CA2652118A1 - Methode d'extraction de proteines a partir de cellules - Google Patents

Methode d'extraction de proteines a partir de cellules Download PDF

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Publication number
CA2652118A1
CA2652118A1 CA002652118A CA2652118A CA2652118A1 CA 2652118 A1 CA2652118 A1 CA 2652118A1 CA 002652118 A CA002652118 A CA 002652118A CA 2652118 A CA2652118 A CA 2652118A CA 2652118 A1 CA2652118 A1 CA 2652118A1
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CA
Canada
Prior art keywords
protein
hpv
cells
reagent
intermediate composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002652118A
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English (en)
Other versions
CA2652118C (fr
Inventor
Stephen Lovell
Lydia Blank
Virginia Crews
Nancy Hasse
Peter Lu
Johannes Schweizer
John Mantlo
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Becton Dickinson and Co
Arbor Vita Corp
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Individual
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Publication of CA2652118A1 publication Critical patent/CA2652118A1/fr
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Publication of CA2652118C publication Critical patent/CA2652118C/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/82—Translation products from oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20051—Methods of production or purification of viral material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/025—Papovaviridae, e.g. papillomavirus, polyomavirus, SV40, BK virus, JC virus

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Engineering & Computer Science (AREA)
  • Oncology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention a pour objet des méthodes de production d'un extrait protéinique à partir de cellules, par exemple de cellules contenant des protéines virales. Les méthodes impliquent globalement : l'augmentation du pH des cellules jusqu'à une valeur de pH d'au moins environ 10,0 pour produire une composition intermédiaire, puis, en présence d'un détergent non ionique, la neutralisation du pH de la composition intermédiaire pour obtenir l'extrait protéinique. La présente invention concerne également des kits et des compositions permettant l'application des méthodes selon l'invention.
CA2652118A 2006-05-11 2007-05-11 Methode d'extraction de proteines a partir de cellules Active CA2652118C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US74707606P 2006-05-11 2006-05-11
US60/747,076 2006-05-11
PCT/US2007/068809 WO2007134252A1 (fr) 2006-05-11 2007-05-11 Méthode d'extraction de protéines à partir de cellules

Publications (2)

Publication Number Publication Date
CA2652118A1 true CA2652118A1 (fr) 2007-11-22
CA2652118C CA2652118C (fr) 2015-07-07

Family

ID=38694244

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2652118A Active CA2652118C (fr) 2006-05-11 2007-05-11 Methode d'extraction de proteines a partir de cellules

Country Status (8)

Country Link
US (2) US8962262B2 (fr)
EP (1) EP2024520B1 (fr)
JP (2) JP2009536958A (fr)
CN (2) CN104497098A (fr)
AU (1) AU2007249242B2 (fr)
CA (1) CA2652118C (fr)
ES (1) ES2462040T3 (fr)
WO (1) WO2007134252A1 (fr)

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US9207240B2 (en) * 2007-11-14 2015-12-08 Arbor Vita Corporation Method of efficient extraction of protein from cells
US8877436B2 (en) 2008-10-27 2014-11-04 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay on an automated platform
US20100216147A1 (en) * 2009-01-28 2010-08-26 Qiagen Gaithersburg, Inc. Sequence-specific large volume sample preparation method and assay
WO2010127326A1 (fr) * 2009-04-30 2010-11-04 Becton, Dickinson And Company Peptides de liaison e6 pour une utilisation en purification par affinité
WO2010127324A2 (fr) * 2009-04-30 2010-11-04 Arbor Vita Corporation Protéine contenant un domaine pdz chimérique pour une détection virale
CA2760542A1 (fr) 2009-05-01 2010-11-04 Qiagen Gaithersburg, Inc. Procede d'amplification non ciblee pour la detection de formes d'epissage d'arn dans un echantillon
EP2529031B1 (fr) 2010-01-29 2014-07-09 QIAGEN Gaithersburg, Inc. Procédé de détermination de confirmation de la présence de hpv dans un echantillon
EP2528932B1 (fr) * 2010-01-29 2016-11-30 QIAGEN Gaithersburg, Inc. Procédés et compositions pour la purification et l'analyse multiplexée d'acides nucléiques séquence-spécifique
CA2799200A1 (fr) 2010-05-19 2011-11-24 Qiagen Gaithersburg, Inc. Procedes et compositions pour purification sequence-specifique et analyse multiplex d'acides nucleiques
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes
JP2014511182A (ja) 2011-02-24 2014-05-15 キアゲン ガイサーズバーグ アイエヌシー． Ｈｐｖ核酸を検出するための材料及び方法
US10254279B2 (en) 2013-03-29 2019-04-09 Nima Labs, Inc. System and method for detection of target substances
US10466236B2 (en) 2013-03-29 2019-11-05 Nima Labs, Inc. System and method for detecting target substances
CN105209912B (zh) 2013-03-29 2018-04-17 第6感传感器实验室公司 用于有害物质的检测的便携式装置
CN103461320B (zh) * 2013-08-19 2015-09-02 安徽信灵检验医学科技有限公司 一种用于液基薄层脱落细胞的不等张保存液
JP2019523863A (ja) * 2016-05-31 2019-08-29 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ ウイルス抗原の血清学的検出方法

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Also Published As

Publication number Publication date
CN101460636A (zh) 2009-06-17
WO2007134252A1 (fr) 2007-11-22
CA2652118C (fr) 2015-07-07
JP2016104741A (ja) 2016-06-09
EP2024520B1 (fr) 2014-03-05
CN104497098A (zh) 2015-04-08
US8962262B2 (en) 2015-02-24
AU2007249242A1 (en) 2007-11-22
EP2024520A4 (fr) 2011-01-05
AU2007249242B2 (en) 2014-02-27
US9771398B2 (en) 2017-09-26
JP6877871B2 (ja) 2021-05-26
JP2009536958A (ja) 2009-10-22
EP2024520A1 (fr) 2009-02-18
ES2462040T3 (es) 2014-05-22
US20150266928A1 (en) 2015-09-24
US20070292899A1 (en) 2007-12-20

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