CA2759465C - Method of producing polymers of spider silk proteins - Google Patents
Method of producing polymers of spider silk proteins Download PDFInfo
- Publication number
- CA2759465C CA2759465C CA2759465A CA2759465A CA2759465C CA 2759465 C CA2759465 C CA 2759465C CA 2759465 A CA2759465 A CA 2759465A CA 2759465 A CA2759465 A CA 2759465A CA 2759465 C CA2759465 C CA 2759465C
- Authority
- CA
- Canada
- Prior art keywords
- amino acid
- acid residues
- spider silk
- liquid medium
- silk protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
- C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Insects & Arthropods (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Toxicology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP09158445.8 | 2009-04-22 | ||
| EP09158445A EP2243792A1 (en) | 2009-04-22 | 2009-04-22 | Methods of producing polymers of spider silk proteins |
| EP10156927.5 | 2010-03-18 | ||
| EP10156923.4 | 2010-03-18 | ||
| EP10156923 | 2010-03-18 | ||
| EP10156927 | 2010-03-18 | ||
| PCT/SE2010/050439 WO2010123450A1 (en) | 2009-04-22 | 2010-04-21 | Method of producing polymers of spider silk proteins |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2759465A1 CA2759465A1 (en) | 2010-10-28 |
| CA2759465C true CA2759465C (en) | 2017-12-12 |
Family
ID=43011348
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2759465A Expired - Fee Related CA2759465C (en) | 2009-04-22 | 2010-04-21 | Method of producing polymers of spider silk proteins |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US8642734B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP2421890B1 (cg-RX-API-DMAC7.html) |
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| JP5726282B2 (ja) | 2010-03-18 | 2015-05-27 | スパイバー テクノロジーズ アーベーＳｐｉｂｅｒ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ａｂ | タンパク質及びポリペプチドの産生 |
| CA2815267C (en) | 2010-10-27 | 2019-06-25 | Spiber Technologies Ab | Spider silk fusion protein structures for binding to an organic target |
| US9617315B2 (en) | 2011-06-01 | 2017-04-11 | Spiber Inc. | Artificial polypeptide fiber and method for producing the same |
| CA2872056A1 (en) * | 2012-05-02 | 2013-11-07 | Spiber Technologies Ab | Spider silk fusion protein structures without repetitive fragment for binding to an organic target |
| ES2887366T3 (es) | 2013-09-17 | 2021-12-22 | Bolt Threads Inc | Métodos y composiciones para sintetizar fibras de seda mejoradas |
| AU2015358537B2 (en) | 2014-12-02 | 2021-08-19 | Evolved By Nature, Inc. | Silk performance apparel and products and methods of preparing the same |
| CN114000218A (zh) * | 2015-03-16 | 2022-02-01 | 保尔特纺织品公司 | 改善的丝纤维 |
| CN107849100B (zh) | 2015-04-09 | 2022-02-08 | 丝芭博株式会社 | 极性溶剂溶液及其制造方法 |
| JP6856828B2 (ja) | 2015-04-09 | 2021-04-14 | Ｓｐｉｂｅｒ株式会社 | 極性溶媒溶液及びその製造方法 |
| EP3307765B1 (en) * | 2015-06-11 | 2024-04-10 | Bolt Threads, Inc. | Recombinant protein fiber yarns with improved properties |
| CN105031723B (zh) * | 2015-06-23 | 2017-05-24 | 上海交通大学 | 基于蜘蛛丝蛋白的温敏性水凝胶 |
| BR112018000699B1 (pt) | 2015-07-14 | 2023-10-17 | Silk Therapeutics, Inc | Artigo e método para revestir um material |
| JP2018159137A (ja) | 2015-08-20 | 2018-10-11 | 国立研究開発法人理化学研究所 | クモ糸様構造を有するポリペプチド繊維の製造方法 |
| EP3374378B1 (en) | 2015-11-13 | 2021-02-17 | Spiber Technologies AB | Charge-reversed n-terminal spider silk protein domain and uses thereof |
| GB201522610D0 (en) * | 2015-12-22 | 2016-02-03 | Univ Southampton | Protein |
| WO2017198655A1 (en) * | 2016-05-16 | 2017-11-23 | Spiber Technologies Ab | Spider silk coating of solid surfaces |
| JP2019518882A (ja) * | 2016-06-10 | 2019-07-04 | ボルト スレッズ インコーポレイテッド | 改善された特性を有する組換えタンパク質繊維糸 |
| EP3263593A1 (en) | 2016-07-01 | 2018-01-03 | Anna Rising | Engineered spider silk proteins and uses thereof |
| KR20190046993A (ko) | 2016-09-14 | 2019-05-07 | 볼트 쓰레즈, 인크. | 긴 균일한 재조합 단백질 섬유 |
| US20190275193A1 (en) * | 2016-11-11 | 2019-09-12 | Amsilk Gmbh | Use of a shrinkable biopolymer fiber as a sensor |
| CN107475807A (zh) * | 2017-08-30 | 2017-12-15 | 常州豫春化工有限公司 | 一种改性尼龙6纤维的制备方法 |
| KR102609621B1 (ko) * | 2017-09-26 | 2023-12-01 | 스파이버 테크놀러지스 에이비 | 표면 활성 거대분자의 구조화 |
| EP3688018A4 (en) | 2017-09-27 | 2021-07-07 | Evolved by Nature, Inc. | SILK COVERED FABRICS, RELATED PRODUCTS AND PREPARATION PROCESSES |
| JP2021035330A (ja) * | 2017-09-29 | 2021-03-04 | Ｓｐｉｂｅｒ株式会社 | 発現カセット |
| BR112021026217A2 (pt) * | 2019-07-04 | 2022-02-15 | Seevix Mat Sciences Ltd | Sistema de expressão procariótica e métodos de uso do mesmo. |
| CN110551194B (zh) * | 2019-09-19 | 2023-03-21 | 天津大学 | 重组蜘蛛卵鞘丝蛋白复合物及其生成的人造卵鞘丝 |
| BR112022020937A2 (pt) | 2020-04-23 | 2022-12-20 | Seevix Mat Sciences Ltd | Composições cosméticas compreendendo seda de aranha dragon sintética. |
| WO2024091831A2 (en) * | 2022-10-28 | 2024-05-02 | National Taiwan University | A recombinant spider silk protein and method for tag-free and time-saving purification thereof |
| CN218413490U (zh) | 2022-10-31 | 2023-01-31 | 徐州龙安电子科技有限公司 | 一种新型蜘蛛电子标签 |
| DE102023105437B3 (de) | 2023-03-06 | 2024-06-13 | Dr. Ing. H.C. F. Porsche Aktiengesellschaft | Fahrzeugkomponente mit wenigstens einer Fahrzeuginnenraumkomponente |
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| US20070260039A1 (en) | 2002-01-11 | 2007-11-08 | Karatzas Costas N | Methods of Producing Silk Polypeptides and Products Thereof |
| CN101018806B (zh) * | 2004-07-22 | 2014-05-07 | Am丝绸有限责任公司 | 重组蜘蛛丝蛋白 |
| RU2444527C2 (ru) * | 2005-12-30 | 2012-03-10 | Спибер Текнолоджиз Аб | Рекомбинантный спидроиновый белок главной ампулярной железы, способный к полимеризации, рекомбинантный слитый белок, способный к управляемой полимеризации, полимер спидроинового белка главной ампулярной железы, композиция для управляемой полимеризации, рекомбинантная молекула нуклеиновой кислоты (варианты), способ получения растворимого слитого белка, способ получения полимера спидроинового белка главной ампулярной железы и способ культивирования эукариотических клеток |
| BRPI0701826B1 (pt) * | 2007-03-16 | 2021-02-17 | Embrapa - Empresa Brasileira De Pesquisa Agropecuária | proteínas da teia de aranha nephilengys cruentata, avicularia juruensis e parawixia bistriata isoladas da biodiversidade brasileira |
| US9051383B2 (en) * | 2007-06-11 | 2015-06-09 | The Regents Of The University Of California | Spider silk dragline polynucleotides, polypeptides and methods of use thereof |
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| CN102395601A (zh) | 2012-03-28 |
| EP2421890A1 (en) | 2012-02-29 |
| US8642734B2 (en) | 2014-02-04 |
| RU2011145429A (ru) | 2013-05-27 |
| DK2421890T3 (en) | 2015-11-23 |
| JP5912077B2 (ja) | 2016-04-27 |
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