CA2779255A1 - Rapid detection of mycoplasma contamination in cell culture samples - Google Patents
Rapid detection of mycoplasma contamination in cell culture samples Download PDFInfo
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- CA2779255A1 CA2779255A1 CA2779255A CA2779255A CA2779255A1 CA 2779255 A1 CA2779255 A1 CA 2779255A1 CA 2779255 A CA2779255 A CA 2779255A CA 2779255 A CA2779255 A CA 2779255A CA 2779255 A1 CA2779255 A1 CA 2779255A1
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- Prior art keywords
- mycoplasma
- seq
- amplification
- primer
- amplification reaction
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Classifications
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
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| US25621609P | 2009-10-29 | 2009-10-29 | |
| US61/256,216 | 2009-10-29 | ||
| PCT/US2010/054658 WO2011059802A1 (en) | 2009-10-29 | 2010-10-29 | Rapid detection of mycoplasma contamination in cell culture samples |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2779255A1 true CA2779255A1 (en) | 2011-05-19 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2779255A Abandoned CA2779255A1 (en) | 2009-10-29 | 2010-10-29 | Rapid detection of mycoplasma contamination in cell culture samples |
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| US (1) | US20110104686A1 (OSRAM) |
| EP (1) | EP2494079A4 (OSRAM) |
| JP (1) | JP2013515458A (OSRAM) |
| CA (1) | CA2779255A1 (OSRAM) |
| WO (1) | WO2011059802A1 (OSRAM) |
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| US9921154B2 (en) | 2011-03-18 | 2018-03-20 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays |
| US9970052B2 (en) | 2012-08-23 | 2018-05-15 | Bio-Rad Laboratories, Inc. | Digital assays with a generic reporter |
| CN104995315A (zh) * | 2012-12-18 | 2015-10-21 | 梅里亚股份有限公司 | Ibv、csfv和ndv的高分辨熔解基因分型 |
| WO2014121239A2 (en) * | 2013-02-01 | 2014-08-07 | Bio-Rad Laboratories, Inc. | Multiplexed digital assay with data exclusion for calculation of target levels |
| US10640834B2 (en) | 2014-09-10 | 2020-05-05 | National University Corporation Tokyo Medical And Dental University | Method for detecting mycoplasma |
| WO2017086394A1 (ja) * | 2015-11-18 | 2017-05-26 | 国立研究開発法人海洋研究開発機構 | 標的核酸の定量方法及びそのためのキット |
| EP3697927B1 (en) | 2017-10-19 | 2022-12-14 | Bio-Rad Laboratories, Inc. | Digital amplification assays with unconventional and/or inverse changes in photoluminescence |
| WO2021222856A1 (en) * | 2020-05-01 | 2021-11-04 | Helix Nanotechnologies, Inc. | Compositions and methods for rna synthesis |
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| EP2322668A3 (en) * | 1999-09-28 | 2011-10-05 | Geneohm Sciences Canada Inc. | Highly conserved gene and its use to generate species-specific, genus-specific, family-specific, group-specific and universal nucleic acid probes for microorganisms. |
| US20040121311A1 (en) * | 2002-12-06 | 2004-06-24 | Ecker David J. | Methods for rapid detection and identification of bioagents in livestock |
| US7560260B2 (en) * | 2002-07-25 | 2009-07-14 | Bio-Rad Laboratories, Inc. | Compositions with polymerase activity |
| US7666645B2 (en) * | 2002-10-23 | 2010-02-23 | Bio-Rad Laboratories, Inc. | Sso7-polymerase conjugate proteins |
| FI115637B (fi) * | 2003-12-19 | 2005-06-15 | Mobidiag Oy | Diagnostinen menetelmä hengitystieinfektioita aiheuttavien bakteerien osoittamiseksi ja tunnistamiseksi ja menetelmässä käyttökelpoinen alukeseos |
| CA2621146A1 (en) * | 2005-09-06 | 2007-03-15 | Gen-Probe Incorporated | Methods, compositions and kits for isothermal amplification of nucleic acids |
| US7872116B2 (en) * | 2007-02-06 | 2011-01-18 | American Type Culture Collection (Atcc) | Identification of cell culture contaminants among Mollicutes species by a PCR based assay |
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2010
- 2010-10-29 US US12/915,485 patent/US20110104686A1/en not_active Abandoned
- 2010-10-29 WO PCT/US2010/054658 patent/WO2011059802A1/en not_active Ceased
- 2010-10-29 JP JP2012537100A patent/JP2013515458A/ja not_active Withdrawn
- 2010-10-29 CA CA2779255A patent/CA2779255A1/en not_active Abandoned
- 2010-10-29 EP EP10830491.6A patent/EP2494079A4/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| EP2494079A1 (en) | 2012-09-05 |
| WO2011059802A1 (en) | 2011-05-19 |
| EP2494079A4 (en) | 2013-06-26 |
| US20110104686A1 (en) | 2011-05-05 |
| JP2013515458A (ja) | 2013-05-09 |
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