CA2785913C - Improvements to nucleic acid elution - Google Patents
Improvements to nucleic acid elution Download PDFInfo
- Publication number
- CA2785913C CA2785913C CA2785913A CA2785913A CA2785913C CA 2785913 C CA2785913 C CA 2785913C CA 2785913 A CA2785913 A CA 2785913A CA 2785913 A CA2785913 A CA 2785913A CA 2785913 C CA2785913 C CA 2785913C
- Authority
- CA
- Canada
- Prior art keywords
- dna
- solid matrix
- inulin
- matrix
- peg
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000010828 elution Methods 0.000 title description 5
- 102000039446 nucleic acids Human genes 0.000 title description 5
- 108020004707 nucleic acids Proteins 0.000 title description 5
- 150000007523 nucleic acids Chemical class 0.000 title description 5
- 239000011159 matrix material Substances 0.000 claims abstract description 61
- 239000007787 solid Substances 0.000 claims abstract description 48
- 229920001202 Inulin Polymers 0.000 claims abstract description 28
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 claims abstract description 28
- 229940029339 inulin Drugs 0.000 claims abstract description 28
- 238000000034 method Methods 0.000 claims description 9
- 239000000872 buffer Substances 0.000 claims description 6
- 229920002678 cellulose Polymers 0.000 claims description 5
- 239000001913 cellulose Substances 0.000 claims description 5
- 150000004676 glycans Chemical class 0.000 abstract description 4
- 229920001282 polysaccharide Polymers 0.000 abstract description 4
- 239000005017 polysaccharide Substances 0.000 abstract description 4
- 102000004169 proteins and genes Human genes 0.000 abstract description 2
- 108090000623 proteins and genes Proteins 0.000 abstract description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 15
- 229920001223 polyethylene glycol Polymers 0.000 description 15
- 238000011084 recovery Methods 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 238000011002 quantification Methods 0.000 description 5
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 2
- 230000003466 anti-cipated effect Effects 0.000 description 2
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
- -1 DNA Chemical class 0.000 description 1
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 102000004167 Ribonuclease P Human genes 0.000 description 1
- 108090000621 Ribonuclease P Proteins 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 235000011089 carbon dioxide Nutrition 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 1
- 230000032050 esterification Effects 0.000 description 1
- 238000005886 esterification reaction Methods 0.000 description 1
- 238000005470 impregnation Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229920002523 polyethylene Glycol 1000 Polymers 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Saccharide Compounds (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicinal Preparation (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US29065209P | 2009-12-29 | 2009-12-29 | |
| US61/290,652 | 2009-12-29 | ||
| PCT/EP2010/070389 WO2011080160A1 (en) | 2009-12-29 | 2010-12-21 | Improvements to nucleic acid elution |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2785913A1 CA2785913A1 (en) | 2011-07-07 |
| CA2785913C true CA2785913C (en) | 2019-05-21 |
Family
ID=43743431
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2785913A Active CA2785913C (en) | 2009-12-29 | 2010-12-21 | Improvements to nucleic acid elution |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US20120289690A1 (enExample) |
| EP (1) | EP2519631B1 (enExample) |
| JP (1) | JP5899118B2 (enExample) |
| CN (1) | CN102725406B (enExample) |
| AU (1) | AU2010338349B2 (enExample) |
| CA (1) | CA2785913C (enExample) |
| WO (1) | WO2011080160A1 (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2785913C (en) * | 2009-12-29 | 2019-05-21 | Ge Healthcare Bio-Sciences Corp. | Improvements to nucleic acid elution |
| US9480966B2 (en) | 2012-04-30 | 2016-11-01 | General Electric Company | Substrates and methods for collection, stabilization and elution of biomolecules |
| US9719082B2 (en) | 2013-10-31 | 2017-08-01 | General Electric Company | Substrates and associated methods for elution of nucleic acids |
| US9534214B2 (en) | 2013-10-31 | 2017-01-03 | General Electric Company | Substrates and associated methods for elution of nucleic acids |
| FI4035762T3 (fi) | 2015-09-09 | 2023-12-04 | Drawbridge Health Inc | Laitteita näytteiden keräämistä, stabilointia ja säilytystä varten |
| US10000742B2 (en) * | 2015-11-19 | 2018-06-19 | General Electric Company | Device and method of collection for RNA viruses |
| CN105462961B (zh) * | 2016-01-14 | 2019-04-19 | 苏州市公安局 | 一种提取核酸的方法及试剂盒 |
| GB2590814B (en) | 2017-01-10 | 2021-11-03 | Drawbridge Health Inc | Devices, systems, and methods for sample collection |
| JP2021145642A (ja) | 2020-03-23 | 2021-09-27 | 株式会社リコー | 担体及び検査方法 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990003959A1 (en) | 1988-10-05 | 1990-04-19 | The Flinders University Of South Australia | Solid medium and method for dna storage |
| US5496562A (en) * | 1988-10-05 | 1996-03-05 | Flinders Technologies Pty Ltd | Solid medium and method for DNA storage |
| GB8903593D0 (en) * | 1989-02-16 | 1989-04-05 | Pafra Ltd | Storage of materials |
| US5124444A (en) * | 1989-07-24 | 1992-06-23 | Microprobe Corporation | Lactam-containing compositions and methods useful for the extraction of nucleic acids |
| US5939259A (en) | 1997-04-09 | 1999-08-17 | Schleicher & Schuell, Inc. | Methods and devices for collecting and storing clinical samples for genetic analysis |
| AU4353500A (en) * | 1999-04-30 | 2000-11-17 | Whatman, Inc. | Substrate including anionic detergent for purifying nucleic acid |
| SE9904475D0 (sv) * | 1999-12-08 | 1999-12-08 | Artursson | Nucleic acid delivery system |
| JP3776334B2 (ja) * | 2001-07-11 | 2006-05-17 | 株式会社富士薬品 | イヌリン含有製剤 |
| EP1436405A4 (en) | 2001-08-20 | 2006-01-04 | Whatman Inc | CLEANING AND OBTAINING DNA FROM HIGH-PARTICLES AND SOLIDS SAMPLES |
| DK1442045T3 (da) * | 2001-11-06 | 2009-02-09 | Cortex Biochem Inc | Isolering og oprensning af nukleinsyrer |
| AU2002366048A1 (en) * | 2001-11-15 | 2003-06-10 | Whatman Inc. | Materials and methods for releasing genetic material |
| US20040033546A1 (en) * | 2002-04-10 | 2004-02-19 | The Trustees Of Columbia University In The City Of New York | Novel microarrays and methods of use thereof |
| KR20070011236A (ko) * | 2003-10-23 | 2007-01-24 | 알자 코포레이션 | 미세돌출부 코팅용의 ｄｎａ 안정화 조성물 |
| US20060153920A1 (en) * | 2004-09-24 | 2006-07-13 | Ketan Amin | Lyophilized pharmaceutical compositions |
| US7727718B2 (en) * | 2005-01-04 | 2010-06-01 | Molecular Research Center, Inc. | Reagents for storage and preparation of samples for DNA analysis |
| CA2785913C (en) * | 2009-12-29 | 2019-05-21 | Ge Healthcare Bio-Sciences Corp. | Improvements to nucleic acid elution |
-
2010
- 2010-12-21 CA CA2785913A patent/CA2785913C/en active Active
- 2010-12-21 EP EP10796044.5A patent/EP2519631B1/en active Active
- 2010-12-21 AU AU2010338349A patent/AU2010338349B2/en active Active
- 2010-12-21 US US13/519,391 patent/US20120289690A1/en not_active Abandoned
- 2010-12-21 CN CN201080059859.XA patent/CN102725406B/zh active Active
- 2010-12-21 JP JP2012546416A patent/JP5899118B2/ja active Active
- 2010-12-21 WO PCT/EP2010/070389 patent/WO2011080160A1/en not_active Ceased
-
2020
- 2020-12-15 US US17/122,606 patent/US11725201B2/en active Active
-
2023
- 2023-06-23 US US18/340,609 patent/US12247199B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP5899118B2 (ja) | 2016-04-06 |
| WO2011080160A1 (en) | 2011-07-07 |
| AU2010338349A1 (en) | 2012-06-21 |
| CA2785913A1 (en) | 2011-07-07 |
| CN102725406A (zh) | 2012-10-10 |
| US11725201B2 (en) | 2023-08-15 |
| EP2519631A1 (en) | 2012-11-07 |
| JP2013515494A (ja) | 2013-05-09 |
| EP2519631B1 (en) | 2017-02-15 |
| US20210102190A1 (en) | 2021-04-08 |
| AU2010338349B2 (en) | 2015-03-05 |
| US20230332133A1 (en) | 2023-10-19 |
| US12247199B2 (en) | 2025-03-11 |
| CN102725406B (zh) | 2016-06-01 |
| US20120289690A1 (en) | 2012-11-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20151110 |