CA2669304C - Use of a composition contaning human umbilical cord blood-derived mesenchymal stem cell for inducing differentiation and proliferation of neural precursor cells or neural stem cells to neural cells - Google Patents
Use of a composition contaning human umbilical cord blood-derived mesenchymal stem cell for inducing differentiation and proliferation of neural precursor cells or neural stem cells to neural cells Download PDFInfo
- Publication number
- CA2669304C CA2669304C CA2669304A CA2669304A CA2669304C CA 2669304 C CA2669304 C CA 2669304C CA 2669304 A CA2669304 A CA 2669304A CA 2669304 A CA2669304 A CA 2669304A CA 2669304 C CA2669304 C CA 2669304C
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- Prior art keywords
- cells
- neural
- stem cells
- umbilical cord
- cord blood
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Classifications
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- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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- A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
- A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
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- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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- A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
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- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/03—Coculture with; Conditioned medium produced by non-embryonic pluripotent stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1352—Mesenchymal stem cells
- C12N2502/1358—Bone marrow mesenchymal stem cells (BM-MSC)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Neurology (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
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- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Neurosurgery (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Epidemiology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Physical Education & Sports Medicine (AREA)
- Psychology (AREA)
- Virology (AREA)
- Hematology (AREA)
- Reproductive Health (AREA)
- Immunology (AREA)
- Heart & Thoracic Surgery (AREA)
- Hospice & Palliative Care (AREA)
- Cardiology (AREA)
- Psychiatry (AREA)
- Pain & Pain Management (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US86787506P | 2006-11-30 | 2006-11-30 | |
| US60/867,875 | 2006-11-30 | ||
| KR1020060120479A KR100959995B1 (ko) | 2006-12-01 | 2006-12-01 | 인간 제대혈 유래 간엽 줄기세포를 유효성분으로 포함하는,신경전구세포 또는 신경줄기세포의 신경세포로의 분화 및증식 유도용 조성물 |
| KR10-2006-0120479 | 2006-12-01 | ||
| PCT/KR2007/006084 WO2008066330A1 (en) | 2006-11-30 | 2007-11-29 | Use of a composition contaning human umbilical cord blood-derived mesenchymal stem cell for inducing differentiation and proliferation of neural precursor cells or neural stem cells to neural cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2669304A1 CA2669304A1 (en) | 2008-06-05 |
| CA2669304C true CA2669304C (en) | 2016-10-25 |
Family
ID=39468070
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2669304A Active CA2669304C (en) | 2006-11-30 | 2007-11-29 | Use of a composition contaning human umbilical cord blood-derived mesenchymal stem cell for inducing differentiation and proliferation of neural precursor cells or neural stem cells to neural cells |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US9387226B2 (enExample) |
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Families Citing this family (19)
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| KR20110050521A (ko) | 2008-08-20 | 2011-05-13 | 안트로제네시스 코포레이션 | 개선된 세포 조성물 및 그의 제조 방법 |
| CN101831401A (zh) * | 2010-04-23 | 2010-09-15 | 天津昂赛细胞基因工程有限公司 | 体外诱导间充质干细胞分化为神经干细胞的方法 |
| US11286463B2 (en) | 2012-03-08 | 2022-03-29 | Advanced ReGen Medical Technologies, LLC | Reprogramming of aged adult stem cells |
| KR20160018482A (ko) * | 2013-04-09 | 2016-02-17 | 어드밴스드 리젠 메디컬 테크놀로지스, 엘엘씨 | 세포 회복을 위한 조성물 및 그의 제조 및 사용 방법 |
| PL3083671T3 (pl) | 2013-12-20 | 2021-03-22 | Fred Hutchinson Cancer Center | Znakowane chimeryczne cząsteczki efektorowe i ich receptory |
| US10772911B2 (en) | 2013-12-20 | 2020-09-15 | Advanced ReGen Medical Technologies, LLC | Cell free compositions for cellular restoration and methods of making and using same |
| CR20160307A (es) | 2013-12-20 | 2016-11-08 | Advanced Regen Medical Tech Llc | Composiciones para la restauración de células y métodos para la preparación y utilización de las mismas |
| KR101808762B1 (ko) * | 2014-10-29 | 2017-12-14 | 차의과학대학교 산학협력단 | C3 또는 C1r 보체를 분비하는 태반 유래 세포 및 이를 포함하는 조성물 |
| WO2016176652A2 (en) | 2015-04-29 | 2016-11-03 | Fred Hutchinson Cancer Research Center | Modified stem cells and uses thereof |
| TWI589698B (zh) | 2015-07-22 | 2017-07-01 | 中國醫藥大學 | 間質幹細胞、其純株化擴張之方法、其分離方法及其應用 |
| US11110129B2 (en) | 2015-07-22 | 2021-09-07 | China Medical University | Method for treating multiple sclerosis |
| CN106701668B (zh) * | 2015-07-22 | 2020-12-29 | 中国医药大学 | 间质干细胞、其纯株化扩张的方法及其应用 |
| ITUB20159750A1 (it) * | 2015-12-30 | 2017-06-30 | Alfredo Gorio | Metodo per la promozione ed il miglioramento delle proprieta? del tessuto adiposo, tessuto e cellule ottenute tramite detto metodo |
| JP7101367B2 (ja) | 2016-04-28 | 2022-07-15 | 北海道公立大学法人 札幌医科大学 | シナプス形成剤 |
| KR20180130589A (ko) | 2016-04-29 | 2018-12-07 | 어드밴스드 리젠 메디컬 테크놀로지스, 엘엘씨 | 마이크로rna 조성물 및 이의 제조 및 사용 방법 |
| EP3464565A4 (en) * | 2016-05-25 | 2020-01-01 | Terumo BCT, Inc. | CELL EXPANSION |
| WO2019143847A1 (en) | 2018-01-18 | 2019-07-25 | Advanced ReGen Medical Technologies, LLC | Therapeutic compositions and methods of making and using the same |
| WO2019237106A1 (en) * | 2018-06-08 | 2019-12-12 | University Of Virginia Patent Foundation | Compositions and methods for treating stroke |
| WO2022004822A1 (ja) * | 2020-06-30 | 2022-01-06 | 国立大学法人高知大学 | 胎児付属物由来組織細胞及び／又は臍帯血細胞を培養するための海洋深層水を含む培地 |
Family Cites Families (23)
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| US7354588B1 (en) | 1987-05-04 | 2008-04-08 | Dana Farber Cancer Institute | Functional derivatives of ICAM-1 with altered ability to bind LFA-1 or HRV |
| US5733542A (en) | 1990-11-16 | 1998-03-31 | Haynesworth; Stephen E. | Enhancing bone marrow engraftment using MSCS |
| US5837576A (en) | 1997-10-31 | 1998-11-17 | Vanguard International Semiconductor Corporation | Method for forming a capacitor using a silicon oxynitride etching stop layer |
| US6900299B1 (en) | 1999-03-11 | 2005-05-31 | University Of South Florida | Interrupting the interaction of intercellular adhesion molecule-1 and respiratory syncytial virus for prevention and treatment of infection |
| CA2363269C (en) | 1999-03-12 | 2012-06-12 | The Board Of Trustees Of The Leland Stanford Junior University | Dna vaccination for treatment of autoimmune disease |
| US6372494B1 (en) | 1999-05-14 | 2002-04-16 | Advanced Tissue Sciences, Inc. | Methods of making conditioned cell culture medium compositions |
| US20040208858A1 (en) | 2000-04-12 | 2004-10-21 | Gihan Tennekoon | Therapeutic uses for mesenchymal stromal cells |
| EP1302534A4 (en) | 2000-06-26 | 2004-06-16 | Renomedix Inst Inc | CELL FRACTION WITH CELLS WHICH ARE ABLE TO DIFFERENTIATE IN NERVOUS CELLS |
| US20040037775A1 (en) | 2000-08-01 | 2004-02-26 | Siahaan Teruna J. | Leukocyte internalized peptide-drug conjugates |
| US7560280B2 (en) | 2000-11-03 | 2009-07-14 | Kourion Therapeutics Gmbh | Human cord blood derived unrestricted somatic stem cells (USSC) |
| KR100449141B1 (ko) | 2001-04-19 | 2004-09-21 | (주)라이프코드 | 간엽 간세포를 신경세포로 분화시키는 방법 |
| CN1281739C (zh) | 2002-02-19 | 2006-10-25 | 美迪宝斯特有限公司 | 从脐带血中分离并扩大培养间充质干细胞/祖细胞及将脐带血来源的间充质干细胞/祖细胞分化成各种间充质组织的方法 |
| US20090192105A1 (en) | 2002-02-20 | 2009-07-30 | Sirna Therapeutics, Inc. | RNA INTERFERENCE MEDIATED INHIBITION OF INTERCELLULAR ADHESION MOLECULE (ICAM) GENE EXPRESSION USING SHORT INTERFERING NUCELIC ACID (siNA) |
| AU2003252559A1 (en) | 2002-08-17 | 2004-03-03 | Hae-Young Suh | A method for transdifferentiating mesenchymal stem cells into neuronal cells |
| US20040203142A1 (en) | 2003-04-14 | 2004-10-14 | Reliance Life Sciences Pvt. Ltd. | Growth of neural precursor cells using umbilical cord blood serum and a process for the preparation thereof for therapeutic purposes |
| EP1674566B1 (en) | 2003-09-09 | 2011-04-13 | Keio University | Method of promoting subsistence and/or proliferation of neural stem cell and promoting extension of neurite, promoter therefor, pharmaceutical composition containing neural stem cell, method of assay and method of screening |
| US20060094064A1 (en) | 2003-11-19 | 2006-05-04 | Sandip Ray | Methods and compositions for diagnosis, stratification, and monitoring of alzheimer's disease and other neurological disorders in body fluids |
| EP1767617A1 (en) | 2005-09-26 | 2007-03-28 | Letizia Mazzini | Mesenchymal stem cells isolation and expansion method and uses thereof |
| EP1951665A1 (en) | 2005-11-14 | 2008-08-06 | Dominion Pharmakine S.L. | Novel inhibitors of the lfa-1/icam-1 interaction, and uses thereof |
| US20070253931A1 (en) | 2006-01-12 | 2007-11-01 | Osiris Therapeutics, Inc. | Use of mesenchymal stem cells for treating genetic diseases and disorders |
| KR100792184B1 (ko) | 2006-05-12 | 2008-01-07 | 재단법인서울대학교산학협력재단 | 개의 제대혈, 태반 및 개 태아의 심장 유래 다분화능 성체줄기세포, 그 제조방법 및 이를 함유하는 세포 치료제 |
| KR20080049562A (ko) | 2006-11-30 | 2008-06-04 | 가톨릭대학교 산학협력단 | 제대혈 유래 간엽 줄기세포를 유효성분으로 포함하는 신경손상 질환 치료제 |
| KR20100054711A (ko) | 2008-11-14 | 2010-05-25 | 메디포스트(주) | 간엽 줄기세포 또는 이의 배양액을 포함하는 신경질환의 예방 또는 치료용 조성물 |
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| EP2099901A1 (en) | 2009-09-16 |
| CN101541954A (zh) | 2009-09-23 |
| EP2099901B1 (en) | 2015-09-09 |
| CA2669304A1 (en) | 2008-06-05 |
| AU2007326171B2 (en) | 2012-11-15 |
| US9387226B2 (en) | 2016-07-12 |
| JP2010511381A (ja) | 2010-04-15 |
| MX2009005496A (es) | 2009-06-03 |
| US20100074875A1 (en) | 2010-03-25 |
| JP5871865B2 (ja) | 2016-03-01 |
| US9439931B2 (en) | 2016-09-13 |
| WO2008066330A1 (en) | 2008-06-05 |
| EP2099901A4 (en) | 2010-01-13 |
| WO2008066330A8 (en) | 2009-05-28 |
| CN101541954B (zh) | 2012-12-26 |
| US20130330306A1 (en) | 2013-12-12 |
| HK1134688A1 (en) | 2010-05-07 |
| JP2013226159A (ja) | 2013-11-07 |
| AU2007326171A1 (en) | 2008-06-05 |
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