CA2766312C - Methods and systems for phylogenetic analysis - Google Patents
Methods and systems for phylogenetic analysis Download PDFInfo
- Publication number
- CA2766312C CA2766312C CA2766312A CA2766312A CA2766312C CA 2766312 C CA2766312 C CA 2766312C CA 2766312 A CA2766312 A CA 2766312A CA 2766312 A CA2766312 A CA 2766312A CA 2766312 C CA2766312 C CA 2766312C
- Authority
- CA
- Canada
- Prior art keywords
- probes
- probe
- bacteria
- sample
- otus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims abstract description 238
- 238000013081 phylogenetic analysis Methods 0.000 title description 5
- 239000000523 sample Substances 0.000 claims abstract description 952
- 238000009396 hybridization Methods 0.000 claims abstract description 155
- 244000005700 microbiome Species 0.000 claims description 185
- 108090000623 proteins and genes Proteins 0.000 claims description 126
- 238000004458 analytical method Methods 0.000 claims description 106
- 150000007523 nucleic acids Chemical group 0.000 claims description 105
- 102000040430 polynucleotide Human genes 0.000 claims description 89
- 108091033319 polynucleotide Proteins 0.000 claims description 89
- 239000002157 polynucleotide Substances 0.000 claims description 89
- 102000039446 nucleic acids Human genes 0.000 claims description 74
- 108020004707 nucleic acids Proteins 0.000 claims description 74
- 238000003556 assay Methods 0.000 claims description 58
- 108020004465 16S ribosomal RNA Proteins 0.000 claims description 54
- 239000002773 nucleotide Substances 0.000 claims description 51
- 125000003729 nucleotide group Chemical group 0.000 claims description 51
- 239000013642 negative control Substances 0.000 claims description 48
- 230000000295 complement effect Effects 0.000 claims description 37
- 239000013641 positive control Substances 0.000 claims description 33
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 22
- 108700022487 rRNA Genes Proteins 0.000 claims description 21
- 201000010099 disease Diseases 0.000 claims description 20
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 19
- 230000008859 change Effects 0.000 claims description 16
- 210000003608 fece Anatomy 0.000 claims description 14
- 230000001965 increasing effect Effects 0.000 claims description 14
- 108020004463 18S ribosomal RNA Proteins 0.000 claims description 10
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 10
- 210000001035 gastrointestinal tract Anatomy 0.000 claims description 10
- 239000013068 control sample Substances 0.000 claims description 8
- 101150055609 fusA gene Proteins 0.000 claims description 8
- 101150013736 gyrB gene Proteins 0.000 claims description 8
- 101150079601 recA gene Proteins 0.000 claims description 8
- 101150090202 rpoB gene Proteins 0.000 claims description 8
- 108020004565 5.8S Ribosomal RNA Proteins 0.000 claims description 7
- 238000005067 remediation Methods 0.000 claims description 6
- 210000000214 mouth Anatomy 0.000 claims description 5
- 239000013074 reference sample Substances 0.000 claims description 5
- 108020005075 5S Ribosomal RNA Proteins 0.000 claims description 4
- 208000011231 Crohn disease Diseases 0.000 claims description 4
- 210000004369 blood Anatomy 0.000 claims description 4
- 239000008280 blood Substances 0.000 claims description 4
- 244000005709 gut microbiome Species 0.000 claims description 4
- 208000002551 irritable bowel syndrome Diseases 0.000 claims description 4
- 210000002345 respiratory system Anatomy 0.000 claims description 4
- 210000003491 skin Anatomy 0.000 claims description 4
- 210000002700 urine Anatomy 0.000 claims description 4
- 206010003645 Atopy Diseases 0.000 claims description 3
- 208000006545 Chronic Obstructive Pulmonary Disease Diseases 0.000 claims description 3
- 206010051606 Necrotising colitis Diseases 0.000 claims description 3
- 206010028980 Neoplasm Diseases 0.000 claims description 3
- 208000008589 Obesity Diseases 0.000 claims description 3
- 208000007107 Stomach Ulcer Diseases 0.000 claims description 3
- 208000006673 asthma Diseases 0.000 claims description 3
- 201000011510 cancer Diseases 0.000 claims description 3
- 206010009887 colitis Diseases 0.000 claims description 3
- 208000004995 necrotizing enterocolitis Diseases 0.000 claims description 3
- 235000020824 obesity Nutrition 0.000 claims description 3
- 201000006195 perinatal necrotizing enterocolitis Diseases 0.000 claims description 3
- 208000028169 periodontal disease Diseases 0.000 claims description 3
- 206010039083 rhinitis Diseases 0.000 claims description 3
- 208000002874 Acne Vulgaris Diseases 0.000 claims description 2
- 201000003883 Cystic fibrosis Diseases 0.000 claims description 2
- 208000004262 Food Hypersensitivity Diseases 0.000 claims description 2
- 206010016946 Food allergy Diseases 0.000 claims description 2
- 206010036790 Productive cough Diseases 0.000 claims description 2
- 206010000496 acne Diseases 0.000 claims description 2
- 210000000481 breast Anatomy 0.000 claims description 2
- 208000035475 disorder Diseases 0.000 claims description 2
- 235000020932 food allergy Nutrition 0.000 claims description 2
- 208000021302 gastroesophageal reflux disease Diseases 0.000 claims description 2
- 210000003802 sputum Anatomy 0.000 claims description 2
- 208000024794 sputum Diseases 0.000 claims description 2
- 108020005187 Oligonucleotide Probes Proteins 0.000 abstract description 94
- 239000002751 oligonucleotide probe Substances 0.000 abstract description 94
- 238000009739 binding Methods 0.000 abstract description 9
- 230000027455 binding Effects 0.000 abstract description 8
- 241000894006 Bacteria Species 0.000 description 667
- 241000192142 Proteobacteria Species 0.000 description 263
- 241000192125 Firmicutes Species 0.000 description 226
- 241001135756 Alphaproteobacteria Species 0.000 description 159
- 241000192700 Cyanobacteria Species 0.000 description 144
- 241000605059 Bacteroidetes Species 0.000 description 131
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 129
- 241001112695 Clostridiales Species 0.000 description 126
- 241000192128 Gammaproteobacteria Species 0.000 description 123
- 241001185307 Rhodobacterales Species 0.000 description 102
- 230000002550 fecal effect Effects 0.000 description 88
- 241001430267 Rhodobacteraceae Species 0.000 description 87
- 241000304886 Bacilli Species 0.000 description 81
- 241001156739 Actinobacteria <phylum> Species 0.000 description 72
- 238000011109 contamination Methods 0.000 description 60
- 238000001514 detection method Methods 0.000 description 55
- 239000000203 mixture Substances 0.000 description 53
- 241001112693 Lachnospiraceae Species 0.000 description 52
- 210000003763 chloroplast Anatomy 0.000 description 50
- 241000305071 Enterobacterales Species 0.000 description 47
- 241000692822 Bacteroidales Species 0.000 description 46
- 241001112696 Clostridia Species 0.000 description 44
- 238000003491 array Methods 0.000 description 42
- 230000000813 microbial effect Effects 0.000 description 41
- 241000193833 Bacillales Species 0.000 description 38
- 241000894007 species Species 0.000 description 38
- 241001112724 Lactobacillales Species 0.000 description 37
- 239000011859 microparticle Substances 0.000 description 37
- 230000004044 response Effects 0.000 description 37
- 238000012360 testing method Methods 0.000 description 36
- 108020004414 DNA Proteins 0.000 description 35
- 230000001580 bacterial effect Effects 0.000 description 34
- 241001180364 Spirochaetes Species 0.000 description 33
- 230000007613 environmental effect Effects 0.000 description 33
- 241001141128 Flavobacteriales Species 0.000 description 32
- 241000947840 Alteromonadales Species 0.000 description 30
- 241001430149 Clostridiaceae Species 0.000 description 30
- 238000009826 distribution Methods 0.000 description 29
- 238000002474 experimental method Methods 0.000 description 29
- 239000010865 sewage Substances 0.000 description 29
- 241000203809 Actinomycetales Species 0.000 description 28
- 241001248480 Alteromonadaceae Species 0.000 description 28
- 108091034117 Oligonucleotide Proteins 0.000 description 28
- 238000012545 processing Methods 0.000 description 27
- 230000000875 corresponding effect Effects 0.000 description 26
- 241000244332 Flavobacteriaceae Species 0.000 description 25
- 239000011324 bead Substances 0.000 description 24
- 238000003752 polymerase chain reaction Methods 0.000 description 24
- 230000008569 process Effects 0.000 description 24
- 241001135755 Betaproteobacteria Species 0.000 description 23
- 108091093088 Amplicon Proteins 0.000 description 22
- 241001135761 Deltaproteobacteria Species 0.000 description 22
- 241000192522 Nostocales Species 0.000 description 22
- 241001141544 Sphingobacteriales Species 0.000 description 22
- 239000012634 fragment Substances 0.000 description 22
- 241001112741 Bacillaceae Species 0.000 description 19
- 210000004027 cell Anatomy 0.000 description 19
- 239000003673 groundwater Substances 0.000 description 19
- 231100000331 toxic Toxicity 0.000 description 19
- 230000002588 toxic effect Effects 0.000 description 19
- 241000230565 Sphingobacteriia Species 0.000 description 18
- 235000015107 ale Nutrition 0.000 description 18
- 239000002689 soil Substances 0.000 description 18
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 18
- 108091036078 conserved sequence Proteins 0.000 description 17
- 235000013305 food Nutrition 0.000 description 17
- 238000005192 partition Methods 0.000 description 17
- 239000013049 sediment Substances 0.000 description 17
- 241001112692 Peptostreptococcaceae Species 0.000 description 16
- 241000589970 Spirochaetales Species 0.000 description 16
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 16
- 238000013459 approach Methods 0.000 description 16
- 230000014509 gene expression Effects 0.000 description 16
- 238000011002 quantification Methods 0.000 description 16
- 241000196324 Embryophyta Species 0.000 description 15
- 241000230562 Flavobacteriia Species 0.000 description 15
- 241001430197 Mollicutes Species 0.000 description 15
- 241000947899 Oceanospirillales Species 0.000 description 15
- 241000589971 Spirochaetaceae Species 0.000 description 15
- 238000012544 monitoring process Methods 0.000 description 15
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 14
- 241001261005 Verrucomicrobia Species 0.000 description 14
- 238000006243 chemical reaction Methods 0.000 description 14
- 239000011521 glass Substances 0.000 description 14
- 241000194018 Streptococcaceae Species 0.000 description 13
- 241001183192 Verrucomicrobiae Species 0.000 description 13
- 230000000694 effects Effects 0.000 description 13
- 239000003921 oil Substances 0.000 description 13
- 230000002829 reductive effect Effects 0.000 description 13
- 241000606126 Bacteroidaceae Species 0.000 description 12
- 241001609975 Enterococcaceae Species 0.000 description 12
- 241000233866 Fungi Species 0.000 description 12
- 241001465754 Metazoa Species 0.000 description 12
- 241000692844 Prevotellaceae Species 0.000 description 12
- 230000003321 amplification Effects 0.000 description 12
- -1 carbocyclic sugars Chemical class 0.000 description 12
- 239000000470 constituent Substances 0.000 description 12
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 12
- 238000009792 diffusion process Methods 0.000 description 12
- 238000004519 manufacturing process Methods 0.000 description 12
- 238000003199 nucleic acid amplification method Methods 0.000 description 12
- 102000004169 proteins and genes Human genes 0.000 description 12
- 238000012163 sequencing technique Methods 0.000 description 12
- 238000003860 storage Methods 0.000 description 12
- 230000001052 transient effect Effects 0.000 description 12
- 241000580482 Acidobacteria Species 0.000 description 11
- 241001142109 Chloroflexi Species 0.000 description 11
- 101100316117 Rattus norvegicus Unc50 gene Proteins 0.000 description 11
- 241000295644 Staphylococcaceae Species 0.000 description 11
- 239000000463 material Substances 0.000 description 11
- 238000002493 microarray Methods 0.000 description 11
- 238000011282 treatment Methods 0.000 description 11
- 241000588921 Enterobacteriaceae Species 0.000 description 10
- 241001468155 Lactobacillaceae Species 0.000 description 10
- 241000589157 Rhizobiales Species 0.000 description 10
- 229910052770 Uranium Inorganic materials 0.000 description 10
- 241000230320 Verrucomicrobiales Species 0.000 description 10
- 238000004422 calculation algorithm Methods 0.000 description 10
- 239000000356 contaminant Substances 0.000 description 10
- 238000013467 fragmentation Methods 0.000 description 10
- 238000006062 fragmentation reaction Methods 0.000 description 10
- 238000005070 sampling Methods 0.000 description 10
- 239000003643 water by type Substances 0.000 description 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 9
- 241000031711 Cytophagaceae Species 0.000 description 9
- 241001265526 Gemmatimonadetes <phylum> Species 0.000 description 9
- 239000003153 chemical reaction reagent Substances 0.000 description 9
- 238000000684 flow cytometry Methods 0.000 description 9
- 238000002372 labelling Methods 0.000 description 9
- 229940113082 thymine Drugs 0.000 description 9
- 241001600148 Burkholderiales Species 0.000 description 8
- 241001571070 Desulfobacteraceae Species 0.000 description 8
- 241001517310 Eria Species 0.000 description 8
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 8
- 241000692845 Rikenellaceae Species 0.000 description 8
- 239000002775 capsule Substances 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 8
- 230000012010 growth Effects 0.000 description 8
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 8
- 229930195733 hydrocarbon Natural products 0.000 description 8
- 150000002430 hydrocarbons Chemical class 0.000 description 8
- 239000011325 microbead Substances 0.000 description 8
- 238000010606 normalization Methods 0.000 description 8
- JFALSRSLKYAFGM-UHFFFAOYSA-N uranium(0) Chemical compound [U] JFALSRSLKYAFGM-UHFFFAOYSA-N 0.000 description 8
- 229930024421 Adenine Natural products 0.000 description 7
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 7
- 241001600130 Comamonadaceae Species 0.000 description 7
- 241000588724 Escherichia coli Species 0.000 description 7
- 241000192121 Nitrospira <genus> Species 0.000 description 7
- 229960000643 adenine Drugs 0.000 description 7
- 238000000605 extraction Methods 0.000 description 7
- 238000007726 management method Methods 0.000 description 7
- 230000018612 quorum sensing Effects 0.000 description 7
- 230000009467 reduction Effects 0.000 description 7
- 230000035945 sensitivity Effects 0.000 description 7
- 239000007787 solid Substances 0.000 description 7
- 229940035893 uracil Drugs 0.000 description 7
- 239000002699 waste material Substances 0.000 description 7
- 241001114462 Anaeroplasmatales Species 0.000 description 6
- 241000203069 Archaea Species 0.000 description 6
- 241000609971 Erysipelotrichaceae Species 0.000 description 6
- 241001135694 Halomonadaceae Species 0.000 description 6
- 108700036248 MT-RNR1 Proteins 0.000 description 6
- 241001660099 Sphingobacteriaceae Species 0.000 description 6
- 241000607479 Yersinia pestis Species 0.000 description 6
- 230000008901 benefit Effects 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 6
- 229940104302 cytosine Drugs 0.000 description 6
- 238000013461 design Methods 0.000 description 6
- 238000001914 filtration Methods 0.000 description 6
- 239000007789 gas Substances 0.000 description 6
- 230000002503 metabolic effect Effects 0.000 description 6
- 102000042567 non-coding RNA Human genes 0.000 description 6
- 235000015097 nutrients Nutrition 0.000 description 6
- 239000003305 oil spill Substances 0.000 description 6
- 230000003287 optical effect Effects 0.000 description 6
- 244000052769 pathogen Species 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 238000003786 synthesis reaction Methods 0.000 description 6
- 239000003053 toxin Substances 0.000 description 6
- 231100000765 toxin Toxicity 0.000 description 6
- 108700012359 toxins Proteins 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 241001453184 Aquificales Species 0.000 description 5
- 241000283690 Bos taurus Species 0.000 description 5
- 241001570499 Campylobacterales Species 0.000 description 5
- 241000031723 Crenotrichaceae Species 0.000 description 5
- 241001571071 Desulfobacterales Species 0.000 description 5
- 241001148568 Epsilonproteobacteria Species 0.000 description 5
- 241001568673 Geobacteraceae Species 0.000 description 5
- 241000282412 Homo Species 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 5
- 241001212101 Nitrosomonadaceae Species 0.000 description 5
- 241001453382 Nitrosomonadales Species 0.000 description 5
- 238000012408 PCR amplification Methods 0.000 description 5
- 241000606752 Pasteurellaceae Species 0.000 description 5
- 241000947860 Pasteurellales Species 0.000 description 5
- 241000863008 Polyangiaceae Species 0.000 description 5
- 241000235070 Saccharomyces Species 0.000 description 5
- 230000003115 biocidal effect Effects 0.000 description 5
- 238000010276 construction Methods 0.000 description 5
- 238000007405 data analysis Methods 0.000 description 5
- 230000007423 decrease Effects 0.000 description 5
- 230000001419 dependent effect Effects 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 239000013505 freshwater Substances 0.000 description 5
- 230000002538 fungal effect Effects 0.000 description 5
- 238000003500 gene array Methods 0.000 description 5
- 230000036541 health Effects 0.000 description 5
- 238000003384 imaging method Methods 0.000 description 5
- 238000003780 insertion Methods 0.000 description 5
- 230000037431 insertion Effects 0.000 description 5
- 239000011159 matrix material Substances 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 238000012372 quality testing Methods 0.000 description 5
- 238000003753 real-time PCR Methods 0.000 description 5
- 238000011084 recovery Methods 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 238000010186 staining Methods 0.000 description 5
- 238000007619 statistical method Methods 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- 230000001018 virulence Effects 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- 241000203026 Acholeplasmataceae Species 0.000 description 4
- 241001114404 Acholeplasmatales Species 0.000 description 4
- 241001662476 Acidimicrobiales Species 0.000 description 4
- 241001185328 Acidobacteriaceae Species 0.000 description 4
- 241001185327 Acidobacteriales Species 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 241000271566 Aves Species 0.000 description 4
- 241000404109 Catabacter Species 0.000 description 4
- 241000191368 Chlorobi Species 0.000 description 4
- 241000872416 Dehalococcoidia Species 0.000 description 4
- 241001571085 Desulfovibrionales Species 0.000 description 4
- 241000970811 Dictyoglomi Species 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 241001608234 Faecalibacterium Species 0.000 description 4
- 241001135750 Geobacter Species 0.000 description 4
- 241000204888 Geobacter sp. Species 0.000 description 4
- 206010020751 Hypersensitivity Diseases 0.000 description 4
- 241001648288 Kineosporiaceae Species 0.000 description 4
- 241001655310 Microbacteriaceae Species 0.000 description 4
- 241000863434 Myxococcales Species 0.000 description 4
- 241001141101 Nitrospirales Species 0.000 description 4
- 241000589949 Planctomycetales Species 0.000 description 4
- 241001180192 Planctomycetia Species 0.000 description 4
- 208000004891 Shellfish Poisoning Diseases 0.000 description 4
- 241000107801 Shewanellaceae Species 0.000 description 4
- 241000131972 Sphingomonadaceae Species 0.000 description 4
- 241001185305 Sphingomonadales Species 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 4
- 241001248478 Thiotrichales Species 0.000 description 4
- 241000607626 Vibrio cholerae Species 0.000 description 4
- 230000002159 abnormal effect Effects 0.000 description 4
- 230000007815 allergy Effects 0.000 description 4
- 238000009360 aquaculture Methods 0.000 description 4
- 244000144974 aquaculture Species 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 238000004364 calculation method Methods 0.000 description 4
- 230000002596 correlated effect Effects 0.000 description 4
- 230000034994 death Effects 0.000 description 4
- 239000003651 drinking water Substances 0.000 description 4
- 230000006870 function Effects 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 239000004005 microsphere Substances 0.000 description 4
- 230000006855 networking Effects 0.000 description 4
- 108091027963 non-coding RNA Proteins 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 238000000206 photolithography Methods 0.000 description 4
- 230000000717 retained effect Effects 0.000 description 4
- 210000003705 ribosome Anatomy 0.000 description 4
- 235000015170 shellfish Nutrition 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000002560 therapeutic procedure Methods 0.000 description 4
- 239000000304 virulence factor Substances 0.000 description 4
- 241000251468 Actinopterygii Species 0.000 description 3
- 241001136700 Anaerolineae Species 0.000 description 3
- 241000606125 Bacteroides Species 0.000 description 3
- 241000253402 Burkholderiaceae Species 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 239000004215 Carbon black (E152) Substances 0.000 description 3
- 244000132059 Carica parviflora Species 0.000 description 3
- 235000014653 Carica parviflora Nutrition 0.000 description 3
- 241000947907 Chromatiales Species 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
- 238000007400 DNA extraction Methods 0.000 description 3
- 238000013382 DNA quantification Methods 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 241000194033 Enterococcus Species 0.000 description 3
- 241001112690 Eubacteriaceae Species 0.000 description 3
- 241000206602 Eukaryota Species 0.000 description 3
- VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 description 3
- 241001453172 Fusobacteria Species 0.000 description 3
- 101000852870 Homo sapiens Interferon alpha/beta receptor 1 Proteins 0.000 description 3
- 241000278301 Hydrogenothermaceae Species 0.000 description 3
- 102100036714 Interferon alpha/beta receptor 1 Human genes 0.000 description 3
- 241000936934 Kiritimatiellaeota Species 0.000 description 3
- 241000192017 Micrococcaceae Species 0.000 description 3
- 241001141103 Nitrospiraceae Species 0.000 description 3
- 229910019142 PO4 Inorganic materials 0.000 description 3
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 3
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 3
- 241001180199 Planctomycetes Species 0.000 description 3
- 241000192665 Plectonema Species 0.000 description 3
- 239000004793 Polystyrene Substances 0.000 description 3
- 241000692843 Porphyromonadaceae Species 0.000 description 3
- 241000192511 Pseudanabaena Species 0.000 description 3
- 241001248479 Pseudomonadales Species 0.000 description 3
- 241001277912 Rhodocyclaceae Species 0.000 description 3
- 241001212087 Rhodocyclales Species 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 241001141092 Thermomicrobia Species 0.000 description 3
- 241000947895 Thiotrichaceae Species 0.000 description 3
- 241000934136 Verruca Species 0.000 description 3
- 241001183271 Verrucomicrobiaceae Species 0.000 description 3
- 208000000260 Warts Diseases 0.000 description 3
- 238000007792 addition Methods 0.000 description 3
- 208000026935 allergic disease Diseases 0.000 description 3
- 230000000692 anti-sense effect Effects 0.000 description 3
- 229960002685 biotin Drugs 0.000 description 3
- 239000011616 biotin Substances 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 238000012512 characterization method Methods 0.000 description 3
- 210000000349 chromosome Anatomy 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- 238000007621 cluster analysis Methods 0.000 description 3
- 239000002131 composite material Substances 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 235000005911 diet Nutrition 0.000 description 3
- 235000020188 drinking water Nutrition 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 238000012165 high-throughput sequencing Methods 0.000 description 3
- 230000001976 improved effect Effects 0.000 description 3
- 238000011065 in-situ storage Methods 0.000 description 3
- 238000010348 incorporation Methods 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 238000009434 installation Methods 0.000 description 3
- 239000004816 latex Substances 0.000 description 3
- 229920000126 latex Polymers 0.000 description 3
- 238000002844 melting Methods 0.000 description 3
- 230000008018 melting Effects 0.000 description 3
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 238000002887 multiple sequence alignment Methods 0.000 description 3
- 101150101270 nifD gene Proteins 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 239000012071 phase Substances 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
- 239000010452 phosphate Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 229920002223 polystyrene Polymers 0.000 description 3
- 230000002441 reversible effect Effects 0.000 description 3
- 239000010703 silicon Substances 0.000 description 3
- 229910052710 silicon Inorganic materials 0.000 description 3
- 201000010153 skin papilloma Diseases 0.000 description 3
- 239000010802 sludge Substances 0.000 description 3
- 238000010561 standard procedure Methods 0.000 description 3
- 235000000346 sugar Nutrition 0.000 description 3
- 239000002352 surface water Substances 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
- 238000004065 wastewater treatment Methods 0.000 description 3
- MYRTYDVEIRVNKP-UHFFFAOYSA-N 1,2-Divinylbenzene Chemical compound C=CC1=CC=CC=C1C=C MYRTYDVEIRVNKP-UHFFFAOYSA-N 0.000 description 2
- HCGYMSSYSAKGPK-UHFFFAOYSA-N 2-nitro-1h-indole Chemical compound C1=CC=C2NC([N+](=O)[O-])=CC2=C1 HCGYMSSYSAKGPK-UHFFFAOYSA-N 0.000 description 2
- FTBBGQKRYUTLMP-UHFFFAOYSA-N 2-nitro-1h-pyrrole Chemical compound [O-][N+](=O)C1=CC=CN1 FTBBGQKRYUTLMP-UHFFFAOYSA-N 0.000 description 2
- 241000589218 Acetobacteraceae Species 0.000 description 2
- 241001662474 Acidimicrobiaceae Species 0.000 description 2
- 241000290113 Acidithiobacillaceae Species 0.000 description 2
- 241000290116 Acidithiobacillales Species 0.000 description 2
- 241001655301 Acidothermaceae Species 0.000 description 2
- 241000589013 Alcaligenaceae Species 0.000 description 2
- 241000276374 Alcanivoracaceae Species 0.000 description 2
- 241001142141 Aquificae <phylum> Species 0.000 description 2
- 101100206185 Arabidopsis thaliana TCP18 gene Proteins 0.000 description 2
- 101100206195 Arabidopsis thaliana TCP2 gene Proteins 0.000 description 2
- 240000002900 Arthrospira platensis Species 0.000 description 2
- 235000016425 Arthrospira platensis Nutrition 0.000 description 2
- 238000012935 Averaging Methods 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 108700040198 Biotin synthases Proteins 0.000 description 2
- 241000131971 Bradyrhizobiaceae Species 0.000 description 2
- 241000249497 Brucellaceae Species 0.000 description 2
- 241001185363 Chlamydiae Species 0.000 description 2
- 241001425699 Chlorobia Species 0.000 description 2
- 241001425707 Chlorobiaceae Species 0.000 description 2
- 241001425700 Chlorobiales Species 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 241000192699 Chroococcales Species 0.000 description 2
- 241000186031 Corynebacteriaceae Species 0.000 description 2
- 241000246077 Coxiellaceae Species 0.000 description 2
- 239000003298 DNA probe Substances 0.000 description 2
- 241001467894 Desulfovibrionaceae Species 0.000 description 2
- 241000984608 Desulfuromonadales Species 0.000 description 2
- 241001182937 Dictyoglomaceae Species 0.000 description 2
- 241001182939 Dictyoglomales Species 0.000 description 2
- 241001655304 Dietziaceae Species 0.000 description 2
- 108010014468 Dihydrodipicolinate Reductase Proteins 0.000 description 2
- 206010061818 Disease progression Diseases 0.000 description 2
- 108010089072 Dolichyl-diphosphooligosaccharide-protein glycotransferase Proteins 0.000 description 2
- 108700039887 Essential Genes Proteins 0.000 description 2
- 241001279371 Frankiaceae Species 0.000 description 2
- 241001609200 Fulvimarina Species 0.000 description 2
- 241000892911 Geitlerinema Species 0.000 description 2
- 241000520860 Halanaerobiales Species 0.000 description 2
- 241000520863 Halobacteroidaceae Species 0.000 description 2
- 241001248432 Helicobacteraceae Species 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 101000592685 Homo sapiens Meiotic nuclear division protein 1 homolog Proteins 0.000 description 2
- 241000985081 Hyphomonadaceae Species 0.000 description 2
- 229930010555 Inosine Natural products 0.000 description 2
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 2
- 108091023242 Internal transcribed spacer Proteins 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 241000186779 Listeria monocytogenes Species 0.000 description 2
- 102100033679 Meiotic nuclear division protein 1 homolog Human genes 0.000 description 2
- 241000947897 Methylococcales Species 0.000 description 2
- 241000736262 Microbiota Species 0.000 description 2
- 241000204034 Mycoplasmataceae Species 0.000 description 2
- 241000204003 Mycoplasmatales Species 0.000 description 2
- 108091005461 Nucleic proteins Proteins 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 241000192494 Oscillatoriales Species 0.000 description 2
- 241001277521 Oxalobacteraceae Species 0.000 description 2
- 241001112734 Paenibacillaceae Species 0.000 description 2
- 108091093037 Peptide nucleic acid Proteins 0.000 description 2
- 241001112694 Peptococcaceae Species 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 241000425347 Phyla <beetle> Species 0.000 description 2
- 241000982667 Phyllobacteriaceae Species 0.000 description 2
- 241000192135 Prochloraceae Species 0.000 description 2
- ATUOYWHBWRKTHZ-UHFFFAOYSA-N Propane Chemical compound CCC ATUOYWHBWRKTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000947836 Pseudomonadaceae Species 0.000 description 2
- 241000204102 Pseudonocardiaceae Species 0.000 description 2
- 241000192031 Ruminococcus Species 0.000 description 2
- 101100004651 Schizosaccharomyces pombe (strain 972 / ATCC 24843) brc1 gene Proteins 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- 244000166071 Shorea robusta Species 0.000 description 2
- 235000015076 Shorea robusta Nutrition 0.000 description 2
- 101710172711 Structural protein Proteins 0.000 description 2
- 241000282898 Sus scrofa Species 0.000 description 2
- 108700005078 Synthetic Genes Proteins 0.000 description 2
- 241001568368 Syntrophobacteraceae Species 0.000 description 2
- 241000726445 Viroids Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 241000947909 Xanthomonadales Species 0.000 description 2
- XLPNRFXSYBURJM-UHFFFAOYSA-H [U+6].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O Chemical compound [U+6].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XLPNRFXSYBURJM-UHFFFAOYSA-H 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 101150085692 bioC gene Proteins 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 238000007413 biotinylation Methods 0.000 description 2
- 230000006287 biotinylation Effects 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 230000022131 cell cycle Effects 0.000 description 2
- 210000001072 colon Anatomy 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 238000010205 computational analysis Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- DMSZORWOGDLWGN-UHFFFAOYSA-N ctk1a3526 Chemical compound NP(N)(N)=O DMSZORWOGDLWGN-UHFFFAOYSA-N 0.000 description 2
- 101150073654 dapB gene Proteins 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 235000015872 dietary supplement Nutrition 0.000 description 2
- 230000005750 disease progression Effects 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 238000012268 genome sequencing Methods 0.000 description 2
- 230000003862 health status Effects 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 238000000126 in silico method Methods 0.000 description 2
- 229960003786 inosine Drugs 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000002452 interceptive effect Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- DRAVOWXCEBXPTN-UHFFFAOYSA-N isoguanine Chemical class NC1=NC(=O)NC2=C1NC=N2 DRAVOWXCEBXPTN-UHFFFAOYSA-N 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000005304 joining Methods 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 210000005075 mammary gland Anatomy 0.000 description 2
- 230000000873 masking effect Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000001617 migratory effect Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000007837 multiplex assay Methods 0.000 description 2
- 239000002581 neurotoxin Substances 0.000 description 2
- 231100000618 neurotoxin Toxicity 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 210000004789 organ system Anatomy 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000007639 printing Methods 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 230000000529 probiotic effect Effects 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 235000014102 seafood Nutrition 0.000 description 2
- 238000003307 slaughter Methods 0.000 description 2
- 125000006850 spacer group Chemical group 0.000 description 2
- 229940082787 spirulina Drugs 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 230000009182 swimming Effects 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- 239000013077 target material Substances 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 210000001578 tight junction Anatomy 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 229910000163 uranium phosphate Inorganic materials 0.000 description 2
- 238000010200 validation analysis Methods 0.000 description 2
- 229940118696 vibrio cholerae Drugs 0.000 description 2
- 239000002349 well water Substances 0.000 description 2
- 235000020681 well water Nutrition 0.000 description 2
- XQCZBXHVTFVIFE-UHFFFAOYSA-N 2-amino-4-hydroxypyrimidine Chemical class NC1=NC=CC(O)=N1 XQCZBXHVTFVIFE-UHFFFAOYSA-N 0.000 description 1
- ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 description 1
- DBCAQXHNJOFNGC-UHFFFAOYSA-N 4-bromo-1,1,1-trifluorobutane Chemical compound FC(F)(F)CCCBr DBCAQXHNJOFNGC-UHFFFAOYSA-N 0.000 description 1
- 108010042833 7,8-diaminopelargonic acid aminotransferase Proteins 0.000 description 1
- 241000588625 Acinetobacter sp. Species 0.000 description 1
- 241000203716 Actinomycetaceae Species 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 241001112723 Aerococcaceae Species 0.000 description 1
- 241000282979 Alces alces Species 0.000 description 1
- 241000192542 Anabaena Species 0.000 description 1
- 241000149144 Anabaenopsis Species 0.000 description 1
- 241000238421 Arthropoda Species 0.000 description 1
- 241000193738 Bacillus anthracis Species 0.000 description 1
- 102100032487 Beta-mannosidase Human genes 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 101100439426 Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) groEL4 gene Proteins 0.000 description 1
- 241001248433 Campylobacteraceae Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241001112722 Carnobacteriaceae Species 0.000 description 1
- 241000863012 Caulobacter Species 0.000 description 1
- 241000282994 Cervidae Species 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 102000012286 Chitinases Human genes 0.000 description 1
- 108010022172 Chitinases Proteins 0.000 description 1
- 241000606069 Chlamydiaceae Species 0.000 description 1
- 241000498849 Chlamydiales Species 0.000 description 1
- 108020004998 Chloroplast DNA Proteins 0.000 description 1
- 108020005133 Chloroplast RNA Proteins 0.000 description 1
- 241000190834 Chromatiaceae Species 0.000 description 1
- 208000003322 Coinfection Diseases 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 108010051219 Cre recombinase Proteins 0.000 description 1
- 241001032487 Cryomorphaceae Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241001464430 Cyanobacterium Species 0.000 description 1
- 102100021009 Cytochrome b-c1 complex subunit Rieske, mitochondrial Human genes 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- 108010017826 DNA Polymerase I Proteins 0.000 description 1
- 102000004594 DNA Polymerase I Human genes 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 description 1
- 102100033215 DNA nucleotidylexotransferase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 102000007260 Deoxyribonuclease I Human genes 0.000 description 1
- 108010008532 Deoxyribonuclease I Proteins 0.000 description 1
- 241001509319 Desulfitobacterium Species 0.000 description 1
- 241000205085 Desulfobacter Species 0.000 description 1
- 241000186541 Desulfotomaculum Species 0.000 description 1
- 241000605716 Desulfovibrio Species 0.000 description 1
- 241001248482 Ectothiorhodospiraceae Species 0.000 description 1
- 241001468263 Entomoplasmataceae Species 0.000 description 1
- 241001114405 Entomoplasmatales Species 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 241000702191 Escherichia virus P1 Species 0.000 description 1
- OTMSDBZUPAUEDD-UHFFFAOYSA-N Ethane Chemical compound CC OTMSDBZUPAUEDD-UHFFFAOYSA-N 0.000 description 1
- 229920002444 Exopolysaccharide Polymers 0.000 description 1
- 241000206218 Fervidobacterium nodosum Species 0.000 description 1
- 241000589602 Francisella tularensis Species 0.000 description 1
- 241001478286 Francisellaceae Species 0.000 description 1
- 108091092584 GDNA Proteins 0.000 description 1
- 241000168717 Geothrix Species 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 241000350665 Hapalosiphon Species 0.000 description 1
- 244000130592 Hibiscus syriacus Species 0.000 description 1
- 235000018081 Hibiscus syriacus Nutrition 0.000 description 1
- 101000643956 Homo sapiens Cytochrome b-c1 complex subunit Rieske, mitochondrial Proteins 0.000 description 1
- 101001130785 Homo sapiens Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48 kDa subunit Proteins 0.000 description 1
- 101001099199 Homo sapiens RalA-binding protein 1 Proteins 0.000 description 1
- 101001109145 Homo sapiens Receptor-interacting serine/threonine-protein kinase 1 Proteins 0.000 description 1
- 101000640289 Homo sapiens Synemin Proteins 0.000 description 1
- 101000649993 Homo sapiens WW domain-binding protein 1 Proteins 0.000 description 1
- 241000256602 Isoptera Species 0.000 description 1
- 244000199866 Lactobacillus casei Species 0.000 description 1
- 241000246099 Legionellales Species 0.000 description 1
- 241000589901 Leptospiraceae Species 0.000 description 1
- 241001609976 Leuconostocaceae Species 0.000 description 1
- 241001435619 Lile Species 0.000 description 1
- 241000428198 Lutrinae Species 0.000 description 1
- 241001134698 Lyngbya Species 0.000 description 1
- 239000007987 MES buffer Substances 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 238000007476 Maximum Likelihood Methods 0.000 description 1
- 102000000490 Mediator Complex Human genes 0.000 description 1
- 108010080991 Mediator Complex Proteins 0.000 description 1
- 241000589330 Methylococcaceae Species 0.000 description 1
- 241001453272 Methylophilaceae Species 0.000 description 1
- 241000192701 Microcystis Species 0.000 description 1
- 108020005196 Mitochondrial DNA Proteins 0.000 description 1
- 241000589289 Moraxellaceae Species 0.000 description 1
- 241000186360 Mycobacteriaceae Species 0.000 description 1
- 241000202896 Mycoplasma neurolyticum Species 0.000 description 1
- 241000189143 Natronoanaerobium Species 0.000 description 1
- 101100221647 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) cox-1 gene Proteins 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 241001647800 Nocardiopsaceae Species 0.000 description 1
- 241000059630 Nodularia <Cyanobacteria> Species 0.000 description 1
- 241000192656 Nostoc Species 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 240000008881 Oenanthe javanica Species 0.000 description 1
- 241000192134 Oenococcus oeni Species 0.000 description 1
- 241000192497 Oscillatoria Species 0.000 description 1
- 241000283203 Otariidae Species 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000863392 Pelobacter Species 0.000 description 1
- 241000283216 Phocidae Species 0.000 description 1
- 108010004729 Phycoerythrin Proteins 0.000 description 1
- 241000497192 Phyllocoptruta oleivora Species 0.000 description 1
- 241000370518 Phytophthora ramorum Species 0.000 description 1
- 241000589963 Planctomycetaceae Species 0.000 description 1
- 241000530769 Planktothrix Species 0.000 description 1
- 241001112744 Planococcaceae Species 0.000 description 1
- 239000005062 Polybutadiene Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 229920001328 Polyvinylidene chloride Polymers 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 239000006004 Quartz sand Substances 0.000 description 1
- 102000009572 RNA Polymerase II Human genes 0.000 description 1
- 108010009460 RNA Polymerase II Proteins 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 108091036333 Rapid DNA Proteins 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- 241001662468 Rubrobacteraceae Species 0.000 description 1
- 241001662470 Rubrobacterales Species 0.000 description 1
- 241000282849 Ruminantia Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 101100427405 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) RIP1 gene Proteins 0.000 description 1
- 101100512754 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SRB4 gene Proteins 0.000 description 1
- 241000607361 Salmonella enterica subsp. enterica Species 0.000 description 1
- 241001354013 Salmonella enterica subsp. enterica serovar Enteritidis Species 0.000 description 1
- 241000191110 Saprospira grandis Species 0.000 description 1
- 241000970913 Schizothrix Species 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 241000328868 Solibacteres Species 0.000 description 1
- 241001112735 Sporolactobacillaceae Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 102100033920 Synemin Human genes 0.000 description 1
- 108010044281 TATA-Box Binding Protein Proteins 0.000 description 1
- 102000006467 TATA-Box Binding Protein Human genes 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 241001112732 Thermoactinomycetaceae Species 0.000 description 1
- 241000203626 Thermomonosporaceae Species 0.000 description 1
- 241001313706 Thermosynechococcus Species 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 108020004566 Transfer RNA Proteins 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- OKKRPWIIYQTPQF-UHFFFAOYSA-N Trimethylolpropane trimethacrylate Chemical compound CC(=C)C(=O)OCC(CC)(COC(=O)C(C)=C)COC(=O)C(C)=C OKKRPWIIYQTPQF-UHFFFAOYSA-N 0.000 description 1
- 102100028279 WW domain-binding protein 1 Human genes 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 241001660687 Xantho Species 0.000 description 1
- 241001453327 Xanthomonadaceae Species 0.000 description 1
- 230000035508 accumulation Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000003570 air Substances 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- 239000012805 animal sample Substances 0.000 description 1
- 210000003423 ankle Anatomy 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 210000000436 anus Anatomy 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 229940065181 bacillus anthracis Drugs 0.000 description 1
- 230000037429 base substitution Effects 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 108010055059 beta-Mannosidase Proteins 0.000 description 1
- 230000032770 biofilm formation Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000033558 biomineral tissue development Effects 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 239000003876 biosurfactant Substances 0.000 description 1
- 238000004061 bleaching Methods 0.000 description 1
- 239000005420 bog Substances 0.000 description 1
- 235000012206 bottled water Nutrition 0.000 description 1
- 239000001273 butane Substances 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 238000004177 carbon cycle Methods 0.000 description 1
- 235000019994 cava Nutrition 0.000 description 1
- 210000004534 cecum Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000012459 cleaning agent Substances 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 101150061434 cox gene Proteins 0.000 description 1
- 244000000037 crop pathogen Species 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000005202 decontamination Methods 0.000 description 1
- 230000003588 decontaminative effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000003413 degradative effect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 230000014670 detection of bacterium Effects 0.000 description 1
- 108010050073 dethiobiotin synthetase Proteins 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 238000006471 dimerization reaction Methods 0.000 description 1
- 239000004205 dimethyl polysiloxane Substances 0.000 description 1
- NAGJZTKCGNOGPW-UHFFFAOYSA-K dioxido-sulfanylidene-sulfido-$l^{5}-phosphane Chemical compound [O-]P([O-])([S-])=S NAGJZTKCGNOGPW-UHFFFAOYSA-K 0.000 description 1
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000007905 drug manufacturing Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000005518 electrochemistry Effects 0.000 description 1
- 230000003028 elevating effect Effects 0.000 description 1
- STVZJERGLQHEKB-UHFFFAOYSA-N ethylene glycol dimethacrylate Substances CC(=C)C(=O)OCCOC(=O)C(C)=C STVZJERGLQHEKB-UHFFFAOYSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000005713 exacerbation Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 244000078673 foodborn pathogen Species 0.000 description 1
- 229940118764 francisella tularensis Drugs 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 210000005095 gastrointestinal system Anatomy 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000013412 genome amplification Methods 0.000 description 1
- 101150077981 groEL gene Proteins 0.000 description 1
- 101150070420 gyrA gene Proteins 0.000 description 1
- 239000011121 hardwood Substances 0.000 description 1
- 238000007417 hierarchical cluster analysis Methods 0.000 description 1
- 238000003898 horticulture Methods 0.000 description 1
- 244000005702 human microbiome Species 0.000 description 1
- 244000052637 human pathogen Species 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000002440 industrial waste Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000007641 inkjet printing Methods 0.000 description 1
- 229910052816 inorganic phosphate Inorganic materials 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000005055 memory storage Effects 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910001463 metal phosphate Inorganic materials 0.000 description 1
- 231100000783 metal toxicity Toxicity 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 238000010208 microarray analysis Methods 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 108091064355 mitochondrial RNA Proteins 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 230000004001 molecular interaction Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 1
- IJDNQMDRQITEOD-UHFFFAOYSA-N n-butane Chemical compound CCCC IJDNQMDRQITEOD-UHFFFAOYSA-N 0.000 description 1
- OFBQJSOFQDEBGM-UHFFFAOYSA-N n-pentane Natural products CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 101150027931 nifT gene Proteins 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 238000004172 nitrogen cycle Methods 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 238000007826 nucleic acid assay Methods 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 238000002966 oligonucleotide array Methods 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 150000002903 organophosphorus compounds Chemical class 0.000 description 1
- 239000005022 packaging material Substances 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 150000004713 phosphodiesters Chemical class 0.000 description 1
- 150000008300 phosphoramidites Chemical class 0.000 description 1
- 241000512250 phototrophic bacterium Species 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 235000013446 pixi Nutrition 0.000 description 1
- 244000000003 plant pathogen Species 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- 229920000779 poly(divinylbenzene) Polymers 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 239000004584 polyacrylic acid Substances 0.000 description 1
- 229920002239 polyacrylonitrile Polymers 0.000 description 1
- 229920002857 polybutadiene Polymers 0.000 description 1
- 229920001195 polyisoprene Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 229920002689 polyvinyl acetate Polymers 0.000 description 1
- 239000011118 polyvinyl acetate Substances 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 229920002102 polyvinyl toluene Polymers 0.000 description 1
- 239000005033 polyvinylidene chloride Substances 0.000 description 1
- 229920002717 polyvinylpyridine Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000001294 propane Substances 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 230000002685 pulmonary effect Effects 0.000 description 1
- 238000012175 pyrosequencing Methods 0.000 description 1
- 239000002096 quantum dot Substances 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 238000010405 reoxidation reaction Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000004043 responsiveness Effects 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 150000003290 ribose derivatives Chemical group 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 229940110486 salmonella enterica subsp. enterica serovar enteritidis Drugs 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000012056 semi-solid material Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- AWUCVROLDVIAJX-GSVOUGTGSA-N sn-glycerol 3-phosphate Chemical compound OC[C@@H](O)COP(O)(O)=O AWUCVROLDVIAJX-GSVOUGTGSA-N 0.000 description 1
- 101150017120 sod gene Proteins 0.000 description 1
- 101150087539 sodA gene Proteins 0.000 description 1
- 101150018269 sodB gene Proteins 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 238000004174 sulfur cycle Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 238000011269 treatment regimen Methods 0.000 description 1
- 238000012108 two-stage analysis Methods 0.000 description 1
- 241000831562 unclassified Acidobacteria Species 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 235000013618 yogurt Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
- Y02A90/10—Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US22093709P | 2009-06-26 | 2009-06-26 | |
| US61/220,937 | 2009-06-26 | ||
| US25956509P | 2009-11-09 | 2009-11-09 | |
| US61/259,565 | 2009-11-09 | ||
| US31764410P | 2010-03-25 | 2010-03-25 | |
| US61/317,644 | 2010-03-25 | ||
| US34781710P | 2010-05-24 | 2010-05-24 | |
| US61/347,817 | 2010-05-24 | ||
| PCT/US2010/040106 WO2010151842A2 (en) | 2009-06-26 | 2010-06-25 | Methods and systems for phylogenetic analysis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2766312A1 CA2766312A1 (en) | 2010-12-29 |
| CA2766312C true CA2766312C (en) | 2020-04-14 |
Family
ID=43387153
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2766312A Active CA2766312C (en) | 2009-06-26 | 2010-06-25 | Methods and systems for phylogenetic analysis |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US20120165215A1 (enExample) |
| EP (1) | EP2446062B1 (enExample) |
| JP (1) | JP5795579B2 (enExample) |
| CA (1) | CA2766312C (enExample) |
| DK (1) | DK2446062T3 (enExample) |
| SG (1) | SG177366A1 (enExample) |
| WO (1) | WO2010151842A2 (enExample) |
Families Citing this family (74)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2700749C (en) * | 2007-10-05 | 2016-03-22 | Exxonmobil Upstream Research Company | Method and apparatus for detection of a liquid under a surface |
| US9228240B2 (en) | 2010-06-03 | 2016-01-05 | California Institute Of Technology | Methods for detecting and quantifying viable bacterial endo-spores |
| EP2665815B1 (en) * | 2011-01-17 | 2017-08-09 | Life Technologies Corporation | Enzymatic ligation of nucleic acids |
| ES2728743T3 (es) | 2011-01-17 | 2019-10-28 | Life Technologies Corp | Flujo de trabajo para la detección de ligandos utilizando ácidos nucleicos |
| US8712712B2 (en) * | 2011-03-29 | 2014-04-29 | Bio-Rad Laboratories, Inc. | System and method for producing statistically valid assay means and ranges for quality control materials |
| WO2013032744A2 (en) | 2011-08-17 | 2013-03-07 | Nume Health, Llc | Composition and use of a formulation to increase the ratio of gastrointestinal microbiota in phylum bacteriodites to microbiota of firmuctes phylum |
| RU2613219C2 (ru) | 2011-11-11 | 2017-03-15 | Эксонмобил Апстрим Рисерч Компани | Способ наблюдения за коллектором с использованием данных о скученных изотопах и/или инертных газах |
| AU2012335021B2 (en) | 2011-11-11 | 2015-08-20 | Exxonmobil Upstream Research Company | Exploration method and system for detection of hydrocarbons |
| FR2985519B1 (fr) * | 2012-01-10 | 2016-02-19 | Biomerieux Sa | Procede de detection et d'identification directe par voie optique d'un microorganisme dans un echantillon biologique |
| US9725770B2 (en) | 2012-03-06 | 2017-08-08 | The Regents Of The University Of California | Methods and compositions for identification of source of microbial contamination in a sample |
| US9092401B2 (en) | 2012-10-31 | 2015-07-28 | Counsyl, Inc. | System and methods for detecting genetic variation |
| ES2637538T3 (es) | 2012-07-17 | 2017-10-13 | Counsyl, Inc. | Sistema y métodos para la detección de una variación genética |
| JP2014023434A (ja) * | 2012-07-24 | 2014-02-06 | Lotte Co Ltd | デンタルプラークの細菌叢の解析方法 |
| EP3435084B1 (en) * | 2012-08-16 | 2023-02-22 | Decipher Biosciences, Inc. | Prostate cancer prognostics using biomarkers |
| EP3628161B1 (en) | 2012-11-23 | 2023-04-05 | Seres Therapeutics, Inc. | Synergistic bacterial compositions and methods of production and use thereof |
| US8906668B2 (en) | 2012-11-23 | 2014-12-09 | Seres Health, Inc. | Synergistic bacterial compositions and methods of production and use thereof |
| US20140179554A1 (en) * | 2012-12-21 | 2014-06-26 | Hutman Diagnostics AG | Microarrays |
| EP2941259A4 (en) | 2013-01-04 | 2016-07-27 | Second Genome Inc | MIKROBIOMMODULATIONSINDEX |
| CA2899926C (en) | 2013-02-04 | 2025-11-18 | Seres Therapeutics, Inc. | DIGESTIVE TUBE FILLING COMPOSITIONS |
| WO2014121304A1 (en) | 2013-02-04 | 2014-08-07 | Seres Health, Inc. | Compositions and methods |
| EP2972371B1 (en) | 2013-03-14 | 2018-09-05 | Second Genome, Inc. | Microbial ecology shift assay |
| WO2014152281A2 (en) * | 2013-03-15 | 2014-09-25 | The Broad Institute, Inc. | Ribosomal ribonucleic acid hybridization for organism identification |
| EP2967077A4 (en) | 2013-03-15 | 2016-09-14 | Seres Therapeutics Inc | NETWORK-BASED MICROBIAL COMPOSITIONS AND METHOD |
| WO2014186471A1 (en) * | 2013-05-15 | 2014-11-20 | John Hulls | Composting system and method |
| MX367109B (es) | 2013-11-25 | 2019-08-05 | Seres Therapeutics Inc | Composiciones bacterianas sinergicas y sus metodos de produccion y usos. |
| EA201691157A1 (ru) | 2013-12-04 | 2016-09-30 | Эксонмобил Апстрим Рисерч Компани | Способ и система для обнаружения материала в области земли |
| WO2015095241A2 (en) | 2013-12-16 | 2015-06-25 | Seres Health, Inc. | Bacterial compositions and methods of use thereof for treatment of immune system disorders |
| US11047232B2 (en) | 2013-12-31 | 2021-06-29 | Biota Technology, Inc | Microbiome based systems, apparatus and methods for the exploration and production of hydrocarbons |
| US11028449B2 (en) | 2013-12-31 | 2021-06-08 | Biota Technology, Inc. | Microbiome based systems, apparatus and methods for monitoring and controlling industrial processes and systems |
| WO2015103165A1 (en) * | 2013-12-31 | 2015-07-09 | Biota Technology, Inc. | Microbiome based systems, apparatus and methods for monitoring and controlling industrial processes and systems |
| US9387451B2 (en) | 2014-02-03 | 2016-07-12 | International Business Machines Corporation | Flow cell array and uses thereof |
| EP3114505B1 (en) | 2014-03-07 | 2022-07-20 | ExxonMobil Upstream Research Company | Exploration method and system for detection of hydrocarbons from the water column |
| CA2944896A1 (en) * | 2014-04-11 | 2015-10-15 | The Trustees Of The University Of Pennsylvania | Compositions and methods for metagenome biomarker detection |
| WO2015171493A1 (en) * | 2014-05-04 | 2015-11-12 | Salix Pharmaceuticals, Inc. | Ibs microbiota and uses thereof |
| WO2015171834A1 (en) * | 2014-05-06 | 2015-11-12 | Phylagen, Inc. | Monitoring and managing a facility microbiome |
| US9453828B2 (en) | 2014-07-18 | 2016-09-27 | Exxonmobil Upstream Research Company | Method and system for identifying and sampling hydrocarbons with buoys |
| AU2015290137B2 (en) | 2014-07-18 | 2018-03-01 | Exxonmobil Upstream Research Company | Method and system for identifying and sampling hydrocarbons |
| US9638828B2 (en) | 2014-07-18 | 2017-05-02 | Exxonmobil Upstream Research Company | Method and system for performing surveying and sampling in a body of water |
| US10410749B2 (en) | 2014-10-21 | 2019-09-10 | uBiome, Inc. | Method and system for microbiome-derived characterization, diagnostics and therapeutics for cutaneous conditions |
| WO2016065075A1 (en) | 2014-10-21 | 2016-04-28 | uBiome, Inc. | Method and system for microbiome-derived diagnostics and therapeutics |
| JP6514549B2 (ja) * | 2015-04-03 | 2019-05-15 | 住友化学株式会社 | 微生物叢解析システム、判定システム、微生物叢解析方法及び判定方法 |
| EP3283084B1 (en) * | 2015-04-13 | 2025-08-06 | Psomagen, Inc. | Method and system for microbiome-derived characterization, diagnostics and therapeutics for cutaneous conditions |
| JP6643815B2 (ja) * | 2015-05-15 | 2020-02-12 | 森永乳業株式会社 | 腸内状態の判定方法、および腸内状態の判定装置 |
| US20180357365A1 (en) * | 2015-10-02 | 2018-12-13 | Phylagen, Inc. | Product authentication and tracking |
| US10724108B2 (en) | 2016-05-31 | 2020-07-28 | Exxonmobil Upstream Research Company | Methods for isolating nucleic acids from samples |
| US10496695B2 (en) * | 2016-06-06 | 2019-12-03 | Think-Cell Software Gmbh | Automated data extraction from scatter plot images |
| WO2017214557A1 (en) | 2016-06-10 | 2017-12-14 | Counsyl, Inc. | Nucleic acid sequencing adapters and uses thereof |
| WO2018005514A1 (en) | 2016-07-01 | 2018-01-04 | Exxonmobil Upstream Research Company | Methods to determine conditions of a hydrocarbon reservoir |
| US20180030403A1 (en) | 2016-07-28 | 2018-02-01 | Bobban Subhadra | Devices, systems and methods for the production of humanized gut commensal microbiota |
| US11959125B2 (en) * | 2016-09-15 | 2024-04-16 | Sun Genomics, Inc. | Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample |
| CA3037366A1 (en) | 2016-09-29 | 2018-04-05 | Myriad Women's Health, Inc. | Noninvasive prenatal screening using dynamic iterative depth optimization |
| US11015154B2 (en) | 2016-11-09 | 2021-05-25 | The Regents Of The University Of California | Methods for identifying interactions amongst microorganisms |
| US10968447B2 (en) | 2017-01-31 | 2021-04-06 | Myriad Women's Health, Inc. | Methods and compositions for enrichment of target polynucleotides |
| US10752946B2 (en) | 2017-01-31 | 2020-08-25 | Myriad Women's Health, Inc. | Methods and compositions for enrichment of target polynucleotides |
| US11232850B2 (en) | 2017-03-24 | 2022-01-25 | Myriad Genetics, Inc. | Copy number variant caller |
| CN111212655A (zh) | 2017-08-14 | 2020-05-29 | 赛里斯治疗公司 | 用于治疗胆汁淤积性疾病的组合物和方法 |
| WO2019084245A1 (en) | 2017-10-27 | 2019-05-02 | Myriad Women's Health, Inc. | METHODS AND COMPOSITIONS FOR PREPARING NUCLEIC ACID LIBRARIES |
| JP2021501185A (ja) | 2017-10-30 | 2021-01-14 | セレス セラピューティクス インコーポレイテッド | 抗生物質耐性を処置するための組成物及び方法 |
| JP6995604B2 (ja) * | 2017-12-15 | 2022-01-14 | 東洋鋼鈑株式会社 | 一塩基多型検出用プローブの設計方法及びプローブセット |
| CN109935376B (zh) * | 2017-12-18 | 2022-06-28 | 中国核动力研究设计院 | 一种核电厂严重事故下β射线对仪表损伤的量化方法 |
| US11525164B2 (en) | 2018-03-27 | 2022-12-13 | The Trustees Of Columbia University In The City Of New York | Spatial metagenomic characterization of microbial biogeography |
| CN109113732B (zh) * | 2018-08-09 | 2022-03-29 | 中国石油天然气股份有限公司 | 储层非均质性的确定方法及装置 |
| CN109270032B (zh) * | 2018-08-20 | 2021-08-20 | 杭州腾海科技有限公司 | 一种水体藻类在线监测装置 |
| CN109273053B (zh) * | 2018-09-27 | 2021-10-08 | 华中科技大学鄂州工业技术研究院 | 一种高通量测序的微生物数据处理方法 |
| WO2020245785A2 (en) * | 2019-06-06 | 2020-12-10 | Tata Consultancy Services Limited | Method and system for identification of candidate target sites for combating pathogens |
| CN110484637B (zh) * | 2019-09-19 | 2020-06-09 | 南京大学 | 一种水体粪便污染检测的引物、试剂盒及高通量溯源方法 |
| US10942165B1 (en) * | 2019-12-20 | 2021-03-09 | Dnae Group Holdings Ltd | Methods for preparing cartridges for in vitro diagnostics and related systems |
| KR102676313B1 (ko) * | 2020-02-27 | 2024-06-17 | 경희대학교 산학협력단 | 유전자 선별 방법 및 장치 |
| CN111477276B (zh) * | 2020-04-02 | 2020-12-15 | 上海之江生物科技股份有限公司 | 微生物的种特异共有序列的获得方法、装置及应用 |
| CN111847778A (zh) * | 2020-07-15 | 2020-10-30 | 北京天诚众合科技发展有限公司 | 一种景观水体、坑塘治理维护的方法 |
| JP7414029B2 (ja) * | 2021-02-26 | 2024-01-16 | 横河電機株式会社 | 測定方法及び測定システム |
| CN113096734B (zh) * | 2021-05-11 | 2021-12-14 | 中国科学院水生生物研究所 | 一种筛选用于二倍体群体亲子鉴定的分子标记组合的方法 |
| CN117473398B (zh) * | 2023-12-26 | 2024-03-19 | 四川国蓝中天环境科技集团有限公司 | 一种基于运渣车活动的城市扬尘污染源分类方法 |
| CN117497065B (zh) * | 2023-12-28 | 2024-04-02 | 中国农业大学 | 筛选促进多年生牧草再生的微生物种类的方法及其所用装置与计算机可读存储介质 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7115364B1 (en) * | 1993-10-26 | 2006-10-03 | Affymetrix, Inc. | Arrays of nucleic acid probes on biological chips |
| AU1337099A (en) * | 1997-10-29 | 1999-05-17 | Mira Diagnostica Gmbh | Method for identifying micro-organisms |
| US7955794B2 (en) * | 2000-09-21 | 2011-06-07 | Illumina, Inc. | Multiplex nucleic acid reactions |
| US20020187490A1 (en) * | 2001-06-07 | 2002-12-12 | Michigan State University | Microbial identification chip based on DNA-DNA hybridization |
| JP2005512527A (ja) * | 2001-12-11 | 2005-05-12 | アフィメトリックス インコーポレイテッド | 転写活性を決定するための方法 |
| JP2004357550A (ja) * | 2003-06-03 | 2004-12-24 | Sankyo Co Ltd | 遺伝子発現データの解析方法 |
| US20050112636A1 (en) * | 2003-09-23 | 2005-05-26 | Atom Sciences | Polymeric nucleic acid hybridization probes |
| US7323308B2 (en) * | 2004-09-03 | 2008-01-29 | Affymetrix, Inc. | Methods of genetic analysis of E. coli |
| AU2005335216A1 (en) * | 2004-10-05 | 2007-02-15 | Wyeth | Probe arrays for detecting multiple strains of different species |
| JP2007060953A (ja) * | 2005-08-30 | 2007-03-15 | Hitachi Ltd | 細菌叢の分析方法 |
| EP1770171A1 (en) * | 2005-09-29 | 2007-04-04 | Universität Zu Köln | DNA microarray for rapid identification of Candida albicans in blood cultures. |
| WO2007056680A2 (en) * | 2005-11-03 | 2007-05-18 | Forsyth Dental Infirmary For Children | Methods and arrays for identifying human microflora |
| US20070122831A1 (en) * | 2005-11-12 | 2007-05-31 | Dave Bachoon | Detection and source identification of microbial contaminants in water samples |
| KR100813263B1 (ko) * | 2006-08-17 | 2008-03-13 | 삼성전자주식회사 | 표적 서열 검출용 프로브 설계 방법 및 상기 프로브를이용한 표적 서열 검출 방법 |
| DK2099935T3 (da) * | 2006-11-30 | 2014-04-07 | Univ California | Array for detektion af mikrober |
| US8080372B2 (en) * | 2007-04-11 | 2011-12-20 | Canon Kabushiki Kaisha | Method for detecting nucleic acid in sample, method for designing probes, system for designing probes therefor |
-
2010
- 2010-06-25 DK DK10792771.7T patent/DK2446062T3/da active
- 2010-06-25 CA CA2766312A patent/CA2766312C/en active Active
- 2010-06-25 US US13/379,839 patent/US20120165215A1/en not_active Abandoned
- 2010-06-25 EP EP10792771.7A patent/EP2446062B1/en active Active
- 2010-06-25 JP JP2012517804A patent/JP5795579B2/ja active Active
- 2010-06-25 WO PCT/US2010/040106 patent/WO2010151842A2/en not_active Ceased
- 2010-06-25 SG SG2011096260A patent/SG177366A1/en unknown
-
2018
- 2018-03-02 US US15/910,905 patent/US20180223339A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20120165215A1 (en) | 2012-06-28 |
| WO2010151842A3 (en) | 2011-03-03 |
| CA2766312A1 (en) | 2010-12-29 |
| WO2010151842A2 (en) | 2010-12-29 |
| EP2446062B1 (en) | 2014-08-13 |
| SG177366A1 (en) | 2012-02-28 |
| DK2446062T3 (da) | 2014-10-06 |
| JP2012531211A (ja) | 2012-12-10 |
| EP2446062A2 (en) | 2012-05-02 |
| JP5795579B2 (ja) | 2015-10-14 |
| EP2446062A4 (en) | 2013-03-20 |
| US20180223339A1 (en) | 2018-08-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2766312C (en) | Methods and systems for phylogenetic analysis | |
| Debroas et al. | Evidence for an active rare biosphere within freshwater protists community | |
| Brown et al. | Selection and horizontal gene transfer underlie microdiversity-level heterogeneity in resistance gene fate during wastewater treatment | |
| Tan et al. | Next-generation sequencing (NGS) for assessment of microbial water quality: current progress, challenges, and future opportunities | |
| US20120264637A1 (en) | Methods and systems for phylogenetic analysis | |
| McLellan et al. | Discovering new indicators of fecal pollution | |
| US10961593B2 (en) | Methods and compositions for identification of source of microbial contamination in a sample | |
| Xu et al. | Fungal diversity in deep-sea sediments of a hydrothermal vent system in the Southwest Indian Ridge | |
| US20140155283A1 (en) | Microarray for detecting viable organisms | |
| Jia et al. | Sequencing introduced false positive rare taxa lead to biased microbial community diversity, assembly, and interaction interpretation in amplicon studies | |
| Huang et al. | Estuarine salinity gradient governs sedimentary bacterial community but not antibiotic resistance gene profile | |
| Dam et al. | Targeted cell sorting combined with single cell genomics captures low abundant microbial dark matter with higher sensitivity than metagenomics | |
| Wang et al. | Absolute quantification of enterococcal 23S rRNA gene using digital PCR | |
| Valeriani et al. | The microbiota as a candidate biomarker for SPA pools and SPA thermal spring stability after seismic events | |
| Cabrol et al. | Redox gradient shapes the abundance and diversity of mercury-methylating microorganisms along the water column of the Black Sea | |
| Brown et al. | Seasonality and distribution of cyanobacteria and microcystin toxin genes in an oligotrophic lake of Atlantic Canada | |
| Shu et al. | CyanoStrainChip: A Novel DNA Microarray Tool for High-Throughput Detection of Environmental Cyanobacteria at the Strain Level | |
| Galagoda et al. | Quantitative 16S rRNA gene amplicon sequencing for comprehensive pathogenic bacterial tracking in a municipal wastewater treatment plant | |
| Nasreen et al. | Dynamic subspecies population structure of Vibrio cholerae in Dhaka, Bangladesh | |
| Andersen | The use of microarrays in microbial ecology | |
| Islam et al. | Dynamic subspecies population structure of Vibrio cholerae in Dhaka, Bangladesh | |
| Cao et al. | Community analysis-based methods | |
| Pruden et al. | Selective agents, microbial ecology, and horizontal gene transfer underly the fate of resistance genes during biological wastewater treatment | |
| Koh | Diversity of Antibiotic Resistance Genes in the Waters of Greater Pearl River Delta: Application as Pollution Indicator | |
| Durstock et al. | Temporal changes in fecal indicator bacteria and potential waterborne pathogens at Sequiota Spring: from sewer to spring |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20150113 |