856A (ja) * | 2016-07-06 | 2019-07-25 | ビルディング ブロック ニュートリショナルズ，エルエルシーＢｕｉｌｄｉｎｇ Ｂｌｏｃｋ Ｎｕｔｒｉｔｉｏｎａｌｓ， Ｌｌｃ | 栄養処方 |
| EP3538053A1 (en) | 2016-11-08 | 2019-09-18 | Dentherapy Ltd | Compositions comprising fluoride and calcium and method for preparing them |
| AU2018386652A1 (en) * | 2017-12-22 | 2020-05-07 | Societe Des Produits Nestle S.A. | Extracts and their use |
| CN109613140B (zh) * | 2019-01-07 | 2021-10-08 | 雅士利国际集团有限公司 | 食品中酪蛋白磷酸肽的检测方法 |
| IL299098A (en) | 2020-06-15 | 2023-02-01 | Building Block Nutritionals Llc | Nutritional formula |
| GB202010987D0 (en) | 2020-07-16 | 2020-09-02 | Dentherapy Ltd | Oral care compositions and methods |
| WO2022223427A1 (en) * | 2021-04-19 | 2022-10-27 | Société des Produits Nestlé S.A. | Use of milk protein fractions as a source of osteopontin |
| CN113567573B (zh) * | 2021-07-09 | 2023-06-02 | 黑龙江飞鹤乳业有限公司 | 骨桥蛋白的分离方法和分析方法 |
| GB202110114D0 (en) | 2021-07-14 | 2021-08-25 | Dentherapy Ltd | Oral care compositions for use in treaatment |
| WO2024056840A1 (en) | 2022-09-16 | 2024-03-21 | Univerza V Ljubljani | Isolation of osteopontin and glycomacropeptide from whey |
| WO2025008636A1 (en) | 2023-07-06 | 2025-01-09 | Dentherapy Ltd | Oral care compositions for use in the treatment of dental health conditions |
| CN117837766A (zh) * | 2023-12-27 | 2024-04-09 | 美维仕(北京)健康管理有限公司 | 营养组合物及其应用 |
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|---|---|---|---|---|
| EP0963163A4 (en) | 1996-10-01 | 2004-09-08 | Univ Massey | Process for isolating glycomacropeptide from dairy products with a phenylalanine impurity of 0.5 %w/w |
| AU748002B2 (en) | 1997-12-31 | 2002-05-30 | Depuy Orthopaedics, Inc. | Osteopontin-based compositions for enhancing bone repair |
| KR20010043323A (ko) | 1998-05-06 | 2001-05-25 | 제넨테크, 인크. | 이온 교환 크로마토그래피에 의한 단백질 정제 방법 |
| US7018665B2 (en) * | 1999-12-08 | 2006-03-28 | Massey University | Process for separation of whey proteins using a novel anion exchanger |
| US7259243B2 (en) | 2000-01-07 | 2007-08-21 | Arla Foods Amba | Process for isolation of osteopontin from milk |
| NZ507335A (en) * | 2000-10-05 | 2004-10-29 | New Zealand Dairy Board | Bone health compositions derived from milk comprising an acidic protein fraction but that does not contain CGMP |
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