CA2747312C - Epigenetic markers for the identification of cd3/cd4+ or cd3/cd8+ t-lymphocytes - Google Patents

Epigenetic markers for the identification of cd3/cd4+ or cd3/cd8+ t-lymphocytes Download PDF

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Publication number
CA2747312C
CA2747312C CA2747312A CA2747312A CA2747312C CA 2747312 C CA2747312 C CA 2747312C CA 2747312 A CA2747312 A CA 2747312A CA 2747312 A CA2747312 A CA 2747312A CA 2747312 C CA2747312 C CA 2747312C
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Prior art keywords
lymphocytes
methylation
cpg
seq
amplicon
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CA2747312A
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English (en)
French (fr)
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CA2747312A1 (en
Inventor
Sven Olek
Ivana Tuerbachova
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Precision for Medicine GmbH
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Epiontis GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2747312A 2008-12-16 2009-12-08 Epigenetic markers for the identification of cd3/cd4+ or cd3/cd8+ t-lymphocytes Active CA2747312C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20080021838 EP2199411B1 (en) 2008-12-16 2008-12-16 Epigenetic markers for the identification of CD3 positive T-lymphocytes
EP08021838.1 2008-12-16
PCT/EP2009/008764 WO2010069499A2 (en) 2008-12-16 2009-12-08 Epigenetic markers for the identification of blood sub-cells of type 1

Publications (2)

Publication Number Publication Date
CA2747312A1 CA2747312A1 (en) 2010-06-24
CA2747312C true CA2747312C (en) 2018-11-06

Family

ID=40428037

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2747312A Active CA2747312C (en) 2008-12-16 2009-12-08 Epigenetic markers for the identification of cd3/cd4+ or cd3/cd8+ t-lymphocytes

Country Status (8)

Country Link
US (1) US20120107810A1 (https=)
EP (2) EP2199411B1 (https=)
JP (1) JP2012511917A (https=)
CA (1) CA2747312C (https=)
DK (2) DK2199411T3 (https=)
ES (1) ES2606350T3 (https=)
PL (1) PL2376648T3 (https=)
WO (1) WO2010069499A2 (https=)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170233807A1 (en) 2008-12-16 2017-08-17 Epiontis Gmbh Epigenetic Markers for the Identification of Blood Sub-cells of Type 1
US20130281304A1 (en) * 2010-08-13 2013-10-24 Andrew P. Feinberg Comprehensive Methylome Map of Myeloid and Lymphoid Commitment from Hematopoietic Proenitors
WO2012045888A1 (en) 2010-10-08 2012-04-12 Epiontis Gmbh Method for determining cancer patient survival based on analyzing tumor-infiltrating overall t-lymphocytes
JP2014505475A (ja) * 2011-01-20 2014-03-06 ユニヴェルシテ リブル ドゥ ブリュッセル ヒトの乳癌のエピジェネティックな肖像
US20140178348A1 (en) 2011-05-25 2014-06-26 The Regents Of The University Of California Methods using DNA methylation for identifying a cell or a mixture of cells for prognosis and diagnosis of diseases, and for cell remediation therapies
CA2869295A1 (en) * 2011-05-25 2012-11-29 Brown University Methods using dna methylation for identifying a cell or a mixture of cells for prognosis and diagnosis of diseases, and for cell remediation therapies
WO2013135454A1 (en) * 2012-03-12 2013-09-19 Epiontis Gmbh Epigenetic marker for the identification of cd3cd4 positive t lymphocytes
GB201221133D0 (en) * 2012-11-23 2013-01-09 Epiontis Gmbh Epigenetic method for the identification of subpopulations of CD8 and T lympocytes, in particular CD8 alpha and beta T lymphocytes
JP6590790B2 (ja) 2013-04-19 2019-10-16 エピオンティス ゲーエムベーハー 生体サンプルにおける定量的細胞組成を特定する方法
EP3360971A1 (en) * 2017-02-09 2018-08-15 Epiontis GmbH Improved method for epigenetic immune cell counting
EP3382033B1 (en) * 2017-03-30 2020-08-05 Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen Method for determining blood counts based on dna methylation
DE102017125019B4 (de) * 2017-10-25 2019-10-17 Epiontis Gmbh PDCD1 als epigenetischer Marker zur Identifizierung von Immunzellen, insbesondere PD1+ Zellen
DE102018116353B4 (de) * 2018-07-05 2020-06-10 Epiontis Gmbh Verfahren zum Nachweis und zur Zählung epigenetischer Immunzellen in humanen Blutproben für Immundiagnostik und Neugeborenen-Screening
CN116949156B (zh) * 2023-09-19 2023-12-08 美迪西普亚医药科技(上海)有限公司 一种基于核酸变体的通用型检测人t细胞的分析方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002171973A (ja) * 2000-12-07 2002-06-18 Univ Tokyo Ｄｎａメチル化パターンによる細胞の同定法
WO2004050706A2 (en) 2002-12-03 2004-06-17 Medical Research Council Regulatory t-cells
ES2367012T3 (es) 2006-02-28 2011-10-27 Charite-Universitätsmedizin Berlin Detección y control de calidad de linfocitos t reguladores a través del análisis de metilación del adn del gen foxp3.

Also Published As

Publication number Publication date
EP2199411B1 (en) 2015-05-06
HK1163189A1 (zh) 2012-09-07
DK2199411T3 (en) 2015-08-03
WO2010069499A3 (en) 2010-08-26
WO2010069499A2 (en) 2010-06-24
PL2376648T3 (pl) 2017-05-31
DK2376648T3 (da) 2017-01-02
CA2747312A1 (en) 2010-06-24
US20120107810A1 (en) 2012-05-03
EP2376648A2 (en) 2011-10-19
JP2012511917A (ja) 2012-05-31
ES2606350T3 (es) 2017-03-23
EP2376648B1 (en) 2016-09-14
EP2199411A1 (en) 2010-06-23

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