CA2825039C - Method for obtaining a lipid-containing composition from microbial biomass - Google Patents

Method for obtaining a lipid-containing composition from microbial biomass Download PDF

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Publication number
CA2825039C
CA2825039C CA2825039A CA2825039A CA2825039C CA 2825039 C CA2825039 C CA 2825039C CA 2825039 A CA2825039 A CA 2825039A CA 2825039 A CA2825039 A CA 2825039A CA 2825039 C CA2825039 C CA 2825039C
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CA
Canada
Prior art keywords
oil
biomass
lipid
yeast
microbial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2825039A
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English (en)
French (fr)
Other versions
CA2825039A1 (en
Inventor
Marios Avgousti
Timothy Allan Bell
Richard E. Bockrath
Oliver Walter Gutsche
Keith W. Hutchenson
Shu-Chien Liang
Robert D. Orlandi
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Priority to CA3026436A priority Critical patent/CA3026436A1/en
Publication of CA2825039A1 publication Critical patent/CA2825039A1/en
Application granted granted Critical
Publication of CA2825039C publication Critical patent/CA2825039C/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting
    • C11B1/104—Production of fats or fatty oils from raw materials by extracting using super critical gases or vapours
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/20—Shaping or working-up of animal feeding-stuffs by moulding, e.g. making cakes or briquettes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/25—Shaping or working-up of animal feeding-stuffs by extrusion
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physiology (AREA)
  • Fats And Perfumes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Edible Oils And Fats (AREA)
CA2825039A 2011-02-11 2012-02-10 Method for obtaining a lipid-containing composition from microbial biomass Expired - Fee Related CA2825039C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA3026436A CA3026436A1 (en) 2011-02-11 2012-02-10 Method for obtaining a lipid-containing composition from microbial biomass

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US201161441849P 2011-02-11 2011-02-11
US201161441854P 2011-02-11 2011-02-11
US201161441842P 2011-02-11 2011-02-11
US201161441836P 2011-02-11 2011-02-11
US61/441,836 2011-02-11
US61/441,842 2011-02-11
US61/441,854 2011-02-11
US61/441,849 2011-02-11
US201161487019P 2011-05-17 2011-05-17
US61/487,019 2011-05-17
PCT/US2012/024662 WO2012109545A2 (en) 2011-02-11 2012-02-10 Method for obtaining a lipid-containing composition from microbial biomass

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA3026436A Division CA3026436A1 (en) 2011-02-11 2012-02-10 Method for obtaining a lipid-containing composition from microbial biomass

Publications (2)

Publication Number Publication Date
CA2825039A1 CA2825039A1 (en) 2012-08-16
CA2825039C true CA2825039C (en) 2020-04-28

Family

ID=46639218

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2825039A Expired - Fee Related CA2825039C (en) 2011-02-11 2012-02-10 Method for obtaining a lipid-containing composition from microbial biomass
CA3026436A Abandoned CA3026436A1 (en) 2011-02-11 2012-02-10 Method for obtaining a lipid-containing composition from microbial biomass

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA3026436A Abandoned CA3026436A1 (en) 2011-02-11 2012-02-10 Method for obtaining a lipid-containing composition from microbial biomass

Country Status (6)

Country Link
US (2) US8999663B2 (enExample)
EP (1) EP2672833A4 (enExample)
JP (1) JP6061866B2 (enExample)
CN (1) CN103841825B (enExample)
CA (2) CA2825039C (enExample)
WO (1) WO2012109545A2 (enExample)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2561050A1 (en) * 2010-04-22 2013-02-27 E.I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
EP2734626B1 (en) * 2011-07-21 2020-12-16 DSM IP Assets B.V. Microbial oils enriched in polyunsaturated fatty acids
CA2894709C (en) 2012-12-24 2022-08-09 Qualitas Health, Ltd. Eicosapentaenoic acid (epa) formulations
ES2872449T3 (es) * 2013-12-04 2021-11-02 Nippon Suisan Kaisha Ltd Aceite microbiano, procedimiento para fabricar aceite microbiano, aceite microbiano concentrado y procedimiento para fabricar aceite microbiano concentrado
CL2013003813A1 (es) * 2013-12-31 2014-06-06 Univ Pontificia Catolica Chile Procedimiento para la obtención de carotenoides (en particular astaxantina) a partir de un sustrato de microalgas en polvo, el que se comprime para formar pellets, los cuales son sometidos a extracción por dioxido de carbono (co2) supercrítico.
CN120623044A (zh) * 2014-09-17 2025-09-12 株式会社日水 含有二十碳五烯酸烷基酯的组合物及其制造方法
CA2958439C (en) 2014-10-02 2022-09-20 Evonik Industries Ag Feedstuff of high abrasion resistance and good stability in water, containing pufas
CN106793803B (zh) * 2014-10-02 2021-03-09 赢创运营有限公司 通过挤压含pufa的生物质来制备含pufa的饲料的方法
BR112017005388B1 (pt) 2014-10-02 2022-09-13 Evonik Operations Gmbh Alimento para animais contendo biomassa de aurantiochytrium
CN107075540A (zh) 2014-10-02 2017-08-18 赢创德固赛有限公司 用于制备具有高细胞稳定性的含pufa生物质的方法
US20180303129A1 (en) * 2014-10-24 2018-10-25 Trustees Of Dartmouth College Aquaculture feed formulation and aquaculture product produced with same
BR112017015709A8 (pt) * 2015-01-26 2018-07-31 Roquette Freres Método para preparar uma farinha de microalgas trituradas ricas em lipídio
US20180187121A1 (en) * 2015-06-15 2018-07-05 White Dog Labs, Inc. Method for producing one or more oil-soluble bioproducts
CN104962589B (zh) * 2015-08-05 2019-02-26 嘉必优生物技术(武汉)股份有限公司 一种富含磷脂型多不饱和脂肪酸的微生物油脂及制备方法
CN104962590B (zh) * 2015-08-05 2018-08-17 嘉必优生物技术(武汉)股份有限公司 一种微生物来源的磷脂型多不饱和脂肪酸油脂及制备方法
CN107778337B (zh) * 2016-08-26 2020-12-08 国投生物科技投资有限公司 超临界二氧化碳萃取螺旋藻中糖脂的方法
CN111787936A (zh) * 2018-03-16 2020-10-16 株式会社理光 生产生物材料的提取物和提取残渣的方法、提取物和提取残渣
EP3938339B1 (en) * 2019-03-14 2026-04-29 DSM IP Assets B.V. Methods of obtaining lipids from a microbial cell composition
FI131688B1 (en) * 2019-06-12 2025-09-17 Aurotec Gmbh Thin-film treatment apparatus
FR3103355B1 (fr) 2019-11-26 2024-05-31 Polaris Composition d’huile de micro-organismes enrichie en acides gras polyinsaturés
CN115968405A (zh) * 2020-02-10 2023-04-14 C16生物科学有限公司 微生物生产的棕榈油替代品
CN111822716A (zh) * 2020-07-21 2020-10-27 泉州华大超硬工具科技有限公司 一种新型工艺串珠的制备方法
JP7653446B2 (ja) * 2020-10-02 2025-03-28 日本たばこ産業株式会社 たばこシート
WO2022104063A2 (en) * 2020-11-12 2022-05-19 C16 Biosciences, Inc. Personal care compositions comprising microbially produced oil
EP4243627A4 (en) * 2020-11-12 2024-10-16 C16 Biosciences, Inc. Edible microbial oil
JP2024538145A (ja) * 2021-10-17 2024-10-18 ライコード・リミテツド フィトエン含有オレオレジン及び組成物
NL2039213B1 (en) * 2024-12-02 2026-06-23 Nopalm Ingredients B V Method for extracting oil from microbial biomass
TWI901501B (zh) * 2025-01-09 2025-10-11 昭輝實業股份有限公司 利用模擬移動床(smb)從藻油萃取並分離高濃度dha及epa的製程方法

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4675132A (en) 1985-03-28 1987-06-23 The United States Of America As Represented By The Secretary Of Commerce Polyunsaturated fatty acids from fish oils
IT1205043B (it) * 1987-05-28 1989-03-10 Innova Di Ridolfi Flora & C S Procedimento per l'estrazione di esteri di acidi grassi poliinsaturi da olii di pesce e composizioni farmaceutiche e dietetiche contenenti detti esteri
GB2218984B (en) 1988-05-27 1992-09-23 Renafield Limited Process for preparing high-concentration mixtures of polyunsaturated fatty acids & their esters and their prophylactic or therapeutic uses
JPH0611793B2 (ja) * 1989-08-17 1994-02-16 旭化成工業株式会社 微粒化セルロース系素材の懸濁液及びその製造方法
EP0442184A1 (en) 1990-02-15 1991-08-21 Campbell Soup Company Production of low cholesterol animal fat by short path distillation
US20050027004A1 (en) * 1993-06-09 2005-02-03 Martek Biosciences Corporation Methods of treating senile dementia and Alzheimer's diseases using docosahexaenoic acid and arachidonic acid compositions
ZA964248B (en) 1995-06-23 1997-11-27 Du Pont Uniform mixtures of pesticidal granules.
CA2579516A1 (en) * 1996-03-28 1997-10-09 Hendrik Louis Bijl Process for the preparation of a granular microbial biomass and isolation of valuable compounds therefrom
US6255505B1 (en) * 1996-03-28 2001-07-03 Gist-Brocades, B.V. Microbial polyunsaturated fatty acid containing oil from pasteurised biomass
JP2000508888A (ja) * 1996-03-28 2000-07-18 ヒスト―ブロカデス・ベスローテン・フェンノートシャップ 低温殺菌したバイオマスからの微生物多不飽和脂肪酸含有オイルの調製
US6166230A (en) 1996-05-15 2000-12-26 Gist-Brocades B.V. Sterol extraction with polar solvent to give low sterol, high triglyceride, microbial oil
US5786188A (en) * 1996-06-05 1998-07-28 The United States Of America As Represented By The Secretary Of Agriculture Fungal inoculum preparation
EP1007228B1 (en) 1997-03-14 2003-09-10 E.I. Du Pont De Nemours And Company Apparatus for breaking and separating particles
TW533235B (en) 1997-06-11 2003-05-21 Idemitsu Petrochemical Co Method for extracting fat-soluble components from microbial cells
SE0001386L (sv) 2000-04-13 2001-10-14 Net Insight Ab Method and apparatus for data transfer between circuit switched and packet switched environments
EP1178118A1 (en) * 2000-08-02 2002-02-06 Dsm N.V. Isolation of microbial oils
EP1453583B1 (en) 2001-12-12 2011-08-31 Martek Biosciences Corporation Extraction and winterization of lipids from biomass
AU2003281542A1 (en) * 2002-06-19 2004-02-09 Dsm Ip Assets B.V. Preparation of microbial oil containing polyunsaturated fatty acids
FI116627B (fi) 2002-11-01 2006-01-13 Danisco Menetelmä triglyseridien rasvahappoketjukoostumuksen säätelemiseksi sekä niiden käyttö
US8313911B2 (en) 2003-05-07 2012-11-20 E I Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7550286B2 (en) 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
CA2610150C (en) 2005-06-23 2015-11-24 Dsm Ip Assets B.V. Process for obtaining lipid from cells
MY139389A (en) 2005-07-20 2009-09-30 Malaysian Palm Oil Board Mpob Production of edible oil with high diglyceride content
US7977498B2 (en) * 2005-08-26 2011-07-12 Ocean Nutrition Canada Limited Reduction of sterols and other compounds from oils
CA2624661C (en) 2005-11-23 2015-06-30 E.I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
US7678560B2 (en) 2006-05-17 2010-03-16 E.I. Du Pont De Nemours And Company Δ 5 desaturase and its use in making polyunsaturated fatty acids
US7695950B2 (en) 2006-05-17 2010-04-13 E. I. Du Pont De Nemours And Company Δ5 desaturase and its use in making polyunsaturated fatty acids
DK2087105T3 (da) 2006-10-30 2015-05-26 Du Pont Delta 17-desaturase og anvendelse heraf ved fremstilling af flerumættede fedtsyrer
BRPI0808577A2 (pt) 2007-04-03 2019-09-24 E.I. Du Pont De Nemours And Company multienzima, polinuleotídeos isolados, construção recombinante, célula hospedeira, yarrowia sp. transformada, método de transformaçao celular, método para a produção de uma planta transformada, de leveduras, planta, sementes, óleos, produtos alimentícios ou rações, bebidas, moléculas isoladas de ácido nucléico, método de produção de uma multienzima, método para alterar o perfil de ácidos graxos, progênie, células hospedeiras microbianas recombinantes, métodos para conversão de ácido linoléico para ácido dihomo gamma-linolênico, ácido a-linolênico para ácido eicosatrienóico, ácido eicosapentaenóico para ácido docosahexaenóico, ácido araquidônico para ácido docosapentaenóico, método para a identificação de um polipeptídeo
US8957280B2 (en) 2007-05-03 2015-02-17 E. I. Du Pont De Nemours And Company Delta-5 desaturases and their use in making polyunsaturated fatty acids
EP2147109B1 (en) * 2007-05-24 2014-03-19 E. I. Du Pont de Nemours and Company Dgat genes from yarrowia lipolytica for increased seed storage lipid production and altered fatty acid profiles in soybean
KR101502191B1 (ko) * 2007-07-30 2015-03-12 닛폰 스이산 가부시키가이샤 Epa 농축유 및 dha 농축유의 제조 방법
US8323935B2 (en) 2007-10-03 2012-12-04 E I Du Pont De Nemours And Company Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production
US20090155447A1 (en) 2007-12-12 2009-06-18 Solae, Llc Protein extrudates comprising omega-3 fatty acids
US20100170144A1 (en) * 2008-04-09 2010-07-08 Solazyme, Inc. Hydroprocessing Microalgal Oils
GB0813598D0 (en) * 2008-07-24 2008-09-03 Pharma Marine As Process
WO2010033753A2 (en) 2008-09-19 2010-03-25 E. I. Du Pont De Nemours And Company Mutant delta5 desaturases and their use in making polyunsaturated fatty acids
CN102271525B (zh) 2008-10-14 2015-01-07 索拉兹米公司 微藻生物质的食品组合物
EP2358862B1 (en) 2008-12-18 2017-08-02 E. I. du Pont de Nemours and Company Reducing byproduction of malonates in a fermentation process
US8637298B2 (en) 2009-06-16 2014-01-28 E I Du Pont De Nemours And Company Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
US9321715B2 (en) 2009-12-30 2016-04-26 Basf Pharma (Callanish) Limited Simulated moving bed chromatographic separation process
EP2561050A1 (en) 2010-04-22 2013-02-27 E.I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
JP2014510166A (ja) * 2011-02-11 2014-04-24 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 短行程蒸留を使用する微生物源からのトリグリセリド油の精製

Also Published As

Publication number Publication date
AU2012214266A1 (en) 2013-07-25
JP6061866B2 (ja) 2017-01-18
EP2672833A4 (en) 2015-06-10
CN103841825B (zh) 2017-03-22
US8999663B2 (en) 2015-04-07
US20150218484A1 (en) 2015-08-06
EP2672833A2 (en) 2013-12-18
US20130046105A1 (en) 2013-02-21
CN103841825A (zh) 2014-06-04
CA3026436A1 (en) 2012-08-16
WO2012109545A2 (en) 2012-08-16
CA2825039A1 (en) 2012-08-16
WO2012109545A3 (en) 2014-02-20
JP2014510165A (ja) 2014-04-24

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