CA2730565A1 - Detection assays and use thereof - Google Patents
Detection assays and use thereof Download PDFInfo
- Publication number
- CA2730565A1 CA2730565A1 CA2730565A CA2730565A CA2730565A1 CA 2730565 A1 CA2730565 A1 CA 2730565A1 CA 2730565 A CA2730565 A CA 2730565A CA 2730565 A CA2730565 A CA 2730565A CA 2730565 A1 CA2730565 A1 CA 2730565A1
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- Prior art keywords
- oligonucleotide
- sequence
- binding
- reporter
- seq
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- CWERGRDVMFNCDR-UHFFFAOYSA-M thioglycolate(1-) Chemical group [O-]C(=O)CS CWERGRDVMFNCDR-UHFFFAOYSA-M 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- MNRILEROXIRVNJ-UHFFFAOYSA-N tioguanine Chemical compound N1C(N)=NC(=S)C2=NC=N[C]21 MNRILEROXIRVNJ-UHFFFAOYSA-N 0.000 description 1
- 229960003087 tioguanine Drugs 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 125000002221 trityl group Chemical group [H]C1=C([H])C([H])=C([H])C([H])=C1C([*])(C1=C(C(=C(C(=C1[H])[H])[H])[H])[H])C1=C([H])C([H])=C([H])C([H])=C1[H] 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- HDZZVAMISRMYHH-KCGFPETGSA-N tubercidin Chemical compound C1=CC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O HDZZVAMISRMYHH-KCGFPETGSA-N 0.000 description 1
- 102000003298 tumor necrosis factor receptor Human genes 0.000 description 1
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
- 229940045145 uridine Drugs 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- G01N2333/91205—Phosphotransferases in general
- G01N2333/9121—Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases
- G01N2333/91215—Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases with a definite EC number (2.7.1.-)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US96233307P | 2007-07-27 | 2007-07-27 | |
| US60/962,333 | 2007-07-27 | ||
| PCT/US2008/070643 WO2009018003A2 (en) | 2007-07-27 | 2008-07-21 | Detection assays and use thereof |
Publications (1)
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| CA2730565A1 true CA2730565A1 (en) | 2009-02-05 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2730565A Abandoned CA2730565A1 (en) | 2007-07-27 | 2008-07-21 | Detection assays and use thereof |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20100159446A1 (enExample) |
| EP (1) | EP2191268A2 (enExample) |
| JP (1) | JP2010534836A (enExample) |
| CN (1) | CN101952719A (enExample) |
| AU (1) | AU2008282557A1 (enExample) |
| CA (1) | CA2730565A1 (enExample) |
| WO (1) | WO2009018003A2 (enExample) |
Families Citing this family (44)
| Publication number | Priority date | Publication date | Assignee | Title |
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| EP1401850A1 (en) | 2001-06-20 | 2004-03-31 | Nuevolution A/S | Nucleoside derivatives for library preparation |
| IL163822A0 (en) | 2002-03-15 | 2005-12-18 | Nuevolution As | An improved method for synthesising templated molecules |
| AU2003247266A1 (en) | 2002-08-01 | 2004-02-23 | Nuevolution A/S | Multi-step synthesis of templated molecules |
| DK2348125T3 (en) | 2002-10-30 | 2017-10-02 | Nuevolution As | Process for the synthesis of a bifunctional complex |
| DE60330406D1 (de) | 2002-12-19 | 2010-01-14 | Nuevolution As | Durch quasizufallsstrukturen und funktionen geführte synthesemethode |
| EP1597395A2 (en) | 2003-02-21 | 2005-11-23 | Nuevolution A/S | Method for producing second-generation library |
| DK1670939T3 (da) | 2003-09-18 | 2010-03-01 | Nuevolution As | Fremgangsmåde til opnåelse af strukturel information om et kodet molekyle og fremgangsmåde til udvælgelse af forbindelser |
| JP2008541698A (ja) * | 2005-05-03 | 2008-11-27 | アンサンブル ディスカバリー コーポレイション | 核酸検出のためのターンオーバープローブおよびその使用 |
| SI3018206T1 (sl) | 2005-12-01 | 2022-02-28 | Nuevolution A/S | Postopki encimskega kodiranja za učinkovito sintezo velikih knjižnic |
| ATE544870T1 (de) * | 2006-09-18 | 2012-02-15 | Ensemble Therapeutics Corp | Profilierung einer rezeptorfamilie |
| AU2007314516A1 (en) * | 2006-09-28 | 2008-05-08 | Ensemble Discovery Corporation | Compositions and methods for biodetection by nucleic acid-templated chemistry |
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
| RS54482B1 (sr) | 2010-04-05 | 2016-06-30 | Prognosys Biosciences, Inc. | Biološka testiranja sa prostornim kodiranjem |
| EP2558577B1 (en) | 2010-04-16 | 2018-12-12 | Nuevolution A/S | Bi-functional complexes and methods for making and using such complexes |
| US20130035259A1 (en) | 2010-11-22 | 2013-02-07 | The University Of Chicago | Methods and/or Systems Producing and Providing Sets of Oligonucleotide Conjugates for Assays and Detections |
| US20140194383A1 (en) * | 2011-04-07 | 2014-07-10 | Cornell University | Monomers capable of dimerizing in an aqueous solution, and methods of using same |
| US20140296181A1 (en) * | 2011-04-07 | 2014-10-02 | Coferon, Inc. | Methods of modulating oncogenic fusion proteins |
| JP2014511865A (ja) * | 2011-04-07 | 2014-05-19 | コーネル ユニバーシティー | 水溶液中で多量化できるシリル単量体、およびその使用法 |
| US20140161729A1 (en) * | 2011-04-07 | 2014-06-12 | Cornell University | Cofluorons and methods of making and using them |
| US20120258871A1 (en) | 2011-04-08 | 2012-10-11 | Prognosys Biosciences, Inc. | Peptide constructs and assay systems |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| JP2013074824A (ja) * | 2011-09-30 | 2013-04-25 | Toyobo Co Ltd | Ｍｄｒ１遺伝子の多型を検出するためのプローブ |
| AU2013240090B2 (en) | 2012-03-27 | 2017-01-05 | Ventana Medical Systems, Inc. | Signaling conjugates and methods of use |
| WO2013177046A1 (en) | 2012-05-21 | 2013-11-28 | Solulink, Inc. | Methods and/or use of oligonucleotide conjugates for suppressing background due to cross-hybridization |
| USRE50065E1 (en) | 2012-10-17 | 2024-07-30 | 10X Genomics Sweden Ab | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| EP4219750A3 (en) * | 2013-03-11 | 2023-12-13 | Meso Scale Technologies, LLC. | Improved methods for conducting multiplexed assays |
| CA2901513C (en) * | 2013-03-12 | 2020-12-29 | Ventana Medical Systems, Inc. | Proximity assay for in situ detection of targets |
| EP3736573A1 (en) | 2013-03-15 | 2020-11-11 | Prognosys Biosciences, Inc. | Methods for detecting peptide/mhc/tcr binding |
| CN105849275B (zh) | 2013-06-25 | 2020-03-17 | 普罗格诺西斯生物科学公司 | 检测样品中生物靶标的空间分布的方法和系统 |
| US10288608B2 (en) * | 2013-11-08 | 2019-05-14 | Prognosys Biosciences, Inc. | Polynucleotide conjugates and methods for analyte detection |
| IL282614B2 (en) | 2015-02-06 | 2024-02-01 | Cell Idx Inc | Antigen-conjugated immune reagents |
| HK1250245A1 (zh) | 2015-04-10 | 2018-12-07 | Spatial Transcriptomics Ab | 生物样本的空间区别、多重核酸分析 |
| WO2016183359A1 (en) | 2015-05-12 | 2016-11-17 | Blinkbio, Inc. | Silicon based drug conjugates and methods of using same |
| CN107923915B (zh) | 2015-08-28 | 2021-05-04 | 文塔纳医疗系统公司 | 使用经掩蔽的半抗原在福尔马林固定石蜡包埋的组织中的蛋白质邻近测定 |
| CN109475567A (zh) | 2016-06-09 | 2019-03-15 | 布兰克生物股份有限公司 | 基于硅烷醇的治疗有效载荷 |
| WO2018208755A1 (en) * | 2017-05-09 | 2018-11-15 | The Regents Of The University Of California | Compositions and methods for tagging target proteins in proximity to a nucleotide sequence of interest |
| WO2019152391A1 (en) * | 2018-01-30 | 2019-08-08 | Rush University Medical Center | Sequential staining for multiplex analyses of tissues and cells |
| WO2020139848A1 (en) * | 2018-12-28 | 2020-07-02 | Becton, Dickinson And Company | Methods for spectrally resolving fluorophores of a sample and systems for same |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| CN110082524B (zh) * | 2019-04-29 | 2022-06-14 | 上海大学 | 检测脂多糖用荧光传感器、其制备方法和应用 |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| US11768175B1 (en) | 2020-03-04 | 2023-09-26 | 10X Genomics, Inc. | Electrophoretic methods for spatial analysis |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE60213826T3 (de) * | 2001-03-19 | 2013-10-17 | President And Fellows Of Harvard College | Entwicklung neuer molekularer funktionen |
| JP4657919B2 (ja) * | 2002-08-19 | 2011-03-23 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 進化する新しい分子機能 |
| US7662554B2 (en) * | 2002-10-09 | 2010-02-16 | The Trustees Of Boston University | Nucleic acid supported protein complementation |
| US8017323B2 (en) * | 2003-03-26 | 2011-09-13 | President And Fellows Of Harvard College | Free reactant use in nucleic acid-templated synthesis |
| JP2008541698A (ja) * | 2005-05-03 | 2008-11-27 | アンサンブル ディスカバリー コーポレイション | 核酸検出のためのターンオーバープローブおよびその使用 |
| US20070154899A1 (en) * | 2005-05-26 | 2007-07-05 | Coull James M | Biodetection by nucleic acid-templated chemistry |
| US7795009B2 (en) * | 2005-06-15 | 2010-09-14 | Saint Louis University | Three-component biosensors for detecting macromolecules and other analytes |
| JP2009506025A (ja) * | 2005-08-26 | 2009-02-12 | 国立大学法人京都大学 | 抗ウイルス剤及びウイルス複製阻害剤 |
| ATE544870T1 (de) * | 2006-09-18 | 2012-02-15 | Ensemble Therapeutics Corp | Profilierung einer rezeptorfamilie |
| AU2007314516A1 (en) * | 2006-09-28 | 2008-05-08 | Ensemble Discovery Corporation | Compositions and methods for biodetection by nucleic acid-templated chemistry |
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2008
- 2008-07-21 CN CN2008801092681A patent/CN101952719A/zh active Pending
- 2008-07-21 AU AU2008282557A patent/AU2008282557A1/en not_active Abandoned
- 2008-07-21 US US12/176,798 patent/US20100159446A1/en not_active Abandoned
- 2008-07-21 EP EP08796373A patent/EP2191268A2/en not_active Withdrawn
- 2008-07-21 JP JP2010518318A patent/JP2010534836A/ja not_active Withdrawn
- 2008-07-21 WO PCT/US2008/070643 patent/WO2009018003A2/en not_active Ceased
- 2008-07-21 CA CA2730565A patent/CA2730565A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| AU2008282557A1 (en) | 2009-02-05 |
| JP2010534836A (ja) | 2010-11-11 |
| WO2009018003A3 (en) | 2009-07-16 |
| CN101952719A (zh) | 2011-01-19 |
| US20100159446A1 (en) | 2010-06-24 |
| WO2009018003A2 (en) | 2009-02-05 |
| EP2191268A2 (en) | 2010-06-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |
Effective date: 20130723 |