CA2821135A1 - Xylose utilizing zymomonas mobilis with improved ethanol production in biomass hydrolysate medium - Google Patents

Xylose utilizing zymomonas mobilis with improved ethanol production in biomass hydrolysate medium Download PDF

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Publication number
CA2821135A1
CA2821135A1 CA2821135A CA2821135A CA2821135A1 CA 2821135 A1 CA2821135 A1 CA 2821135A1 CA 2821135 A CA2821135 A CA 2821135A CA 2821135 A CA2821135 A CA 2821135A CA 2821135 A1 CA2821135 A1 CA 2821135A1
Authority
CA
Canada
Prior art keywords
seq
zymomonas
ethanol
xylose
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2821135A
Other languages
English (en)
French (fr)
Inventor
Perry G. Caimi
William D. Hitz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of CA2821135A1 publication Critical patent/CA2821135A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2821135A 2010-12-17 2011-12-13 Xylose utilizing zymomonas mobilis with improved ethanol production in biomass hydrolysate medium Abandoned CA2821135A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201061424077P 2010-12-17 2010-12-17
US61/424,077 2010-12-17
PCT/US2011/064580 WO2012082711A1 (en) 2010-12-17 2011-12-13 Xylose utilizing zymomonas mobilis with improved ethanol production in biomass hydrolysate medium

Publications (1)

Publication Number Publication Date
CA2821135A1 true CA2821135A1 (en) 2012-06-21

Family

ID=45444731

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2821135A Abandoned CA2821135A1 (en) 2010-12-17 2011-12-13 Xylose utilizing zymomonas mobilis with improved ethanol production in biomass hydrolysate medium

Country Status (8)

Country Link
US (2) US8476048B2 (https=)
EP (1) EP2652121A1 (https=)
JP (1) JP2013545491A (https=)
CN (1) CN103261400A (https=)
AU (1) AU2011344023A1 (https=)
BR (1) BR112013014912B1 (https=)
CA (1) CA2821135A1 (https=)
WO (1) WO2012082711A1 (https=)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2480660T5 (da) 2009-09-23 2020-11-09 Danisco Us Inc Hidtil ukendte glycosylhydrolaseenzymer og anvendelser heraf
AU2010333801B2 (en) 2009-12-23 2015-12-17 Danisco Us Inc. Methods for improving the efficiency of simultaneous saccharification and fermentation reactions
JP6290626B2 (ja) * 2011-03-17 2018-03-14 ダニスコ・ユーエス・インク 糖化プロセスにおける粘度を低下させる方法
US8980578B2 (en) * 2012-06-11 2015-03-17 Codexis, Inc. Fungal beta-xylosidase variants
US8846357B2 (en) 2012-12-20 2014-09-30 E I Du Pont De Nemours And Company Stabilized chlorine dioxide for contamination control in Zymomonas fermentation
US20140273105A1 (en) 2013-03-12 2014-09-18 E I Du Pont De Nemours And Company Gradient pretreatment of lignocellulosic biomass
WO2015024855A1 (en) 2013-08-19 2015-02-26 Syngulon Sa Controlled growth of microorganisms
US9499451B2 (en) 2013-10-10 2016-11-22 E I Du Pont De Nemours And Company Soil conditioner compositions containing lignocellulosic biomass fermentation process syrup
US9725363B2 (en) 2013-10-10 2017-08-08 E I Du Pont De Nemours And Company Lignocellulosic biomass fermentation process co-product fuel for cement kiln
US20150104842A1 (en) 2013-10-10 2015-04-16 E I Du Pont De Nemours And Company Fuel compositions containing lignocellulosic biomass fermentation process syrup
CN103810391B (zh) * 2014-02-21 2017-05-17 清华大学 运动发酵单胞菌生产乙醇中振荡过程的调控方法
US9371546B2 (en) * 2014-06-24 2016-06-21 E I Du Pont De Nemours And Company Enhancing D-xylose and L-arabinose utilization in Zymomonas cells
WO2016044606A1 (en) 2014-09-17 2016-03-24 Danisco Us Inc Simultaneous saccharification and fermentation process in the presence of benzoate
US11932672B2 (en) 2017-12-19 2024-03-19 Syngulon S.A. Fermentation process

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5231020A (en) 1989-03-30 1993-07-27 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5712133A (en) 1994-04-15 1998-01-27 Midwest Research Institute Pentose fermentation by recombinant zymomonas
US5843760A (en) 1994-04-15 1998-12-01 Midwest Research Institute Single zymomonas mobilis strain for xylose and arabinose fermentation
US5514583A (en) 1994-04-15 1996-05-07 Midwest Research Institute Recombinant zymomonas for pentose fermentation
AU2388295A (en) 1994-04-15 1995-11-10 Midwest Research Institute Recombinant zymomonas for pentose fermentation
DE19856136C2 (de) 1998-12-04 2002-10-24 Pasteur Institut Verfahren und Vorrichtung zur Selektion beschleunigter Proliferation lebender Zellen in Suspension
US7223575B2 (en) 2000-05-01 2007-05-29 Midwest Research Institute Zymomonas pentose-sugar fermenting strains and uses thereof
US6566107B1 (en) 2000-11-01 2003-05-20 Midwest Research Institute Recombinant Zymomonas mobilis with improved xylose utilization
US20040231060A1 (en) 2003-03-07 2004-11-25 Athenix Corporation Methods to enhance the activity of lignocellulose-degrading enzymes
EP1869194A2 (en) 2005-04-12 2007-12-26 E.I.Du pont de nemours and company Treatment of biomass to obtain a target chemical
US7741084B2 (en) 2006-09-28 2010-06-22 E. I. Du Pont De Nemours And Company Ethanol production using xylitol synthesis mutant of xylose-utilizing zymomonas
US7629156B2 (en) 2006-09-28 2009-12-08 E.I. Du Pont De Nemours And Company Ethanol production in fermentation of mixed sugars containing xylose
US7741119B2 (en) 2006-09-28 2010-06-22 E. I. Du Pont De Nemours And Company Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production
ATE554175T1 (de) 2007-10-30 2012-05-15 Du Pont Zymomonas mit erhöhter ethanolproduktion in einem medium mit konzentrierten zuckern und acetat
ES2361385T3 (es) 2007-10-30 2011-06-16 E. I. Du Pont De Nemours And Company Proceso para la producción de etanol en un medio que comprende xilosa que emplea una cepa de zymomonas recombinante con expresión de hima reducida.
JP2011515107A (ja) 2008-03-27 2011-05-19 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 改善されたキシロース資化能を有するザイモモナス（Ｚｙｍｏｍｏｎａｓ）
BRPI0906241B8 (pt) 2008-03-27 2019-02-19 Alliance Sustainable Energy molécula de ácido nucleico, gene quimérico, vetor e método de transformação de uma célula bacteriana
CN102159701A (zh) 2008-04-23 2011-08-17 佐治亚技术研究公司 用于生物质发酵的改良的运动发酵单胞菌菌株
US8247208B2 (en) 2008-12-22 2012-08-21 Alliance For Sustainable Energy Llc Zymomonas with improved xylose utilization in stress conditions
US20110143408A1 (en) 2009-06-18 2011-06-16 E. I. Du Pont De Nemours And Company Zymomonas with improved arabinose utilization
CN101781634B (zh) * 2009-12-04 2011-09-28 天津大学 能利用木糖生产乙醇的重组运动发酵单胞菌及发酵方法
US8906235B2 (en) * 2010-04-28 2014-12-09 E I Du Pont De Nemours And Company Process for liquid/solid separation of lignocellulosic biomass hydrolysate fermentation broth
US8623623B2 (en) 2010-06-29 2014-01-07 E I Du Pont De Nemours And Company Xylose utilization in recombinant Zymomonas

Also Published As

Publication number Publication date
BR112013014912B1 (pt) 2021-06-22
EP2652121A1 (en) 2013-10-23
CN103261400A (zh) 2013-08-21
BR112013014912A2 (pt) 2018-05-15
US8569458B2 (en) 2013-10-29
WO2012082711A1 (en) 2012-06-21
AU2011344023A1 (en) 2013-05-23
JP2013545491A (ja) 2013-12-26
US20130177958A1 (en) 2013-07-11
US20120329114A1 (en) 2012-12-27
US8476048B2 (en) 2013-07-02

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Legal Events

Date Code Title Description
FZDE Discontinued

Effective date: 20161214